SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30093
         (750 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso...   126   2e-29
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)             126   2e-29
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso...   118   3e-27
At1g34760.1 68414.m04323 14-3-3 protein GF14 omicron (GRF11) ide...    36   0.038
At1g26480.1 68414.m03229 14-3-3 protein GF14 iota (GRF12) identi...    36   0.038
At2g42590.1 68415.m05270 14-3-3 protein GF14 mu (GRF9) identical...    34   0.088
At4g09000.1 68417.m01487 14-3-3-like protein GF14 chi /  general...    30   1.4  
At1g78300.1 68414.m09125 14-3-3 protein GF14 omega (GRF2) identi...    30   1.4  
At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identica...    30   1.4  
At1g13540.1 68414.m01587 expressed protein                             30   1.9  
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim...    29   2.5  
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim...    29   2.5  
At5g38480.1 68418.m04651 14-3-3 protein GF14 psi (GRF3) (RCI1) i...    29   3.3  
At3g02520.1 68416.m00240 14-3-3 protein GF14 nu (GRF7) identical...    29   3.3  
At1g18900.2 68414.m02353 pentatricopeptide (PPR) repeat-containi...    29   3.3  
At1g18900.1 68414.m02352 pentatricopeptide (PPR) repeat-containi...    29   3.3  
At3g30520.1 68416.m03863 hypothetical protein                          28   5.8  
At1g53170.1 68414.m06025 ethylene-responsive element-binding fac...    28   5.8  
At4g24900.1 68417.m03564 expressed protein                             28   7.6  

>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
           protein L8, cytosolic, tomato, PIR1:R5TOL8
          Length = 258

 Score =  126 bits (304), Expect = 2e-29
 Identities = 58/83 (69%), Positives = 69/83 (83%)
 Frame = +1

Query: 19  MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 198
           MGRVIRAQRKGAGSVF SHT  RKG  K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA 
Sbjct: 1   MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60

Query: 199 VHFRDPYKFKTRKELFIAPKPLH 267
           V FR P++FK +KELF+A + ++
Sbjct: 61  VAFRHPFRFKKQKELFVAAEGMY 83



 Score = 75.4 bits (177), Expect = 4e-14
 Identities = 30/46 (65%), Positives = 39/46 (84%)
 Frame = +2

Query: 263 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
           YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E  +GDRG  AR
Sbjct: 83  YTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHVGDRGVFAR 128



 Score = 75.4 bits (177), Expect = 4e-14
 Identities = 47/105 (44%), Positives = 66/105 (62%), Gaps = 1/105 (0%)
 Frame = +3

Query: 402 ASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPIFESWKGHTT 581
           ASG++A VI HNPD   +R+KLPSG+KK++PS  ++M+G VAGGGR +KP+ ++   +  
Sbjct: 129 ASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAY-H 187

Query: 582 SNKVKRN-WLAIM*RGVGH*NLLSIPSRVVVTNHTL*GKASTGQR 713
             +VKRN W  +  RGV   N +  P      NH   G AST +R
Sbjct: 188 KYRVKRNCWPKV--RGVAM-NPVEHPHG--GGNHQHIGHASTVRR 227


>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 
          Length = 258

 Score =  126 bits (304), Expect = 2e-29
 Identities = 58/83 (69%), Positives = 69/83 (83%)
 Frame = +1

Query: 19  MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 198
           MGRVIRAQRKGAGSVF SHT  RKG  K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA 
Sbjct: 1   MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60

Query: 199 VHFRDPYKFKTRKELFIAPKPLH 267
           V FR P++FK +KELF+A + ++
Sbjct: 61  VTFRHPFRFKKQKELFVAAEGMY 83



 Score = 78.2 bits (184), Expect = 5e-15
 Identities = 48/105 (45%), Positives = 67/105 (63%), Gaps = 1/105 (0%)
 Frame = +3

Query: 402 ASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPIFESWKGHTT 581
           ASG++A VI HNPD+  TR+KLPSG+KK++PS  ++M+G VAGGGR +KP+ ++   +  
Sbjct: 129 ASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAY-H 187

