Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20954
(669 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_10518| Best HMM Match : No HMM Matches (HMM E-Value=.) 121 5e-28
SB_31500| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.64
SB_29237| Best HMM Match : I-set (HMM E-Value=0) 29 3.4
SB_38076| Best HMM Match : 7tm_1 (HMM E-Value=1.3e-05) 29 4.5
SB_3148| Best HMM Match : Keratin_B2 (HMM E-Value=0.26) 28 6.0
SB_50551| Best HMM Match : Extensin_2 (HMM E-Value=0.41) 24 7.0
SB_7532| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.9
>SB_10518| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 328
Score = 121 bits (292), Expect = 5e-28
Identities = 64/143 (44%), Positives = 96/143 (67%), Gaps = 3/143 (2%)
Frame = +3
Query: 249 GRKSRGILQRLYGKHIISVANEIGRLPPTFLDASQLATAILTSGYEFGSGKIIYNKFKSV 428
G K++ IL R GK+++ ++G+ PP F +A+ LA IL +G++F +G + YN F+SV
Sbjct: 163 GDKTKQILSRTLGKNMLMSFMDVGKKPPLFCEATFLAQEILDAGFDFNTGDMFYNVFRSV 222
Query: 429 VSYAQSDLAPLH*EVY*ERQQADGIRL---LDSDVLQSYTEFSLASLLFYALKEGACSEQ 599
VS+ S + + +D + LDS+V++ Y EF+LAS+LF+ +KE +CSE
Sbjct: 223 VSFRASTKSIYSFDNL-NNAASDSMSSYDELDSEVIRCYQEFNLASMLFFGMKEQSCSEH 281
Query: 600 SSRMTAMDNASKSAGEMIDKLTL 668
S+RMTAMD A+K+AGEMIDKLTL
Sbjct: 282 SARMTAMDAATKNAGEMIDKLTL 304
Score = 76.2 bits (179), Expect = 2e-14
Identities = 32/56 (57%), Positives = 43/56 (76%)
Frame = +2
Query: 2 KMVSAAKYTRAERDLKAARPYGEGAVQFYERAEVTPPEDDPKQLFVAMTSDRGLCG 169
KMVSAAK+ RAE++LK+AR YG+GA Y++ E+ +DPK L V ++SDRGLCG
Sbjct: 80 KMVSAAKFGRAEKELKSARAYGDGATALYDKVEIKQESEDPKHLIVVLSSDRGLCG 135
>SB_31500| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1446
Score = 31.5 bits (68), Expect = 0.64
Identities = 32/129 (24%), Positives = 57/129 (44%)
Frame = +1
Query: 271 CRDCTESTSLVLLMRLDVSHLLSWTQVSWPLPFSPQDTSLVPERSFITSSSLWYRTPSPT 450
C++ +S L +HL + +Q++ PLP P TSS T +P
Sbjct: 962 CQETPTDSSTASLAHTSPAHLATASQITHPLPHRTISIP-YPSPPHTTSSQSSQATHTP- 1019
Query: 451 WPLYTKKSIESASKLTAYDFWTATYSNPTRSSRWPRCCSTR*RRALAQSSRPA*PPWTTP 630
++++ + + +A YS+P+R+S P T A SS+P+ PP+++
Sbjct: 1020 ----PPRAVDLPHPSSPHSS-SALYSSPSRTSNLPYTTYTN----TAISSQPS-PPYSST 1069
Query: 631 QRAPAR*ST 657
P+ ST
Sbjct: 1070 SPHPSHFST 1078
>SB_29237| Best HMM Match : I-set (HMM E-Value=0)
Length = 869
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/74 (24%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Frame = -2
Query: 416 LVINDLSGTKLVS*GENGSGQLTCVQESRWETSNLI-SNTNDVLSVQSLQDTARFSPTQI 240
+++N +++ + +G+ CV ++R N + S+ L VQS+ + A+ + +
Sbjct: 83 VIVNQDGSLSIIATQKEDAGEYICVAKARMNKRNYVESSRAAALEVQSICEAAKIASSPS 142
Query: 239 TLMFSAPGSLRRLR 198
+F A G L +LR
Sbjct: 143 QSLFPA-GILIQLR 155
>SB_38076| Best HMM Match : 7tm_1 (HMM E-Value=1.3e-05)
Length = 577
Score = 28.7 bits (61), Expect = 4.5
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 352 SWPLPFSPQDTSLVPERSFITSSSLWYRTPSPTW 453
+WP+P+SPQ +S S + + TP P W
Sbjct: 543 TWPMPWSPQPSSHFWVTSRLEYLLMILETPKPMW 576
>SB_3148| Best HMM Match : Keratin_B2 (HMM E-Value=0.26)
Length = 147
Score = 28.3 bits (60), Expect = 6.0
Identities = 18/56 (32%), Positives = 25/56 (44%)
Frame = -3
Query: 232 CSQHQVR*DGCGSL*IHQCVHSAQTSVRGHSNKQLLGVIFGRCNLSPFIELYCTFT 65
C QHQ + S I+ CV +A TS R Q V N++P ++ T T
Sbjct: 56 CKQHQHQPVCANSTNINPCVQTAPTSTRVCKQHQHQPVCANSTNINPCVQTAPTST 111
Score = 27.9 bits (59), Expect = 7.9
Identities = 18/56 (32%), Positives = 25/56 (44%)
Frame = -3
Query: 232 CSQHQVR*DGCGSL*IHQCVHSAQTSVRGHSNKQLLGVIFGRCNLSPFIELYCTFT 65
C QHQ + S I+ CV +A TS R Q V N++P ++ T T
Sbjct: 27 CKQHQHQPVCVNSTNINPCVQTAPTSTRVCKQHQHQPVCANSTNINPCVQTAPTST 82
>SB_50551| Best HMM Match : Extensin_2 (HMM E-Value=0.41)
Length = 376
Score = 24.2 bits (50), Expect(2) = 7.0
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = +1
Query: 496 TAYDFWTATYSNPTRSSRWPRCCSTR*RRALAQSSRPA*PPWTTPQR 636
T D + Y R S+ P TR R+ SSR PPW PQ+
Sbjct: 206 TTVDSAQSRYPRGHRKSQIPPW--TR-ERSPVDSSRARSPPWAAPQQ 249
Score = 22.2 bits (45), Expect(2) = 7.0
Identities = 6/10 (60%), Positives = 8/10 (80%)
Frame = +1
Query: 613 PPWTTPQRAP 642
PPWT P++ P
Sbjct: 270 PPWTAPEQDP 279
>SB_7532| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 416
Score = 27.9 bits (59), Expect = 7.9
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +2
Query: 92 RAEVTPPEDDPKQLFVAMTSDRGLCG 169
RA TPPE K + + S GLCG
Sbjct: 263 RARATPPEVTDKMAILRLPSGLGLCG 288
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,675,420
Number of Sequences: 59808
Number of extensions: 450011
Number of successful extensions: 1400
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1275
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1398
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1729817375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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