BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20983 (756 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 61 7e-10 At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 60 2e-09 At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 42 4e-04 At4g02520.1 68417.m00345 glutathione S-transferase, putative 39 0.003 At2g02930.1 68415.m00241 glutathione S-transferase, putative 39 0.003 At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 38 0.005 At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 38 0.005 At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 38 0.007 At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 36 0.022 At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 34 0.089 At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 33 0.15 At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 32 0.47 At3g25670.1 68416.m03195 leucine-rich repeat family protein cont... 29 2.5 At3g14120.2 68416.m01786 expressed protein similar to Nuclear po... 29 2.5 At3g14120.1 68416.m01785 expressed protein similar to Nuclear po... 29 2.5 At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 29 2.5 At1g13230.1 68414.m01535 leucine-rich repeat family protein cont... 29 3.3 At5g07270.1 68418.m00829 ankyrin repeat family protein contains ... 29 4.4 At1g79280.1 68414.m09242 expressed protein weak similarity to Nu... 29 4.4 At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 29 4.4 At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / gluta... 28 7.7 At3g28910.1 68416.m03608 myb family transcription factor (MYB30)... 28 7.7 At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 28 7.7 At1g68780.1 68414.m07862 leucine-rich repeat family protein cont... 28 7.7 >At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / eEF-1B gamma, putative Similar to elongation factor 1-gamma (gb|EF1G_XENLA). ESTs gb|T20564,gb|T45940,gb|T04527 come from this gene Length = 414 Score = 61.3 bits (142), Expect = 7e-10 Identities = 35/87 (40%), Positives = 47/87 (54%), Gaps = 4/87 (4%) Frame = +1 Query: 1 VKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA----NESLRG 168 + V +F G TNK+ FLK P GKVP E+ +G V ESNAIA YV+ + SL G Sbjct: 28 IDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETPEGSVF--ESNAIARYVSRLNGDNSLNG 85 Query: 169 GDLATQARVWQWASWSDSELLPASCAW 249 L A++ QW +S E+ + W Sbjct: 86 SSLIEYAQIEQWIDFSSLEIYASILRW 112 Score = 51.6 bits (118), Expect = 5e-07 Identities = 30/81 (37%), Positives = 42/81 (51%) Frame = +3 Query: 255 PYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHV 434 P +G M ++ E A S L AL L+ HL + T+LV ITLAD+I L F V Sbjct: 115 PRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATV 174 Query: 435 LDPSVRSSLINVQRWFLTVAH 497 + S +V+R+F TV + Sbjct: 175 MTKKFTSEFPHVERYFWTVVN 195 >At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / eEF-1B gamma, putative similar to elongation factor 1B gamma GI:3868758 from [Oryza sativa] Length = 413 Score = 60.1 bits (139), Expect = 2e-09 Identities = 34/87 (39%), Positives = 47/87 (54%), Gaps = 4/87 (4%) Frame = +1 Query: 1 VKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA----NESLRG 168 ++ + +F G TNKS +FLK P GKVP E+ +G + ESNAIA YV+ + SL G Sbjct: 28 IEESADFQMGVTNKSPEFLKMNPIGKVPVLETPEGPIF--ESNAIARYVSRKNGDNSLNG 85 Query: 169 GDLATQARVWQWASWSDSELLPASCAW 249 L A + QW +S E+ W Sbjct: 86 SSLIEYAHIEQWIDFSSLEIDANMLKW 112 Score = 49.2 bits (112), Expect = 3e-06 Identities = 27/81 (33%), Positives = 42/81 (51%) Frame = +3 Query: 255 PYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHV 434 P +G F+ E A S L L+ L+ HL + TFLV +TLAD++ L F V Sbjct: 115 PRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATV 174 Query: 435 LDPSVRSSLINVQRWFLTVAH 497 + S+ +V+R+F T+ + Sbjct: 175 MTKKFTSAFPHVERYFWTMVN 195 >At1g02930.1 68414.m00260 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 208 Score = 41.9 bits (94), Expect = 4e-04 Identities = 23/53 (43%), Positives = 33/53 (62%), Gaps = 1/53 (1%) Frame = +1 Query: 37 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVANE-SLRGGDLATQAR 192 +K E F+ + P GKVPAFE D K+ ES AI Y+A+E S +G +L + + Sbjct: 41 HKKEPFILRNPFGKVPAFEDGDFKIF--ESRAITQYIAHEFSDKGNNLLSTGK 91 >At4g02520.1 68417.m00345 glutathione S-transferase, putative Length = 212 Score = 39.1 bits (87), Expect = 0.003 Identities = 20/39 (51%), Positives = 26/39 (66%) Frame = +1 Query: 37 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 153 +K E FL + P