BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30086
(825 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 48 1e-05
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 48 1e-05
At2g42090.1 68415.m05204 actin, putative similar to SP|P53496 Ac... 30 1.6
At5g24280.1 68418.m02856 expressed protein ; expression supporte... 29 2.8
At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 29 3.7
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 28 6.5
At1g08060.2 68414.m00881 MOM1 identical to MOM1 (mutation in a '... 28 6.5
At1g08060.1 68414.m00880 MOM1 identical to MOM1 (mutation in a '... 28 6.5
At3g29410.1 68416.m03695 terpene synthase/cyclase family protein... 28 8.7
At1g32190.1 68414.m03959 expressed protein 28 8.7
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 47.6 bits (108), Expect = 1e-05
Identities = 27/101 (26%), Positives = 46/101 (45%)
Frame = +1
Query: 205 FMSCNLYYGYVPEVRQKRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDWQVDYE 384
F++ N G++ + RK +F + + G + + G+W++RG E+ D D E
Sbjct: 315 FVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYDME 373
Query: 385 SYDWKKLDPSSEETKKLVQDYFSWNGTDKDGRKFNQGKIFK 507
Y+W K+D S E K+ V + +G K FK
Sbjct: 374 LYEWTKVDISDEAQKERVSQMIE-DAEPFEGEALLDAKCFK 413
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 47.6 bits (108), Expect = 1e-05
Identities = 27/101 (26%), Positives = 46/101 (45%)
Frame = +1
Query: 205 FMSCNLYYGYVPEVRQKRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDWQVDYE 384
F++ N G++ + RK +F + + G + + G+W++RG E+ D D E
Sbjct: 316 FVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYDME 374
Query: 385 SYDWKKLDPSSEETKKLVQDYFSWNGTDKDGRKFNQGKIFK 507
Y+W K+D S E K+ V + +G K FK
Sbjct: 375 LYEWTKVDISDEAQKERVSQMIE-DAEPFEGEALLDAKCFK 414
>At2g42090.1 68415.m05204 actin, putative similar to SP|P53496 Actin
11 {Arabidopsis thaliana}; contains Pfam profile
PF00022: Actin
Length = 366
Score = 30.3 bits (65), Expect = 1.6
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +2
Query: 218 TFITGMFQRLDKSANRLSHPSACLVKTTIPPSPEC 322
T + G+ +R+ K N L PS+ VK +PP EC
Sbjct: 295 TMLHGIKERMTKELNALV-PSSMKVKVVVPPESEC 328
>At5g24280.1 68418.m02856 expressed protein ; expression supported by
MPSS
Length = 1634
Score = 29.5 bits (63), Expect = 2.8
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +3
Query: 645 IKTSVINDFQSPCIHKCSAENDAALHEKMITLAFI 749
+ TS+ +D +S C H S E+D++ E I AF+
Sbjct: 1155 LDTSIHHDEKSGCFHTMSIESDSSSVESAIRYAFV 1189
>At5g15630.1 68418.m01829 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL4 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 431
Score = 29.1 bits (62), Expect = 3.7
Identities = 14/32 (43%), Positives = 16/32 (50%), Gaps = 6/32 (18%)
Frame = +2
Query: 272 HPSACL-----VKTTIPPSPECGCGAE-RSSC 349
HPS C+ TI P P C CG E + SC
Sbjct: 214 HPSCCVSFSSFYNDTITPCPSCACGCENKKSC 245
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 28.3 bits (60), Expect = 6.5
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 1/68 (1%)
Frame = +1
Query: 445 YFSWNGTDKDGRKFNQGKIFK*C-RQTSSQCIHISYNNYKKLYKKETFKCK*VDVGNFRN 621
Y+ W ++ R+ +G+I + C + QC + + NYK +Y++ V V + N
Sbjct: 20 YYEWLTLEE--RRALEGEIVRKCLARNDQQCSFVEHRNYKIVYRRYASLFFMVGVDDDEN 77
Query: 622 CPQFIEFL 645
+EF+
Sbjct: 78 ELAILEFI 85
>At1g08060.2 68414.m00881 MOM1 identical to MOM1 (mutation in a
'Morpheus molecule') [Arabidopsis thaliana]
gi|8132770|gb|AAF73381.1|
Length = 2001
Score = 28.3 bits (60), Expect = 6.5
Identities = 17/53 (32%), Positives = 23/53 (43%)
Frame = +2
Query: 440 RTTSRGTEPTKTVESSTRARYSSNADKRPANVYTFHTITIKNFTKKKHSNVNR 598
R +SRGT TK + ++ R S PA+V +KN T NR
Sbjct: 35 RRSSRGTPSTKVITPASATRKSERLAPSPASVSKKSGGIVKNSTPSSLRRSNR 87
>At1g08060.1 68414.m00880 MOM1 identical to MOM1 (mutation in a
'Morpheus molecule') [Arabidopsis thaliana]
gi|8132770|gb|AAF73381.1|
Length = 2001
Score = 28.3 bits (60), Expect = 6.5
Identities = 17/53 (32%), Positives = 23/53 (43%)
Frame = +2
Query: 440 RTTSRGTEPTKTVESSTRARYSSNADKRPANVYTFHTITIKNFTKKKHSNVNR 598
R +SRGT TK + ++ R S PA+V +KN T NR
Sbjct: 35 RRSSRGTPSTKVITPASATRKSERLAPSPASVSKKSGGIVKNSTPSSLRRSNR 87
>At3g29410.1 68416.m03695 terpene synthase/cyclase family protein
similar to terpene synthase GB:CAA72074 from
[Arabidopsis thaliana], contains Pfam profile: PF01397
terpene synthase family
Length = 603
Score = 27.9 bits (59), Expect = 8.7
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = -2
Query: 221 RLQLMNTFSRVSSGVIWYSGIPKW 150
RLQL T + S +IWY GI KW
Sbjct: 416 RLQL--TIDEIKSLMIWYLGIAKW 437
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 27.9 bits (59), Expect = 8.7
Identities = 13/32 (40%), Positives = 14/32 (43%), Gaps = 1/32 (3%)
Frame = +2
Query: 266 LSHPSACLVKTTIP-PSPECGCGAERSSCSRC 358
L PS K P PS CGCG C +C
Sbjct: 300 LCRPSCSCPKPRCPKPSCSCGCGCGDCGCFKC 331
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,728,695
Number of Sequences: 28952
Number of extensions: 464389
Number of successful extensions: 1179
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1176
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1892353600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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