Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30038
(661 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 175 6e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 102 5e-23
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 100 2e-22
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 98 1e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 72 9e-14
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 63 3e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 58 1e-09
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 44 1e-05
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 38 0.001
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 37 0.003
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.084
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.45
SPBC530.01 |gyp1||GTPase activating protein Gyp1 |Schizosaccharo... 27 3.2
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 27 3.2
SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schi... 26 4.2
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.2
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 4.2
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 5.5
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 25 7.3
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 175 bits (425), Expect = 6e-45
Identities = 78/85 (91%), Positives = 81/85 (95%)
Frame = +3
Query: 255 LKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 434
LKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FET
Sbjct: 67 LKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFET 126
Query: 435 FNTPAMYVAIQAVLSLYASGRTTGM 509
FN PA YVAIQAVLSLYASGRTTG+
Sbjct: 127 FNAPAFYVAIQAVLSLYASGRTTGI 151
Score = 135 bits (327), Expect = 5e-33
Identities = 61/69 (88%), Positives = 65/69 (94%)
Frame = +1
Query: 61 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 240
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 241 RGILTSNTP 267
RGILT P
Sbjct: 62 RGILTLKYP 70
Score = 95.1 bits (226), Expect = 8e-21
Identities = 43/46 (93%), Positives = 45/46 (97%)
Frame = +2
Query: 509 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERG 646
VLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERG
Sbjct: 152 VLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERG 197
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 102 bits (244), Expect = 5e-23
Identities = 42/85 (49%), Positives = 65/85 (76%)
Frame = +3
Query: 255 LKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 434
+KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE
Sbjct: 67 VKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFER 126
Query: 435 FNTPAMYVAIQAVLSLYASGRTTGM 509
+ +YVAIQAVLSLYA G ++G+
Sbjct: 127 YGFGGVYVAIQAVLSLYAQGLSSGV 151
Score = 61.3 bits (142), Expect = 1e-10
Identities = 26/46 (56%), Positives = 34/46 (73%)
Frame = +2
Query: 509 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERG 646
V+DSGDGV+H VP+YE L H + RLD+AGRD T YL+ +L +G
Sbjct: 152 VVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKG 197
Score = 58.8 bits (136), Expect = 6e-10
Identities = 32/69 (46%), Positives = 42/69 (60%), Gaps = 2/69 (2%)
Frame = +1
Query: 67 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 240
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 241 RGILTSNTP 267
R +L P
Sbjct: 62 RSLLQVKYP 70
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 100 bits (240), Expect = 2e-22
Identities = 43/79 (54%), Positives = 54/79 (68%)
Frame = +3
Query: 255 LKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 434
L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+
Sbjct: 90 LDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFES 149
Query: 435 FNTPAMYVAIQAVLSLYAS 491
FN +Y+A+QAVL+L AS
Sbjct: 150 FNCAGLYIAVQAVLALAAS 168
Score = 53.2 bits (122), Expect = 3e-08
Identities = 23/51 (45%), Positives = 35/51 (68%)
Frame = +2
Query: 491 RSYHRYVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTER 643
RS V+DSGDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R
Sbjct: 177 RSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227
Score = 42.7 bits (96), Expect = 5e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +1
Query: 79 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 228
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 97.9 bits (233), Expect = 1e-21
Identities = 43/86 (50%), Positives = 61/86 (70%), Gaps = 1/86 (1%)
Frame = +3
Query: 255 LKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 431
++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +E
Sbjct: 71 IQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYE 130
Query: 432 TFNTPAMYVAIQAVLSLYASGRTTGM 509
T N PA+ ++Q VL+LYAS RTTG+
Sbjct: 131 TLNVPALSFSLQPVLALYASARTTGI 156
Score = 77.0 bits (181), Expect = 2e-15
Identities = 34/63 (53%), Positives = 44/63 (69%)
Frame = +1
Query: 79 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTS 258
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71
Query: 259 NTP 267
P
Sbjct: 72 QRP 74
Score = 57.2 bits (132), Expect = 2e-09
Identities = 23/46 (50%), Positives = 36/46 (78%)
Frame = +2
Query: 509 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERG 646
VL+ GDG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G
Sbjct: 157 VLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSG 202
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 71.7 bits (168), Expect = 9e-14
Identities = 33/84 (39%), Positives = 48/84 (57%)
Frame = +3
Query: 252 DLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 431
++K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MFE
Sbjct: 64 EIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFE 123
Query: 432 TFNTPAMYVAIQAVLSLYASGRTT 503
