Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30113
         (712 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putativ...   114   5e-26
At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putativ...   114   5e-26
At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ...   114   5e-26
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp...    36   0.035
At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alp...    35   0.046
At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putat...    32   0.43 
At5g49660.1 68418.m06147 leucine-rich repeat transmembrane prote...    31   0.75 
At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alp...    31   1.00 
At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical...    29   4.0  
At3g12540.1 68416.m01560 expressed protein contains Pfam profile...    27   9.3  

>At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putative /
           3-methyl-2-oxobutanoate dehydrogenase, putative /
           branched-chain alpha-keto acid dehydrogenase E1 alpha
           subunit, putative similar to branched-chain alpha-keto
           acid dehydrogenase E1-alpha subunit [Gallus gallus]
           GI:12964598; contains Pfam profile PF00676:
           Dehydrogenase E1 component
          Length = 401

 Score =  114 bits (275), Expect = 5e-26
 Identities = 57/118 (48%), Positives = 70/118 (59%)
 Frame = +3

Query: 258 YESQRQGRISFYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTGLVNQCY 437
           YE+QRQGR+SFY T  GEE I+I SA+AL+P+D++F QYRE GV L+RG T+    NQC+
Sbjct: 77  YEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCF 136

Query: 438 GNCEDPGKRRQMPVHYGSKHHNMVQYLVH*EHXCPRLLEPLMPTKEFPYNDRCVICYF 611
           GN  D GK RQMPVHYGS   N            P  +      K     D C + YF
Sbjct: 137 GNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLK-MDKKDACAVTYF 193



 Score = 60.1 bits (139), Expect = 1e-09
 Identities = 23/70 (32%), Positives = 43/70 (61%)
 Frame = +1

Query: 49  KIAEFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTM 228
           ++ +FPG +  +  E++F +E+  E +P YRV+D+NGQ+I  ++   + +   + +Y  M
Sbjct: 7   QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 66

Query: 229 VQLSHMDKIY 258
           V L  MD I+
Sbjct: 67  VTLQIMDNIF 76



 Score = 39.5 bits (88), Expect = 0.002
 Identities = 22/66 (33%), Positives = 29/66 (43%)
 Frame = +2

Query: 509 TISSPLGTXMPQAVGAAYAYKXXXXXXXXXXXXXSVTGAASEGDAHSAFNFAGYVRLSCY 688
           T+S+ + T +P AVGAAY+ K                G  SEGD H+A N A  +     
Sbjct: 161 TVSATIATQLPNAVGAAYSLKMDKKDACAVTYFGD--GGTSEGDFHAALNIAAVMEAPVL 218

Query: 689 *CCAGN 706
             C  N
Sbjct: 219 FICRNN 224


>At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putative /
           3-methyl-2-oxobutanoate dehydrogenase, putative /
           branched-chain alpha-keto acid dehydrogenase E1 alpha
           subunit, putative similar to branched-chain alpha-keto
           acid dehydrogenase E1-alpha subunit [Gallus gallus]
           GI:12964598; contains Pfam profile PF00676:
           Dehydrogenase E1 component
          Length = 472

 Score =  114 bits (275), Expect = 5e-26
 Identities = 57/118 (48%), Positives = 70/118 (59%)
 Frame = +3

Query: 258 YESQRQGRISFYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTGLVNQCY 437
           YE+QRQGR+SFY T  GEE I+I SA+AL+P+D++F QYRE GV L+RG T+    NQC+
Sbjct: 148 YEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCF 207

Query: 438 GNCEDPGKRRQMPVHYGSKHHNMVQYLVH*EHXCPRLLEPLMPTKEFPYNDRCVICYF 611
           GN  D GK RQMPVHYGS   N            P  +      K     D C + YF
Sbjct: 208 GNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLK-MDKKDACAVTYF 264



 Score = 61.3 bits (142), Expect = 6e-10
 Identities = 28/85 (32%), Positives = 50/85 (58%)
 Frame = +1

