Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20946
(753 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 122 7e-29
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 122 7e-29
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 80 4e-16
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 59 6e-10
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 54 2e-08
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 38 0.001
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 0.002
SPBC1198.03c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 34 0.019
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 33 0.058
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.71
SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 29 0.94
SPCC4B3.07 |||nuclear pore associated protein|Schizosaccharomyce... 28 1.2
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 27 2.9
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 5.0
SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 6.6
SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 26 6.6
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 25 8.8
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 122 bits (293), Expect = 7e-29
Identities = 54/99 (54%), Positives = 73/99 (73%), Gaps = 6/99 (6%)
Frame = +1
Query: 469 GACR--P*QGLCWFRAGLHGWAFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTKKW 642
G C+ P +G F +GLHGWAFT++QF+ YA KF ID K+M RLWGEN+FNP+TKKW
Sbjct: 198 GDCQVFPDKGTVAFASGLHGWAFTVRQFANRYAKKFGIDRNKMMQRLWGENYFNPKTKKW 257
Query: 643 SKQKDD----DNKRSFCMYVLDPIYKVFDAIMKFKKEEI 747
SK D N+R+F M++LDPIY++FDA+M +K+E+
Sbjct: 258 SKSATDANGNSNQRAFNMFILDPIYRIFDAVMNSRKDEV 296
Score = 99 bits (238), Expect = 3e-22
Identities = 51/81 (62%), Positives = 63/81 (77%), Gaps = 1/81 (1%)
Frame = +3
Query: 3 RAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLID 179
+AG+ RF DTR DEQ+R +TIKSTAIS+F E+ + D+ D +E ++ FL+NLID
Sbjct: 50 KAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLID 104
Query: 180 SPGHVDFSSEVTAALRVTDGA 242
SPGHVDFSSEVTAALRVTDGA
Sbjct: 105 SPGHVDFSSEVTAALRVTDGA 125
Score = 86.6 bits (205), Expect = 3e-18
Identities = 41/82 (50%), Positives = 56/82 (68%)
Frame = +2
Query: 260 VSGVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIAT 439
+ GVCVQTETVLRQA+ ERI+P++ +NK+DR YQ F R+VE+VNV+I+T
Sbjct: 132 IEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVIST 191
Query: 440 YNDDGGPMGEVRVDPSKGSVGF 505
Y D +G+ +V P KG+V F
Sbjct: 192 YYDK--VLGDCQVFPDKGTVAF 211
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 122 bits (293), Expect = 7e-29
Identities = 54/99 (54%), Positives = 73/99 (73%), Gaps = 6/99 (6%)
Frame = +1
Query: 469 GACR--P*QGLCWFRAGLHGWAFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTKKW 642
G C+ P +G F +GLHGWAFT++QF+ YA KF ID K+M RLWGEN+FNP+TKKW
Sbjct: 198 GDCQVFPDKGTVAFASGLHGWAFTVRQFANRYAKKFGIDRNKMMQRLWGENYFNPKTKKW 257
Query: 643 SKQKDD----DNKRSFCMYVLDPIYKVFDAIMKFKKEEI 747
SK D N+R+F M++LDPIY++FDA+M +K+E+
Sbjct: 258 SKSATDANGNSNQRAFNMFILDPIYRIFDAVMNSRKDEV 296
Score = 99 bits (238), Expect = 3e-22
Identities = 51/81 (62%), Positives = 63/81 (77%), Gaps = 1/81 (1%)
Frame = +3
Query: 3 RAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLID 179
+AG+ RF DTR DEQ+R +TIKSTAIS+F E+ + D+ D +E ++ FL+NLID
Sbjct: 50 KAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLID 104
Query: 180 SPGHVDFSSEVTAALRVTDGA 242
SPGHVDFSSEVTAALRVTDGA
Sbjct: 105 SPGHVDFSSEVTAALRVTDGA 125
Score = 86.6 bits (205), Expect = 3e-18
Identities = 41/82 (50%), Positives = 56/82 (68%)
Frame = +2
Query: 260 VSGVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIAT 439
+ GVCVQTETVLRQA+ ERI+P++ +NK+DR YQ F R+VE+VNV+I+T
Sbjct: 132 IEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVIST 191
Query: 440 YNDDGGPMGEVRVDPSKGSVGF 505
Y D +G+ +V P KG+V F
Sbjct: 192 YYDK--VLGDCQVFPDKGTVAF 211
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 79.8 bits (188), Expect = 4e-16
Identities = 44/79 (55%), Positives = 58/79 (73%)
Frame = +3
Query: 6 AGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSP 185
AG RF D R+DE R IT+KS+AIS+FF++ I+ D++ + EK +LINLIDSP
Sbjct: 51 AGTVRFLDFREDEITRGITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSP 102
Query: 186 GHVDFSSEVTAALRVTDGA 242
GHVDFSSEV++A R+ DGA
Sbjct: 103 GHVDFSSEVSSASRLCDGA 121
Score = 54.8 bits (126), Expect = 1e-08
Identities = 29/61 (47%), Positives = 35/61 (57%)
Frame = +2
Query: 260 VSGVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIAT 439
V GVC QT TVLRQA +RIK IL +NKMDR + R+VE VN +I T
Sbjct: 128 VEGVCSQTITVLRQAWIDRIKVILVINKMDRLITELKLSPIEAHYHLLRLVEQVNAVIGT 187
Query: 440 Y 442
+
Sbjct: 188 F 188
Score = 54.4 bits (125), Expect = 2e-08
Identities = 29/91 (31%), Positives = 47/91 (51%), Gaps = 3/91 (3%)
Frame = +1
Query: 481 P*QGLCWFRAGLHGWAFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTKKWSKQKDD 660
P QG F + GWAF L QFSE Y K + L LWG+ + +P+TK+ + K
Sbjct: 212 PEQGNVVFASAYDGWAFCLDQFSEFYEKKLGLKQKALTKCLWGDYYLDPKTKRVLQPKHL 271
Query: 661 DNKR---SFCMYVLDPIYKVFDAIMKFKKEE 744
+R F +VL+ ++ V+++ + + E
Sbjct: 272 QGRRLKPMFVQFVLENLWAVYESAVSNRNLE 302
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 59.3 bits (137), Expect = 6e-10
Identities = 27/78 (34%), Positives = 49/78 (62%), Gaps = 2/78 (2%)
Frame = +1
Query: 520 GWAFTLKQFSEMYADKFK-IDLVKLMNRLWGENFFNPQTKKWSKQK-DDDNKRSFCMYVL 693
G+ FTL F+++Y D+ ID+ RLWG+ +F+ +T+K++KQ D RSF ++L
Sbjct: 326 GYCFTLSSFAKLYIDRHGGIDVDLFSKRLWGDIYFDSKTRKFAKQSLDGSGVRSFVHFIL 385
Query: 694 DPIYKVFDAIMKFKKEEI 747
+P+YK+ + + E++
Sbjct: 386 EPLYKLHTLTISDEAEKL 403
Score = 41.9 bits (94), Expect = 9e-05
Identities = 26/74 (35%), Positives = 40/74 (54%)
Frame = +3
Query: 18 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVD 197
R+TDT E++R ++IKST +++ D + K+ F ID+PGHVD
Sbjct: 177 RYTDTHYLERERVMSIKSTPLTLAVS------------DMKGKT---FAFQCIDTPGHVD 221
Query: 198 FSSEVTAALRVTDG 239
F EV A + ++DG
Sbjct: 222 FVDEVAAPMAISDG 235
Score = 37.1 bits (82), Expect = 0.003
Identities = 22/88 (25%), Positives = 39/88 (44%)
Frame = +2
Query: 260 VSGVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIAT 439
+ GV + T +++ AI + +L +NK+DR Y + +++ VN I
Sbjct: 243 IEGVMINTTRIIKHAILHDMPIVLVLNKVDRLILELRLPPNDAYHKLRHVIDEVNDNICQ 302
Query: 440 YNDDGGPMGEVRVDPSKGSVGFGLVFMG 523
+ D + RV P G+V F +G
Sbjct: 303 ISKD----LKYRVSPELGNVCFASCDLG 326
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 54.4 bits (125), Expect = 2e-08
Identities = 29/67 (43%), Positives = 41/67 (61%)
Frame = +3
Query: 42 EQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAA 221
E+++ ITI+S A +E + N Q+ EK + IN+ID+PGH+DF+ EV A
Sbjct: 108 EREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERA 165
Query: 222 LRVTDGA 242
LRV DGA
Sbjct: 166 LRVLDGA 172
Score = 29.9 bits (64), Expect = 0.41
Identities = 17/31 (54%), Positives = 19/31 (61%)
Frame = +2
Query: 260 VSGVCVQTETVLRQAIAERIKPILFMNKMDR 352
VSGV QT TV RQ + I F+NKMDR
Sbjct: 179 VSGVQSQTITVDRQMRRYNVPRISFVNKMDR 209
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 38.3 bits (85), Expect = 0.001
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = +3
Query: 153 KGFLINLIDSPGHVDFSSEVTAALRVTDG 239
+ +L+NLID+PGHVDF +EV +L +G
Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEG 150
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 37.9 bits (84), Expect = 0.002
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +3
Query: 165 INLIDSPGHVDFSSEVTAALRVTDGA 242
INLID+PGH DF+ EV ++ V DGA
Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGA 120
Score = 31.5 bits (68), Expect = 0.13
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = +2
Query: 263 SGVCVQTETVLRQAIAERIKPILFMNKMDR 352
+GV QT+ V +QA I ++F+NKMDR
Sbjct: 128 AGVEAQTKVVWKQATKRGIPKVIFVNKMDR 157
>SPBC1198.03c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 249
Score = 34.3 bits (75), Expect = 0.019
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +1
Query: 526 AFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQ 630
A+ + + E Y DK K ++K +W FFNP+
Sbjct: 153 AYVISYYVEKYVDKAKFPIIKGFIEMWNRKFFNPR 187
>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
Apc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1458
Score = 32.7 bits (71), Expect = 0.058
Identities = 15/45 (33%), Positives = 29/45 (64%), Gaps = 1/45 (2%)
Frame = +1
Query: 529 FTLKQFS-EMYADKFKIDLVKLMNRLWGENFFNPQTKKWSKQKDD 660
FT+ + + E Y+ ++ + ++L+NRL ++F +P+T KW K D
Sbjct: 620 FTVLEIAAEEYSPNWESEELRLVNRLDVDSFLHPKTPKWVFNKQD 664
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.1 bits (62), Expect = 0.71
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -1
Query: 528 SPPMKTSPKPTEPLLGSTRTSPMGPPSS 445
SPP +P P + ST P+ PPSS
Sbjct: 270 SPPRPIAPVSMNPAINSTSKPPLPPPSS 297
>SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 345
Score = 28.7 bits (61), Expect = 0.94
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -2
Query: 269 HQTHSNNHKGSISDTECSCYFRREINVSR 183
+Q H +HKG+I D+ Y E +SR
Sbjct: 272 YQRHKKSHKGAIQDSSIQSYLDEEEKLSR 300
>SPCC4B3.07 |||nuclear pore associated protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 393
Score = 28.3 bits (60), Expect = 1.2
Identities = 10/38 (26%), Positives = 22/38 (57%)
Frame = +1
Query: 535 LKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTKKWSK 648
L QF + +++ F +D+ ++ LW ++ +P T + K
Sbjct: 242 LFQFGDQFSEAFLLDVCSIITALWLKSVVDPNTPAYYK 279
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 27.1 bits (57), Expect = 2.9
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -3
Query: 205 EEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 29
EE + P + K KK S F+ T +S S++NI+ A+DL+ + S S ++
Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -1
Query: 546 ELFEGESPPMKTSPKPTEPLLGSTRTSPMGP 454
EL SP P P +PLL S SP+ P
Sbjct: 1045 ELSRNNSPRPDFLPLPNQPLLHSNLHSPVSP 1075
>SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting
endonuclease Cce1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 258
Score = 25.8 bits (54), Expect = 6.6
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -3
Query: 157 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLMVMQR 50
P S +S W+ V+NTK SFS ++M +L+ Q+
Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203
>SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 986
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/27 (33%), Positives = 14/27 (51%)
Frame = +2
Query: 245 CGCYCVSGVCVQTETVLRQAIAERIKP 325
CG +C+ G T T LR+ ++ P
Sbjct: 253 CGYFCIMGEIAMTLTKLRELFPSKLTP 279
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.4 bits (53), Expect = 8.8
Identities = 10/35 (28%), Positives = 18/35 (51%)
Frame = +1
Query: 487 QGLCWFRAGLHGWAFTLKQFSEMYADKFKIDLVKL 591
QG + L W + K ++E YA+ + L+K+
Sbjct: 2713 QGYVYTPRDLTRWLISFKNYAESYAETNNLSLIKV 2747
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,076,697
Number of Sequences: 5004
Number of extensions: 62779
Number of successful extensions: 224
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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