+ PA Y+A Q + +ASG+ T
Sbjct: 124 SLRCPATYLAKQETCAAFASGKGT 147
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +1
Query: 64 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 165
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 63.3 bits (147), Expect = 3e-11
Identities = 27/74 (36%), Positives = 44/74 (59%)
Frame = +3
Query: 264 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 443
P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 444 PAMYVAIQAVLSLY 485
P++ I + S Y
Sbjct: 145 PSVAYGIDGLFSFY 158
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 79 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 174
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 29.9 bits (64), Expect = 0.34
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = +2
Query: 509 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKI 631
VL+ G+ SH +P+ G + R+ G + YL+K+
Sbjct: 169 VLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKL 209
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 57.6 bits (133), Expect = 1e-09
Identities = 24/86 (27%), Positives = 49/86 (56%)
Frame = +3
Query: 252 DLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 431
++K +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE
Sbjct: 67 EVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFE 126
Query: 432 TFNTPAMYVAIQAVLSLYASGRTTGM 509
+ PA Y+ QAV +A+ ++T +
Sbjct: 127 QLHVPAFYLTKQAVCVAFANSKSTAL 152
Score = 38.7 bits (86), Expect = 7e-04
Identities = 20/57 (35%), Positives = 33/57 (57%)
Frame = +1
Query: 64 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 234
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 44.4 bits (100), Expect = 1e-05
Identities = 27/91 (29%), Positives = 42/91 (46%), Gaps = 4/91 (4%)
Frame = +3
Query: 249 PDLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMT 416
P+ PI+ G V +W+ ++ W H Y+ L P + +PV L +RE T
Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166
Query: 417 QIMFETFNTPAMYVAIQAVLSLYASGRTTGM 509
Q FE P +A + ++ LYA G G+
Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYAIGILHGL 197
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 37.9 bits (84), Expect = 0.001
Identities = 15/45 (33%), Positives = 29/45 (64%)
Frame = +2
Query: 509 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTER 643
V+DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R
Sbjct: 160 VIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYR 204
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 36.7 bits (81), Expect = 0.003
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = +1
Query: 124 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTSNTPSNTESSL 288
G+ PRA F ++ P H G+++ M KD G+E S +G + TP N S +
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTPENEISEV 554
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.084
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +3
Query: 324 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 467
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.5 bits (63), Expect = 0.45
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -3
Query: 221 PTYESFCPM-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 60
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC530.01 |gyp1||GTPase activating protein Gyp1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 26.6 bits (56), Expect = 3.2
Identities = 19/85 (22%), Positives = 40/85 (47%)
Frame = -1
Query: 628 LHEVVCEVATGQVQTQDGVRECVSLVDGYGVGDTVAGVQHIPVVRPDAYSESTAWMATYM 449
L +++ + + Q G+R V+ + + V+H+ + D S WM +
Sbjct: 364 LSKLLDGIQDNYIHAQPGIRRQVNNLRELTLRIDEPLVKHLQMEGVDFLQFSFRWMNCLL 423
Query: 448 AGVLNVSNMIWVIFSLLALGLRGAS 374
L++SN+I + + +A G++G S
Sbjct: 424 MRELSISNIIRMWDTYMAEGVQGFS 448
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.6 bits (56), Expect = 3.2
Identities = 12/42 (28%), Positives = 24/42 (57%)
Frame = +2
Query: 509 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKIL 634
V+D G + + EG +P++ ++++ G D+T MK+L
Sbjct: 295 VVDMGAQKTSISCVEEGVVVPNSRIKINYGGDDITLLFMKLL 336
>SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase
Gut2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 649
Score = 26.2 bits (55), Expect = 4.2
Identities = 20/71 (28%), Positives = 31/71 (43%)
Frame = +3
Query: 369 LTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGMCWTPATVSPTPYP 548
L+ P NP + + + I+ E V + VLS + R + P+TV P P
Sbjct: 361 LSSVPTNPTPSEDDIQLILKELQKYLVFPVDREDVLSAWCGIRP--LVRDPSTVPPGTDP 418
Query: 549 STRDTHSLTPS 581
+T +T L S
Sbjct: 419 TTGETQGLVRS 429
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +3
Query: 366 LLTEAPLNPKANREKMTQIMFETFN 440
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 4.2
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -3
Query: 224 SPTYESFC--PMPTITP*WRGLPTIEGNTARGASSPAKPAL 108
S T +SF P+PT P LPT NT + P+ PAL
Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 5.5
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 118 NRPCTYRSHCLLPTRQLP 65
NRP YRSH + TR+ P
Sbjct: 323 NRPSNYRSHSSMGTRRSP 340
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.4 bits (53), Expect = 7.3
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = -3
Query: 248 IPLLLCASSPTYESFCPMPTI-TP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAA 72
IP L ++P +F P P++ +P + T +TA ++SP L P + TN +
Sbjct: 363 IPSPLQNTNPAPSTF-PNPSVASPAFPNSSTSNPSTAPASASPLASTLK-PSTANDTNGS 420
Query: 71 TSSS 60
+SSS
Sbjct: 421 SSSS 424
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,968,301
Number of Sequences: 5004
Number of extensions: 67176
Number of successful extensions: 228
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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