Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30123
(443 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 69 2e-12
At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative / as... 59 2e-09
At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative / as... 59 2e-09
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr... 29 1.1
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr... 29 1.1
At1g63260.2 68414.m07151 senescence-associated family protein si... 29 1.9
At1g63260.1 68414.m07152 senescence-associated family protein si... 29 1.9
At5g05840.1 68418.m00642 expressed protein contains Pfam profile... 28 3.3
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 28 3.3
At1g54560.1 68414.m06222 myosin, putative similar to myosin GI:4... 27 4.3
At5g64870.1 68418.m08160 expressed protein 27 7.5
At3g47940.1 68416.m05227 DNAJ heat shock protein, putative simil... 27 7.5
At3g17280.1 68416.m02209 F-box family protein contains Pfam PF00... 27 7.5
At1g21590.1 68414.m02699 protein kinase family protein contains ... 27 7.5
At1g09020.1 68414.m01006 protein kinase, putative similar to pro... 27 7.5
At5g66740.1 68418.m08413 hypothetical protein contains Pfam prof... 26 10.0
At5g39410.1 68418.m04774 expressed protein 26 10.0
At5g05100.1 68418.m00541 expressed protein 26 10.0
At3g62070.1 68416.m06974 expressed protein hypothetical protein ... 26 10.0
At3g61740.1 68416.m06923 PHD finger family protein (ATX3) contai... 26 10.0
At2g25380.1 68415.m03039 zinc finger protein-related very low si... 26 10.0
At2g15327.1 68415.m01750 expressed protein 26 10.0
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 68.5 bits (160), Expect = 2e-12
Identities = 31/54 (57%), Positives = 40/54 (74%)
Frame = +3
Query: 255 DLRTPANQAVFRIEAGVCRLFREILTKKGFVEIHTPKIISAASISGANVFTVSY 416
D+RTPANQA+FRI+ V FRE L KGF+EIHTPK+I+ +S G+ VF + Y
Sbjct: 217 DIRTPANQAIFRIQCQVQIAFREYLQSKGFLEIHTPKLIAGSSEGGSAVFRLDY 270
Score = 54.8 bits (126), Expect = 2e-08
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 4/89 (4%)
Frame = +1
Query: 1 VKFTGNITRESIVDVEASVVRTAAPVESCTIKDVELVAHQIWTVSSARSQLPLQIEDAAR 180
+KF ++RES+V++ V P+ T + VE+ +++ +S + LPL +EDAAR
Sbjct: 129 IKFVKQLSRESVVELIGVVSHPKKPLTGTT-QQVEIHVRKMYCLSRSLPNLPLVVEDAAR 187
Query: 181 P----EKSDEPEALKIRVNQDTRLDNRVL 255
EKS + RV QDTRL+NRVL
Sbjct: 188 SESDIEKSGKDGKQAARVLQDTRLNNRVL 216
>At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 58.8 bits (136), Expect = 2e-09
Identities = 28/54 (51%), Positives = 38/54 (70%)
Frame = +3
Query: 255 DLRTPANQAVFRIEAGVCRLFREILTKKGFVEIHTPKIISAASISGANVFTVSY 416
DLRTPANQA+F+++ V FRE L K FV IHTPK+++ +S G+ VF + Y
Sbjct: 243 DLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEY 296
Score = 56.8 bits (131), Expect = 6e-09
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 4/89 (4%)
Frame = +1
