Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30093
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 116 5e-27
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 116 5e-27
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 116 5e-27
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 31 0.23
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 30 0.40
SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces... 29 0.54
SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces pomb... 29 0.94
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 28 1.2
SPAC25G10.08 |||translation initiation factor eIF3b |Schizosacch... 28 1.6
SPAC31G5.15 |||phosphatidylserine decarboxylase |Schizosaccharom... 27 2.9
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 6.6
SPAC1486.09 |||ribosome biogenesis protein Nob1 |Schizosaccharom... 26 6.6
SPAP14E8.03 |bos1||SNARE Bos1|Schizosaccharomyces pombe|chr 1|||... 25 8.7
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 116 bits (278), Expect = 5e-27
Identities = 53/83 (63%), Positives = 69/83 (83%)
Frame = +1
Query: 19 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 198
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 199 VHFRDPYKFKTRKELFIAPKPLH 267
V FR+PY ++T E F+A + ++
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMY 82
Score = 81.0 bits (191), Expect = 2e-16
Identities = 35/46 (76%), Positives = 38/46 (82%)
Frame = +2
Query: 263 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R
Sbjct: 82 YTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGR 127
Score = 81.0 bits (191), Expect = 2e-16
Identities = 37/67 (55%), Positives = 51/67 (76%)
Frame = +3
Query: 402 ASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPIFESWKGHTT 581
+SGN+ ++GH+ D +TRVKLPSGAKKV+PS + +VGIVAGGGRIDKP+ ++ +
Sbjct: 128 SSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAF-H 186
Query: 582 SNKVKRN 602
+VKRN
Sbjct: 187 KYRVKRN 193
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 116 bits (278), Expect = 5e-27
Identities = 53/83 (63%), Positives = 69/83 (83%)
Frame = +1
Query: 19 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 198
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 199 VHFRDPYKFKTRKELFIAPKPLH 267
V FR+PY ++T E F+A + ++
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMY 82
Score = 81.0 bits (191), Expect = 2e-16
Identities = 35/46 (76%), Positives = 38/46 (82%)
Frame = +2
Query: 263 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R
Sbjct: 82 YTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGR 127
Score = 81.0 bits (191), Expect = 2e-16
Identities = 37/67 (55%), Positives = 51/67 (76%)
Frame = +3
Query: 402 ASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPIFESWKGHTT 581
+SGN+ ++GH+ D +TRVKLPSGAKKV+PS + +VGIVAGGGRIDKP+ ++ +
Sbjct: 128 SSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAF-H 186
Query: 582 SNKVKRN 602
+VKRN
Sbjct: 187 KYRVKRN 193
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 116 bits (278), Expect = 5e-27
Identities = 53/83 (63%), Positives = 69/83 (83%)
Frame = +1
Query: 19 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 198
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 199 VHFRDPYKFKTRKELFIAPKPLH 267
V FR+PY ++T E F+A + ++
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMY 82
Score = 81.0 bits (191), Expect = 2e-16
Identities = 35/46 (76%), Positives = 38/46 (82%)
Frame = +2
Query: 263 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R
Sbjct: 82 YTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGR 127
Score = 81.0 bits (191), Expect = 2e-16
Identities = 37/67 (55%), Positives = 51/67 (76%)
Frame = +3