Query: 582 SNKVKRN-WLAIM*RGVGH*NLLSIPSRVVVTNHTL*GKASTGQR 713
             +VKRN W  +  RGV   N +  P      NH   G AST +R
Sbjct: 188 KYRVKRNSWPKV--RGVAM-NPVEHPHG--GGNHQHIGHASTVRR 227



 Score = 77.4 bits (182), Expect = 9e-15
 Identities = 32/46 (69%), Positives = 40/46 (86%)
 Frame = +2

Query: 263 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
           YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E  +GDRG LAR
Sbjct: 83  YTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHVGDRGVLAR 128


>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
           protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
          Length = 260

 Score =  118 bits (285), Expect = 3e-27
 Identities = 56/84 (66%), Positives = 68/84 (80%), Gaps = 1/84 (1%)
 Frame = +1

Query: 19  MGRVIRAQRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLA 195
           MGRVIRAQRKGA GSVF SHT  RKG  K RSLDY ER+GY+KG+V +IIHDPGRGAPLA
Sbjct: 1   MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLA 60

Query: 196 VVHFRDPYKFKTRKELFIAPKPLH 267
            V FR P+++  +KELF+A + ++
Sbjct: 61  RVAFRHPFRYMKQKELFVAAEGMY 84



 Score = 79.0 bits (186), Expect = 3e-15
 Identities = 48/104 (46%), Positives = 65/104 (62%)
 Frame = +3

Query: 402 ASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPIFESWKGHTT 581
           ASG++A VI HNP++  TRVKLPSG+KK+LPS  ++M+G VAGGGR +KP  ++   +  
Sbjct: 130 ASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTEKPFLKAGNAY-H 188

Query: 582 SNKVKRNWLAIM*RGVGH*NLLSIPSRVVVTNHTL*GKASTGQR 713
             K KRN   ++ RGV   N +  P      NH   G AST +R
Sbjct: 189 KYKAKRNCWPVV-RGVAM-NPVEHPHG--GGNHQHIGHASTVRR 228



 Score = 76.6 bits (180), Expect = 2e-14
 Identities = 30/46 (65%), Positives = 40/46 (86%)
 Frame = +2

Query: 263 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
           YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E  +GDRG LAR
Sbjct: 84  YTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELHVGDRGALAR 129


>At1g34760.1 68414.m04323 14-3-3 protein GF14 omicron (GRF11)
           identical to SP:Q9S9Z8, 14-3-3-like protein GF14 omicron
           (General regulatory factor 11){Arabidopsis thaliana}
          Length = 255

 Score = 35.5 bits (78), Expect = 0.038
 Identities = 24/74 (32%), Positives = 31/74 (41%)
 Frame = +1

Query: 388 SSGTWPLETSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAG 567
           S+ T    T P+ L   L  SV        P R CH   RA+   + E+D L     K  
Sbjct: 159 SASTELSTTHPIRLGLALNFSVFYYEILNSPERACHLAKRAFDEAIAELDSLNEDSYKDS 218

Query: 568 RAIPQVTRSNVTGW 609
             I Q+ R N+T W
Sbjct: 219 TLIMQLLRDNLTLW 232


>At1g26480.1 68414.m03229 14-3-3 protein GF14 iota (GRF12) identical
           to 14-3-3 protein GF14iota GI:12963453 from [Arabidopsis
           thaliana]
          Length = 268

 Score = 35.5 bits (78), Expect = 0.038
 Identities = 21/66 (31%), Positives = 29/66 (43%)
 Frame = +1

Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
           T P+ L   L  SV        P R CH   +A+   + E+D L+    K    I Q+ R
Sbjct: 172 THPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAIAELDTLSEESYKDSTLIMQLLR 231

Query: 592 SNVTGW 609
            N+T W
Sbjct: 232 DNLTLW 237


>At2g42590.1 68415.m05270 14-3-3 protein GF14 mu (GRF9) identical to
           GF14 mu GI:3551052, SP:Q96299 from [Arabidopsis
           thaliana]
          Length = 263