Query: 1 VKFTGNITRESIVDVEASVVRTAAPVESCTIKDVELVAHQIWTVSSARSQLPLQIEDAAR 180
VK+ ++RES VDV V P+ T + VE+ +++ ++ + ++LPL +EDAAR
Sbjct: 155 VKYLKQLSRESFVDVIGVVTLPKEPLTGTT-QQVEIQVRKVYCINKSLAKLPLSVEDAAR 213
Query: 181 PEKSDEPE----ALKIRVNQDTRLDNRVL 255
E E + RVNQDTRL+ RVL
Sbjct: 214 SEADIEASLQTPSPAARVNQDTRLNYRVL 242
>At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 58.8 bits (136), Expect = 2e-09
Identities = 28/54 (51%), Positives = 38/54 (70%)
Frame = +3
Query: 255 DLRTPANQAVFRIEAGVCRLFREILTKKGFVEIHTPKIISAASISGANVFTVSY 416
DLRTPANQA+F+++ V FRE L K FV IHTPK+++ +S G+ VF + Y
Sbjct: 243 DLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEY 296
Score = 56.8 bits (131), Expect = 6e-09
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 4/89 (4%)
Frame = +1
Query: 1 VKFTGNITRESIVDVEASVVRTAAPVESCTIKDVELVAHQIWTVSSARSQLPLQIEDAAR 180
VK+ ++RES VDV V P+ T + VE+ +++ ++ + ++LPL +EDAAR
Sbjct: 155 VKYLKQLSRESFVDVIGVVTLPKEPLTGTT-QQVEIQVRKVYCINKSLAKLPLSVEDAAR 213
Query: 181 PEKSDEPE----ALKIRVNQDTRLDNRVL 255
E E + RVNQDTRL+ RVL
Sbjct: 214 SEADIEASLQTPSPAARVNQDTRLNYRVL 242
>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.5 bits (63), Expect = 1.1
Identities = 22/63 (34%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = -2
Query: 295 SILKTAWLAGVRRSKPDCRAGCPGSRGSSRLPAHHSSPVWPRLRSAE----EAGIWRSIP 128
SIL W +G++ + P C G S G + A P L SAE E G W IP
Sbjct: 210 SILTNTWTSGMQMNVPRCLFG-SASLGEIAVIAGGCDPRGRILSSAELYNSETGEWTVIP 268
Query: 127 SIS 119
S++
Sbjct: 269 SMN 271
>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.5 bits (63), Expect = 1.1
Identities = 22/63 (34%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = -2
Query: 295 SILKTAWLAGVRRSKPDCRAGCPGSRGSSRLPAHHSSPVWPRLRSAE----EAGIWRSIP 128
SIL W +G++ + P C G S G + A P L SAE E G W IP
Sbjct: 210 SILTNTWTSGMQMNVPRCLFG-SASLGEIAVIAGGCDPRGRILSSAELYNSETGEWTVIP 268
Query: 127 SIS 119
S++
Sbjct: 269 SMN 271
>At1g63260.2 68414.m07151 senescence-associated family protein
similar to senescence-associated protein 5 [Hemerocallis
hybrid cultivar] gi|3551954|gb|AAC34855
Length = 258
Score = 28.7 bits (61), Expect = 1.9
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = -2
Query: 406 VNTLAPLMDAADIIFGVWIST 344
VN L L+ A IIFGVW+ST
Sbjct: 13 VNLLTMLLAVAVIIFGVWMST 33
>At1g63260.1 68414.m07152 senescence-associated family protein
similar to senescence-associated protein 5 [Hemerocallis
hybrid cultivar] gi|3551954|gb|AAC34855
Length = 284
Score = 28.7 bits (61), Expect = 1.9
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = -2
Query: 406 VNTLAPLMDAADIIFGVWIST 344
VN L L+ A IIFGVW+ST
Sbjct: 13 VNLLTMLLAVAVIIFGVWMST 33
>At5g05840.1 68418.m00642 expressed protein contains Pfam profile
PF04788: Protein of unknown function (DUF620);
expression supported by MPSS
Length = 439
Score = 27.9 bits (59), Expect = 3.3
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1
