Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20990
(768 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 170 1e-42
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 169 2e-42
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 163 1e-40
At2g33200.1 68415.m04067 F-box family protein contains F-box dom... 28 6.0
At1g05670.1 68414.m00588 UDP-glucoronosyl/UDP-glucosyl transfera... 28 6.0
At1g53900.1 68414.m06136 eukaryotic translation initiation facto... 28 7.9
At1g53880.1 68414.m06133 eukaryotic translation initiation facto... 28 7.9
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 170 bits (413), Expect = 1e-42
Identities = 90/170 (52%), Positives = 105/170 (61%), Gaps = 1/170 (0%)
Frame = +1
Query: 253 PHRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGT 432
P+RD TDD+VTI++A A LK+NV IKCATITPDE RV EF LKKMW SPNGTIRNIL GT
Sbjct: 49 PNRDFTDDKVTIETAEATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGT 108
Query: 433 VFREPILCQSIPRVVPGWTKPIVIGLMRTATNTRRKILLYRNPERLNLFTLHEMVRQRGV 612
VFREPI+C++IPR+VPGWTKPI IG R L+ P +L L Q+
Sbjct: 109 VFREPIICRNIPRLVPGWTKPICIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTE 168
Query: 613 YYMI-LKLRALLWACTIPTNRFGPLPIPVFQVALQKKWPLYLSTKNTILK 759
+ + + A A QKKWPLYLSTKNTILK
Sbjct: 169 FEVFNFTGGGVALAMYNTDESIRAFAESSMYTAYQKKWPLYLSTKNTILK 218
Score = 69.3 bits (162), Expect = 3e-12
Identities = 33/75 (44%), Positives = 49/75 (65%), Gaps = 1/75 (1%)
Frame = +3
Query: 498 CDRPHAHGDQYKAQDFVVPKPGKVELVYTTRDGTTERRV-LYDFKTPGVAMGMYNTDESI 674
C HA GDQY+A D +V +PGK++LV+ + + +++F GVA+ MYNTDESI
Sbjct: 131 CIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALAMYNTDESI 190
Query: 675 RSFAHSSFSGSFTEK 719
R+FA SS ++ +K
Sbjct: 191 RAFAESSMYTAYQKK 205
Score = 65.7 bits (153), Expect = 3e-11
Identities = 26/45 (57%), Positives = 37/45 (82%)
Frame = +2
Query: 119 KRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGL 253
+++ PVVEMDGDEMTR++W IK++LIFP++++D YFDLGL
Sbjct: 4 EKIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGL 48
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 169 bits (411), Expect = 2e-42
Identities = 85/170 (50%), Positives = 105/170 (61%)
Frame = +1
Query: 253 PHRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGT 432
PHRDATDD+VTI+SA A K+NV IKCATITPDE RV EF LK+MW SPNGTIRNIL GT
Sbjct: 49 PHRDATDDKVTIESAEATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGT 108
Query: 433 VFREPILCQSIPRVVPGWTKPIVIGLMRTATNTRRKILLYRNPERLNLFTLHEMVRQRGV 612
VFREPI+C+++P++VPGWTKPI IG R + + P +L + + +
Sbjct: 109 VFREPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETE 168
Query: 613 YYMILKLRALLWACTIPTNRFGPLPIPVFQVALQKKWPLYLSTKNTILKR 762
+ + A A +KKWPLYLSTKNTILK+
Sbjct: 169 VFTFTGEGGVAMAMYNTDESIRAFADASMNTAYEKKWPLYLSTKNTILKK 218
Score = 73.7 bits (173), Expect = 1e-13
Identities = 35/74 (47%), Positives = 47/74 (63%)
Frame = +3
Query: 498 CDRPHAHGDQYKAQDFVVPKPGKVELVYTTRDGTTERRVLYDFKTPGVAMGMYNTDESIR 677
C HA GDQY+A D V+ PGK+ + + +DG TE V GVAM MYNTDESIR
Sbjct: 131 CIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMYNTDESIR 190
Query: 678 SFAHSSFSGSFTEK 719
+FA +S + ++ +K
Sbjct: 191 AFADASMNTAYEKK 204
Score = 66.5 bits (155), Expect = 2e-11
Identities = 26/45 (57%), Positives = 37/45 (82%)
Frame = +2
Query: 119 KRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGL 253
+++ A P+VEMDGDEMTR++W IK++LI P+V++D YFDLGL
Sbjct: 4 EKIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGL 48
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 163 bits (396), Expect = 1e-40
Identities = 87/170 (51%), Positives = 105/170 (61%), Gaps = 1/170 (0%)
Frame = +1
