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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20983
         (756 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ...    61   7e-10
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ...    60   2e-09
At1g02930.1 68414.m00260 glutathione S-transferase, putative sim...    42   4e-04
At4g02520.1 68417.m00345 glutathione S-transferase, putative           39   0.003
At2g02930.1 68415.m00241 glutathione S-transferase, putative           39   0.003
At1g02950.2 68414.m00263 glutathione S-transferase, putative sim...    38   0.005
At1g02950.1 68414.m00262 glutathione S-transferase, putative sim...    38   0.005
At1g02920.1 68414.m00259 glutathione S-transferase, putative sim...    38   0.007
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do...    36   0.022
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim...    34   0.089
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF...    33   0.15 
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id...    32   0.47 
At3g25670.1 68416.m03195 leucine-rich repeat family protein cont...    29   2.5  
At3g14120.2 68416.m01786 expressed protein similar to Nuclear po...    29   2.5  
At3g14120.1 68416.m01785 expressed protein similar to Nuclear po...    29   2.5  
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-...    29   2.5  
At1g13230.1 68414.m01535 leucine-rich repeat family protein cont...    29   3.3  
At5g07270.1 68418.m00829 ankyrin repeat family protein contains ...    29   4.4  
At1g79280.1 68414.m09242 expressed protein weak similarity to Nu...    29   4.4  
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon...    29   4.4  
At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / gluta...    28   7.7  
At3g28910.1 68416.m03608 myb family transcription factor (MYB30)...    28   7.7  
At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1...    28   7.7  
At1g68780.1 68414.m07862 leucine-rich repeat family protein cont...    28   7.7  

>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
           eEF-1B gamma, putative Similar to elongation factor
           1-gamma (gb|EF1G_XENLA). ESTs
           gb|T20564,gb|T45940,gb|T04527 come from this gene
          Length = 414

 Score = 61.3 bits (142), Expect = 7e-10
 Identities = 35/87 (40%), Positives = 47/87 (54%), Gaps = 4/87 (4%)
 Frame = +1

Query: 1   VKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA----NESLRG 168
           + V  +F  G TNK+  FLK  P GKVP  E+ +G V   ESNAIA YV+    + SL G
Sbjct: 28  IDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETPEGSVF--ESNAIARYVSRLNGDNSLNG 85

Query: 169 GDLATQARVWQWASWSDSELLPASCAW 249
             L   A++ QW  +S  E+  +   W
Sbjct: 86  SSLIEYAQIEQWIDFSSLEIYASILRW 112



 Score = 51.6 bits (118), Expect = 5e-07
 Identities = 30/81 (37%), Positives = 42/81 (51%)
 Frame = +3

Query: 255 PYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHV 434
           P +G M ++    E A S L  AL  L+ HL + T+LV   ITLAD+I    L   F  V
Sbjct: 115 PRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATV 174

Query: 435 LDPSVRSSLINVQRWFLTVAH 497
           +     S   +V+R+F TV +
Sbjct: 175 MTKKFTSEFPHVERYFWTVVN 195


>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
           eEF-1B gamma, putative similar to elongation factor 1B
           gamma GI:3868758 from [Oryza sativa]
          Length = 413

 Score = 60.1 bits (139), Expect = 2e-09
 Identities = 34/87 (39%), Positives = 47/87 (54%), Gaps = 4/87 (4%)
 Frame = +1

Query: 1   VKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA----NESLRG 168
           ++ + +F  G TNKS +FLK  P GKVP  E+ +G +   ESNAIA YV+    + SL G
Sbjct: 28  IEESADFQMGVTNKSPEFLKMNPIGKVPVLETPEGPIF--ESNAIARYVSRKNGDNSLNG 85

Query: 169 GDLATQARVWQWASWSDSELLPASCAW 249
             L   A + QW  +S  E+      W
Sbjct: 86  SSLIEYAHIEQWIDFSSLEIDANMLKW 112



 Score = 49.2 bits (112), Expect = 3e-06
 Identities = 27/81 (33%), Positives = 42/81 (51%)
 Frame = +3

