BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30036 (699 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 116 4e-27 SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 113 3e-26 SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 2.6 SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 27 3.4 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 3.4 SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 26 6.0 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 6.0 SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosa... 26 6.0 SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 25 7.9 SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharo... 25 7.9 SPAC664.08c |||traub transciption factor family protein |Schizos... 25 7.9 >SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 116 bits (278), Expect = 4e-27 Identities = 53/86 (61%), Positives = 66/86 (76%) Frame = +2 Query: 2 KKGVKKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLAD 181 KKG+KK++VDPF+RKDWYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLAD Sbjct: 13 KKGIKKRVVDPFSRKDWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLAD 72 Query: 182 LQADTDAERSFRKFRLIAEYVQGRMC 259 LQ D E SFRK +L E +QG+ C Sbjct: 73 LQ--KDEEHSFRKVKLRVEDIQGKSC 96 Score = 97.5 bits (232), Expect = 2e-21 Identities = 46/103 (44%), Positives = 66/103 (64%) Frame = +1 Query: 259 LCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 438 L +F+G D+T+DKLR +V+KWQ+ IEAN +KTTDGY+ R+F IGFT++ +KT YA Sbjct: 97 LTSFNGFDMTSDKLRSLVRKWQSTIEANQTIKTTDGYLCRIFVIGFTSRRVNQVKKTTYA 156 Query: 439 QHTQVRAIRKKMCEIITRDVTNSDSGRW*TS*FPDSIAKDIEK 567 Q +Q+RAI +KM ++I P+ I + IEK Sbjct: 157 QSSQIRAIHQKMFQVIQNQANGCSMKELVQKLIPEVIGRAIEK 199 Score = 25.8 bits (54), Expect = 6.0 Identities = 13/26 (50%), Positives = 19/26 (73%) Frame = +3 Query: 585 YPLRDVWHPKVKVLKRAPFSEISKLI 662 YPL++V+ KVK+LK AP + KL+ Sbjct: 205 YPLQNVFVRKVKILK-APKHDAQKLL 229 >SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 113 bits (271), Expect = 3e-26 Identities = 51/86 (59%), Positives = 66/86 (76%) Frame = +2 Query: 2 KKGVKKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLAD 181 KKG+KK++VDPF+RK+WYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLAD Sbjct: 13 KKGIKKRVVDPFSRKEWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLAD 72 Query: 182 LQADTDAERSFRKFRLIAEYVQGRMC 259 LQ D E +FRK +L E +QG+ C Sbjct: 73 LQ--KDEEHAFRKVKLRVEDIQGKSC 96 Score = 99 bits (238), Expect = 3e-22 Identities = 46/103 (44%), Positives = 70/103 (67%) Frame = +1 Query: 259 LCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 438 L +F+G+ +T+DKLR +V+KWQT IEA+ +KTTDGY+ RVF IGFT + + +KT YA Sbjct: 97 LTSFNGLSITSDKLRSLVRKWQTTIEADQTIKTTDGYLCRVFVIGFTRRRANQVKKTTYA 156 Query: 439 QHTQVRAIRKKMCEIITRDVTNSDSGRW*TS*FPDSIAKDIEK 567 Q +Q+RAIR+KM ++I ++ P+ I ++IE+ Sbjct: 157 QSSQIRAIRQKMFQVIQNQTSSCSMRELVQKLIPEVIGREIER 199 >SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosaccharomyces pombe|chr 2|||Manual Length = 2352 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/22 (45%), Positives = 17/22 (77%) Frame = -2 Query: 569 PFSMSLAMESGNQLVHHLPESE 504 PFS+ L +++ ++L+ LPESE Sbjct: 1146 PFSVHLPLDNSSELLKQLPESE 1167 >SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/44 (31%), Positives = 23/44 (52%) Frame = +1 Query: 238 ICARTYVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGY 369 IC YVL NF D +K R + ++ ++N ++ T +GY Sbjct: 95 ICEAIYVLQNFESTDFNKEKERLVSIILESNNKSNNELITKNGY 138 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 1/32 (3%) Frame = -1 Query: 699 PPCLHRLR-RESSQSTSRSPKTGPSSTLSLSD 607 PP ++ R SS STS + + G ST+SLS+ Sbjct: 10 PPTSRSIQSRNSSYSTSSNERIGTPSTISLSE 41 >SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 308 Score = 25.8 bits (54), Expect = 6.0 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 2/37 (5%) Frame = +1 Query: 256 VLCNFHGMDLTTDKLRWMV--KKWQTLIEANIDVKTT 360 V C H MDL + LR +V ++ L+E + D++TT Sbjct: 117 VFCLIHKMDLVQEDLRDLVFEERKAILLETSKDLETT 153 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 25.8 bits (54), Expect = 6.0 Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%) Frame = +3 Query: 384 LHWFHQ*GLLEPTQDVLRPAHSGQ--SNQKENV 476 LHW+ L+ +Q + P H+GQ S + NV Sbjct: 1162 LHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNV 1194 >SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 25.8 bits (54), Expect = 6.0 Identities = 12/42 (28%), Positives = 20/42 (47%) Frame = -1 Query: 279 HAVEVAEHIRPCTYSAINLNLRKDLSASVSACRSARETSKTL 154 H +EV +RP TY + + D+ + + + R E S L Sbjct: 260 HCLEVNSDLRPTTYQLLRSPILSDIRSKLESERVVLEQSDLL 301 >SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe|chr 2|||Manual Length = 882 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/46 (26%), Positives = 21/46 (45%) Frame = +1 Query: 301 RWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 438 ++ W L+ T GYV+R GFT+ ++ + K +A Sbjct: 795 KFATSNWDKLLSRLPVAGTMRGYVVRFVTSGFTHASAIDKIKEFFA 840 >SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharomyces pombe|chr 1|||Manual Length = 406 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 4/64 (6%) Frame = -2 Query: 512 ESELVTSRVMISHIFFLIALT*VCW----A*HVLRWLKESLLVKPMQKTRRTYPSVVFTS 345 ES ++ ++ H+ L+A T W + L +KP+ K + P+ +FT+ Sbjct: 263 ESTSFSTSILFLHVALLVAFTCKHWNKLSRATPFAMVNSMLTLKPLPKLQLATPNFIFTA 322 Query: 344 MLAS 333 + S Sbjct: 323 LATS 326 >SPAC664.08c |||traub transciption factor family protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 452 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 1/34 (2%) Frame = +1 Query: 280 DLTTD-KLRWMVKKWQTLIEANIDVKTTDGYVLR 378 D T D +RWM K Q + N+D K + G +R Sbjct: 351 DSTRDGTVRWMATKKQKQKKENVDTKASKGRKIR 384 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,011,033 Number of Sequences: 5004 Number of extensions: 64463 Number of successful extensions: 216 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 202 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 214 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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