BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30123 (443 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 80 1e-16 SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 38 0.001 SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 31 0.060 SPBC11C11.01 ||SPBC17D1.08|RNA-binding protein|Schizosaccharomyc... 27 0.98 SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 27 1.7 SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 26 2.3 SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 26 3.0 SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 25 4.0 SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 4.0 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 25 4.0 SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr 3|... 25 5.2 SPBC21C3.03 |||ABC1 kinase family protein|Schizosaccharomyces po... 25 5.2 SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc... 25 6.9 SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb... 25 6.9 SPBC1539.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 24 9.1 SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 24 9.1 >SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 80.2 bits (189), Expect = 1e-16 Identities = 34/61 (55%), Positives = 44/61 (72%) Frame = +3 Query: 255 DLRTPANQAVFRIEAGVCRLFREILTKKGFVEIHTPKIISAASISGANVFTVSYFKSSAY 434 DLRTP NQA+F I+AG+C+ FRE L F EIHTPK+ A+S G+NVF + YFK+ + Sbjct: 244 DLRTPTNQAIFDIQAGICQAFREFLLSNSFNEIHTPKMSGASSEGGSNVFKIQYFKTDGF 303 Query: 435 L 437 L Sbjct: 304 L 304 Score = 64.5 bits (150), Expect = 7e-12 Identities = 37/93 (39%), Positives = 56/93 (60%), Gaps = 8/93 (8%) Frame = +1 Query: 1 VKFTGNITRESIVDVEASVVRTAAPVESCTIKDVELVAHQIWTVSSARSQLPLQIEDAAR 180 VK+ G+I+ ESIV VE V ++ ++S T++D E+ I+ +S + LP +EDA R Sbjct: 151 VKWCGSISLESIVLVEGIVKKSPEIIKSATVQDAEIHISSIYVISPIKKNLPFLVEDAGR 210 Query: 181 PEK--------SDEPEALKIRVNQDTRLDNRVL 255 E+ + E ++ +RVN DTRLDNRVL Sbjct: 211 SEEQIRESEENAAEGDSKFVRVNLDTRLDNRVL 243 >SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces pombe|chr 2|||Manual Length = 568 Score = 37.5 bits (83), Expect = 0.001 Identities = 22/86 (25%), Positives = 34/86 (39%) Frame = +3 Query: 180 TGEE**AGSLEDPREPGHPARQSGFDLRTPANQAVFRIEAGVCRLFREILTKKGFVEIHT 359 TGEE L EP + Q +R +V ++ A R R+ E+ Sbjct: 230 TGEEAFTNRLNAEAEPSYLLDQRHLVIRGETASSVLKVRARALRAMRDTFENLKMTEVTP 289 Query: 360 PKIISAASISGANVFTVSYFKSSAYL 437 P ++ GA +F +Y+ AYL Sbjct: 290 PCMVQTQVEGGATLFKFNYYGQDAYL 315 >SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 31.5 bits (68), Expect = 0.060 Identities = 15/52 (28%), Positives = 25/52 (48%) Frame = +3 Query: 258 LRTPANQAVFRIEAGVCRLFREILTKKGFVEIHTPKIISAASISGANVFTVS 413 LRT +FR+ + + R+ + K F E + P I S+ VFT++ Sbjct: 127 LRTAKQGEIFRLRSDSLKALRQFFSSKDFTETNPPIITSSDCEGAGEVFTLT 178 >SPBC11C11.01 ||SPBC17D1.08|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 493 Score = 27.5 bits (58), Expect = 0.98 Identities = 12/38 (31%), Positives = 22/38 (57%) Frame = -1 Query: 356 VDLDEAFLREDFPEEPADPGLDPEDGLVSRRAQIKTRL 243 V++ +AFL+E F ++P D +D ++ R + K L Sbjct: 273 VEITDAFLKEFFNDQPTDDTKVYDDDYINERGEKKLSL 310 >SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1011 Score = 26.6 bits (56), Expect = 1.7 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = -2 Query: 316 KSLQTPASILKTAWLAGVRRS 254 K +Q P S+L T W+ +RRS Sbjct: 693 KVIQAPNSLLNTLWILNMRRS 713 >SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1063 Score = 26.2 bits (55), Expect = 2.3 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = -2 Query: 322 SLKSLQTPASILKTAWLAGVRRSKPD 245 ++KS QTP+SILKT + S PD