Query: 255 PYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHV 434
           P +G   F+    E A S L   L+ L+ HL + TFLV   +TLAD++    L   F  V
Sbjct: 115 PRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATV 174

Query: 435 LDPSVRSSLINVQRWFLTVAH 497
           +     S+  +V+R+F T+ +
Sbjct: 175 MTKKFTSAFPHVERYFWTMVN 195


>At1g02930.1 68414.m00260 glutathione S-transferase, putative
           similar to glutathione S-transferase GI:860955 from
           [Hyoscyamus muticus]
          Length = 208

 Score = 41.9 bits (94), Expect = 4e-04
 Identities = 23/53 (43%), Positives = 33/53 (62%), Gaps = 1/53 (1%)
 Frame = +1

Query: 37  NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVANE-SLRGGDLATQAR 192
           +K E F+ + P GKVPAFE  D K+   ES AI  Y+A+E S +G +L +  +
Sbjct: 41  HKKEPFILRNPFGKVPAFEDGDFKIF--ESRAITQYIAHEFSDKGNNLLSTGK 91


>At4g02520.1 68417.m00345 glutathione S-transferase, putative
          Length = 212

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 20/39 (51%), Positives = 26/39 (66%)
 Frame = +1

Query: 37  NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 153
           +K E FL + P G+VPAFE  DG + L ES AI  Y+A+
Sbjct: 41  HKKEPFLSRNPFGQVPAFE--DGDLKLFESRAITQYIAH 77


>At2g02930.1 68415.m00241 glutathione S-transferase, putative
          Length = 212

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 20/39 (51%), Positives = 26/39 (66%)
 Frame = +1

Query: 37  NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 153
           +K E FL + P G+VPAFE  DG + L ES AI  Y+A+
Sbjct: 41  HKKEPFLSRNPFGQVPAFE--DGDLKLFESRAITQYIAH 77


>At1g02950.2 68414.m00263 glutathione S-transferase, putative
           similar to glutathione-S-transferase GI:169887 from
           [Silene vulgaris]
          Length = 245

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
 Frame = +1

Query: 37  NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 177
           +K+E FL   P G+VP FE  DG V L ES AI  Y+A   S RG  L
Sbjct: 64  HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 109


>At1g02950.1 68414.m00262 glutathione S-transferase, putative
           similar to glutathione-S-transferase GI:169887 from
           [Silene vulgaris]
          Length = 243

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
 Frame = +1

Query: 37  NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 177
           +K+E FL   P G+VP FE  DG V L ES AI  Y+A   S RG  L
Sbjct: 62  HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 107


>At1g02920.1 68414.m00259 glutathione S-transferase, putative
           similar to glutathione S-transferase GI:860955 from
           [Hyoscyamus muticus]; supported by cDNA GI:443697.
          Length = 209

 Score = 37.9 bits (84), Expect = 0.007
 Identities = 20/39 (51%), Positives = 25/39 (64%)
 Frame = +1

Query: 37  NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 153
           +K E F+ + P GKVPAFE  DG   L ES AI  Y+A+
Sbjct: 41  HKKEPFIFRNPFGKVPAFE--DGDFKLFESRAITQYIAH 77


>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
           domain-containing protein contains Pfam domain PF00043:
           Glutathione S-transferase, C-terminal domain
          Length = 399

 Score = 36.3 bits (80), Expect = 0.022
 Identities = 16/46 (34%), Positives = 31/46 (67%)
 Frame = +3

Query: 279 NKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLL 416
           +++  + A ++L + L  ++ HL +  +L  ER+TLADV +F+TL+
Sbjct: 261 SQEAYDGAVNELFSTLDEIEDHLGSNRYLCGERLTLADVCLFTTLI 306


>At1g02940.1 68414.m00261 glutathione S-transferase, putative
           similar to glutathione S-transferase GI:860955 from
           [Hyoscyamus muticus]
          Length = 256

 Score = 34.3 bits (75), Expect = 0.089
 Identities = 20/48 (41%), Positives = 27/48 (56%)
 Frame = +1

Query: 7   VAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA 150
           +  N + G+  K   FL   P G+VP F   DG + LTES AI+ Y+A
Sbjct: 67  ITVNLIAGD-QKKPSFLAINPFGQVPVF--LDGGLKLTESRAISEYIA 111


