BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30121 (716 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 155 5e-39 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 153 2e-38 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 129 4e-31 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 95 9e-21 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 79 6e-16 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 69 5e-13 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 68 2e-12 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 53 5e-08 SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 29 0.66 SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosacchar... 28 1.2 SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|... 27 2.0 SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 27 2.7 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 4.7 SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr ... 26 4.7 SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p... 25 8.2 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 155 bits (377), Expect = 5e-39 Identities = 73/85 (85%), Positives = 79/85 (92%) Frame = +1 Query: 1 VKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSST 180 VK+TAGDTHLGGEDFD+R+VNHF+QEFKRK KKD+ N RA+RRLRTACE AKRTLSSS Sbjct: 217 VKATAGDTHLGGEDFDSRLVNHFIQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSA 276 Query: 181 QASIEIDSLFEGIDFYTSITRARFE 255 QASIEIDSLFEGIDFYTSITRARFE Sbjct: 277 QASIEIDSLFEGIDFYTSITRARFE 301 Score = 128 bits (310), Expect = 6e-31 Identities = 72/134 (53%), Positives = 85/134 (63%) Frame = +3 Query: 255 ELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSI 434 EL ADLFR TMEPVE+ LRD+K+DKA +++IVLVGGSTRIP+VQKL+ DFFNGKE KSI Sbjct: 302 ELCADLFRKTMEPVERVLRDSKVDKASVNEIVLVGGSTRIPRVQKLVSDFFNGKEPCKSI 361 Query: 435 YPDEXXXXXXXXXXXILHGDNSEEVKDXXXXXXXXXXXGIETAGSVMTTLHYSGNTNQSP 614 PDE +L GD SE+ +D GIETAG VMT L T P Sbjct: 362 NPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGIETAGGVMTPLIKRNTT--IP 419 Query: 615 T*NRLRHSTTYSDN 656 T + +TYSDN Sbjct: 420 T-KKSEIFSTYSDN 432 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 153 bits (371), Expect = 2e-38 Identities = 72/85 (84%), Positives = 78/85 (91%) Frame = +1 Query: 1 VKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSST 180 VK+TAGDTHLGGEDFD+R+VNHF QEFKRK KKD+ N RA+RRLRTACE AKRTLSSS Sbjct: 217 VKATAGDTHLGGEDFDSRLVNHFAQEFKRKNKKDITGNARAVRRLRTACERAKRTLSSSA 276 Query: 181 QASIEIDSLFEGIDFYTSITRARFE 255 QASIEIDSL+EGIDFYTSITRARFE Sbjct: 277 QASIEIDSLYEGIDFYTSITRARFE 301 Score = 127 bits (307), Expect = 1e-30 Identities = 71/134 (52%), Positives = 86/134 (64%) Frame = +3 Query: 255 ELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSI 434 EL ADLFR+TMEPVEK LRD+K+DK+ +++IVLVGGSTRIP++QKL+ DFFNGKE KSI Sbjct: 302 ELCADLFRNTMEPVEKVLRDSKIDKSSVNEIVLVGGSTRIPRIQKLVSDFFNGKEPCKSI 361 