BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30139 (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 32 0.091 SPAC3G9.14 |sak1||transcriptional repressor Sak1|Schizosaccharom... 30 0.37 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.85 SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 25 7.9 SPBC17D1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 7.9 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 31.9 bits (69), Expect = 0.091 Identities = 17/59 (28%), Positives = 27/59 (45%) Frame = +3 Query: 243 SPSSHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSAIHQVP 419 +PS + + + AP+ H PA AP +AP +A AP++ A+ VP Sbjct: 511 TPSVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVP 569 Score = 30.3 bits (65), Expect = 0.28 Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 1/61 (1%) Frame = +3 Query: 243 SPSSHEASSYQAIH-SAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSAIHQVP 419 S +S +A+ I S P AP EAP+ H PAA A +AP A + + P Sbjct: 498 SSASQKAAQPSVITPSVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAP 557 Query: 420 A 422 + Sbjct: 558 S 558 Score = 29.5 bits (63), Expect = 0.48 Identities = 20/61 (32%), Positives = 24/61 (39%) Frame = +3 Query: 237 PCSPSSHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSAIHQV 416 P +P EA S +H P VAP AP AAP A + PA+ V Sbjct: 518 PAAPVVPEAPS---VHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSV 574 Query: 417 P 419 P Sbjct: 575 P 575 Score = 29.5 bits (63), Expect = 0.48 Identities = 15/46 (32%), Positives = 21/46 (45%) Frame = +3 Query: 285 SAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHATSAIHQVPA 422 +AP AP+ H+ P AP SA AP + ++ Q PA Sbjct: 519 AAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPA 564 Score = 28.3 bits (60), Expect = 1.1 Identities = 21/76 (27%), Positives = 30/76 (39%), Gaps = 3/76 (3%) Frame = +3 Query: 204 SYPHHPRSSYLPCSPSSHE---ASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYH 374 S P P + +P +PS H+ A + SAP AP EAP+ PA Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEAL 572 Query: 375 TAPAIHATSAIHQVPA 422 + P +VP+ Sbjct: 573 SVPQPPVAPVAPEVPS 588 Score = 28.3 bits (60), Expect = 1.1 Identities = 20/79 (25%), Positives = 27/79 (34%) Frame = +3 Query: 183 P*NPCCSSYPHHPRSSYLPCSPSSHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSA 362 P P P P P +P + E S +AP AP+ + P P A S Sbjct: 515 PQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSV 574 Query: 363 TTYHTAPAIHATSAIHQVP 419 AP ++ Q P Sbjct: 575 PQPPVAPVAPEVPSVPQPP 593 Score = 26.6 bits (56), Expect = 3.4 Identities = 18/76 (23%), Positives = 27/76 (35%) Frame = +3 Query: 192 PCCSSYPHHPRSSYLPCSPSSHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTY 371 P S P P + P PS + + + AP+ PA P + P + Sbjct: 599 PEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVV 658 Query: 372 HTAPAIHATSAIHQVP 419 AP++ A VP Sbjct: 659 PEAPSVPQPPAAPVVP 674 Score = 25.4 bits (53), Expect = 7.9 Identities = 18/71 (25%), Positives = 25/71 (35%) Frame = +3 Query: 210 PHHPRSSYLPCSPSSHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAI 389 P P + +P P A S P VAP E P+ PA + P Sbjct: 578 PVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQP 637 Query: 390 HATSAIHQVPA 422 A + +VP+ Sbjct: 638 PAAPVVPEVPS 648 Score = 25.4 bits (53), Expect = 7.9 Identities = 16/70 (22%), Positives = 23/70 (32%) Frame = +3 Query: 210 PHHPRSSYLPCSPSSHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAI 389 P P + +P P + A ++ P V P P AAP + PA+ Sbjct: 596 PVVPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAV 655 Query: 390 HATSAIHQVP 419 VP Sbjct: 656 PVVPEAPSVP 665 >SPAC3G9.14 |sak1||transcriptional repressor Sak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 766 Score = 29.9 bits (64), Expect = 0.37 Identities = 14/27 (51%), Positives = 17/27 (62%), Gaps = 2/27 (7%) Frame = +1 Query: 427 IQHQLFMKLQLFTFPPHFTP--PQLDM 501 +QH LF KL+L PPH P P LD+ Sbjct: 342 LQHSLFFKLKLKFLPPHKLPWIPSLDV 368 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.7 bits (61), Expect = 0.85 Identities = 18/67 (26%), Positives = 27/67 (40%) Frame = +3 Query: 219 PRSSYLPCSPSSHEASSYQAIHSAPTYHVAPAGHEAPTYHAAPAAHSATTYHTAPAIHAT 398 P + P +P + + Q + AP + VAP +AP A S+ AP Sbjct: 1427 PLKASQPTNPGAPSNHAPQVVPPAPMHAVAPVQPKAPGMVTNAPAPSSAPAPPAPVSQLP 1486 Query: 399 SAIHQVP 419 A+ VP Sbjct: 1487 PAVPNVP 1493 >SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 25.4 bits (53), Expect = 7.9 Identities = 10/24 (41%), Positives = 18/24 (75%) Frame = -3 Query: 507 DLHVQLRRREVRWKREQLELHEQL 436 +L L+R+E+ ++REQ+EL +L Sbjct: 66 NLKNDLKRKELEFEREQIELQRKL 89 >SPBC17D1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = +3 Query: 201 SSYPHHPRSSYLPCSPSSHEASSYQAIHSA 290 SS HH RSSY + + + SY + +A Sbjct: 163 SSSAHHSRSSYYDLATPTRSSFSYNTVGAA 192 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,910,306 Number of Sequences: 5004 Number of extensions: 62073 Number of successful extensions: 191 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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