>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
           EF-1-beta, putative nearly identical to eEF-1beta
           [Arabidopsis thaliana] GI:398606
          Length = 231

 Score = 33.5 bits (73), Expect = 0.15
 Identities = 19/55 (34%), Positives = 28/55 (50%)
 Frame = +3

Query: 318 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWF 482
           + LK LD HLLTR+++   + +  D+ VF+         L     S  +NV RWF
Sbjct: 11  SGLKKLDEHLLTRSYITGYQASKDDITVFT--------ALSKPPTSEFVNVSRWF 57


>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
           identical to SP|P48006 Elongation factor 1-beta
           (EF-1-beta) {Arabidopsis thaliana}
          Length = 231

 Score = 31.9 bits (69), Expect = 0.47
 Identities = 19/55 (34%), Positives = 29/55 (52%)
 Frame = +3

Query: 318 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWF 482
           A LK LD HLLTR+++   + +  D+ VF+ L         P+  S  +N  RW+
Sbjct: 11  AGLKKLDEHLLTRSYITGYQASKDDITVFAAL------AKPPT--SQYVNASRWY 57


>At3g25670.1 68416.m03195 leucine-rich repeat family protein
           contains leucine rich-repeat (LRR) domains Pfam:PF00560,
           INTERPRO:IPR001611; +
          Length = 475

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = +1

Query: 208 SWSDSELLPASCAWVFLTLVSCNSTNRMLNVQSLT 312
           SW+ S+L P  C W  +  VSC+    +  V  LT
Sbjct: 52  SWNGSDLYPDPCGWTPIQGVSCDLYGDLWYVTDLT 86


>At3g14120.2 68416.m01786 expressed protein similar to Nuclear pore
           complex protein Nup107 (Nucleoporin Nup107) (107 kDa
           nucleoporin) (p105) (Swiss-Prot:P52590) [Rattus
           norvegicus]
          Length = 1077

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 6/60 (10%)
 Frame = +1

Query: 184 QARVWQWASWSDSELLPASCAW---VFLTLVSCNSTNRMLNV---QSLTYWPP*KYWTDI 345
           Q R+W+WAS+  SE +         V +    C++ NRML +        W   K W D+
Sbjct: 387 QLRLWKWASYCASEKIAEQDGGKHEVAVFATQCSNLNRMLPICTDWESACWAMAKSWLDV 446


>At3g14120.1 68416.m01785 expressed protein similar to Nuclear pore
           complex protein Nup107 (Nucleoporin Nup107) (107 kDa
           nucleoporin) (p105) (Swiss-Prot:P52590) [Rattus
           norvegicus]
          Length = 1101

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 6/60 (10%)
 Frame = +1

Query: 184 QARVWQWASWSDSELLPASCAW---VFLTLVSCNSTNRMLNV---QSLTYWPP*KYWTDI 345
           Q R+W+WAS+  SE +         V +    C++ NRML +        W   K W D+
Sbjct: 387 QLRLWKWASYCASEKIAEQDGGKHEVAVFATQCSNLNRMLPICTDWESACWAMAKSWLDV 446


>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
           domain-containing protein-related contains weak hit to
           Pfam F00627: UBA/TS-N domain; supported by tandem
           duplication of ubiquitin-associated (UBA)/TS-N domain
           protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
           thaliana]
          Length = 508

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 12/40 (30%), Positives = 19/40 (47%)
 Frame = +1

Query: 262 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRES 381
           L++ +  N   N +S + WPP K W D   H   +   E+
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEA 234


>At1g13230.1 68414.m01535 leucine-rich repeat family protein
           contains leucine rich-repeat (LRR) domains Pfam:PF00560,
           INTERPRO:IPR001611; contains similarity to gb|U42445
           Cf-2.2 from Lycopersicon pimpinellifolium
          Length = 424

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = +1

Query: 208 SWSDSELLPASCAWVFLTLVSCNSTNRMLNVQSLT 312
           SW+ S L P  C W  +  VSC+  N +  V  L+
Sbjct: 55  SWNGSALYPDPCGWTPIQGVSCDIYNDLWYVTDLS 89


