Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30118
(788 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g27060.1 68416.m03385 ribonucleoside-diphosphate reductase sm... 182 2e-46
At3g23580.1 68416.m02966 ribonucleoside-diphosphate reductase sm... 165 2e-41
At5g40942.1 68418.m04973 ribonucleoside-diphosphate reductase sm... 91 1e-18
At3g11310.1 68416.m01375 hypothetical protein 30 1.5
At1g04860.1 68414.m00482 ubiquitin-specific protease 2 (UBP2) id... 29 3.5
At5g47150.1 68418.m05812 YDG/SRA domain-containing protein low s... 29 4.6
At1g49740.1 68414.m05578 expressed protein similar to MAP3K-like... 29 4.6
At5g21930.1 68418.m02545 ATPase E1-E2 type family protein / halo... 28 6.1
At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative... 28 6.1
At1g53140.1 68414.m06017 dynamin family protein low similarity t... 28 6.1
>At3g27060.1 68416.m03385 ribonucleoside-diphosphate reductase small
chain, putative / ribonucleotide reductase, putative
similar to ribonucleotide reductase R2 [Nicotiana
tabacum] GI:1044912; contains Pfam profile PF00268:
Ribonucleotide reductase, small chain
Length = 332
Score = 182 bits (444), Expect = 2e-46
Identities = 115/248 (46%), Positives = 140/248 (56%), Gaps = 1/248 (0%)
Frame = +3
Query: 3 FIKHVLAFFAASDGIVNENLVERFSQEVQVTEARCFYGFQIAMENVHSEMYSLLIDTYIR 182
FIKHVLAFFAASDGIV ENL RF +VQV+EAR FYGFQIA+EN+HSEMYSLL+DTYI+
Sbjct: 64 FIKHVLAFFAASDGIVLENLASRFMSDVQVSEARAFYGFQIAIENIHSEMYSLLLDTYIK 123
Query: 183 DPKREIFCLMRLRLFPV*RRKQIGSTVDCKQDCNIW*KDCCLCSCRRNLFLR-QFCFHLL 359
D K + P +K + + + + +C +F FC
Sbjct: 124 DNKERDHLFRAIETIPCVAKKAQWA-MKWIDGSQTFAERIIAFACVEGIFFSGSFCSIFW 182
Query: 360 AQKAGPYAGTYIQ**TYFQG*GSAYRFCLSDVPTLSSKAN*GMCPAYYKSAVVIEQEFLT 539
+K G G + G F L +K + + AV IE+EF+
Sbjct: 183 LKKRGLMPGLTFSNELISRDEGLHCDFACLLYTLLKTKLSEERVKSIVCDAVEIEREFVC 242
Query: 540 DALPVRLLGMNCELMSQYIEFVADRLLVDLIGGKHYNTKNPFGLS*ILISAGKGKTNFFE 719
DALP L+GMN +LMSQYIEFVADRLL L GK Y NPF LIS +GKTNFFE
Sbjct: 243 DALPCALVGMNRDLMSQYIEFVADRLLGALGYGKVYGVTNPFDWM-ELISL-QGKTNFFE 300
Query: 720 KKVGDYQK 743
K+VGDYQK
Sbjct: 301 KRVGDYQK 308
Score = 94.3 bits (224), Expect = 8e-20
Identities = 47/89 (52%), Positives = 60/89 (67%)
Frame = +1
Query: 238 EESRLGLQWIASKTATFGERIVAFAAVEXXXXXXXXXXXXWLKKRGLMPGLTFSNELISR 417
++++ ++WI + TF ERI+AFA VE WLKKRGLMPGLTFSNELISR
Sbjct: 143 KKAQWAMKWIDG-SQTFAERIIAFACVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISR 201
Query: 418 DEGLHTDFACLMFQHLVQKPTKECVLHII 504
DEGLH DFACL++ L K ++E V I+
Sbjct: 202 DEGLHCDFACLLYTLLKTKLSEERVKSIV 230
Score = 40.3 bits (90), Expect = 0.001
Identities = 15/23 (65%), Positives = 19/23 (82%)
Frame = +2
Query: 185 SEERDFLFNAVETLPCVKKKADW 253
++ERD LF A+ET+PCV KKA W
Sbjct: 125 NKERDHLFRAIETIPCVAKKAQW 147
>At3g23580.1 68416.m02966 ribonucleoside-diphosphate reductase small
chain / ribonucleotide reductase nearly identical to
SP|P50651 Ribonucleoside-diphosphate reductase small
chain (EC 1.17.4.1) (Ribonucleotide reductase) (R2
subunit) {Arabidopsis thaliana}
Length = 341
Score = 165 bits (402), Expect = 2e-41
Identities = 106/248 (42%), Positives = 133/248 (53%), Gaps = 1/248 (0%)
