Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20971
(711 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyce... 95 9e-21
SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy... 58 9e-10
SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2 ... 40 4e-04
SPBC17A3.03c |||phosphoprotein phosphatase |Schizosaccharomyces ... 27 3.5
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 26 4.6
SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosa... 26 4.6
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 25 8.1
SPAC343.01c |erg8||phosphomevalonate kinase |Schizosaccharomyces... 25 8.1
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 8.1
>SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 285
Score = 95.1 bits (226), Expect = 9e-21
Identities = 48/81 (59%), Positives = 57/81 (70%)
Frame = +2
Query: 266 IVAVDLQAMAALPGVKQIQGDITKQETANAIIEEFQGLKADLVVCDGAPDVTGLHDIDEY 445
IVAVDLQ MA + GV +Q DIT T + I+ F ADLVV DGAPDVTGLHD+DEY
Sbjct: 79 IVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPADLVVSDGAPDVTGLHDLDEY 138
Query: 446 VQSQLLLAALNITTHVLKNEG 508
+Q+Q+LLAA N+ VLK G
Sbjct: 139 IQAQILLAAFNLAVCVLKPGG 159
Score = 90.2 bits (214), Expect = 3e-19
Identities = 38/50 (76%), Positives = 45/50 (90%)
Frame = +1
Query: 46 MGKTSKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFNGVLRAVDLCA 195
MG++SKDKRD YYRLAKE+GWRARSAFKLLQ+NE++N+F G R VDLCA
Sbjct: 1 MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCA 50
Score = 89.8 bits (213), Expect = 3e-19
Identities = 39/61 (63%), Positives = 51/61 (83%)
Frame = +1
Query: 511 FVAKIFRGKDVSLLYSQLKQFFKLVTVSKPRSSRNSSIEAFVICEKYNPPEDYVPNMVNP 690
FVAKIFRG+DVSLLYSQL+ F+ V+ +KPRSSR SSIE+FV+CE +NPP ++ P++ P
Sbjct: 161 FVAKIFRGRDVSLLYSQLRLMFRKVSCAKPRSSRASSIESFVVCEDFNPPSNFQPDLTKP 220
Query: 691 L 693
L
Sbjct: 221 L 221
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3
Query: 198 PGSWSQVLTKNLRQN 242
PGSWSQVL++ L +N
Sbjct: 52 PGSWSQVLSRELLKN 66
>SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 802
Score = 58.4 bits (135), Expect = 9e-10
Identities = 24/52 (46%), Positives = 39/52 (75%)
Frame = +1
Query: 40 KNMGKTSKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFNGVLRAVDLCA 195
K+ KT+K + D +Y+LAKE+G+R+R+AFKL+Q+N++Y+ +DLCA
Sbjct: 3 KSQKKTAKGRLDKWYKLAKEQGYRSRAAFKLVQLNQKYSFLEKAKVIIDLCA 54
Score = 56.0 bits (129), Expect = 5e-09
Identities = 26/61 (42%), Positives = 34/61 (55%)
Frame = +1
Query: 508 SFVAKIFRGKDVSLLYSQLKQFFKLVTVSKPRSSRNSSIEAFVICEKYNPPEDYVPNMVN 687
+FV K+FR +D + L KQ F V +KP SSRN S E FV+C Y P+ P +
Sbjct: 154 TFVTKVFRSRDYNNLLWVFKQLFNKVEATKPPSSRNVSAEIFVVCRGYKAPKKLDPRFTD 213
Query: 688 P 690
P
Sbjct: 214 P 214
Score = 44.0 bits (99), Expect = 2e-05
Identities = 28/88 (31%), Positives = 45/88 (51%)
Frame = +2
Query: 233 STKCCKY*DVKIVAVDLQAMAALPGVKQIQGDITKQETANAIIEEFQGLKADLVVCDGAP 412
++K CK + IV VDL + +P DIT + + + + KAD+V+ DGAP
Sbjct: 63 ASKTCKPGSL-IVGVDLAPIKPIPNCHTFVEDITSDKCRSQLRGYLKTWKADVVLHDGAP 121
Query: 413 DVTGLHDIDEYVQSQLLLAALNITTHVL 496
+V D Y Q+QL+L ++ + L
Sbjct: 122 NVGSAWLQDAYGQAQLVLMSMKLACEFL 149
>SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 218
Score = 39.9 bits (89), Expect = 4e-04
Identities = 18/50 (36%), Positives = 29/50 (58%)
Frame = +1