Query: 435 YPDEXXXXXXXXXXXILHGDNSEEVKDXXXXXXXXXXXGIETAGSVMTTLHYSGNTNQSP 614 PDE IL GD SE+ +D GIETAG VMT L T P Sbjct: 362 NPDEAVAYGAAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTPLIKRNTT--IP 419 Query: 615 T*NRLRHSTTYSDN 656 T + +TY+DN Sbjct: 420 T-KKSEVFSTYADN 432 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 129 bits (311), Expect = 4e-31 Identities = 60/85 (70%), Positives = 71/85 (83%) Frame = +1 Query: 1 VKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSST 180 V +TAGDTHLGGEDFDN +V HF+QEFKRK K D++ + RALRRLR+ACE AKR LSS T Sbjct: 221 VLATAGDTHLGGEDFDNALVEHFIQEFKRKQKIDISDDPRALRRLRSACERAKRALSSVT 280 Query: 181 QASIEIDSLFEGIDFYTSITRARFE 255 Q ++E+DSL GIDF +SITRARFE Sbjct: 281 QTTVEVDSLSNGIDFSSSITRARFE 305 Score = 99.1 bits (236), Expect = 5e-22 Identities = 56/140 (40%), Positives = 81/140 (57%), Gaps = 1/140 (0%) Frame = +3 Query: 255 ELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSI 434 ++NA F++T++PV K L+D+K+ KA +HDIVLVGGSTRIPKVQ+L+ DFF+G+ LNKSI Sbjct: 306 DINATTFKATIDPVAKVLKDSKVPKADVHDIVLVGGSTRIPKVQRLVSDFFDGRALNKSI 365 Query: 435 YPDEXXXXXXXXXXXIL-HGDNSEEVKDXXXXXXXXXXXGIETAGSVMTTLHYSGNTNQS 611 PDE +L + +S++ +D G+ G+V + Sbjct: 366 NPDEAVAYGAAVQAAVLTNKADSDKTQDLLLLDVVPLSLGVAMEGNVFGVV--CPRNTPI 423 Query: 612 PT*NRLRHSTTYSDNPTRST 671 PT + R TT +DN T T Sbjct: 424 PTIKK-RTFTTVADNQTTVT 442 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 95.1 bits (226), Expect = 9e-21 Identities = 56/136 (41%), Positives = 75/136 (55%) Frame = +3 Query: 255 ELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSI 434 E+N DLF+ T++PVE+ L+D+ + K++I DIVLVGGSTRIPKVQ+LL+ FF GK+ +K I Sbjct: 334 EINMDLFKKTLKPVEQVLKDSNLKKSEIDDIVLVGGSTRIPKVQELLESFF-GKKASKGI 392 Query: 435 YPDEXXXXXXXXXXXILHGDNSEEVKDXXXXXXXXXXXGIETAGSVMTTLHYSGNTNQSP 614 PDE +L G+ + + GIET G VMT L G P Sbjct: 393 NPDEAVAYGAAVQAGVLSGEEGSD--NIVLLDVIPLTLGIETTGGVMTKL--IGRNTPIP 448 Query: 615 T*NRLRHSTTYSDNPT 662 T ST + T Sbjct: 449 TRKSQIFSTAVDNQNT 464 Score = 91.5 bits (217), Expect = 1e-19 Identities = 41/85 (48%), Positives = 59/85 (69%) Frame = +1 Query: 1 VKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSST 180 V +T+GDTHLGGEDFDNR++N+ + + RK D+ + +A+ +L+ E AKRTLSS Sbjct: 249 VLATSGDTHLGGEDFDNRVINYLARTYNRKNNVDVTKDLKAMGKLKREVEKAKRTLSSQK 308 Query: 181 QASIEIDSLFEGIDFYTSITRARFE 255 IEI+S F G DF +++RA+FE Sbjct: 309 SVRIEIESFFNGQDFSETLSRAKFE 333 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 79.0 bits (186), Expect = 6e-16 Identities = 37/85 (43%), Positives = 55/85 (64%) Frame = +1 Query: 1 VKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSST 180 + ++ D +LGGE + +VN F +EF++K D N R+L +LR CE KR LS+ T Sbjct: 243 ILASVHDENLGGEQLTDVLVNFFAKEFEKKNGIDPRKNARSLAKLRAQCEITKRVLSNGT 302 Query: 181 QASIEIDSLFEGIDFYTSITRARFE 255 AS +DSL +GIDF++SI R R++ Sbjct: 303 TASAAVDSLADGIDFHSSINRLRYD 327 Score = 32.7 bits (71), Expect = 0.054 Identities = 17/72 (23%), Positives = 37/72 (51%), Gaps = 5/72 (6%) Frame = +3 Query: 231 VNYSCSLRELNADLFRSTM-----EPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLL 395 +++ S+ L DL S + V +++ A M+ I +++L GG++ PK+ L+ Sbjct: 315 IDFHSSINRLRYDLAASATLNRMADLVTEAVEKANMEPFDISEVILAGGASNTPKLTSLM 374 Query: 396 QDFFNGKELNKS 431 + F + + +S Sbjct: 375 ESIFPEQTIIRS 386 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 69.3 bits (162), Expect = 5e-13 Identities = 32/85 (37%), Positives = 51/85 (60%) Frame = +1 Query: 1 VKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSST 180 +KST D +LG + D ++++F EFK KYK D+ +N +A RL TA E K+ LS++ Sbjct: 225 IKSTVCDRNLGSRNMDKALIDYFAAEFKEKYKIDVLSNPKATFRLATAVERLKKVLSANA 284 Query: 181 QASIEIDSLFEGIDFYTSITRARFE 255 A + ++ + ID + I R+ FE Sbjct: 285 NAPLNVEMIMNDIDASSFIKRSDFE 309 Score = 44.4 bits (100), Expect = 2e-05 Identities = 23/64 (35%), Positives = 39/64 (60%) Frame = +3 Query: 255 ELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSI 434 EL L P+EK+L A + K ++ I +VGG TR+P V++++ ++F GK L+ ++ Sbjct: 310 ELIKPLLERLTPPIEKALELAGIKKEDLYSIEMVGGCTRVPIVKEVIANYF-GKGLSFTL 368 Query: 435 YPDE 446 DE Sbjct: 369 NQDE 372 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/37 (35%), Positives = 18/37 (48%) Frame = +3 Query: 228 HVNYSCSLRELNADLFRSTMEPVEKSLRDAKMDKAQI 338 H NYS S+ E + F +++L MDKA I Sbjct: 208 HSNYSVSIVEFSRGQFHIKSTVCDRNLGSRNMDKALI 244 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 67.7 bits (158), Expect = 2e-12 Identities = 32/66 (48%), Positives = 45/66 (68%) Frame = +1 Query: 1 VKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSST 180 V+ST GDTHLGGEDFD +V H V+ FK+ DL+ ++ A++R+R A E AK LSS + Sbjct: 261 VRSTNGDTHLGGEDFDVALVRHIVETFKKNEGLDLSKDRLAVQRIREAAEKAKCELSSLS 320 Query: 181 QASIEI 198 + I + Sbjct: 321 KTDISL 326 Score = 66.1 bits (154), Expect = 5e-12 Identities = 40/122 (32%), Positives = 63/122 (51%), Gaps = 3/122 (2%) Frame = +3 Query: 228 HVNYSCS---LRELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQ 398 H+N S +L L R T++P +++L+DA + ++I++++LVGG TR+P+V + ++ Sbjct: 338 HINMEISRAQFEKLVDPLVRRTIDPCKRALKDANLQTSEINEVILVGGMTRMPRVVETVK 397 Query: 399 DFFNGKELNKSIYPDEXXXXXXXXXXXILHGDNSEEVKDXXXXXXXXXXXGIETAGSVMT 578 F +E KS+ PDE +L G VKD GIET G V T Sbjct: 398 SIFK-REPAKSVNPDEAVAIGAAIQGGVLSG----HVKDLVLLDVTPLSLGIETLGGVFT 452 Query: 579 TL 584 L Sbjct: 453 RL 454 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 52.8 bits (121), Expect = 5e-08 Identities = 27/77 (35%), Positives = 42/77 (54%) Frame = +1 Query: 28 LGGEDFDNRMVNHFVQEFKRKYKKDLATNKRALRRLRTACETAKRTLSSSTQASIEIDSL 207 L G + + +++ F +K+ DL+ N RAL RL K LS++++A I+ L Sbjct: 262 LSGNEINRKLIGFMKNSFYQKHGIDLSHNHRALARLEKEALRVKHILSANSEAIASIEEL 321 Query: 208 FEGIDFYTSITRARFES 258 +GIDF ITR+ ES Sbjct: 322 ADGIDFRLKITRSVLES 338 Score = 52.8 bits (121), Expect = 5e-08 Identities = 22/66 (33%), Positives = 43/66 (65%) Frame = +3 