BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30055 (803 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 58 1e-09 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 48 1e-06 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 40 3e-04 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 37 0.003 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 35 0.016 SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos... 33 0.063 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 33 0.063 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 32 0.11 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 31 0.25 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 30 0.33 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 30 0.44 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 29 1.0 SPCC757.05c |||acetylornithine deacetylase |Schizosaccharomyces ... 27 2.4 SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 27 2.4 SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosa... 27 4.1 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 26 5.5 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 26 7.2 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 9.5 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 9.5 SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 25 9.5 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 58.4 bits (135), Expect = 1e-09 Identities = 27/79 (34%), Positives = 46/79 (58%) Frame = +2 Query: 356 VPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAIFGPPDTLYQVDTSRHT*N 535 +P+S++ + L + K +Q+ P +GF V L+ + ++FEWEV I GP DTLY+ T + Sbjct: 1 MPSSASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLS 60 Query: 536 SPLTTRIHHHPLGFLTKVW 592 P + + F T++W Sbjct: 61 FPQDYPLMPPKMKFTTEIW 79 Score = 39.5 bits (88), Expect = 5e-04 Identities = 14/18 (77%), Positives = 17/18 (94%) Frame = +3 Query: 594 HPNVYENGDLCISILHPP 647 HPNV+ NG++CISILHPP Sbjct: 80 HPNVHPNGEVCISILHPP 97 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 48.4 bits (110), Expect = 1e-06 Identities = 27/83 (32%), Positives = 41/83 (49%) Frame = +2 Query: 344 MSNTVPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAIFGPPDTLYQVDTSR 523 M++ P+SS R L EY L+E P+ RV L+ +DNLF W GP D++Y Sbjct: 1 MASASPSSS--RRLTKEYSDLREHPIPDIRVNLV-DDNLFHWACTALGPSDSVYAGGKFH 57 Query: 524 HT*NSPLTTRIHHHPLGFLTKVW 592 + PL + F T+++ Sbjct: 58 FSLKFPLDYPFQPPTIEFTTRIY 80 Score = 30.7 bits (66), Expect = 0.25 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +1 Query: 505 SSGYFKAHMKFPPDYPYSPPSI 570 + G F +KFP DYP+ PP+I Sbjct: 52 AGGKFHFSLKFPLDYPFQPPTI 73 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 40.3 bits (90), Expect = 3e-04 Identities = 21/49 (42%), Positives = 27/49 (55%) Frame = +2 Query: 362 TSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAIFGPPDTLYQ 508 + A + L EY +LQ+ PVE K E N+ EW I GPPDT Y+ Sbjct: 3 SKGAYKRLMKEYLALQKNPVELVDAKPATE-NILEWHYIITGPPDTPYE 50 Score = 34.3 bits (75), Expect = 0.021 Identities = 15/48 (31%), Positives = 24/48 (50%) Frame = +1 Query: 511 GYFKAHMKFPPDYPYSPPSIRVFN*SLGIQMYMKTEIFAYRFSTRPWD 654 G + + FPPDYP+ PP+IR+ S Q + + F + W+ Sbjct: 52 GQYHGTLIFPPDYPFKPPAIRMITPSGRFQTNTRLCLSFSDFHPKSWN 99 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 37.1 bits (82), Expect = 0.003 Identities = 17/39 (43%), Positives = 27/39 (69%) Frame = +3 Query: 594 HPNVYENGDLCISILHPPVG*SSVWRTAPANVGNPTQSV 710 HPNVY++G +CISILH P +++ ++ + +P QSV Sbjct: 80 HPNVYKDGTVCISILHAPGDDPNMYESS-SERWSPVQSV 117 Score = 36.7 bits (81), Expect = 0.004 Identities = 18/46 (39%), Positives = 23/46 (50%) Frame = +2 Query: 371 ALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAIFGPPDTLYQ 508 ALR L EYK L E +G ED+ F W+ I GP T ++ Sbjct: 6 ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFE 51 Score = 31.9 bits (69), Expect = 0.11 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +1 Query: 493 GYTLSSGYFKAHMKFPPDYPYSPPSIR 573 G G + A +KFP DYP PP+++ Sbjct: 47 GTPFEGGLYPATLKFPSDYPLGPPTLK 73 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 34.7 bits (76), Expect = 0.016 Identities = 16/52 (30%), Positives = 28/52 (53%) Frame = +2 Query: 362 TSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAIFGPPDTLYQVDT 517 +++A R L ++K +Q++P G + DN+ W I GP DT ++ T Sbjct: 2 STTARRRLMRDFKRMQQDPPAGVSASPVS-DNVMLWNAVIIGPADTPFEDGT 52 Score = 31.5 bits (68), Expect = 0.14 Identities = 11/15 (73%), Positives = 13/15 (86%) Frame = +3 Query: 594 HPNVYENGDLCISIL 638 HPNVY NG+LC+ IL Sbjct: 78 HPNVYANGELCLDIL 92 >SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizosaccharomyces pombe|chr 2|||Manual Length = 953 Score = 32.7 bits (71), Expect = 0.063 Identities = 23/72 (31%), Positives = 36/72 (50%), Gaps = 1/72 (1%) Frame = +3 Query: 588 SGHPNVYENGDLCISILHPPVG*SSVWRTAPANVGNPTQSVRNRYSPSGNLNP-SKEPEH 764 S PN+Y+ + + P+ V+ A+ NP ++ R +PSG+L P S P H Sbjct: 312 SHRPNIYQTLKEIMEMQGTPLKLPDVYGGVNASTYNPPRAPLQR-TPSGSLTPLSSRPAH 370 Query: 765 LSVPGERSMPTV 800 S+P +PTV Sbjct: 371 TSLP---PIPTV 379 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 32.7 bits (71), Expect = 0.063 Identities = 12/18 (66%), Positives = 14/18 (77%) Frame = +1 Query: 517 FKAHMKFPPDYPYSPPSI 570 FK M FP +YPYSPP+I Sbjct: 78 FKISMSFPANYPYSPPTI 95 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 31.9 bits (69), Expect = 0.11 Identities = 15/49 (30%), Positives = 28/49 (57%), Gaps = 1/49 (2%) Frame = +2 Query: 365 SSALRALALEYKSLQEEPVEGFRV-KLLGEDNLFEWEVAIFGPPDTLYQ 508 +S+++ L EY S Q + G + K + + ++F W+ I GP +T Y+ Sbjct: 2 NSSIKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYE 50 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 30.7 bits (66), Expect = 0.25 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +2 Query: 371 ALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAIFGPPDTLY 505 AL+ + E L ++P +G+D LF W+ I GP D+ Y Sbjct: 2 ALKRINRELADLGKDPPSSCSAGPVGDD-LFHWQATIMGPADSPY 45 Score = 27.9 bits (59), Expect = 1.8 Identities = 9/22 (40%), Positives = 13/22 (59%) Frame = +1 Query: 505 SSGYFKAHMKFPPDYPYSPPSI 570 + G F + FP DYP+ PP + Sbjct: 46 AGGVFFLSIHFPTDYPFKPPKV 67 Score = 26.2 bits (55), Expect = 5.5 Identities = 8/15 (53%), Positives = 11/15 (73%) Frame = +3 Query: 594 HPNVYENGDLCISIL 638 HPN+ NG +C+ IL Sbjct: 75 HPNINSNGSICLDIL 89 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 30.3 bits (65), Expect = 0.33 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = +1 Query: 505 SSGYFKAHMKFPPDYPYSPPSIRVFN 582 S G +K H++ P +YP+ PSI N Sbjct: 45 SGGIWKVHVELPSEYPWKSPSIGFVN 70 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 29.9 bits (64), Expect = 0.44 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = +3 