Query: 508 SFVAKIFRGKDVSLLYSQLKQFFKLVTVSKPRSSRNSSIEAFVICEKYNP 657
SF+ K + G + + L + LK F+ V V KP++S S EA+ +C + P
Sbjct: 169 SFICKFYMGDEDADLQNLLKSHFRFVQVMKPKASLKESREAYFVCLERKP 218
Score = 35.1 bits (77), Expect = 0.010
Identities = 12/36 (33%), Positives = 25/36 (69%)
Frame = +1
Query: 58 SKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFN 165
+K +D Y + +K + +R+R+A+KL+++N +Y N
Sbjct: 12 AKRSKDFYRKKSKIDNFRSRAAYKLIELNSKYRFIN 47
Score = 34.3 bits (75), Expect = 0.017
Identities = 26/100 (26%), Positives = 42/100 (42%), Gaps = 12/100 (12%)
Frame = +2
Query: 263 KIVAVDLQAMAALPGVKQIQGDITKQETANAIIEEFQGLK------------ADLVVCDG 406
K++ +D+Q +A GV I GDI T + E + L D V+ D
Sbjct: 75 KVIGIDIQHIAPPEGVLPIYGDIRDPNTLTKLFEALRLLHEPNTNDSIDCRVVDAVISDM 134
Query: 407 APDVTGLHDIDEYVQSQLLLAALNITTHVLKNEGHLWPKY 526
TG+ D + +L +AL++ LK+ G K+
Sbjct: 135 LHKATGIRIRDHALSMELCASALHVALTFLKSNGSFICKF 174
>SPBC17A3.03c |||phosphoprotein phosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 263
Score = 26.6 bits (56), Expect = 3.5
Identities = 18/45 (40%), Positives = 23/45 (51%)
Frame = -2
Query: 677 FGT*SSGGLYFSQITKASMLEFLELLGLDTVTSLKNCFNCEYSRE 543
FG G +Y S +AS FLE L + T+ SL+ EYS E
Sbjct: 62 FGVVYPGIIYRSACPRASNFNFLESLHIRTIISLR---QEEYSEE 103
>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
Dna2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1398
Score = 26.2 bits (55), Expect = 4.6
Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = -2
Query: 617 EFLELLGLDTVTSLKNCFNCE-YSRETSFPLNILATNDLHS*AHGLLCSMQRV-ITEIGH 444
EFL+ L D +T+LK C E YS P T + S LL +++ +T H
Sbjct: 928 EFLKCLNEDQITALKKCHAAEHYSLILGMP-GTGKTTTISSLIRSLLAKKKKILLTSFTH 986
Query: 443 I 441
+
Sbjct: 987 L 987
>SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = -1
Query: 351 FAVSCLVISP*ICFTPGNAAIA*RSTATILTS*YLQHFVEGSW 223
+ V ++I P F+PG + +S + Y+QHF GSW
Sbjct: 353 YLVGDVLILPITAFSPGVGHMGSKSPNDPMA--YVQHFFAGSW 393
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 25.4 bits (53), Expect = 8.1
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +2
Query: 317 IQGDITKQETANAIIEEFQGLKADLVVCDGAPDVTGLHDIDEYVQSQLLLAAL 475
+ GD+ +ETA + Q + D V D P + L ++ QS+ L+AL
Sbjct: 1996 MDGDLEVRETAAEAFDSLQSILGDRAVDDVLPQLLKL--LESENQSEQALSAL 2046
>SPAC343.01c |erg8||phosphomevalonate kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 426
Score = 25.4 bits (53), Expect = 8.1
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -2
Query: 575 KNCFNCEYSRETSFPLNILATNDLHS*AHGLLCSMQRVITEI 450
KNCF+ YSR S L++ L S S++R++ I
Sbjct: 307 KNCFDDLYSRVLSIKNCFLSSESLDSELQSQFRSIRRILQRI 348
>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 791
Score = 25.4 bits (53), Expect = 8.1
Identities = 22/80 (27%), Positives = 37/80 (46%), Gaps = 1/80 (1%)
Frame = +2
Query: 311 KQIQGDITKQETANAIIEEFQGLKADLVVCDGAPDVTGLHDIDEYVQSQLLLAALNITTH 490
K+I+G +T N I E +GLK D D +G+H + V++ A N
Sbjct: 190 KEIEGTVTN----NIHIAEERGLKVDDSGLDEEDLYSGVHRSIDVVRNYTRSNAYNKNNK 245
Query: 491 VLKNEGHLWP-KYSEEKMFP 547
K + H P ++ ++K+ P
Sbjct: 246 DQKPKNHEAPHQHPQQKVVP 265
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,093,301
Number of Sequences: 5004
Number of extensions: 65543
Number of successful extensions: 182
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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