BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30084 (711 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 161 1e-40 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 105 5e-24 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 102 6e-23 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 75 1e-14 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.66 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.0 SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 27 2.0 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.6 SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces po... 27 3.5 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.5 SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 26 4.6 SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.6 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 8.1 SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 8.1 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 8.1 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 161 bits (390), Expect = 1e-40 Identities = 79/136 (58%), Positives = 94/136 (69%) Frame = +3 Query: 255 LVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKES 434 LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+ Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREA 124 Query: 435 ESCDCLQGFQLXXXXXXXXXXXXXXXXXXXXVKSTPTES*THYSVVPSPKVSDTVVEPYN 614 E+CD LQGFQL + P +SV P+PK SDTVVEPYN Sbjct: 125 EACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYN 184 Query: 615 ATSLSSPA*LKNTDET 662 AT LS ++N+DET Sbjct: 185 AT-LSMHQLVENSDET 199 Score = 113 bits (271), Expect = 3e-26 Identities = 46/64 (71%), Positives = 55/64 (85%) Frame = +1 Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 243 MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 244 PRAI 255 PRA+ Sbjct: 61 PRAV 64 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 105 bits (253), Expect = 5e-24 Identities = 55/142 (38%), Positives = 83/142 (58%), Gaps = 1/142 (0%) Frame = +3 Query: 246 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 422 PR + VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ + Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126 Query: 423 RKESESCDCLQGFQLXXXXXXXXXXXXXXXXXXXXVKSTPTES*THYSVVPSPKVSDTVV 602 R+ +++C LQGF + +S +SV P+P+VS +VV Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV 186 Query: 603 EPYNATSLSSPA*LKNTDETLL 668 EPYN+ L++ A L N+D T + Sbjct: 187 EPYNSV-LTTHATLDNSDCTFM 207 Score = 50.4 bits (115), Expect = 2e-07 Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 6/70 (8%) Frame = +1 Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 225 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 226 SGGKYVPRAI 255 GK+VPR+I Sbjct: 61 GQGKFVPRSI 70 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 102 bits (244), Expect = 6e-23 Identities = 54/142 (38%), Positives = 79/142 (55%), Gaps = 1/142 (0%) Frame = +3 Query: 246 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 422 PR + VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D + Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122 Query: 423 RKESESCDCLQGFQLXXXXXXXXXXXXXXXXXXXXVKSTPTES*THYSVVPSPKVSDTVV 602 R+ +++C LQGF + +S +SV P+P+VS +VV Sbjct: 123 RRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVV 182 Query: 603 EPYNATSLSSPA*LKNTDETLL 668 EPYN+ L++ A L D T + Sbjct: 183 EPYNSV-LTTHATLDLADCTFM 203 Score = 56.4 bits (130), Expect = 4e-09 Identities = 27/66 (40%), Positives = 38/66 (57%), Gaps = 2/66 (3%) Frame = +1 Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 237 MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 238 YVPRAI 255 YVPR+I Sbjct: 61 YVPRSI 66 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 74.9 bits (176), Expect = 1e-14 Identities = 39/124 (31%), Positives = 65/124 (52%), Gaps = 3/124 (2%) Frame = +3 Query: 255 LVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRK 428 L+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ + Sbjct: 66 LIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDR 124 Query: 429 ESESCDCLQGFQLXXXXXXXXXXXXXXXXXXXXVKSTPTES*THYSVVP-SPKVSDTVVE 605 E++ D L+GF L P + YSV P S VSD VV+ Sbjct: 125 EADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQ 184 Query: 606 PYNA 617 PYN+ Sbjct: 185 PYNS 188 Score = 68.9 bits (161), Expect = 7e-13 Identities = 29/63 (46%), Positives = 44/63 (69%) Frame = +1 Query: 67 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 246 REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 247 RAI 255 RAI Sbjct: 63 RAI 65 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 29.1 bits (62), Expect = 0.66 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 2/37 (5%) Frame = +2 Query: 512 LISKIRE--EYPDRIMNTLLSSPLAQSIRHCRRTIQC 616 L+S +R YP+R T L S + QS+ HC + C Sbjct: 197 LLSALRNLGHYPERASKTPLESQIIQSLFHCLNSENC 233 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 2.0 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +3 Query: 366 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 455 A+GH G ELV + D +RK+SE+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 27.5 bits (58), Expect = 2.0 Identities = 13/38 (34%), Positives = 21/38 (55%) Frame = +3 Query: 588 SDTVVEPYNATSLSSPA*LKNTDETLLHRTTKGSITTS 701 +D + P + L + + DETL+H ++GS TTS Sbjct: 289 ADKIPRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTS 326 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +1 Query: 103 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 204 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 295 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = +2 Query: 467 YTFPRWRHRVRYGHLLISKIREEYPDRIMNTLLSS 571 Y R + HLL+ + +PD+I+ TL S+ Sbjct: 121 YYLDRLKFEEAINHLLLPDVLPNFPDKIIETLYSN 155 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 3.5 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 297 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 398 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 967 Score = 26.2 bits (55), Expect = 4.6 Identities = 16/43 (37%), Positives = 21/43 (48%) Frame = +3 Query: 231 RQVRAPRHLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 359 R+V AP+H GT RS +FR ++ FGQ GN Sbjct: 486 REVFAPKH-ARRRLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527 >SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 26.2 bits (55), Expect = 4.6 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = +2 Query: 47 KITYTK*GKSFIYRPANVV 103 +I+YT G SFIYRP N++ Sbjct: 64 EISYT--GTSFIYRPGNII 80 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 8.1 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +3 Query: 318 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 449 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 682 Score = 25.4 bits (53), Expect = 8.1 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 463 NLHIPSVAAPGPVWAPPHLKDP*RVPRQNHEH 558 N+H PS A +++P +P+QNHEH Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH 654 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 8.1 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -3 Query: 355 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 260 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,795,038 Number of Sequences: 5004 Number of extensions: 54610 Number of successful extensions: 166 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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