>At5g07270.1 68418.m00829 ankyrin repeat family protein contains
           ankyrin repeats, Pfam:PF00023
          Length = 513

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 20/50 (40%), Positives = 23/50 (46%), Gaps = 7/50 (14%)
 Frame = +1

Query: 295 NVQSLTYWPP*-------KYWTDIFSHAPSLLPRESHLPMSLSSVHCCML 423
           NV S+T  PP        ++    F   PS L RE  LPMSL     CML
Sbjct: 344 NVPSVTVGPPGSIPCPLCRHGITAFKRLPSSLTREMKLPMSLGFCAPCML 393


>At1g79280.1 68414.m09242 expressed protein weak similarity to
            Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens]
          Length = 2111

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 13/38 (34%), Positives = 18/38 (47%)
 Frame = +2

Query: 482  PDRRPPATSSAVVGSLTLCAAPPTYDPKKYQELAGAQN 595
            P    P TSS +  ++T   AP T DPK+     G  +
Sbjct: 2009 PVSTAPTTSSTLASAITSSGAPETEDPKRAPSPGGGSS 2046


>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
           long-chain acyl-CoA synthetase nearly identical to acyl
           CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
          Length = 665

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 13/44 (29%), Positives = 22/44 (50%)
 Frame = -3

Query: 466 FISDERTLGSSTCWKACSSVLKTMTSASVILSVTRKVRVRRCPS 335
           F+ ++      +C K CSS LKT+ S   + S  ++    +C S
Sbjct: 156 FVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVS 199


>At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative /
           glutamyl-tRNA synthetase, putatuve / GluRS, putative
           identical to gi:3435196
          Length = 719

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 15/53 (28%), Positives = 27/53 (50%)
 Frame = +3

Query: 333 LDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTV 491
           +D +L + TFLV   +++ADV ++S L    Q            ++ RWF ++
Sbjct: 104 VDKYLESSTFLVGHSLSIADVAIWSALAGTGQRWESLRKSKKYQSLVRWFNSI 156


>At3g28910.1 68416.m03608 myb family transcription factor (MYB30)
           identical to myb-like protein GB:AJ007289 [Arabidopsis
           thaliana] (Plant J. 20 (1), 57-66 (1999))
          Length = 323

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
 Frame = -2

Query: 719 RRGPWTP*AFQAPPLQGQEQLPPSSWVALSFHTSYLFCPPSCSV---HQLTPG 570
           ++GPWTP          QE   P +W A+  +T  L C  SC +   + L PG
Sbjct: 13  KKGPWTPEEDIILVTYIQEH-GPGNWRAVPTNTGLLRCSKSCRLRWTNYLRPG 64


>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
           (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
           S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
           (Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
           {Arabidopsis thaliana}; contains Pfam profiles PF02798:
           Glutathione S-transferase, N-terminal domain and
           PF00043:Glutathione S-transferase, C-terminal domain
          Length = 228

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 15/34 (44%), Positives = 20/34 (58%)
 Frame = +1

Query: 46  EDFLKKFPAGKVPAFESADGKVLLTESNAIAYYV 147
           +DF K  P G VPA    DG V++ +S AI  Y+
Sbjct: 56  QDFKKINPMGTVPAL--VDGDVVINDSFAIIMYL 87


>At1g68780.1 68414.m07862 leucine-rich repeat family protein
           contains leucine rich-repeat (LRR) domains Pfam:PF00560,
           INTERPRO:IPR001611; contains similarity to disease
           resistance protein [Lycopersicon esculentum]
           gi|3894383|gb|AAC78591
          Length = 432

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 11/36 (30%), Positives = 20/36 (55%)
 Frame = +1

Query: 208 SWSDSELLPASCAWVFLTLVSCNSTNRMLNVQSLTY 315
           SW+ S L P  C W  +  V+C+  + +  V +L++
Sbjct: 57  SWNGSYLYPDPCGWTPIQGVTCDIYDELWYVTALSF 92


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,229,910
Number of Sequences: 28952
Number of extensions: 356309
Number of successful extensions: 1010
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 979
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1009
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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