Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30082
(859 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 138 1e-33
SPBC337.04 |ppk27||serine/threonine protein kinase Ppk27 |Schizo... 28 1.5
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 28 2.0
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 28 2.0
SPCC1235.11 |||conserved eukaryotic protein|Schizosaccharomyces ... 27 2.6
SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 27 2.6
SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dp... 27 3.4
SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 27 4.5
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 27 4.5
SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces... 26 6.0
SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase |Schizosacch... 26 7.9
SPAC4G8.02c |sss1||translocon gamma subunit Sss1|Schizosaccharom... 26 7.9
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 138 bits (333), Expect = 1e-33
Identities = 64/85 (75%), Positives = 77/85 (90%)
Frame = +1
Query: 1 QLFISADRPNVAGLILAGSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIEL 180
Q FI+ D+PNVAG++LAGSADFKTEL QSD+FD RLQ++IIK VDVSYGG+ GFNQAIEL
Sbjct: 208 QHFITDDKPNVAGIVLAGSADFKTELGQSDLFDQRLQSRIIKTVDVSYGGDAGFNQAIEL 267
Query: 181 AAESLQNVKFIQEKKLIGRYFDEIS 255
AA++L NVK++QEKKLI R+FDEIS
Sbjct: 268 AADTLSNVKYVQEKKLIQRFFDEIS 292
Score = 83.0 bits (196), Expect = 5e-17
Identities = 44/87 (50%), Positives = 59/87 (67%), Gaps = 3/87 (3%)
Frame = +3
Query: 258 DTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHLTPEQ-EKD-- 428
D+GKYCFGV DT+ AL+ G+VETL+C+ +LD+ RY K+ N I ++T EQ EKD
Sbjct: 294 DSGKYCFGVVDTMNALQEGAVETLLCFADLDMIRYEFKNSEGN-PVITYMTKEQEEKDST 352
Query: 429 KSHFTDKESGVELELVECQPLLEWLAK 509
S DK++G E+ELV L EWLA+
Sbjct: 353 NSFLLDKDTGAEMELVSSMLLSEWLAE 379
Score = 43.6 bits (98), Expect = 4e-05
Identities = 18/33 (54%), Positives = 25/33 (75%)
Frame = +2
Query: 506 QNYKSFGATLEIITDKSQEGSQFVRGFRKDSAV 604
++YK +GA LE ++D+SQEG QFV+GF AV
Sbjct: 379 EHYKDYGANLEFVSDRSQEGMQFVKGFGGIGAV 411
>SPBC337.04 |ppk27||serine/threonine protein kinase Ppk27
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 413
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 3/38 (7%)
Frame = -1
Query: 400 RIVSWLVACDFNTYLCMSRFSQ---QINVSTDPSSRAR 296
R V LV CD+ +LC S F + N + DPS R R
Sbjct: 337 RYVQRLVRCDYTWHLCKSPFKRSTGNTNDNDDPSWRFR 374
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -2
Query: 630 GKSNFVTEETAESFRKPRTNWLPSWLLSVMISKVAPN 520
G SN T + ++ K ++ +L +WLL + VAP+
Sbjct: 328 GVSNASTAKVLRTYLKTKSEFLRTWLLKALSHLVAPS 364
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.9 bits (59), Expect = 2.0
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 52 GSADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKF 210
G+ DFK DP A+ + ++DVSY E ++AAE+++N+ +
Sbjct: 272 GTIDFKVGKDYWIKEDPPKPARFVFMIDVSY--EASSKGLPKVAAEAIRNILY 322
Score = 25.8 bits (54), Expect = 7.9
Identities = 24/85 (28%), Positives = 40/85 (47%)
Frame = -3
Query: 536 LRWHQTTCSFGQPLQQRLALHKLQLHSALFVGEM*FVLLLFRRQVENCFLVGGMRLQYVS 357
L +HQ T LQ L + L+ +L E+ + + QV N L+ +R+Q+ S
Sbjct: 785 LWFHQRTSPL--LLQDLLGVESLEDIDSLKALELPTLDTVLSVQVRN--LLSSLRMQFPS 840