Query: 97 DVELVAHQIWTVSSARSQLPLQIEDA 174
+VE++ H +W S R+ L +Q+ED+
Sbjct: 275 NVEIIRHTVWGCFSQRTGLLIQLEDS 300
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 27.9 bits (59), Expect = 3.3
Identities = 13/45 (28%), Positives = 20/45 (44%)
Frame = +3
Query: 282 VFRIEAGVCRLFREILTKKGFVEIHTPKIISAASISGANVFTVSY 416
+FR A + R L K F+E+ TP + A + A F +
Sbjct: 279 IFRTRAKIISYVRRFLDNKNFLEVETPMMNMIAGGAAARPFVTHH 323
>At1g54560.1 68414.m06222 myosin, putative similar to myosin
GI:433663 from [Arabidopsis thaliana]
Length = 1529
Score = 27.5 bits (58), Expect = 4.3
Identities = 13/32 (40%), Positives = 15/32 (46%)
Frame = -1
Query: 320 PEEPADPGLDPEDGLVSRRAQIKTRLSSRVSW 225
PEE LDP+ L+SR KT S W
Sbjct: 383 PEEVIKRSLDPQSALISRDGLAKTIYSRLFDW 414
>At5g64870.1 68418.m08160 expressed protein
Length = 479
Score = 26.6 bits (56), Expect = 7.5
Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 1/65 (1%)
Frame = +1
Query: 19 ITRESIVDVEASVVRTAAPVESCTIKDVELVAHQIWTVSSARSQLPLQIEDA-ARPEKSD 195
IT I D++ + P +SCT+ DV V + + + +LP I + D
Sbjct: 14 ITGGGITDIKLAKKSWVFPWQSCTVFDVSPVNYTFEVQAMSSEKLPFVIPAVFTIGPRVD 73
Query: 196 EPEAL 210
+P AL
Sbjct: 74 DPHAL 78
>At3g47940.1 68416.m05227 DNAJ heat shock protein, putative similar
to SP|O89114 DnaJ homolog subfamily B member 5 (Heat
shock protein Hsp40-3) {Mus musculus}; contains Pfam
profile PF00226: DnaJ domain
Length = 350
Score = 26.6 bits (56), Expect = 7.5
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +3
Query: 45 GGFGREDGRPSRIVHN*GRGTGGSSDMDGIERQIPA 152
GG GR +R N TGG++ G R++PA
Sbjct: 137 GGSNNAGGRGNRAFRNGHFNTGGANGYSGEMRKVPA 172
>At3g17280.1 68416.m02209 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain
Length = 386
Score = 26.6 bits (56), Expect = 7.5
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = -3
Query: 162 LQRKLGSGARYRPYLMSHQFHVLNCARFDWGGRPHDRSLHVDY 34
+++KLG AR +LM+H+ +N D G P S+ +D+
Sbjct: 44 VEKKLGKAARETVFLMNHE---VNSISVDIHGIPKGYSVSMDF 83
>At1g21590.1 68414.m02699 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 756
Score = 26.6 bits (56), Expect = 7.5
Identities = 15/54 (27%), Positives = 26/54 (48%)
Frame = -1
Query: 164 ICRGSWDLALDTVHI**ATSSTSLIVHDSTGAAVLTTEASTSTIDSLVMLPVNL 3
+ + W LA+D + +S I+H S G ++A +T+ S +PV L
Sbjct: 137 VAKDCWVLAVDNGKVMFQKDGSSSIIHHSKG----KSDARRNTLSSFFQMPVTL 186
>At1g09020.1 68414.m01006 protein kinase, putative similar to
protein kinase AKINbetagamma-2 [Zea mays] GI:11139548,
AKINbetagamma-1 [Zea mays] GI:11139546; contains Pfam
profile PF00571: CBS domain
Length = 487
Score = 26.6 bits (56), Expect = 7.5
Identities = 18/51 (35%), Positives = 28/51 (54%)
Frame = -1
Query: 377 GRYNLRRVDLDEAFLREDFPEEPADPGLDPEDGLVSRRAQIKTRLSSRVSW 225
GR N+ D+D+ FLR P + A P + D +SR +I LS+R ++
Sbjct: 117 GRSNM---DVDDVFLRTADPSQEAVPRMSGVDLELSRH-RISVLLSTRTAY 163
>At5g66740.1 68418.m08413 hypothetical protein contains Pfam profile