Query: 4   LSTRTRPETAQNGGGKIAEFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEE 183
           LS+  + E A N   ++ +FPG +  +  E++F +E+  E +P YRV+D+NGQ+I  ++ 
Sbjct: 65  LSSFVQHEYANNH--QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQF 122

Query: 184 PNLDKATLINMYKTMVQLSHMDKIY 258
             + +   + +Y  MV L  MD I+
Sbjct: 123 VQVSEEVAVKIYSDMVTLQIMDNIF 147



 Score = 39.5 bits (88), Expect = 0.002
 Identities = 22/66 (33%), Positives = 29/66 (43%)
 Frame = +2

Query: 509 TISSPLGTXMPQAVGAAYAYKXXXXXXXXXXXXXSVTGAASEGDAHSAFNFAGYVRLSCY 688
           T+S+ + T +P AVGAAY+ K                G  SEGD H+A N A  +     
Sbjct: 232 TVSATIATQLPNAVGAAYSLKMDKKDACAVTYFGD--GGTSEGDFHAALNIAAVMEAPVL 289

Query: 689 *CCAGN 706
             C  N
Sbjct: 290 FICRNN 295


>At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative /
           3-methyl-2-oxobutanoate dehydrogenase, putative /
           branched-chain alpha-keto acid dehydrogenase E1 alpha
           subunit, putative similar to branched-chain alpha-keto
           acid dehydrogenase E1-alpha subunit [Gallus gallus]
           GI:12964598; contains Pfam profile PF00676:
           Dehydrogenase E1 component
          Length = 472

 Score =  114 bits (275), Expect = 5e-26
 Identities = 52/82 (63%), Positives = 62/82 (75%)
 Frame = +3

Query: 258 YESQRQGRISFYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTGLVNQCY 437
           YE+QRQGRISFY+T+ GEE I+I SA+ALSP D+V  QYRE GV L+RG T+    NQC+
Sbjct: 148 YEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCF 207

Query: 438 GNCEDPGKRRQMPVHYGSKHHN 503
           GN  D GK RQMP+HYGS   N
Sbjct: 208 GNKADYGKGRQMPIHYGSNRLN 229



 Score = 58.8 bits (136), Expect = 3e-09
 Identities = 27/67 (40%), Positives = 40/67 (59%)
 Frame = +1

Query: 58  EFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQL 237
           +FPG +  Y SEMKF  E+S   IP YRV+D +G+II  ++   + +   + MY+ M  L
Sbjct: 81  DFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATL 140

Query: 238 SHMDKIY 258
             MD I+
Sbjct: 141 QVMDHIF 147



 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 23/66 (34%), Positives = 28/66 (42%)
 Frame = +2

Query: 509 TISSPLGTXMPQAVGAAYAYKXXXXXXXXXXXXXSVTGAASEGDAHSAFNFAGYVRLSCY 688
           TISSP+ T +PQA G  Y+ K                G  SEGD H+  NFA  +     
Sbjct: 232 TISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGD--GGTSEGDFHAGLNFAAVMEAPVV 289

Query: 689 *CCAGN 706
             C  N
Sbjct: 290 FICRNN 295


>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
           subunit, mitochondrial (PDHE1-A) identical to SP|P52901
           Pyruvate dehydrogenase E1 component alpha subunit,
           mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
           {Arabidopsis thaliana}
          Length = 389

 Score = 35.5 bits (78), Expect = 0.035
 Identities = 17/63 (26%), Positives = 34/63 (53%)
 Frame = +3

Query: 306 GEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTGLVNQCYGNCEDPGKRRQMPVHY 485
           G+E + IG  +A++ KD + + YR+  +FL RG ++  + ++  G      K +   +H+
Sbjct: 94  GQEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHF 153

Query: 486 GSK 494
             K
Sbjct: 154 YKK 156


>At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alpha
           subunit, chloroplast identical to pyruvate dehydrogenase
           E1 alpha subunit GB:AAB86803 GI:2454182 from
           [Arabidopsis thaliana]; identical to cDNA pyruvate
           dehydrogenase E1 alpha subunit mRNA, nuclear gene
           encoding plastid protein GI:2454181
          Length = 428