Query: 256 HRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTV 435
+RDATDD+VT++SA A LK+NV IKCATITPDE RV+EF LK MW SPNGTIRNIL GTV
Sbjct: 120 NRDATDDKVTVESAEAALKYNVAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTV 179
Query: 436 FREPILCQSIPRVVPGWTKPIVIGLMRTATNTRRKILLYRNPERLNL-FTLHEMVRQRGV 612
FREPI+C +IPR+VPGW KPI IG R + + P +L + F + +
Sbjct: 180 FREPIMCSNIPRLVPGWEKPICIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVEL 239
Query: 613 YYMILKLRALLWACTIPTNRFGPLPIPVFQVALQKKWPLYLSTKNTILKR 762
K + A +AL KKWPLYLSTKNTILK+
Sbjct: 240 DVYDFKGPGVALAMYNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKK 289
Score = 82.2 bits (194), Expect = 3e-16
Identities = 38/79 (48%), Positives = 53/79 (67%), Gaps = 1/79 (1%)
Frame = +3
Query: 486 DETYCDRPHAHGDQYKAQDFVVPKPGKVELVYTTRDGTTERRV-LYDFKTPGVAMGMYNT 662
++ C HA GDQY+A D V+ PGK+++V+ DG + +YDFK PGVA+ MYN
Sbjct: 197 EKPICIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALAMYNV 256
Query: 663 DESIRSFAHSSFSGSFTEK 719
DESIR+FA SS + + T+K
Sbjct: 257 DESIRAFAESSMAMALTKK 275
Score = 68.1 bits (159), Expect = 6e-12
Identities = 27/48 (56%), Positives = 37/48 (77%)
Frame = +2
Query: 110 GTAKRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGL 253
G + R+ P+VEMDGDEMTR++W+ IKE+LI PY+ +D YFDLG+
Sbjct: 71 GGSDRIQVQNPIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGI 118
>At2g33200.1 68415.m04067 F-box family protein contains F-box domain
Pfam:PF00646
Length = 376
Score = 28.3 bits (60), Expect = 6.0
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +3
Query: 375 VEENVAKPERNDP*HFGRYSIPRTDIVSEYPEGCAWLDE 491
+E ++ ER + ++ I RTDI+S + C W++E
Sbjct: 119 MESSILGKERLEKEVAWKHFIERTDIISTKKQACLWINE 157
>At1g05670.1 68414.m00588 UDP-glucoronosyl/UDP-glucosyl transferase
family protein similar to UDP-glucose:salicylic acid
glucosyltransferase [Nicotiana tabacum] GI:7385017;
contains Pfam profiles PF00201: UDP-glucoronosyl and
UDP-glucosyl transferase, PF01535: PPR repeat
Length = 1184
Score = 28.3 bits (60), Expect = 6.0
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -3
Query: 724 AIFSVKLPEKLEWAKDRIDSSVLYMPIATPGVLK 623
++F K+ E +EW + SSV+Y+ + VLK
Sbjct: 251 SLFGAKIAECMEWLNSKQPSSVVYVSFGSLVVLK 284
>At1g53900.1 68414.m06136 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|Q64270 Translation initiation factor eIF-2B alpha
subunit {Rattus norvegicus}; contains Pfam profiles
PF04525: Protein of unknown function (DUF567), PF01008:
Initiation factor 2 subunit family
Length = 644
Score = 27.9 bits (59), Expect = 7.9
Identities = 17/48 (35%), Positives = 24/48 (50%)
Frame = +3
Query: 540 DFVVPKPGKVELVYTTRDGTTERRVLYDFKTPGVAMGMYNTDESIRSF 683
DF VP P KVE+ + RD T + + F GV +DE I+ +
Sbjct: 596 DFSVPVPPKVEVERSARDYTPPQYLTLLFTDLGVLTPSVVSDELIQLY 643
>At1g53880.1 68414.m06133 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|Q64270 Translation initiation factor eIF-2B alpha
subunit {Rattus norvegicus}; contains Pfam profiles
PF04525: Protein of unknown function (DUF567), PF01008:
Initiation factor 2 subunit family
Length = 637
Score = 27.9 bits (59), Expect = 7.9
Identities = 17/48 (35%), Positives = 24/48 (50%)
Frame = +3
Query: 540 DFVVPKPGKVELVYTTRDGTTERRVLYDFKTPGVAMGMYNTDESIRSF 683
DF VP P KVE+ + RD T + + F GV +DE I+ +
Sbjct: 589 DFSVPVPPKVEVERSARDYTPPQYLTLLFTDLGVLTPSVVSDELIQLY 636
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,529,257
Number of Sequences: 28952
Number of extensions: 374644
Number of successful extensions: 881
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 879
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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