BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30083 (851 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1952.06c |||DUF1716 family protein|Schizosaccharomyces pombe... 30 0.36 SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ... 30 0.48 SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 28 1.9 SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 27 2.6 SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizo... 26 7.8 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.8 >SPAC1952.06c |||DUF1716 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 564 Score = 30.3 bits (65), Expect = 0.36 Identities = 17/46 (36%), Positives = 27/46 (58%), Gaps = 3/46 (6%) Frame = +3 Query: 597 EQEVYTIPAELKPTNLKQIYVLLER---SKKRIRI*YVSTESRRLQ 725 EQE PA L PT LK++ V LE+ + + +RI Y ++ R ++ Sbjct: 71 EQEAQEEPASLTPTELKRMVVRLEKTINNNQELRIKYSTSPQRFIE 116 >SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar membrane transporter Hmt1|Schizosaccharomyces pombe|chr 3|||Manual Length = 830 Score = 29.9 bits (64), Expect = 0.48 Identities = 20/66 (30%), Positives = 30/66 (45%) Frame = +1 Query: 181 EAMKCKEENCEVGVVLERNSLIVLSLTRPSSASAGGIGTIPSSAGKSHRYASASCVRGRS 360 E +E N + + S + L + SAS GT+ S++ K + A R S Sbjct: 192 ERQLTEESNVNENAISQNPSTVQLGV----SASTSNFGTLKSTSKKPSDKSWAEYFRSFS 247 Query: 361 TLLPYI 378 TLLPY+ Sbjct: 248 TLLPYL 253 >SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccharomyces pombe|chr 1|||Manual Length = 549 Score = 27.9 bits (59), Expect = 1.9 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = -1 Query: 635 WLELSRDRVNFLFRPSFSLVVSNDHGYS 552 WL N L+ P L V +D+GYS Sbjct: 186 WLNYVSTNPNLLYDPELQLFVESDYGYS 213 >SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 926 Score = 27.5 bits (58), Expect = 2.6 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = -1 Query: 626 LSRDRVNFLFRPSFSLVVSNDHGYSCEDCG 537 +SR R ++ FS+ + N H Y C CG Sbjct: 253 ISRCRRCRMYINPFSIFIDNGHRYRCNSCG 282 >SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 287 Score = 25.8 bits (54), Expect = 7.8 Identities = 9/20 (45%), Positives = 12/20 (60%) Frame = -1 Query: 638 CWLELSRDRVNFLFRPSFSL 579 CWL L + VNF+ P F + Sbjct: 27 CWLRLMPETVNFVIVPDFRM 46 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 7.8 Identities = 14/33 (42%), Positives = 20/33 (60%) Frame = +1 Query: 724 KGRLGLKALNVKPKLQMGC*QNSYPTLNVKGNL 822 +G+L LKA N K K Q+G Q + P ++ NL Sbjct: 26 EGKLPLKADNPKKKPQLGTIQANQPIPSIFDNL 58 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,074,748 Number of Sequences: 5004 Number of extensions: 55536 Number of successful extensions: 135 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 422462090 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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