Query: 249 LRELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNK 428 L L D+ + +EP+ K+L+ A + ++I+ I+L GG++RIP +Q L D+ + +++K Sbjct: 336 LESLCKDMEDAAVEPINKALKKANLTFSEINSIILFGGASRIPFIQSTLADYVSSDKISK 395 Query: 429 SIYPDE 446 ++ DE Sbjct: 396 NVNADE 401 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 29.1 bits (62), Expect = 0.66 Identities = 27/77 (35%), Positives = 37/77 (48%), Gaps = 4/77 (5%) Frame = -1 Query: 527 KQQQILDLLR-VVTVQDSSLDSCTISYG-LVRVYRFV--ELLSIKEILQELLHLGDTSGA 360 K QQ+LDLL +V D+ +DS + L ++ V S+ ELL LGD S Sbjct: 932 KHQQLLDLLESLVGNNDNLIDSIKTPHTELQKITDHVLKGTTSLANHTNELLGLGDESLC 991 Query: 359 THQYNIVDLSLIHLGIT 309 + I D SL+ L T Sbjct: 992 NLETTIEDTSLVKLETT 1008 >SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 28.3 bits (60), Expect = 1.2 Identities = 24/64 (37%), Positives = 33/64 (51%), Gaps = 2/64 (3%) Frame = +2 Query: 89 NTKRTSLPTRELLGVCVLHVRRQR-GPCHRPHKRALR*ILSLRVLTSTRQL-LVLASRAE 262 N K+ LP E + + + V GP H+PH R LR + LR + QL VLA+ + Sbjct: 9 NQKKNVLPKPEDIQLIICDVDGTLLGPDHKPHPRNLRALKYLR--ENYPQLPFVLATGRQ 66 Query: 263 RRSV 274 R SV Sbjct: 67 RTSV 70 >SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|chr 3|||Manual Length = 700 Score = 27.5 bits (58), Expect = 2.0 Identities = 10/31 (32%), Positives = 20/31 (64%) Frame = +3 Query: 216 Y*LLHVNYSCSLRELNADLFRSTMEPVEKSL 308 Y +HVN+S S + + D++R +P+E ++ Sbjct: 297 YERIHVNFSLSSYQQDYDIYRKPTDPIEVTI 327 >SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism |Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 27.1 bits (57), Expect = 2.7 Identities = 16/63 (25%), Positives = 26/63 (41%) Frame = +1 Query: 235 ITRARFES*TPICSGLPWSQWRSLSVMPRWIRLKSTILYWWVAPLVSPRCRSSCKISLME 414 I AR E TP + W+ R SV+P+ + + + PL + I ++ Sbjct: 240 IANARGEGITPANIAISWAVKRGTSVLPKSVNESRIVSNFLYIPLTDKEMEAINNIGVVR 299 Query: 415 RSS 423 R S Sbjct: 300 RFS 302 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 4.7 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -3 Query: 342 RGFEPYPSWHHGETSPLAP 286 + +P P+ HG+TSPL+P Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438 >SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr 3|||Manual Length = 384 Score = 26.2 bits (55), Expect = 4.7 Identities = 12/27 (44%), Positives = 15/27 (55%) Frame = -1 Query: 83 LNSWTKWLTMRLSKSSPPKWVSPAVDF 3 LN K++ SK P W+SPA DF Sbjct: 256 LNYKNKFVNAWQSKVGPLPWMSPATDF 282 >SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 4/34 (11%) Frame = +1 Query: 268 ICSGLPWSQ----WRSLSVMPRWIRLKSTILYWW 357 ICS LP + W + S PR+ R S ++ WW Sbjct: 133 ICSALPAAGSIYLWAAESAGPRFGRFVSFLVAWW 166 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,099,970 Number of Sequences: 5004 Number of extensions: 62837 Number of successful extensions: 219 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 216 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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