BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20998 (736 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g55940.1 68418.m06977 expressed protein contains Pfam PF03665... 42 3e-04 At5g18810.1 68418.m02235 SC35-like splicing factor, 28 kD (SCL28... 27 9.8 >At5g55940.1 68418.m06977 expressed protein contains Pfam PF03665: Uncharacterised protein family (UPF0172) Length = 208 Score = 42.3 bits (95), Expect = 3e-04 Identities = 26/70 (37%), Positives = 38/70 (54%), Gaps = 1/70 (1%) Frame = +1 Query: 301 LDIVDAIPLFHHSHYVSPMAEVALTQIETIAQSENRVIAGYYAACENFRDNIVERCPGLK 480 ++I D++PLFH + + P E++L IE ++ I GY+ A E F D VE C K Sbjct: 49 VEISDSVPLFHSNLALLPPLEISLIMIEEHYVAQGLSIVGYFHANERFDD--VELCGVAK 106 Query: 481 -IAEKIVEYF 507 I + I YF Sbjct: 107 NIGDHISRYF 116 Score = 30.3 bits (65), Expect = 1.4 Identities = 12/26 (46%), Positives = 18/26 (69%) Frame = +2 Query: 182 IETGAYAKIILHAAKYPHCAVNGVLL 259 I AY K++LH+ ++ AVNGVL+ Sbjct: 13 ISQNAYIKLVLHSLRHKTAAVNGVLV 38 >At5g18810.1 68418.m02235 SC35-like splicing factor, 28 kD (SCL28) nearly identical to SC35-like splicing factor SCL28, 28 kD [Arabidopsis thaliana] GI:9843655; contains Pfam profile PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) Length = 236 Score = 27.5 bits (58), Expect = 9.8 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = +3 Query: 333 PQPLRLTNGRSSTHTD*NHSSIRKP 407 PQ +R TNG S H D +S R P Sbjct: 130 PQEMRTTNGTSGRHGDYKRTSHRSP 154 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,765,995 Number of Sequences: 28952 Number of extensions: 283384 Number of successful extensions: 633 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 623 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 633 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1614253080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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