BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30106 (708 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 142 6e-35 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 138 5e-34 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 91 1e-19 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 62 8e-11 SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.5 SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 27 2.6 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.1 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 25 8.0 SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 8.0 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.0 SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 25 8.0 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 142 bits (343), Expect = 6e-35 Identities = 60/87 (68%), Positives = 73/87 (83%) Frame = +3 Query: 225 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 404 F F GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108 Query: 405 TIGKEIVDLVLDRIRKLADQCTGLQGF 485 T+GKE+VD V D+IR++AD C+GLQGF Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGF 135 Score = 94.3 bits (224), Expect = 2e-20 Identities = 41/63 (65%), Positives = 45/63 (71%) Frame = +1 Query: 82 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 261 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 262 TYP 270 P Sbjct: 61 YVP 63 Score = 33.1 bits (72), Expect = 0.040 Identities = 24/49 (48%), Positives = 32/49 (65%) Frame = +1 Query: 511 TGSGVTSLLMERLSVELTARKSKLGVRHLTPRPQGFQLAVVRGPKNSIL 657 TGSG +LL+ERL++E T +KSKL + P PQ +VV P NS+L Sbjct: 145 TGSGFGALLLERLAMEYT-KKSKLQF-SVYPAPQ-VSTSVVE-PYNSVL 189 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 138 bits (335), Expect = 5e-34 Identities = 57/87 (65%), Positives = 73/87 (83%) Frame = +3 Query: 225 FQHFLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 404 F F GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112 Query: 405 TIGKEIVDLVLDRIRKLADQCTGLQGF 485 T+GKE++D VL+RIR++AD C+GLQGF Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGF 139 Score = 88.2 bits (209), Expect = 1e-18 Identities = 45/68 (66%), Positives = 47/68 (69%), Gaps = 5/68 (7%) Frame = +1 Query: 82 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 246 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 247 TGAASTYP 270 TG P Sbjct: 60 TGQGKFVP 67 Score = 31.5 bits (68), Expect = 0.12 Identities = 26/61 (42%), Positives = 36/61 (59%) Frame = +1 Query: 511 TGSGVTSLLMERLSVELTARKSKLGVRHLTPRPQGFQLAVVRGPKNSILNQPTKNPLSNS 690 TGSG+ +LL+ERL++E +KS L + P PQ +VV P NS+L T L NS Sbjct: 149 TGSGLGALLLERLNMEY-GKKSNLQF-SVYPAPQ-VSTSVVE-PYNSVLT--THATLDNS 202 Query: 691 E 693 + Sbjct: 203 D 203 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 91.1 bits (216), Expect = 1e-19 Identities = 40/77 (51%), Positives = 54/77 (70%) Frame = +3 Query: 255 GKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLV 434 GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D V Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116 Query: 435 LDRIRKLADQCTGLQGF 485 LD +R+ A+ C LQGF Sbjct: 117 LDVVRREAEACDALQGF 133 Score = 36.3 bits (80), Expect = 0.004 Identities = 19/63 (30%), Positives = 26/63 (41%) Frame = +1 Query: 82 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAAS 261 MRE + + GQ G Q+G A W EHG+ G T + N +F+E Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 262 TYP 270 P Sbjct: 59 YVP 61 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 62.1 bits (144), Expect = 8e-11 Identities = 32/86 (37%), Positives = 53/86 (61%), Gaps = 2/86 (2%) Frame = +3 Query: 234 FLQRDRSGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 407 F Q D + +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109 Query: 408 IGKEIVDLVLDRIRKLADQCTGLQGF 485 + I + ++D I + AD L+GF Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGF 135 Score = 48.8 bits (111), Expect = 8e-07 Identities = 24/55 (43%), Positives = 33/55 (60%) Frame = +1 Query: 85 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 249 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55 >SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 1024 Score = 27.9 bits (59), Expect = 1.5 Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%) Frame = -2 Query: 317 ADLINNSRFKIDEDS--TGYVLAAPVSLKKVLKESSP 213 AD+IN SR+ ID S T + P+SL KE P Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491 >SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 399 Score = 27.1 bits (57), Expect = 2.6 Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%) Frame = -2 Query: 350 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIW 183 F + + S +++N+ + KID GY S K + E + P + G+W Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 6.1 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +3 Query: 180 WPDAHRQDHRGWRRFFQHFLQR 245 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 25.4 bits (53), Expect = 8.0 Identities = 9/17 (52%), Positives = 14/17 (82%) Frame = +1 Query: 646 NSILNQPTKNPLSNSEL 696 N +LN+P+ +PLS+S L Sbjct: 926 NRVLNEPSSSPLSSSSL 942 >SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizosaccharomyces pombe|chr 2|||Manual Length = 1706 Score = 25.4 bits (53), Expect = 8.0 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = +3 Query: 183 PDAHRQDHRGWRRFFQHFLQRDRSGKHVPRAVFVDLEPTV 302 P+ H + G F + F +DR G V VFV+ P + Sbjct: 22 PEYHNERSLGDSHFLRTFRMQDRKGYDVLIKVFVNKLPEI 61 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 8.0 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +2 Query: 71 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 193 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 >SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1067 Score = 25.4 bits (53), Expect = 8.0 Identities = 12/37 (32%), Positives = 22/37 (59%) Frame = -2 Query: 284 DEDSTGYVLAAPVSLKKVLKESSPPPMVLSVGIWPSG 174 ++D T Y L P+ + L +++PP +V+ +G P G Sbjct: 834 EKDLTKY-LGPPIYTSQRLYDTTPPGVVMGLGWTPMG 869 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,151,542 Number of Sequences: 5004 Number of extensions: 68943 Number of successful extensions: 192 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 174 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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