Frame = +3
Query: 3 FIKHVLAFFAASDGIVNENLVERFSQEVQVTEARCFYGFQIAMENVHSEMYSLLIDTYIR 182
FI H+LAFFAASDGIV ENL RF +VQV EAR FYGFQIAMEN+HSEMYSLL++T+I+
Sbjct: 75 FISHILAFFAASDGIVLENLAARFLNDVQVPEARAFYGFQIAMENIHSEMYSLLLETFIK 134
Query: 183 DPKREIFCLMRLRLFPV*RRKQIGSTVDCKQDCNIW*KDCCLCSCRRNLFLR-QFCFHLL 359
D K + + P K+ +D Q + +C +F FC
Sbjct: 135 DSKEKDRLFNAIETIPC-ISKKAKWCLDWIQSPMSFAVRLVAFACVEGIFFSGSFCAIFW 193
Query: 360 AQKAGPYAGTYIQ**TYFQG*GSAYRFCLSDVPTLSSKAN*GMCPAYYKSAVVIEQEFLT 539
+K G G + G F L + AV IE EF+
Sbjct: 194 LKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLQKQLPLEKVYQIVHEAVEIETEFVC 253
Query: 540 DALPVRLLGMNCELMSQYIEFVADRLLVDLIGGKHYNTKNPFGLS*ILISAGKGKTNFFE 719
ALP L+GMN LMSQYI+FVADRLLV L + Y +NPF + + +GKTNFFE
Sbjct: 254 KALPCDLIGMNSNLMSQYIQFVADRLLVTLGCERTYKAENPFDWMEFI--SLQGKTNFFE 311
Query: 720 KKVGDYQK 743
K+VG+YQK
Sbjct: 312 KRVGEYQK 319
Score = 88.6 bits (210), Expect = 4e-18
Identities = 46/83 (55%), Positives = 53/83 (63%)
Frame = +1
Query: 256 LQWIASKTATFGERIVAFAAVEXXXXXXXXXXXXWLKKRGLMPGLTFSNELISRDEGLHT 435
L WI S + F R+VAFA VE WLKKRGLMPGLTFSNELISRDEGLH
Sbjct: 160 LDWIQSPMS-FAVRLVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHC 218
Query: 436 DFACLMFQHLVQKPTKECVLHII 504
DFACL++ L ++ E V I+
Sbjct: 219 DFACLLYSLLQKQLPLEKVYQIV 241
Score = 44.8 bits (101), Expect = 7e-05
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = +2
Query: 185 SEERDFLFNAVETLPCVKKKADWVYSGLQARLQHLVK 295
S+E+D LFNA+ET+PC+ KKA W +Q+ + V+
Sbjct: 136 SKEKDRLFNAIETIPCISKKAKWCLDWIQSPMSFAVR 172
>At5g40942.1 68418.m04973 ribonucleoside-diphosphate reductase small
chain, putative / ribonucleotide reductase, putative
similar to ribonucleotide reductase R2 [Nicotiana
tabacum] GI:1044912; contains Pfam profile PF00268:
Ribonucleotide reductase, small chain
Length = 231
Score = 90.6 bits (215), Expect = 1e-18
Identities = 51/78 (65%), Positives = 57/78 (73%)
Frame = +3
Query: 510 AVVIEQEFLTDALPVRLLGMNCELMSQYIEFVADRLLVDLIGGKHYNTKNPFGLS*ILIS 689
AV IE+EF+ DAL L+GMN ELMSQYIEFVADRLL L GK Y NPF LIS
Sbjct: 131 AVEIEREFVCDALSCALVGMNRELMSQYIEFVADRLLAALGCGKVYGVTNPFDWM-ELIS 189
Query: 690 AGKGKTNFFEKKVGDYQK 743
+GKTNFFEK+VG+YQK
Sbjct: 190 L-QGKTNFFEKRVGEYQK 206
Score = 85.8 bits (203), Expect = 3e-17
Identities = 43/68 (63%), Positives = 51/68 (75%)
Frame = +3
Query: 3 FIKHVLAFFAASDGIVNENLVERFSQEVQVTEARCFYGFQIAMENVHSEMYSLLIDTYIR 182
FIKHVLAFFAASDGIV ENL RF +VQ++EAR FYGFQIA+EN+HSE+ LL +
Sbjct: 64 FIKHVLAFFAASDGIVLENLSTRFMSDVQISEARAFYGFQIAIENIHSEI--LLRTKFDE 121
Query: 183 DPKREIFC 206
D + I C
Sbjct: 122 DRLKAIVC 129
>At3g11310.1 68416.m01375 hypothetical protein
Length = 539
Score = 30.3 bits (65), Expect = 1.5
Identities = 27/81 (33%), Positives = 40/81 (49%), Gaps = 9/81 (11%)
Frame = +3
Query: 48 VNENLVERFSQEVQVTEARCFYGFQI----AMENVH--SEMYSLLIDTYIRDPKREIFCL 209
+N LVE+ + V E G + AME V SE+Y L +D ++ RE+F L
Sbjct: 457 INIELVEKITSNALVKEYEYTIGECMKCLNAMEEVEKGSELYMLALDLFMNKECREMFLL 516
Query: 210 MR---LRLFPV*RRKQIGSTV 263
+ LR+ + RR GST+
Sbjct: 517 LETSTLRMSWLLRRLSGGSTM 537
>At1g04860.1 68414.m00482 ubiquitin-specific protease 2 (UBP2)
identical to GI:11993463
Length = 961