 Score = 35.1 bits (77), Expect = 0.046
 Identities = 18/68 (26%), Positives = 34/68 (50%)
 Frame = +3

Query: 306 GEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTGLVNQCYGNCEDPGKRRQMPVHY 485
           G+E +  G    L+  D V S YR+    L +G++   ++++ +G      + +   +H 
Sbjct: 119 GQEAVSTGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHM 178

Query: 486 GSKHHNMV 509
            SK HNM+
Sbjct: 179 FSKEHNML 186


>At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putative
           / serine hydroxymethyltransferase, putative /
           serine/threonine aldolase, putative similar to serine
           hydroxymethyltransferase [Chlamydomonas reinhardtii]
           GI:17066746; contains Pfam profile PF00464: serine
           hydroxymethyltransferase
          Length = 470

 Score = 31.9 bits (69), Expect = 0.43
 Identities = 14/42 (33%), Positives = 27/42 (64%)
 Frame = +3

Query: 315 GIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTGLVNQCYG 440
           G+ IG+  A++ + LV   + ++G FL+R +T+T  + + YG
Sbjct: 387 GVRIGTP-AMTSRGLVEKDFEKIGEFLHRAVTITLDIQEQYG 427


>At5g49660.1 68418.m06147 leucine-rich repeat transmembrane protein
           kinase, putative contains leucine rich repeat (LRR)
           domains, Pfam:PF00560; contains protein kinase domain,
           Pfam:PF00069
          Length = 966

 Score = 31.1 bits (67), Expect = 0.75
 Identities = 18/67 (26%), Positives = 35/67 (52%)
 Frame = +1

Query: 46  GKIAEFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKT 225
           G + +F   ++  V +M + + T   P+ I+ + D   + ++ NE P LD  TL +    
Sbjct: 136 GTLPDFSQMKSLRVIDMSWNHFTGSFPLSIFNLTDL--EYLNFNENPELDLWTLPDSVSK 193

Query: 226 MVQLSHM 246
           + +L+HM
Sbjct: 194 LTKLTHM 200


>At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alpha
           subunit, mitochondrial, putative similar to SP|P52901
           Pyruvate dehydrogenase E1 component alpha subunit,
           mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
           {Arabidopsis thaliana}; contains Pfam profile PF00676:
           Dehydrogenase E1 component
          Length = 393

 Score = 30.7 bits (66), Expect = 1.00
 Identities = 12/45 (26%), Positives = 25/45 (55%)
 Frame = +3

Query: 306 GEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTGLVNQCYG 440
           G+E + +G  +A++ KD + + YR+   F+ RG  +    ++  G
Sbjct: 98  GQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSELMG 142


>At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical to
           DNA Helicase [Arabidopsis thaliana] GI:11121449
          Length = 1188

 Score = 28.7 bits (61), Expect = 4.0
 Identities = 15/47 (31%), Positives = 26/47 (55%)
 Frame = -2

Query: 291 KMKCDLVFVIHIDFIHMAQLYHGFVHVNQRSLIQVRFFIFVDDLSVI 151
           +M C+ V     +F H A  YHG +   QR+ IQ ++    D++++I
Sbjct: 683 RMDCEKVSERLQEFGHKAAFYHGSMEPEQRAFIQTQW--SKDEINII 727


>At3g12540.1 68416.m01560 expressed protein contains Pfam profile
           PF04784: Protein of unknown function, DUF547
          Length = 503

 Score = 27.5 bits (58), Expect = 9.3
 Identities = 11/28 (39%), Positives = 19/28 (67%)
 Frame = +1

Query: 202 TLINMYKTMVQLSHMDKIYMNHKDKVAF 285
           +L+N+     +LS +D  ++NHK K+AF
Sbjct: 275 SLVNLRVLKEKLSKVDLSFLNHKKKMAF 302


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,677,457
Number of Sequences: 28952
Number of extensions: 330732
Number of successful extensions: 790
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 765
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 787
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)