Query: 356 LYVEVLPADQRLDGSEFESAQCIID 282
+ + Q LDGSE E +++
Sbjct: 841 MALHPQIVRQGLDGSEGEIFSTLVE 865
>SPCC1235.11 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 141
Score = 27.5 bits (58), Expect = 2.6
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -1
Query: 421 SCSGVRWRIVSWLVACDFNTYLCMSRF 341
+ V R ++WL + DF YLC + F
Sbjct: 11 AAQSVTRRFITWLKSPDFRKYLCSTHF 37
>SPAC1296.03c |sxa2||serine carboxypeptidase
Sxa2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 27.5 bits (58), Expect = 2.6
Identities = 27/81 (33%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
Frame = +1
Query: 55 SADFKTELSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIG 234
S+DF L P L K + LV SYG N A L +E N+ F+ + G
Sbjct: 172 SSDFVNALKSFYQKFPHLMKKKLYLVGESYGSIWSANFAEALLSEPSLNINFMGVGIVSG 231
Query: 235 RYFDEISRTQESI-ASVSMIH 294
D TQE I AS+ + H
Sbjct: 232 LTAD--YETQEQITASIWVEH 250
>SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dpb2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 594
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/50 (26%), Positives = 28/50 (56%)
Frame = +3
Query: 375 HATNQETILHLTPEQEKDKSHFTDKESGVELELVECQPLLEWLAKTTSRL 524
H ++ L++ + ++ + F K+ G + +C+PLL+ +AKT R+
Sbjct: 31 HVFTRKYDLNINRDSLQELASFVSKKCGSQWRS-QCEPLLDEIAKTWKRV 79
>SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein
intermediate chain Dic1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 544
Score = 26.6 bits (56), Expect = 4.5
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +1
Query: 109 QAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKK 225
+ K ++ +VSY G N F I++ + QNV F+++ K
Sbjct: 379 ETKAVQPSNVSYEGHNVFISGIDVMTSNSQNV-FLEKNK 416
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 26.6 bits (56), Expect = 4.5
Identities = 19/47 (40%), Positives = 22/47 (46%)
Frame = +3
Query: 267 KYCFGVDDTLRALELGSVETLICWENLDIQRYVLKSHATNQETILHL 407
KY FG RAL +G I W NLD+ Y + T TIL L
Sbjct: 344 KYRFG-----RAL-VGIAPEDIVWSNLDLSMYTRRGKKTISNTILTL 384
>SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 26.2 bits (55), Expect = 6.0
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = -1
Query: 754 LKTRGNLAPFPSKCLPSLNKSVELSVIRLVEFPRSVEAASDWKVQLCNG 608
L+ RG L+ FP+ CL +SV + L+++ S S W++ L G
Sbjct: 673 LRVRGALSKFPTNCL----RSVYSHTVSLMKYLTSA-VISCWRIYLLIG 716
>SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = +3
Query: 468 ELVECQPLLEWLAKTTSRLVP 530
+ ++ QPLLEWLAK + + P
Sbjct: 6 QFLKYQPLLEWLAKHEAYISP 26
>SPAC4G8.02c |sss1||translocon gamma subunit
Sss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 70
Score = 25.8 bits (54), Expect = 7.9
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 6/50 (12%)
Frame = +3
Query: 675 ITDNSTDLFKLGKHFEGNGAKF-PRVFKKGRFPF-----CLYPGFAFLGL 806
+ DN+ DLF++ K+F G+ F R K R F + GF +GL
Sbjct: 1 MADNADDLFQIPKNFYKEGSHFIKRCVKPDRKEFLSISKAVATGFVLMGL 50
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,810,262
Number of Sequences: 5004
Number of extensions: 83304
Number of successful extensions: 243
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 231
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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