BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30008 (465 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 38 6e-04 SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 1.1 SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 27 1.4 SPCC794.11c |||ENTH domain protein Ent3|Schizosaccharomyces pomb... 26 2.5 SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 26 3.3 SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 25 4.3 SPAC17H9.19c |cdt2|sev1|WD repeat protein Cdt2|Schizosaccharomyc... 25 5.7 >SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosaccharomyces pombe|chr 2|||Manual Length = 479 Score = 38.3 bits (85), Expect = 6e-04 Identities = 17/28 (60%), Positives = 21/28 (75%) Frame = +3 Query: 6 IPTAAAFGGLCIGALSVLADFLGAIGSG 89 IPTAA G +GAL+V +D LGA+GSG Sbjct: 417 IPTAAWLSGAVVGALAVASDLLGALGSG 444 >SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 398 Score = 27.5 bits (58), Expect = 1.1 Identities = 17/74 (22%), Positives = 34/74 (45%) Frame = -1 Query: 333 IFSIKYNDVFTSNTTRYENVHLMMSIRAETTIKTELNAAVRLPLSLRDLEEQCAHAAHLR 154 +F N+V + ++++H ++S A+ I E + L +L A L Sbjct: 223 LFFCSRNEVLSGTNDSHDSIHHIISNNAQLNIPQEYIDVLDLDWKSLELLFVREFCAALG 282 Query: 153 LFLDKDLEVLVNDG 112 + L+ L+++VN G Sbjct: 283 MSLESPLDIVVNAG 296 >SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 1254 Score = 27.1 bits (57), Expect = 1.4 Identities = 14/25 (56%), Positives = 17/25 (68%), Gaps = 2/25 (8%) Frame = -3 Query: 274 TPNDVDSSRNDNKNGA--ERSGPTA 206 TP DVD+S N NKN + ER PT+ Sbjct: 56 TPADVDNSSNVNKNSSSPERELPTS 80 >SPCC794.11c |||ENTH domain protein Ent3|Schizosaccharomyces pombe|chr 3|||Manual Length = 476 Score = 26.2 bits (55), Expect = 2.5 Identities = 13/33 (39%), Positives = 17/33 (51%) Frame = -1 Query: 144 DKDLEVLVNDGDGEQYPSARSRWPRGSRPALTE 46 D D+E DGDG +A + RGSR T+ Sbjct: 229 DNDVEEYNEDGDGGSSDAATANSTRGSRRTTTK 261 >SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1692 Score = 25.8 bits (54), Expect = 3.3 Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 1/49 (2%) Frame = -3 Query: 364 STQQILN-LKSNFFDKVQRCIHIQHYQI*KRTPNDVDSSRNDNKNGAER 221 S++QI N L+ F ++ ++ HY + K + DVDS+ N +E+ Sbjct: 1064 SSEQICNALRRGFLRLNKKLGNVIHYDLRKSSEGDVDSNYVTTMNISEK 1112 >SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 25.4 bits (53), Expect = 4.3 Identities = 9/26 (34%), Positives = 15/26 (57%) Frame = +1 Query: 88 GTGILLAVTIIYQYFEIFVKEQAEMG 165 G G+L+A ++Y FE+ V +G Sbjct: 447 GVGVLIASALVYASFEMIVGANTSLG 472 >SPAC17H9.19c |cdt2|sev1|WD repeat protein Cdt2|Schizosaccharomyces pombe|chr 1|||Manual Length = 490 Score = 25.0 bits (52), Expect = 5.7 Identities = 11/31 (35%), Positives = 15/31 (48%) Frame = -2 Query: 161 ISACSLTKISKYW*MMVTASSIPVPGADGPE 69 IS+CS K W + + P+P A PE Sbjct: 304 ISSCSANSALKLWDLRTVHTVRPLPAATTPE 334 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,874,325 Number of Sequences: 5004 Number of extensions: 35830 Number of successful extensions: 93 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 91 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 93 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 176367270 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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