PF04788: Protein of unknown function (DUF620)
Length = 247
Score = 26.2 bits (55), Expect = 10.0
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 103 ELVAHQIWTVSSARSQLPLQIEDA 174
E++ H IW S RS L +Q ED+
Sbjct: 135 EIIHHTIWGYFSQRSGLLIQFEDS 158
>At5g39410.1 68418.m04774 expressed protein
Length = 454
Score = 26.2 bits (55), Expect = 10.0
Identities = 17/36 (47%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = -2
Query: 370 IIFGVWISTKPFFVRISLKSLQTPASI-LKTAWLAG 266
+I G T + VR +LK LQTP+S LK+ LAG
Sbjct: 14 VILGASGFTGKYVVREALKFLQTPSSSPLKSLALAG 49
>At5g05100.1 68418.m00541 expressed protein
Length = 324
Score = 26.2 bits (55), Expect = 10.0
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +3
Query: 54 GREDGRPSRIVHN*GRGTGGSSDMDGIERQIPASSADRR 170
GR +G I RG GGS G+E+ + S +R+
Sbjct: 114 GRPEGFKIAIKPRPNRGAGGSGSGSGVEKNLLRSVEERK 152
>At3g62070.1 68416.m06974 expressed protein hypothetical protein
F14M4.23 - Arabidopsis thaliana, PIR:T02193
Length = 228
Score = 26.2 bits (55), Expect = 10.0
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = +2
Query: 38 STWRLRS*GRPPQSNRAQLRTW 103
+TWR+ S G P+ +A+L+ W
Sbjct: 193 ATWRISSPGDDPKEVKARLKVW 214
>At3g61740.1 68416.m06923 PHD finger family protein (ATX3) contains
Pfam domains PF00628: PHD-finger and PF00855: PWWP
domain; identical to cDNA trithorax 3 (ATX3) partial cds
GI:15217142
Length = 799
Score = 26.2 bits (55), Expect = 10.0
Identities = 20/67 (29%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +3
Query: 249 GFDLRTPANQA-VF-RIEAGVCRLFREILTKKGFVEIHTPKIISAASISGANVFTVSYFK 422
GF +T A Q+ VF + A CR++ TKK +E + ++ + S + + ++ FK
Sbjct: 683 GFQTQTQAEQSRVFDSLSAARCRIYSRSNTKKIDLEAISHRLKGPSHHSLSAIENLNSFK 742
Query: 423 SSAYLRA 443
+S RA
Sbjct: 743 ASFSFRA 749
>At2g25380.1 68415.m03039 zinc finger protein-related very low
similarity to SP|O95376 Ariadne-2 protein homolog
(ARI-2) (Triad1 protein) {Homo sapiens}; contains Pfam
profile PF01485: IBR (In Between Ring fingers) domain
Length = 350
Score = 26.2 bits (55), Expect = 10.0
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +1
Query: 1 VKFTGNITRESIVDVEASVVRTAAPVESCTIKDVELVAHQIW 126
VKF + RE IV +SV A E+C I E VA +++
Sbjct: 172 VKFAYRLAREEIVSKSSSVNVKEAQGETCVICLEETVADRMF 213
>At2g15327.1 68415.m01750 expressed protein
Length = 191
Score = 26.2 bits (55), Expect = 10.0
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -2
Query: 184 PVWPRLRSAEEAGIWRSIPSISDEPPVP 101
P PRLR + + W P I+D PP+P
Sbjct: 159 PSPPRLRHS--SANWNGSPMINDRPPLP 184
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.128 0.362
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,259,170
Number of Sequences: 28952
Number of extensions: 195303
Number of successful extensions: 677
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 671
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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