Score = 29.1 bits (62), Expect = 3.5
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = -1
Query: 389 SPGIRPRFLSQKMEAKLPEKKIPSTAAKATILS 291
SP +P+ +S+ +AKLP K++P +K + +S
Sbjct: 390 SPPKKPQPVSRAKKAKLPPKRVPKNVSKVSKVS 422
>At5g47150.1 68418.m05812 YDG/SRA domain-containing protein low
similarity to nuclear protein np95 [Mus musculus]
GI:4220590; contains Pfam profile PF02182: YDG/SRA
domain
Length = 328
Score = 28.7 bits (61), Expect = 4.6
Identities = 17/77 (22%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
Frame = +3
Query: 552 VRLLGMNCELMS--QYIEFVADRLLVDLIGGKHYNTKNPFGLS*ILISAGKGKTNFFEKK 725
+R++G++ + M YI+ DR+ ++ + Y + + ++ + G +KK
Sbjct: 193 LRVVGLHSKPMCGIDYIKLGDDRITTSIVASEGYGYNDTYNSGVMVYTGEGGNVINKQKK 252
Query: 726 VGDYQKCRG*LAIALTM 776
D + +G LA+A +M
Sbjct: 253 TEDQKLVKGNLALATSM 269
>At1g49740.1 68414.m05578 expressed protein similar to MAP3K-like
protein kinase GB:CAB16796 GI:4006878 from [Arabidopsis
thaliana]
Length = 359
Score = 28.7 bits (61), Expect = 4.6
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 4/50 (8%)
Frame = +3
Query: 117 FQIAMENVHSEMYSLLIDTYIRDPK--REIFCLMRLR--LFPV*RRKQIG 254
FQ+ +E E+ +++I+ Y++ PK ++F LR +FPV R + G
Sbjct: 158 FQVFLEKNKEEVVTIIIEDYVKSPKGLTKVFDAAGLRKFMFPVSRMPKNG 207
>At5g21930.1 68418.m02545 ATPase E1-E2 type family protein /
haloacid dehalogenase-like hydrolase family protein /
heavy-metal-associated domain-containing protein
contains InterPro accession IPR001757: ATPase, E1-E2
type; contains Pfam profiles PF00403:
Heavy-metal-associated domain, PF00702: haloacid
dehalogenase-like hydrolase
Length = 883
Score = 28.3 bits (60), Expect = 6.1
Identities = 25/78 (32%), Positives = 36/78 (46%), Gaps = 8/78 (10%)
Frame = -2
Query: 700 PFPADIRIHESPKGFLVL*CFPPIR--------STSNLSATNSMY*DISSQFIPSSLTGS 545
P P +H P FLV CFP +R S +NS+ +IS+Q S+ +
Sbjct: 9 PLPPPSSLHIRPSKFLVNRCFPRLRRSRIRRHCSRPFFLVSNSV--EISTQSFESTESSI 66
Query: 544 ASVRNSCSMTTALL*YAG 491
SV++ S T LL +G
Sbjct: 67 ESVKSITSDTPILLDVSG 84
>At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative
strong similarity to gi:4467359
Length = 1116
Score = 28.3 bits (60), Expect = 6.1
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +2
Query: 161 AYRYLHQRSEERDFLFNAVETLP 229
+Y Y HQ S RD+L N + TLP
Sbjct: 53 SYLYKHQHSGVRDYLCNRMYTLP 75
>At1g53140.1 68414.m06017 dynamin family protein low similarity to
dynamin-like protein E [Arabidopsis thaliana]
GI:19423872; contains Pfam profile PF00350: Dynamin
family
Length = 817
Score = 28.3 bits (60), Expect = 6.1
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = -3
Query: 726 LSSQKSLSYPSQRILGFMKVRRGSWCCNAFLQLDQQV 616
LS KSL+ P RIL F++ WC + +L +++
Sbjct: 196 LSMVKSLASPPHRILLFLQQSSVEWCSSLWLDAVREI 232
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,862,426
Number of Sequences: 28952
Number of extensions: 378691
Number of successful extensions: 884
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 859
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 881
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1775300800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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