Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20975
(734 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 159 3e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 156 3e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 101 1e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 4e-14
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 29 0.52
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.6
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 26 4.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.4
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 6.4
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.5
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 159 bits (387), Expect = 3e-40
Identities = 68/100 (68%), Positives = 82/100 (82%)
Frame = +3
Query: 222 FQHFLQRDRSWKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 401
F F K+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 402 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGPALG 521
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGG G
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSG 148
Score = 129 bits (312), Expect = 4e-31
Identities = 56/75 (74%), Positives = 69/75 (92%)
Frame = +2
Query: 509 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV 688
TGSGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMV
Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMV 204
Query: 689 DNEAIYDICRRNLDI 733
DNE+ YDICRRNLDI
Sbjct: 205 DNESCYDICRRNLDI 219
Score = 96.7 bits (230), Expect = 3e-21
Identities = 42/63 (66%), Positives = 46/63 (73%)
Frame = +1
Query: 79 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 258
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG G
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 259 TYP 267
P
Sbjct: 61 YVP 63
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 156 bits (379), Expect = 3e-39
Identities = 65/100 (65%), Positives = 82/100 (82%)
Frame = +3
Query: 222 FQHFLQRDRSWKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 401
F F K VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 402 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGPALG 521
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGGG G
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSG 152
Score = 132 bits (319), Expect = 5e-32
Identities = 57/75 (76%), Positives = 69/75 (92%)
Frame = +2
Query: 509 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV 688
TGSG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMV
Sbjct: 149 TGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMV 208
Query: 689 DNEAIYDICRRNLDI 733
DNEA YDICRRNLDI
Sbjct: 209 DNEACYDICRRNLDI 223
Score = 90.6 bits (215), Expect = 2e-19
Identities = 46/68 (67%), Positives = 48/68 (70%), Gaps = 5/68 (7%)
Frame = +1
Query: 79 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 243
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 244 TGAGSTYP 267
TG G P
Sbjct: 60 TGQGKFVP 67
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 101 bits (241), Expect = 1e-22
Identities = 45/89 (50%), Positives = 60/89 (67%)
Frame = +3
Query: 255 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVL 434
K+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VL
Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117
Query: 435 DRIRKLADQCTGLQGFLIFHSFGGGPALG 521
D +R+ A+ C LQGF + HS GGG G
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSG 146
Score = 66.9 bits (156), Expect = 3e-12
Identities = 31/75 (41%), Positives = 46/75 (61%)
Frame = +2
Query: 509 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV 688
TGSG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +
Sbjct: 143 TGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCI 202
Query: 689 DNEAIYDICRRNLDI 733
DNEA+ I L I
Sbjct: 203 DNEALSSIFANTLKI 217
Score = 38.7 bits (86), Expect = 8e-04
Identities = 20/63 (31%), Positives = 27/63 (42%)
Frame = +1
Query: 79 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 258
MRE + + GQ G Q+G A W EHG+ G T + N +F+E G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 259 TYP 267
P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 72.9 bits (171), Expect = 4e-14
Identities = 37/99 (37%), Positives = 59/99 (59%), Gaps = 2/99 (2%)
Frame = +3
Query: 231 FLQRDRSWKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 404
F Q D + +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 405 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGPALG 521
+ I + ++D I + AD L+GF + HS GG G
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSG 148
Score = 52.4 bits (120), Expect = 6e-08
Identities = 29/74 (39%), Positives = 42/74 (56%), Gaps = 1/74 (1%)
Frame = +2
Query: 509 TGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFM 685
TGSG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D +
Sbjct: 145 TGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVV 204
Query: 686 VDNEAIYDICRRNL 727
+DN A+ I L
Sbjct: 205 LDNAALAHIAADRL 218
Score = 48.8 bits (111), Expect = 8e-07
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +1
Query: 82 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 246
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 29.5 bits (63), Expect = 0.52
Identities = 15/46 (32%), Positives = 26/46 (56%)
Frame = -1
Query: 566 SCRSQRRDAPSIRK*TQSRSSTEGVEDQESLQTGTLVSELADSVQN 429
S + SIR ++SRSS+ G + Q S+Q+GT ++D ++
Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.9 bits (59), Expect = 1.6
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -1
Query: 314 ADLINNSRFKIDEDS--TGYVLPAPVSLKKVLKESSP 210
AD+IN SR+ ID S T + P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 26.2 bits (55), Expect = 4.8
Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = -1
Query: 347 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIW 180
F + + S +++N+ + KID GY S K + E + P + G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 177 WPDAHRQDHRGWRRFFQHFLQR 242
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 6.4
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -1
Query: 281 DEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIWPSG 171
++D T Y+ P P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKYLGP-PIYTSQRLYDTTPPGVVMGLGWTPMG 869
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.5
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 68 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 190
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,077,830
Number of Sequences: 5004
Number of extensions: 65967
Number of successful extensions: 202
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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