 Score = 34.3 bits (75), Expect = 0.088
 Identities = 21/66 (31%), Positives = 28/66 (42%)
 Frame = +1

Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
           T P+ L   L  SV        P R CH   +A+   + E+D L     K    I Q+ R
Sbjct: 169 THPIRLGLALNFSVFYYEIMNAPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLR 228

Query: 592 SNVTGW 609
            N+T W
Sbjct: 229 DNLTLW 234


>At4g09000.1 68417.m01487 14-3-3-like protein GF14 chi /  general
           regulatory factor 1 (GRF1) identical to 14-3-3 protein
           GF14 chi chain GI:1702986, SP:P42643 from [Arabidopsis
           thaliana]
          Length = 267

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 20/66 (30%), Positives = 28/66 (42%)
 Frame = +1

Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
           T P+ L   L  SV        P R C+   +A+   + E+D L     K    I Q+ R
Sbjct: 174 THPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLR 233

Query: 592 SNVTGW 609
            N+T W
Sbjct: 234 DNLTLW 239


>At1g78300.1 68414.m09125 14-3-3 protein GF14 omega (GRF2) identical
           to GF14omega isoform GI:487791 from [Arabidopsis
           thaliana]
          Length = 259

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 20/66 (30%), Positives = 28/66 (42%)
 Frame = +1

Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
           T P+ L   L  SV        P R C+   +A+   + E+D L     K    I Q+ R
Sbjct: 169 THPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLR 228

Query: 592 SNVTGW 609
            N+T W
Sbjct: 229 DNLTLW 234


>At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identical
           to GF14 protein phi chain GI:1493805, SP:P46077 from
           [Arabidopsis thaliana]
          Length = 267

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 20/66 (30%), Positives = 28/66 (42%)
 Frame = +1

Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
           T P+ L   L  SV        P R C+   +A+   + E+D L     K    I Q+ R
Sbjct: 175 THPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLR 234

Query: 592 SNVTGW 609
            N+T W
Sbjct: 235 DNLTLW 240


>At1g13540.1 68414.m01587 expressed protein 
          Length = 381

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 17/61 (27%), Positives = 28/61 (45%)
 Frame = -2

Query: 458 SCTLSIRIVSNHSGEVSRGHVPDDLYHPFSLQDCTQWYPQA*LQQASHFQLQELLSFHNK 279
           +C  +I+    HSGE S     DD+    S   C+ + P+A  +Q   F + +    H K
Sbjct: 95  NCYYAIKRHGKHSGEASGSAKEDDI---VSCCFCSSFVPEAKPKQLDPFDMYQQFKIHQK 151

Query: 278 Q 276
           +
Sbjct: 152 E 152


>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
           similar to ribosomal protein L2 [Gossypium arboreum]
           GI:17644114; contains Pfam profile  PF03947: Ribosomal
           Proteins L2, C-terminal domain
          Length = 214

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +2

Query: 308 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
           +G+ MP+G M  GTI+ N+E   G   ++ R
Sbjct: 59  IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVR 89


>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
           similar to ribosomal protein L2 [Gossypium arboreum]
           GI:17644114; contains Pfam profile  PF03947: Ribosomal
           Proteins L2, C-terminal domain
          Length = 214

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +2

Query: 308 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLAR 400
           +G+ MP+G M  GTI+ N+E   G   ++ R
Sbjct: 59  IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVR 89


>At5g38480.1 68418.m04651 14-3-3 protein GF14 psi (GRF3) (RCI1)
           identical to 14-3-3 protein GF14 psi GI:1168200,
           SP:P42644
          Length = 255

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 20/66 (30%), Positives = 27/66 (40%)
 Frame = +1

Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
           T P+ L   L  SV        P R C    +A+   + E+D L     K    I Q+ R
Sbjct: 168 THPIRLGLALNFSVFYYEILNSPDRACSLAKQAFDDAIAELDTLGEESYKDSTLIMQLLR 227

Query: 592 SNVTGW 609
            N+T W
Sbjct: 228 DNLTLW 233


>At3g02520.1 68416.m00240 14-3-3 protein GF14 nu (GRF7) identical to
           14-3-3 protein GF14 nu GI:1531631 from [Arabidopsis
           thaliana]
          Length = 265

