BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30083 (851 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g57600.1 68418.m07196 expressed protein 33 0.32 At2g15420.1 68415.m01764 myosin heavy chain-related 30 1.7 At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 29 3.0 At1g71460.1 68414.m08257 pentatricopeptide (PPR) repeat-containi... 29 5.2 At3g20620.1 68416.m02609 F-box family protein-related contains w... 28 6.9 At1g21740.1 68414.m02721 expressed protein contains Pfam domains... 28 6.9 At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 9.1 >At5g57600.1 68418.m07196 expressed protein Length = 287 Score = 32.7 bits (71), Expect = 0.32 Identities = 22/73 (30%), Positives = 31/73 (42%) Frame = +1 Query: 190 KCKEENCEVGVVLERNSLIVLSLTRPSSASAGGIGTIPSSAGKSHRYASASCVRGRSTLL 369 KC +E E GV E++ L+ L T AS G GT+ + R GR + Sbjct: 188 KCLKEEMECGVKSEKSDLLCLVETAGGVASPGPSGTLQCDLYRPFRLPGILVGDGRLGGI 247 Query: 370 PYILLKYSKLRLR 408 + Y L+LR Sbjct: 248 SGTIAAYESLKLR 260 >At2g15420.1 68415.m01764 myosin heavy chain-related Length = 957 Score = 30.3 bits (65), Expect = 1.7 Identities = 17/56 (30%), Positives = 27/56 (48%) Frame = +1 Query: 202 ENCEVGVVLERNSLIVLSLTRPSSASAGGIGTIPSSAGKSHRYASASCVRGRSTLL 369 EN +G ++ N L ++ + + G +GT P S + R S VRGR +L Sbjct: 317 ENGGLGRLMMENGFDNLVVSGRDTGARGAVGTRPVSRIEERRTGDCSAVRGRQIVL 372 >At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 29.5 bits (63), Expect = 3.0 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +1 Query: 292 GTIPSSAGKSHRYASASCVRGRSTLLPYILLKYSKLRLRRNRILL 426 GT+ S G+ H Y+ C R +LP ++ Y + LRR L+ Sbjct: 79 GTVKSDEGERHTYSVIDCEPKREAILPSVV--YIQKILRRKPFLI 121 >At1g71460.1 68414.m08257 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 689 Score = 28.7 bits (61), Expect = 5.2 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = +1 Query: 193 CKEENCEVGVVLERNSLIVLSLTRPSSASAGGIGTIPS 306 C ENC++ +E L++LS RP S + G + T+ S Sbjct: 493 CYVENCDLRAGIEVFRLMLLSKHRPDSVTMGRVLTVCS 530 >At3g20620.1 68416.m02609 F-box family protein-related contains weak hit to TIGRFAM TIGR01640 : F-box protein interaction domain; similar to F-box protein family, AtFBX8 (GI:20197464) [Arabidopsis thaliana] Length = 391 Score = 28.3 bits (60), Expect = 6.9 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +3 Query: 45 QIVNAGKRGSLAVCAKTRVKLALSSIGSKTCVVSAL 152 Q+ +GK SL VC + L S+ +K C +S L Sbjct: 43 QLAESGKNSSLVVCPSMGKTMKLYSMDAKRCKLSLL 78 >At1g21740.1 68414.m02721 expressed protein contains Pfam domains, PF04782: Protein of unknown function (DUF632) and PF04783: Protein of unknown function (DUF630) Length = 953 Score = 28.3 bits (60), Expect = 6.9 Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 2/47 (4%) Frame = +1 Query: 208 CEVGVVLER--NSLIVLSLTRPSSASAGGIGTIPSSAGKSHRYASAS 342 C+VG ++R + +VL T SS+ + T+PS GK H++ +S Sbjct: 46 CDVGDSIKRFVDEELVLVGTSSSSSPDSPVLTLPSDEGKPHKHKISS 92 >At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|P47823 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus cuniculus}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 27.9 bits (59), Expect = 9.1 Identities = 14/45 (31%), Positives = 23/45 (51%) Frame = +1 Query: 292 GTIPSSAGKSHRYASASCVRGRSTLLPYILLKYSKLRLRRNRILL 426 G++ S G+ H Y+ C R +LP ++ Y + LRR L+ Sbjct: 79 GSVKSDEGERHPYSIIDCEPKREAILPSVV--YIQKILRRKAFLI 121 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,403,088 Number of Sequences: 28952 Number of extensions: 305278 Number of successful extensions: 908 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 877 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 908 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1980143200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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