Sbjct: 912 AIKSSQTPSSILKTPQSFFIPPSTPD 937 >SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1163 Score = 25.8 bits (54), Expect = 3.0 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = -1 Query: 350 LDEAFLREDFPEEPAD 303 +DEAF++ED EEP D Sbjct: 1022 MDEAFVQEDNDEEPLD 1037 >SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase Fab1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1932 Score = 25.4 bits (53), Expect = 4.0 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = -1 Query: 137 LDTVHI**ATSSTSLIVHDSTGAAVLTTEASTSTI 33 LDT+H TSST I+ DS T + TST+ Sbjct: 22 LDTLH----TSSTEQIIKDSENVVHTTLKLPTSTL 52 >SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 2609 Score = 25.4 bits (53), Expect = 4.0 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = -1 Query: 107 SSTSLIVHDSTGAAVLTTEASTSTIDSLVMLPV 9 + T +V ++TGAA T S T DSL + P+ Sbjct: 542 AETDALVSENTGAASQKTSKSYKTNDSLKVPPL 574 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 25.4 bits (53), Expect = 4.0 Identities = 13/41 (31%), Positives = 22/41 (53%) Frame = -1 Query: 329 EDFPEEPADPGLDPEDGLVSRRAQIKTRLSSRVSWFTRIFK 207 E+ EEP+ P E+ VS++ T SSR+ ++F+ Sbjct: 292 EEMNEEPSKPIEQTENNEVSQQDSSVTNTSSRILGIPQLFQ 332 >SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr 3|||Manual Length = 843 Score = 25.0 bits (52), Expect = 5.2 Identities = 12/29 (41%), Positives = 16/29 (55%), Gaps = 1/29 (3%) Frame = +2 Query: 77 SNRAQLRTW-NWWLIRYGRYRAPDPSFLC 160 SNRA ++ N W + Y + P P FLC Sbjct: 556 SNRAHVKLIDNIWTVIYFIFNVPFPVFLC 584 >SPBC21C3.03 |||ABC1 kinase family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 674 Score = 25.0 bits (52), Expect = 5.2 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -2 Query: 370 IIFGVWISTKPFFVRISLKSLQTPASILKTAWL 272 IIF I T PF IS L++ +S+ + WL Sbjct: 145 IIFSPIIITLPFIALISFLHLRSLSSLTVSIWL 177 >SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 24.6 bits (51), Expect = 6.9 Identities = 9/19 (47%), Positives = 15/19 (78%) Frame = -1 Query: 263 AQIKTRLSSRVSWFTRIFK 207 A+++ RLSS SWF+ I++ Sbjct: 571 ARLRMRLSSPDSWFSNIYR 589 >SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 840 Score = 24.6 bits (51), Expect = 6.9 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -2 Query: 313 SLQTPASILKTAWLAGVRRSKP 248 ++Q P S+L T ++ +RRS+P Sbjct: 539 TVQAPNSLLNTLFIENLRRSQP 560 >SPBC1539.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 386 Score = 24.2 bits (50), Expect = 9.1 Identities = 11/40 (27%), Positives = 19/40 (47%) Frame = -1 Query: 410 DGEHVSAANGRGRYNLRRVDLDEAFLREDFPEEPADPGLD 291 D E + + + N+ L ++F++ D P P D LD Sbjct: 227 DNERLENEHRTEKLNVNANSLGKSFVKHDIPLPPVDLKLD 266 >SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1191 Score = 24.2 bits (50), Expect = 9.1 Identities = 17/75 (22%), Positives = 31/75 (41%), Gaps = 2/75 (2%) Frame = +1 Query: 37 VDVEASVVRTAAPVESCTIKDVELVAHQIWTVSSARSQLPLQIEDAARPEKSDEPEALKI 216 VDV+ + T V +C I V + +++WT+ Q ++ P +++ Sbjct: 533 VDVQNHHIVTKKSVSNCPITYVMVYKNEMWTLDDMGKLYVWQEDEIMGLSIQSTPHSIRT 592 Query: 217 --RVNQDTRLDNRVL 255 LDNR+L Sbjct: 593 IPHATHAMVLDNRLL 607 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.315 0.128 0.362 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,687,738 Number of Sequences: 5004 Number of extensions: 32707 Number of successful extensions: 110 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 97 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 110 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 162176800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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