G+VPAFE DG + L ES AI Y+A+ Sbjct: 41 HKKEPFLSRNPFGQVPAFE--DGDLKLFESRAITQYIAH 77 >At2g02930.1 68415.m00241 glutathione S-transferase, putative Length = 212 Score = 39.1 bits (87), Expect = 0.003 Identities = 20/39 (51%), Positives = 26/39 (66%) Frame = +1 Query: 37 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 153 +K E FL + P G+VPAFE DG + L ES AI Y+A+ Sbjct: 41 HKKEPFLSRNPFGQVPAFE--DGDLKLFESRAITQYIAH 77 >At1g02950.2 68414.m00263 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 245 Score = 38.3 bits (85), Expect = 0.005 Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%) Frame = +1 Query: 37 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 177 +K+E FL P G+VP FE DG V L ES AI Y+A S RG L Sbjct: 64 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 109 >At1g02950.1 68414.m00262 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 243 Score = 38.3 bits (85), Expect = 0.005 Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%) Frame = +1 Query: 37 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 177 +K+E FL P G+VP FE DG V L ES AI Y+A S RG L Sbjct: 62 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 107 >At1g02920.1 68414.m00259 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus]; supported by cDNA GI:443697. Length = 209 Score = 37.9 bits (84), Expect = 0.007 Identities = 20/39 (51%), Positives = 25/39 (64%) Frame = +1 Query: 37 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 153 +K E F+ + P GKVPAFE DG L ES AI Y+A+ Sbjct: 41 HKKEPFIFRNPFGKVPAFE--DGDFKLFESRAITQYIAH 77 >At5g44000.1 68418.m05384 glutathione S-transferase C-terminal domain-containing protein contains Pfam domain PF00043: Glutathione S-transferase, C-terminal domain Length = 399 Score = 36.3 bits (80), Expect = 0.022 Identities = 16/46 (34%), Positives = 31/46 (67%) Frame = +3 Query: 279 NKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLL 416 +++ + A ++L + L ++ HL + +L ER+TLADV +F+TL+ Sbjct: 261 SQEAYDGAVNELFSTLDEIEDHLGSNRYLCGERLTLADVCLFTTLI 306 >At1g02940.1 68414.m00261 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 256 Score = 34.3 bits (75), Expect = 0.089 Identities = 20/48 (41%), Positives = 27/48 (56%) Frame = +1 Query: 7 VAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA 150 + N + G+ K FL P G+VP F DG + LTES AI+ Y+A Sbjct: 67 ITVNLIAGD-QKKPSFLAINPFGQVPVF--LDGGLKLTESRAISEYIA 111 >At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF-1-beta, putative nearly identical to eEF-1beta [Arabidopsis thaliana] GI:398606 Length = 231 Score = 33.5 bits (73), Expect = 0.15 Identities = 19/55 (34%), Positives = 28/55 (50%) Frame = +3 Query: 318 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWF 482 + LK LD HLLTR+++ + + D+ VF+ L S +NV RWF Sbjct: 11 SGLKKLDEHLLTRSYITGYQASKDDITVFT--------ALSKPPTSEFVNVSRWF 57 >At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta identical to SP|P48006 Elongation factor 1-beta (EF-1-beta) {Arabidopsis thaliana} Length = 231 Score = 31.9 bits (69), Expect = 0.47 Identities = 19/55 (34%), Positives = 29/55 (52%) Frame = +3 Query: 318 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWF 482 A LK LD HLLTR+++ + + D+ VF+ L P+ S +N RW+ Sbjct: 11 AGLKKLDEHLLTRSYITGYQASKDDITVFAAL------AKPPT--SQYVNASRWY 57 >At3g25670.1 68416.m03195 leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam:PF00560, INTERPRO:IPR001611; + Length = 475 Score = 29.5 bits (63), Expect = 2.5 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +1 Query: 208 SWSDSELLPASCAWVFLTLVSCNSTNRMLNVQSLT 312 SW+ S+L P C W + VSC+ + V LT Sbjct: 52 SWNGSDLYPDPCGWTPIQGVSCDLYGDLWYVTDLT 86 >At3g14120.2 68416.m01786 expressed protein similar to Nuclear pore complex protein Nup107 (Nucleoporin Nup107) (107 kDa nucleoporin) (p105) (Swiss-Prot:P52590) [Rattus norvegicus] Length = 1077 Score = 29.5 bits (63), Expect = 2.5 Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 6/60 (10%) Frame = +1 Query: 184 QARVWQWASWSDSELLPASCAW---VFLTLVSCNSTNRMLNV---QSLTYWPP*KYWTDI 345 Q R+W+WAS+ SE + V + C++ NRML + W K W D+ Sbjct: 387 QLRLWKWASYCASEKIAEQDGGKHEVAVFATQCSNLNRMLPICTDWESACWAMAKSWLDV 446 >At3g14120.1 68416.m01785 expressed protein similar to Nuclear pore complex protein Nup107 (Nucleoporin Nup107) (107 kDa nucleoporin) (p105) (Swiss-Prot:P52590) [Rattus norvegicus] Length = 1101 Score = 29.5 bits (63), Expect = 2.5 Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 6/60 (10%) Frame = +1 Query: 184 