Query: 402 ASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPIFESWKGHTT 581
+SGN+ ++GH+ D +TRVKLPSGAKKV+PS + +VGIVAGGGRIDKP+ ++ +
Sbjct: 128 SSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAF-H 186
Query: 582 SNKVKRN 602
+VKRN
Sbjct: 187 KYRVKRN 193
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 30.7 bits (66), Expect = 0.23
Identities = 20/66 (30%), Positives = 29/66 (43%)
Frame = +1
Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
T P+ L L SV P R C+ +A+ + E+D L+ K I Q+ R
Sbjct: 168 THPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLR 227
Query: 592 SNVTGW 609
N+T W
Sbjct: 228 DNLTLW 233
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 29.9 bits (64), Expect = 0.40
Identities = 19/66 (28%), Positives = 27/66 (40%)
Frame = +1
Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
T P+ L L SV P CH + + + E+D L+ K I Q+ R
Sbjct: 167 TDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKDSTLIMQLLR 226
Query: 592 SNVTGW 609
N+T W
Sbjct: 227 DNLTLW 232
>SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 897
Score = 29.5 bits (63), Expect = 0.54
Identities = 15/49 (30%), Positives = 20/49 (40%)
Frame = -3
Query: 529 PATIPTMLCCLDGRTFLAPDGSFTLVRLASGLCPITVAKFPEATCQTTS 383
PAT+P F P F +L SGL V+ +P+ Q S
Sbjct: 246 PATVPITQAASTANAFQQPSNQFQTQKLPSGLDTRPVSSYPDELTQLES 294
>SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 281
Score = 28.7 bits (61), Expect = 0.94
Identities = 15/53 (28%), Positives = 25/53 (47%)
Frame = +3
Query: 342 RVPLCAILKRKWVIEVVWHVASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSR 500
++PL + + W E W G A ++G + +R K P GA + PS+
Sbjct: 119 KLPLLTVNLKSWKFEPPWPAIFGFVAVILGLS--YRRMDTKYPLGAAPLRPSQ 169
>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 564
Score = 28.3 bits (60), Expect = 1.2
Identities = 12/46 (26%), Positives = 22/46 (47%)
Frame = -2
Query: 560 FKNRFVNTSTSSNNTDHALLP*WQNLLGSRR*LYSCTLSIRIVSNH 423
F ++N S ++ L+P +Q ++ S Y TL + + NH
Sbjct: 276 FLQLYINFSFTATGAGFTLIPTYQEVMASNCSKYDSTLMLTFIENH 321
>SPAC25G10.08 |||translation initiation factor eIF3b
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 725
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -2
Query: 629 NTTLHYGQPVTFDLVTCGMALPAFKNRFVNTSTSSNNTDHAL 504
NT + +TFD TC A K RF+ +T ++T + L
Sbjct: 500 NTPSTFRHIITFDKKTCNSLFMAPKGRFMVAATLGSSTQYDL 541
>SPAC31G5.15 |||phosphatidylserine decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 980
Score = 27.1 bits (57), Expect = 2.9
Identities = 16/47 (34%), Positives = 21/47 (44%), Gaps = 2/47 (4%)
Frame = +3
Query: 351 LCAILKRKWVIEVVWHVASGNFATVIGHNPD--AKRTRVKLPSGAKK 485
LC K KW ++V G A ++ NPD K + PSG K
Sbjct: 111 LCLYRKSKWKKQIVGEAYIGIQALLLSTNPDETCKYPVISPPSGRNK 157
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 387 PLSPIFSSRLHTMVPSGIAPTGITFPTSRVAFF 289
P++P+F SR H P G+ T FP F+
Sbjct: 11 PINPVFPSRAHPSPPLGL--TSNLFPIQTNKFY 41
>SPAC1486.09 |||ribosome biogenesis protein Nob1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +1
Query: 199 VHFRDPYKFKTRKELFIAPKPLH 267
VH + +++KTR + PKP+H
Sbjct: 294 VHLKKNFEWKTRGTKYSLPKPVH 316
>SPAP14E8.03 |bos1||SNARE Bos1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 235
Score = 25.4 bits (53), Expect = 8.7
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = -2
Query: 638 SMANTTLHYGQPVTFDLVTCGMALPAFKNRFVNTSTSSNNTDHAL 504
S + L G+ + DLV G L A K + +N + + T H L
Sbjct: 158 SQVDEFLERGRMILGDLVEQGSVLKATKTKVLNAANTLGITRHTL 202
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,358,734
Number of Sequences: 5004
Number of extensions: 73870
Number of successful extensions: 193
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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