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 20/66 (30%), Positives = 27/66 (40%)
 Frame = +1

Query: 412 TSPL*LDTILMLSVQE*SYRLEPRRFCHQGNRAWSVLLLEVDVLTNLFLKAGRAIPQVTR 591
           T P+ L   L  SV        P R C    +A+   + E+D L     K    I Q+ R
Sbjct: 169 THPIRLGLALNFSVFYYEILNSPDRACSLAKQAFDEAISELDTLGEESYKDSTLIMQLLR 228

Query: 592 SNVTGW 609
            N+T W
Sbjct: 229 DNLTLW 234


>At1g18900.2 68414.m02353 pentatricopeptide (PPR) repeat-containing
           protein low similarity to 67kD chloroplastic RNA-binding
           protein, P67 [Arabidopsis thaliana] GI:9755842; contains
           Pfam profile PF01535: PPR repeat
          Length = 860

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 15/52 (28%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
 Frame = -3

Query: 631 PTPRYIMASQLRLTLLLVVWPFQLSKIGLSIRPPPATIPTML-CCLDGRTFL 479
           PT   ++++ LR+  +   +    + + L +RP   T   +L CC DGR+ L
Sbjct: 610 PTCNSLLSTFLRVNKIAEAYELLQNMLALGLRPSLQTYTLLLSCCTDGRSKL 661


>At1g18900.1 68414.m02352 pentatricopeptide (PPR) repeat-containing
           protein low similarity to 67kD chloroplastic RNA-binding
           protein, P67 [Arabidopsis thaliana] GI:9755842; contains
           Pfam profile PF01535: PPR repeat
          Length = 860

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 15/52 (28%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
 Frame = -3

Query: 631 PTPRYIMASQLRLTLLLVVWPFQLSKIGLSIRPPPATIPTML-CCLDGRTFL 479
           PT   ++++ LR+  +   +    + + L +RP   T   +L CC DGR+ L
Sbjct: 610 PTCNSLLSTFLRVNKIAEAYELLQNMLALGLRPSLQTYTLLLSCCTDGRSKL 661


>At3g30520.1 68416.m03863 hypothetical protein 
          Length = 397

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 16/47 (34%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
 Frame = -1

Query: 708 DQWKPYPIMCDW-LPPPVRECSTGFNGQHHVTLWPASYV*PCYLWYG 571
           D+W+  P +  W  PP V +C T  N  H  T   A    P  + YG
Sbjct: 293 DKWRTPPNVAQWGTPPNVAQCRTPPNAPHWGTPPNAHGTTPTNVQYG 339


>At1g53170.1 68414.m06025 ethylene-responsive element-binding factor
           8 / ERF transcription factor 8 (ERF8) identical to ERF
           transcription factor 8 GI:10567108 from [Arabidopsis
           thaliana]
          Length = 185

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 15/51 (29%), Positives = 24/51 (47%)
 Frame = +3

Query: 402 ASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPI 554
           A  NF  ++G +P    T V  P+ A+ + P   +  +G   GGG   + I
Sbjct: 82  AKTNFGVIVGSSPTQSSTVVDSPTAARFITPPHLELSLG---GGGACRRKI 129


>At4g24900.1 68417.m03564 expressed protein
          Length = 421

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 20/67 (29%), Positives = 32/67 (47%)
 Frame = +3

Query: 408 GNFATVIGHNPDAKRTRVKLPSGAKKVLPSRQQSMVGIVAGGGRIDKPIFESWKGHTTSN 587
           G+F++V  +  D  R + K+P   +K+ P+R    VG  A   R    I     GH T +
Sbjct: 305 GDFSSVQLNQSDVARFQAKVPGKNRKLNPNR----VG-AAWAERRKIEIEMEKSGHVTKS 359

Query: 588 KVKRNWL 608
            +  +WL
Sbjct: 360 NIDPDWL 366


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,119,818
Number of Sequences: 28952
Number of extensions: 417048
Number of successful extensions: 1046
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1013
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1044
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -