Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20950
         (691 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1al...   179   3e-47
X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alp...   175   3e-46
EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor 1-a...   173   1e-45
AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor 1-a...   173   1e-45
AF069739-1|AAC63272.2|  690|Apis mellifera translation initiatio...    33   0.003
AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase pro...    23   2.7  
D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.    23   3.6  
AY569781-1|AAS75781.1|  461|Apis mellifera neuronal nicotinic ac...    22   6.3  
AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase pro...    21   8.4  

>AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1alpha
           F2 protein.
          Length = 461

 Score =  179 bits (435), Expect = 3e-47
 Identities = 84/84 (100%), Positives = 84/84 (100%)
 Frame = +3

Query: 3   TIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRD 182
           TIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRD
Sbjct: 38  TIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRD 97

Query: 183 FIKNMITGTSQADCAVLIVAAGTG 254
           FIKNMITGTSQADCAVLIVAAGTG
Sbjct: 98  FIKNMITGTSQADCAVLIVAAGTG 121



 Score =  173 bits (421), Expect = 1e-45
 Identities = 82/85 (96%), Positives = 83/85 (97%)
 Frame = +2

Query: 257 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 436
           FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIG
Sbjct: 123 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIG 182

Query: 437 YNPAAVAFVPISGWHGDNMLEPSTK 511
           YNPAAVAFVPISGWHGDNMLE S+K
Sbjct: 183 YNPAAVAFVPISGWHGDNMLEVSSK 207



 Score =  125 bits (301), Expect = 5e-31
 Identities = 55/62 (88%), Positives = 58/62 (93%)
 Frame = +1

Query: 505 NQMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVG 684
           ++MPWFKGW VERKEGK +GKCLIEALDAILPP RPTDK LRLPLQDVYKIGGIGTVPVG
Sbjct: 206 SKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKIGGIGTVPVG 265

Query: 685 RV 690
           RV
Sbjct: 266 RV 267


>X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alpha
           protein.
          Length = 461

 Score =  175 bits (426), Expect = 3e-46
 Identities = 82/84 (97%), Positives = 83/84 (98%)
 Frame = +3

Query: 3   TIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRD 182
           TIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRD
Sbjct: 38  TIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRD 97

Query: 183 FIKNMITGTSQADCAVLIVAAGTG 254
           FIKNMITGTSQADCAVLIVAAG G
Sbjct: 98  FIKNMITGTSQADCAVLIVAAGIG 121



 Score =  168 bits (409), Expect = 4e-44
 Identities = 79/85 (92%), Positives = 81/85 (95%)
 Frame = +2

Query: 257 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 436
           FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKKIG
Sbjct: 123 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIG 182

Query: 437 YNPAAVAFVPISGWHGDNMLEPSTK 511
           YN A+VAFVPISGWHGDNMLEPS K
Sbjct: 183 YNTASVAFVPISGWHGDNMLEPSPK 207



 Score =  118 bits (284), Expect = 5e-29
 Identities = 52/59 (88%), Positives = 56/59 (94%)
 Frame = +1

Query: 514 PWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 690
           PW+KGW+VERK+G ADGK LIEALDAILPP+RPTDK LRLPLQDVYKIGGIGTVPVGRV
Sbjct: 209 PWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRV 267


>EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor
           1-alpha protein.
          Length = 172

 Score =  173 bits (421), Expect = 1e-45
 Identities = 82/85 (96%), Positives = 83/85 (97%)
 Frame = +2

Query: 257 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 436
           FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIG
Sbjct: 50  FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIG 109

Query: 437 YNPAAVAFVPISGWHGDNMLEPSTK 511
           YNPAAVAFVPISGWHGDNMLE S+K
Sbjct: 110 YNPAAVAFVPISGWHGDNMLEVSSK 134



 Score =  105 bits (251), Expect = 5e-25
 Identities = 48/48 (100%), Positives = 48/48 (100%)
 Frame = +3

Query: 111 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 254
           DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG
Sbjct: 1   DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 48



 Score = 80.6 bits (190), Expect = 1e-17
 Identities = 33/39 (84%), Positives = 36/39 (92%)
 Frame = +1

Query: 505 NQMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDK 621
           ++MPWFKGW VERKEGK +GKCLIEALDAILPP RPTDK
Sbjct: 133 SKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDK 171


>AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor
           1-alpha protein.
          Length = 274

 Score =  173 bits (421), Expect = 1e-45
 Identities = 82/85 (96%), Positives = 83/85 (97%)
 Frame = +2

Query: 257 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 436
           FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIG
Sbjct: 66  FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIG 125

Query: 437 YNPAAVAFVPISGWHGDNMLEPSTK 511
           YNPAAVAFVPISGWHGDNMLE S+K
Sbjct: 126 YNPAAVAFVPISGWHGDNMLEVSSK 150



 Score =  138 bits (333), Expect = 6e-35
 Identities = 64/64 (100%), Positives = 64/64 (100%)
 Frame = +3

Query: 63  WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 242
           WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1   WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60

Query: 243 AGTG 254
           AGTG
Sbjct: 61  AGTG 64



 Score =  125 bits (301), Expect = 5e-31
 Identities = 55/62 (88%), Positives = 58/62 (93%)
 Frame = +1

Query: 505 NQMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVG 684
           ++MPWFKGW VERKEGK +GKCLIEALDAILPP RPTDK LRLPLQDVYKIGGIGTVPVG
Sbjct: 149 SKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKIGGIGTVPVG 208

Query: 685 RV 690
           RV
Sbjct: 209 RV 210


>AF069739-1|AAC63272.2|  690|Apis mellifera translation initiation
           factor 2 protein.
          Length = 690

 Score = 33.1 bits (72), Expect = 0.003
 Identities = 16/35 (45%), Positives = 18/35 (51%)
 Frame = +3

Query: 150 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 254
           VT +D PGH  FI     G    D  VL+VAA  G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229


>AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase
           protein.
          Length = 567

 Score = 23.0 bits (47), Expect = 2.7
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +3

Query: 135 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 236
           T KYY    D P +  FIKN+   ++ +D   LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327


>D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.
          Length = 567

 Score = 22.6 bits (46), Expect = 3.6
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +3

Query: 135 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 236
           T KYY    D P +  FIKN+   ++ +D   L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327


>AY569781-1|AAS75781.1|  461|Apis mellifera neuronal nicotinic
           acetylcholine Apisa7-2 subunit protein.
          Length = 461

 Score = 21.8 bits (44), Expect = 6.3
 Identities = 9/29 (31%), Positives = 14/29 (48%)
 Frame = -1

Query: 526 P*TKAFG*RLQHVVSVPSRNGHESDSSWV 440
           P    FG  L H++ V  +N   + + WV
Sbjct: 30  PLAVVFGLSLHHIIDVDEKNQILTTNCWV 58


>AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase
           protein.
          Length = 580

 Score = 21.4 bits (43), Expect = 8.4
 Identities = 7/20 (35%), Positives = 13/20 (65%)
 Frame = +3

Query: 354 WIPLNHHTVSPDLRKSRRKY 413
           W+P+N +  S +L   +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 218,342
Number of Sequences: 438
Number of extensions: 4667
Number of successful extensions: 19
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21073995
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)