Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30100
(637 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 152 4e-38
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 78 1e-15
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 72 6e-14
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 59 6e-10
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 54 2e-08
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 52 9e-08
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 49 6e-07
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 46 5e-06
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 33 0.046
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 30 0.32
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 152 bits (369), Expect = 4e-38
Identities = 69/82 (84%), Positives = 74/82 (90%)
Frame = +2
Query: 2 RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
R PEALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQK
Sbjct: 256 RAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQK 315
Query: 182 EITALAPSTMKIKIIAPPERKY 247
EI ALAPS+MK+KI+APPERKY
Sbjct: 316 EIQALAPSSMKVKIVAPPERKY 337
Score = 74.1 bits (174), Expect = 2e-14
Identities = 33/35 (94%), Positives = 34/35 (97%)
Frame = +1
Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
IGGSILASLSTFQQMWISKQEYDESGP IV+RKCF
Sbjct: 341 IGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 77.8 bits (183), Expect = 1e-15
Identities = 34/80 (42%), Positives = 50/80 (62%)
Frame = +2
Query: 8 PEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEI 187
PE LF P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +R E+
Sbjct: 263 PEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISEL 322
Query: 188 TALAPSTMKIKIIAPPERKY 247
A++ ++KI A PER +
Sbjct: 323 RAISGKKNQVKIYASPERMH 342
Score = 35.9 bits (79), Expect = 0.005
Identities = 16/35 (45%), Positives = 28/35 (80%)
Frame = +1
Query: 256 IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 360
+GGSILASLSTF+++ I+ +EY ++ +++ R+ F
Sbjct: 346 LGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 72.1 bits (169), Expect = 6e-14
Identities = 33/64 (51%), Positives = 43/64 (67%)
Frame = +2
Query: 5 CPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKE 184
CPE LFQP +G E G+ E +++I DVDIRK LY VLSGG++MY G+ R++KE
Sbjct: 257 CPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKE 316
Query: 185 ITAL 196
I L
Sbjct: 317 IKQL 320
Score = 28.7 bits (61), Expect = 0.74
Identities = 12/27 (44%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
Frame = +1
Query: 256 IGGSILASL-STFQQMWISKQEYDESG 333
IGG++LA + + MW+SK E++E G
Sbjct: 352 IGGAVLADIMAQNDHMWVSKAEWEEYG 378
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 58.8 bits (136), Expect = 6e-10
Identities = 29/75 (38%), Positives = 43/75 (57%), Gaps = 9/75 (12%)
Frame = +2
Query: 2 RCPEALFQPSFLGMEAC---------GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMY 154
R E LF PSF + G+HE Y SI+ CD ++R L N V++GGT++
Sbjct: 297 RISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLI 356
Query: 155 PGIADRMQKEITALA 199
PG+++R+Q E+ LA
Sbjct: 357 PGLSERLQAEVQRLA 371
Score = 46.0 bits (104), Expect = 5e-06
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = +1
Query: 259 GGSILASLSTFQQMWISKQEYDESG 333
GGSILASL FQ +W+SKQEYDE G
Sbjct: 394 GGSILASLDNFQHLWVSKQEYDEVG 418
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 53.6 bits (123), Expect = 2e-08
Identities = 25/77 (32%), Positives = 39/77 (50%)
Frame = +2
Query: 8 PEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEI 187
PE LF PS + + GI E S+ +I+ L N V GG +PG R+ E+
Sbjct: 284 PELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSEL 343
Query: 188 TALAPSTMKIKIIAPPE 238
+LAP+ ++K+ P +
Sbjct: 344 RSLAPANWEVKVFEPSD 360
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 51.6 bits (118), Expect = 9e-08
Identities = 21/74 (28%), Positives = 44/74 (59%)
Frame = +2
Query: 2 RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 181
R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R+++
Sbjct: 597 RVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETRIKR 656
Query: 182 EITALAPSTMKIKI 223
E+T++ P I +
Sbjct: 657 ELTSIMPVGSSINV 670
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 48.8 bits (111), Expect = 6e-07
Identities = 24/59 (40%), Positives = 37/59 (62%)
Frame = +2
Query: 53 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIA 229
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHA 383
Score = 46.8 bits (106), Expect = 3e-06
Identities = 19/37 (51%), Positives = 29/37 (78%), Gaps = 3/37 (8%)
Frame = +1
Query: 256 IGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 357
+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 396 LGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 46.0 bits (104), Expect = 5e-06
Identities = 20/61 (32%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Frame = +2
Query: 8 PEALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKE 184
PE F P + + E N + +D+RK LY N VLSGG+T++ +R+Q++
Sbjct: 290 PEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRD 349
Query: 185 I 187
+
Sbjct: 350 L 350
Score = 27.5 bits (58), Expect = 1.7
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 259 GGSILASLSTFQQMWISKQEYDESGPSIVHR 351
GGS+LA F +K +Y+E G SI R
Sbjct: 389 GGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 32.7 bits (71), Expect = 0.046
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +2
Query: 107 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP 235
K+LY++ ++ GG +PG A +++ I + + I +I PP
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPP 569
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 29.9 bits (64), Expect = 0.32
Identities = 14/41 (34%), Positives = 25/41 (60%), Gaps = 3/41 (7%)
Frame = +1
Query: 241 EVLRMIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 354
E L +GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 482 EELAFLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,560,971
Number of Sequences: 5004
Number of extensions: 47780
Number of successful extensions: 132
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -