BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30008 (465 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g34250.1 68415.m04190 protein transport protein sec61, putati... 60 1e-09 At1g29310.1 68414.m03583 protein transport protein sec61, putati... 60 1e-09 At1g78720.1 68414.m09175 protein transport protein sec61, putati... 58 2e-09 At1g31910.1 68414.m03921 GHMP kinase family protein contains TIG... 32 0.22 At3g15720.1 68416.m01992 glycoside hydrolase family 28 protein /... 29 2.0 At5g18360.1 68418.m02160 disease resistance protein (TIR-NBS-LRR... 27 4.7 At4g39753.1 68417.m05629 kelch repeat-containing F-box family pr... 27 4.7 At4g36850.1 68417.m05225 PQ-loop repeat family protein / transme... 27 4.7 At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein... 27 6.2 At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein... 27 6.2 At5g22355.1 68418.m02608 DC1 domain-containing protein contains ... 27 6.2 At4g36280.1 68417.m05159 ATP-binding region, ATPase-like domain-... 27 8.3 At4g00150.1 68417.m00015 scarecrow-like transcription factor 6 (... 27 8.3 >At2g34250.1 68415.m04190 protein transport protein sec61, putative similar to PfSec61 [Plasmodium falciparum] GI:3057044; contains Pfam profile PF00344: eubacterial secY protein Length = 475 Score = 59.7 bits (138), Expect = 1e-09 Identities = 26/29 (89%), Positives = 29/29 (100%) Frame = +3 Query: 3 YIPTAAAFGGLCIGALSVLADFLGAIGSG 89 YIPTAAAFGG+CIGAL+VLADF+GAIGSG Sbjct: 417 YIPTAAAFGGVCIGALTVLADFMGAIGSG 445 Score = 45.2 bits (102), Expect = 2e-05 Identities = 23/32 (71%), Positives = 25/32 (78%), Gaps = 1/32 (3%) Frame = +1 Query: 73 GPSAPGTGILLAVTIIYQYFEIFVKEQA-EMG 165 G GTGILLAVTIIYQYFE F KE+A E+G Sbjct: 440 GAIGSGTGILLAVTIIYQYFETFEKEKASELG 471 >At1g29310.1 68414.m03583 protein transport protein sec61, putative similar to PfSec61 [Plasmodium falciparum] GI:3057044; contains Pfam profile PF00344: eubacterial secY protein Length = 475 Score = 59.7 bits (138), Expect = 1e-09 Identities = 26/29 (89%), Positives = 29/29 (100%) Frame = +3 Query: 3 YIPTAAAFGGLCIGALSVLADFLGAIGSG 89 YIPTAAAFGG+CIGAL+VLADF+GAIGSG Sbjct: 417 YIPTAAAFGGVCIGALTVLADFMGAIGSG 445 Score = 45.2 bits (102), Expect = 2e-05 Identities = 23/32 (71%), Positives = 25/32 (78%), Gaps = 1/32 (3%) Frame = +1 Query: 73 GPSAPGTGILLAVTIIYQYFEIFVKEQA-EMG 165 G GTGILLAVTIIYQYFE F KE+A E+G Sbjct: 440 GAIGSGTGILLAVTIIYQYFETFEKEKASELG 471 >At1g78720.1 68414.m09175 protein transport protein sec61, putative similar to SP|P38377 Protein transport protein Sec61 alpha subunit isoform 1 (Sec61 alpha- 1) {Canis familiaris}; contains Pfam profile PF00344: eubacterial secY protein Length = 475 Score = 58.4 bits (135), Expect = 2e-09 Identities = 26/29 (89%), Positives = 28/29 (96%) Frame = +3 Query: 3 YIPTAAAFGGLCIGALSVLADFLGAIGSG 89 YIPTAAAFGGLCIGAL+VLAD +GAIGSG Sbjct: 417 YIPTAAAFGGLCIGALTVLADLMGAIGSG 445 Score = 45.2 bits (102), Expect = 2e-05 Identities = 23/32 (71%), Positives = 25/32 (78%), Gaps = 1/32 (3%) Frame = +1 Query: 73 GPSAPGTGILLAVTIIYQYFEIFVKEQA-EMG 165 G GTGILLAVTIIYQYFE F KE+A E+G Sbjct: 440 GAIGSGTGILLAVTIIYQYFETFEKEKASELG 471 >At1g31910.1 68414.m03921 GHMP kinase family protein contains TIGRFAM profile TIGR01219: phosphomevalonate kinase; contains Pfam PF00288: GHMP kinases putative ATP-binding protein domain; similar to Phosphomevalonate kinase (EC 2.7.4.2) (Swiss-Prot:P24521) [Saccharomyces cerevisiae] Length = 505 Score = 31.9 bits (69), Expect = 0.22 Identities = 18/65 (27%), Positives = 28/65 (43%) Frame = +3 Query: 3 YIPTAAAFGGLCIGALSVLADFLGAIGSGHWDTARRHHHLPVLRDLCQGTSGDGRHEHTA 182 + P +F + + L L + +G I G WD R LP L +L G G G + Sbjct: 256 FSPEVLSFAQVAVTGLP-LNEVIGTILKGKWDNKRTEFSLPPLMNLFLGEPGSGGSSTPS 314 Query: 183 LLSLV 197 ++ V Sbjct: 315 MVGAV 319 >At3g15720.1 68416.m01992 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase [Cucumis sativus] GI:6624205; contains Pfam profile PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 456 Score = 28.7 bits (61), Expect = 2.0 Identities = 19/40 (47%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Frame = -1 Query: 138 DLEVLVNDGDGEQYPSARSRWP-RGSRP-ALTEPRCTVLR 25 D+E LV +GDGE S W +GSRP AL C LR Sbjct: 111 DIEGLVIEGDGEINGQGSSWWEHKGSRPTALKFRSCNNLR 150 >At5g18360.1 68418.m02160 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 900 Score = 27.5 bits (58), Expect = 4.7 Identities = 15/34 (44%), Positives = 23/34 (67%), Gaps = 1/34 (2%) Frame = +1 Query: 88 GTGILLAVTI-IYQYFEIFVKEQAEMGGMSTLLF 186 GTG +L +++ I + E+F+ E+A GGM LLF Sbjct: 527 GTGAVLGISLDISKINELFLNERA-FGGMHNLLF 559 >At4g39753.1 68417.m05629 kelch repeat-containing F-box family protein contains F-box domain Pfam:PF00646 and Kelch motif Pfam:PF01344 Length = 390 Score = 27.5 bits (58), Expect = 4.7 Identities = 10/30 (33%), Positives = 15/30 (50%) Frame = -1 Query: 120 NDGDGEQYPSARSRWPRGSRPALTEPRCTV 31 ND Y R +W ++P L + RC+V Sbjct: 250 NDSKDSVYDPIREKWNVAAKPQLNDSRCSV 279 >At4g36850.1 68417.m05225 PQ-loop repeat family protein / transmembrane family protein similar to SP|Q10482 Seven transmembrane protein 1 {Schizosaccharomyces pombe}; contains Pfam profile PF04193: PQ loop repeat Length = 374 Score = 27.5 bits (58), Expect = 4.7 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = -1 Query: 234 TELNAAVRLPLSLRDLEEQCAHAAHLRLFLDKDLE 130 T L A+ LPL + L E+ AHA+ RL ++ +E Sbjct: 220 TFLAASASLPLQAKSLAEKYAHASSRRLLNERIVE 254 >At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein (ZWICHEL) identical to kinesin-like protein GI:2224925 from [Arabidopsis thaliana] Length = 1260 Score = 27.1 bits (57), Expect = 6.2 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +3 Query: 45 ALSVLADFLGAIGSGHWDTARRHHHLPVL 131 +LS L D +GA+ SG+ R+H L +L Sbjct: 1141 SLSALGDVIGALSSGNQHIPYRNHKLTML 1169 >At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein (ZWICHEL) identical to kinesin-like protein GI:2224925 from [Arabidopsis thaliana] Length = 1259 Score = 27.1 bits (57), Expect = 6.2 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +3 Query: 45 ALSVLADFLGAIGSGHWDTARRHHHLPVL 131 +LS L D +GA+ SG+ R+H L +L Sbjct: 1140 SLSALGDVIGALSSGNQHIPYRNHKLTML 1168 >At5g22355.1 68418.m02608 DC1 domain-containing protein contains Pfam PF03107: DC1 domain; similar to ULI3 [Arabidopsis thaliana] GI:17225050 Length = 664 Score = 27.1 bits (57), Expect = 6.2 Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 1/47 (2%) Frame = -3 Query: 445 KFISY-KLAHHPRTRRHAHNTTPVGALPSTQQILNLKSNFFDKVQRC 308 +FI + K A+HP+ +RH +T P + + K F V RC Sbjct: 417 RFILHEKCANHPKKKRHVFHTKPFTLWSRPPRTFHSKDFRFYDVFRC 463 >At4g36280.1 68417.m05159 ATP-binding region, ATPase-like domain-containing protein low similarity to microrchidia [Mus musculus] GI:5410255; contains Pfam profile PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein Length = 626 Score = 26.6 bits (56), Expect = 8.3 Identities = 18/49 (36%), Positives = 24/49 (48%) Frame = -1 Query: 288 RYENVHLMMSIRAETTIKTELNAAVRLPLSLRDLEEQCAHAAHLRLFLD 142 R EN+ L M R E IK E + ++LEE + AHL L +D Sbjct: 571 RQENLQLFM--RCEEYIKKENETEQTVKSLEKELEEFKSKCAHLALLVD 617 >At4g00150.1 68417.m00015 scarecrow-like transcription factor 6 (SCL6) Length = 558 Score = 26.6 bits (56), Expect = 8.3 Identities = 14/39 (35%), Positives = 18/39 (46%) Frame = +3 Query: 72 GAIGSGHWDTARRHHHLPVLRDLCQGTSGDGRHEHTALL 188 GAIG G W+ H H + L G S D E ++L Sbjct: 91 GAIGLGDWEEQVPHDHEQSILGLIMGDSTDPSLELNSIL 129 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,038,422 Number of Sequences: 28952 Number of extensions: 201458 Number of successful extensions: 625 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 604 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 625 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 782033640 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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