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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30106
         (708 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   142   6e-35
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   138   5e-34
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    91   1e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    62   8e-11
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr...    28   1.5  
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po...    27   2.6  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   6.1  
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    25   8.0  
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos...    25   8.0  
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom...    25   8.0  
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce...    25   8.0  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  142 bits (343), Expect = 6e-35
 Identities = 60/87 (68%), Positives = 73/87 (83%)
 Frame = +3

Query: 225 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 404
           F  F      GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49  FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108

Query: 405 TIGKEIVDLVLDRIRKLADQCTGLQGF 485
           T+GKE+VD V D+IR++AD C+GLQGF
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGF 135



 Score = 94.3 bits (224), Expect = 2e-20
 Identities = 41/63 (65%), Positives = 45/63 (71%)
 Frame = +1

Query: 82  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 261
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG   
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 262 TYP 270
             P
Sbjct: 61  YVP 63



 Score = 33.1 bits (72), Expect = 0.040
 Identities = 24/49 (48%), Positives = 32/49 (65%)
 Frame = +1

Query: 511 TGSGVTSLLMERLSVELTARKSKLGVRHLTPRPQGFQLAVVRGPKNSIL 657
           TGSG  +LL+ERL++E T +KSKL    + P PQ    +VV  P NS+L
Sbjct: 145 TGSGFGALLLERLAMEYT-KKSKLQF-SVYPAPQ-VSTSVVE-PYNSVL 189


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  138 bits (335), Expect = 5e-34
 Identities = 57/87 (65%), Positives = 73/87 (83%)
 Frame = +3

Query: 225 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 404
           F  F      GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53  FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112

Query: 405 TIGKEIVDLVLDRIRKLADQCTGLQGF 485
           T+GKE++D VL+RIR++AD C+GLQGF
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGF 139



 Score = 88.2 bits (209), Expect = 1e-18
 Identities = 45/68 (66%), Positives = 47/68 (69%), Gaps = 5/68 (7%)
 Frame = +1

Query: 82  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 246
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 247 TGAASTYP 270
           TG     P
Sbjct: 60  TGQGKFVP 67



 Score = 31.5 bits (68), Expect = 0.12
 Identities = 26/61 (42%), Positives = 36/61 (59%)
 Frame = +1

Query: 511 TGSGVTSLLMERLSVELTARKSKLGVRHLTPRPQGFQLAVVRGPKNSILNQPTKNPLSNS 690
           TGSG+ +LL+ERL++E   +KS L    + P PQ    +VV  P NS+L   T   L NS
Sbjct: 149 TGSGLGALLLERLNMEY-GKKSNLQF-SVYPAPQ-VSTSVVE-PYNSVLT--THATLDNS 202

Query: 691 E 693
           +
Sbjct: 203 D 203


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 91.1 bits (216), Expect = 1e-19
 Identities = 40/77 (51%), Positives = 54/77 (70%)
 Frame = +3

Query: 255 GKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLV 434
           GK+VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D V
Sbjct: 57  GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116

Query: 435 LDRIRKLADQCTGLQGF 485
           LD +R+ A+ C  LQGF
Sbjct: 117 LDVVRREAEACDALQGF 133



 Score = 36.3 bits (80), Expect = 0.004
 Identities = 19/63 (30%), Positives = 26/63 (41%)
 Frame = +1

Query: 82  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 261
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E     
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 262 TYP 270
             P
Sbjct: 59  YVP 61


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 62.1 bits (144), Expect = 8e-11
 Identities = 32/86 (37%), Positives = 53/86 (61%), Gaps = 2/86 (2%)
 Frame = +3

Query: 234 FLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 407
           F Q D + +++PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+
Sbjct: 52  FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109

Query: 408 IGKEIVDLVLDRIRKLADQCTGLQGF 485
             + I + ++D I + AD    L+GF
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGF 135



 Score = 48.8 bits (111), Expect = 8e-07
 Identities = 24/55 (43%), Positives = 33/55 (60%)
 Frame = +1

Query: 85  RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 249
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55


>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1024

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
 Frame = -2

Query: 317 ADLINNSRFKIDEDS--TGYVLAAPVSLKKVLKESSP 213
           AD+IN SR+ ID  S  T +    P+SL    KE  P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491


>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 399

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
 Frame = -2

Query: 350 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIW 183
           F    + +  S  +++N+ +  KID    GY      S K  + E +  P  +  G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +3

Query: 180 WPDAHRQDHRGWRRFFQHFLQR 245
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 9/17 (52%), Positives = 14/17 (82%)
 Frame = +1

Query: 646 NSILNQPTKNPLSNSEL 696
           N +LN+P+ +PLS+S L
Sbjct: 926 NRVLNEPSSSPLSSSSL 942


>SPBC119.07 |ppk19||serine/threonine protein kinase
           Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1706

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 13/40 (32%), Positives = 19/40 (47%)
 Frame = +3

Query: 183 PDAHRQDHRGWRRFFQHFLQRDRSGKHVPRAVFVDLEPTV 302
           P+ H +   G   F + F  +DR G  V   VFV+  P +
Sbjct: 22  PEYHNERSLGDSHFLRTFRMQDRKGYDVLIKVFVNKLPEI 61


>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 534

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = +2

Query: 71  LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 193
           L+S        +L+ P SR++ P  S +  STASSL    P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210


>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1067

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 12/37 (32%), Positives = 22/37 (59%)
 Frame = -2

Query: 284 DEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIWPSG 174
           ++D T Y L  P+   + L +++PP +V+ +G  P G
Sbjct: 834 EKDLTKY-LGPPIYTSQRLYDTTPPGVVMGLGWTPMG 869


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,151,542
Number of Sequences: 5004
Number of extensions: 68943
Number of successful extensions: 192
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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