Query: 594 HPNVYENGDLCISILHPPVG 653 HPNVY +G +C+SIL+ G Sbjct: 83 HPNVYPSGTVCLSILNEEEG 102 Score = 27.1 bits (57), Expect = 3.1 Identities = 10/21 (47%), Positives = 12/21 (57%) Frame = +1 Query: 511 GYFKAHMKFPPDYPYSPPSIR 573 G +K M FP +YP PP R Sbjct: 56 GLYKLTMAFPEEYPTRPPKCR 76 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 28.7 bits (61), Expect = 1.0 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +1 Query: 493 GYTLSSGYFKAHMKFPPDYPYSPPSI 570 G GYF ++ P DYP+ PP + Sbjct: 45 GTPYEGGYFVVDIEIPIDYPFRPPKM 70 >SPCC757.05c |||acetylornithine deacetylase |Schizosaccharomyces pombe|chr 3|||Manual Length = 400 Score = 27.5 bits (58), Expect = 2.4 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +2 Query: 413 EPVEGFRVKLLGEDNLFEWEVAIFGPP 493 E ++G +K + +WEVAIFG P Sbjct: 186 EEIDGIGMKTVANKLNADWEVAIFGEP 212 >SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 987 Score = 27.5 bits (58), Expect = 2.4 Identities = 16/41 (39%), Positives = 21/41 (51%) Frame = -3 Query: 501 SVSGGPKIATSHSNRLSSPSNLTRKPSTGSSCRLLYSNAKA 379 S S P + S +R SS +LTR+PS S L N +A Sbjct: 85 STSPFPYLKGSFDDRFSSTHSLTRQPSPRSPLTPLKGNTRA 125 >SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1076 Score = 26.6 bits (56), Expect = 4.1 Identities = 12/31 (38%), Positives = 21/31 (67%), Gaps = 1/31 (3%) Frame = +2 Query: 344 MSNTVPTS-SALRALALEYKSLQEEPVEGFR 433 M ++VP + ++L E+ +L E+P+EGFR Sbjct: 22 MFHSVPQNKNSLSVCVAEFTALSEKPLEGFR 52 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 26.2 bits (55), Expect = 5.5 Identities = 10/30 (33%), Positives = 16/30 (53%) Frame = +1 Query: 493 GYTLSSGYFKAHMKFPPDYPYSPPSIRVFN 582 GY G FK ++ +YP+ PP ++ N Sbjct: 63 GY-YKGGKFKFRIQIDDNYPHDPPKVKCLN 91 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 25.8 bits (54), Expect = 7.2 Identities = 14/26 (53%), Positives = 17/26 (65%), Gaps = 2/26 (7%) Frame = -3 Query: 489 GPKIATSHSNRLSSPSN--LTRKPST 418 GP+ TS SN +SP+N T KPST Sbjct: 230 GPRPTTSASNTNTSPANGAPTNKPST 255 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 25.4 bits (53), Expect = 9.5 Identities = 8/18 (44%), Positives = 12/18 (66%) Frame = -3 Query: 612 FHIHLDAQTLVKNPNGWW 559 FH D Q ++KN +G+W Sbjct: 475 FHKKFDVQNVLKNDSGYW 492 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.4 bits (53), Expect = 9.5 Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 4/50 (8%) Frame = +3 Query: 627 ISILHPPVG*SSVWRTAPANVGNPTQSVRNRYSP---SG-NLNPSKEPEH 764 +S L PP +S+ +APA P V Y P SG + P ++P H Sbjct: 904 VSTLPPPPPTASMTASAPAIASPPPPKVGETYHPPTASGTRVPPVQQPSH 953 >SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Manual Length = 835 Score = 25.4 bits (53), Expect = 9.5 Identities = 15/44 (34%), Positives = 18/44 (40%) Frame = -2 Query: 331 IYSTFTTYLNKII*TYHFGTLYAVHVPPGISTRHNNNELEILVN 200 + TF + N+ I TYH H P IS N N L N Sbjct: 526 VEGTFKAFKNRTIHTYHVEGAGGGHAPDIISLVQNPNILPSSTN 569 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,699,998 Number of Sequences: 5004 Number of extensions: 84815 Number of successful extensions: 258 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 229 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 256 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 390427050 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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