QARVWQWASWSDSELLPASCAW---VFLTLVSCNSTNRMLNV---QSLTYWPP*KYWTDI 345 Q R+W+WAS+ SE + V + C++ NRML + W K W D+ Sbjct: 387 QLRLWKWASYCASEKIAEQDGGKHEVAVFATQCSNLNRMLPICTDWESACWAMAKSWLDV 446 >At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-containing protein-related contains weak hit to Pfam F00627: UBA/TS-N domain; supported by tandem duplication of ubiquitin-associated (UBA)/TS-N domain protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis thaliana] Length = 508 Score = 29.5 bits (63), Expect = 2.5 Identities = 12/40 (30%), Positives = 19/40 (47%) Frame = +1 Query: 262 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRES 381 L++ + N N +S + WPP K W D H + E+ Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEA 234 >At1g13230.1 68414.m01535 leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam:PF00560, INTERPRO:IPR001611; contains similarity to gb|U42445 Cf-2.2 from Lycopersicon pimpinellifolium Length = 424 Score = 29.1 bits (62), Expect = 3.3 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +1 Query: 208 SWSDSELLPASCAWVFLTLVSCNSTNRMLNVQSLT 312 SW+ S L P C W + VSC+ N + V L+ Sbjct: 55 SWNGSALYPDPCGWTPIQGVSCDIYNDLWYVTDLS 89 >At5g07270.1 68418.m00829 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 513 Score = 28.7 bits (61), Expect = 4.4 Identities = 20/50 (40%), Positives = 23/50 (46%), Gaps = 7/50 (14%) Frame = +1 Query: 295 NVQSLTYWPP*-------KYWTDIFSHAPSLLPRESHLPMSLSSVHCCML 423 NV S+T PP ++ F PS L RE LPMSL CML Sbjct: 344 NVPSVTVGPPGSIPCPLCRHGITAFKRLPSSLTREMKLPMSLGFCAPCML 393 >At1g79280.1 68414.m09242 expressed protein weak similarity to Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens] Length = 2111 Score = 28.7 bits (61), Expect = 4.4 Identities = 13/38 (34%), Positives = 18/38 (47%) Frame = +2 Query: 482 PDRRPPATSSAVVGSLTLCAAPPTYDPKKYQELAGAQN 595 P P TSS + ++T AP T DPK+ G + Sbjct: 2009 PVSTAPTTSSTLASAITSSGAPETEDPKRAPSPGGGSS 2046 >At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / long-chain acyl-CoA synthetase nearly identical to acyl CoA synthetase (MF45P) GI:1617268 from [Brassica napus] Length = 665 Score = 28.7 bits (61), Expect = 4.4 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = -3 Query: 466 FISDERTLGSSTCWKACSSVLKTMTSASVILSVTRKVRVRRCPS 335 F+ ++ +C K CSS LKT+ S + S ++ +C S Sbjct: 156 FVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVS 199 >At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / glutamyl-tRNA synthetase, putatuve / GluRS, putative identical to gi:3435196 Length = 719 Score = 27.9 bits (59), Expect = 7.7 Identities = 15/53 (28%), Positives = 27/53 (50%) Frame = +3 Query: 333 LDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTV 491 +D +L + TFLV +++ADV ++S L Q ++ RWF ++ Sbjct: 104 VDKYLESSTFLVGHSLSIADVAIWSALAGTGQRWESLRKSKKYQSLVRWFNSI 156 >At3g28910.1 68416.m03608 myb family transcription factor (MYB30) identical to myb-like protein GB:AJ007289 [Arabidopsis thaliana] (Plant J. 20 (1), 57-66 (1999)) Length = 323 Score = 27.9 bits (59), Expect = 7.7 Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 3/53 (5%) Frame = -2 Query: 719 RRGPWTP*AFQAPPLQGQEQLPPSSWVALSFHTSYLFCPPSCSV---HQLTPG 570 ++GPWTP QE P +W A+ +T L C SC + + L PG Sbjct: 13 KKGPWTPEEDIILVTYIQEH-GPGNWRAVPTNTGLLRCSKSCRLRWTNYLRPG 64 >At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 228 Score = 27.9 bits (59), Expect = 7.7 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = +1 Query: 46 EDFLKKFPAGKVPAFESADGKVLLTESNAIAYYV 147 +DF K P G VPA DG V++ +S AI Y+ Sbjct: 56 QDFKKINPMGTVPAL--VDGDVVINDSFAIIMYL 87 >At1g68780.1 68414.m07862 leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam:PF00560, INTERPRO:IPR001611; contains similarity to disease resistance protein [Lycopersicon esculentum] gi|3894383|gb|AAC78591 Length = 432 Score = 27.9 bits (59), Expect = 7.7 Identities = 11/36 (30%), Positives = 20/36 (55%) Frame = +1 Query: 208 SWSDSELLPASCAWVFLTLVSCNSTNRMLNVQSLTY 315 SW+ S L P C W + V+C+ + + V +L++ Sbjct: 57 SWNGSYLYPDPCGWTPIQGVTCDIYDELWYVTALSF 92 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,229,910 Number of Sequences: 28952 Number of extensions: 356309 Number of successful extensions: 1010 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 979 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1009 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1682736544 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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