Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20946
(753 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 98 6e-21
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 64 7e-11
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 62 3e-10
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 62 3e-10
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 53 2e-07
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 51 9e-07
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 45 5e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 45 5e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 45 6e-05
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 41 0.001
At5g13650.2 68418.m01585 elongation factor family protein contai... 36 0.038
At5g13650.1 68418.m01584 elongation factor family protein contai... 36 0.038
At2g44790.1 68415.m05574 uclacyanin II strong similarity to ucla... 33 0.15
At2g31060.1 68415.m03790 elongation factor family protein contai... 30 1.4
At1g10470.1 68414.m01179 two-component responsive regulator / re... 30 1.9
At5g59400.2 68418.m07444 expressed protein predicted protein, Ar... 28 5.8
At5g28760.1 68418.m03532 hypothetical protein contains Pfam prof... 28 7.7
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 28 7.7
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 97.9 bits (233), Expect = 6e-21
Identities = 46/83 (55%), Positives = 58/83 (69%)
Frame = +2
Query: 257 CVSGVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIA 436
C+ GVCVQTETVLRQA+ ERI+P+L +NKMDR YQTF R++EN NVI+A
Sbjct: 131 CIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSRVIENANVIMA 190
Query: 437 TYNDDGGPMGEVRVDPSKGSVGF 505
TY D +G+V+V P KG+V F
Sbjct: 191 TYEDP--LLGDVQVYPEKGTVAF 211
Score = 95.5 bits (227), Expect = 3e-20
Identities = 49/79 (62%), Positives = 61/79 (77%)
Frame = +3
Query: 6 AGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSP 185
AG+ R TDTR DE +R ITIKST IS+++E+ ++ L T R+ +E +LINLIDSP
Sbjct: 51 AGDVRMTDTRADEAERGITIKSTGISLYYEMTDESLKSFTGA--RDGNE--YLINLIDSP 106
Query: 186 GHVDFSSEVTAALRVTDGA 242
GHVDFSSEVTAALR+TDGA
Sbjct: 107 GHVDFSSEVTAALRITDGA 125
Score = 89.8 bits (213), Expect = 2e-18
Identities = 41/91 (45%), Positives = 57/91 (62%), Gaps = 2/91 (2%)
Frame = +1
Query: 481 P*QGLCWFRAGLHGWAFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTKKWSKQKDD 660
P +G F AGLHGWAFTL F++MYA KF + K+M RLWGENFF+P T+KWS +
Sbjct: 204 PEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVVESKMMERLWGENFFDPATRKWSGKNTG 263
Query: 661 DN--KRSFCMYVLDPIYKVFDAIMKFKKEEI 747
KR F + +PI ++ M +K+++
Sbjct: 264 SPTCKRGFVQFCYEPIKQIIATCMNDQKDKL 294
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 64.5 bits (150), Expect = 7e-11
Identities = 28/93 (30%), Positives = 55/93 (59%), Gaps = 3/93 (3%)
Frame = +1
Query: 481 P*QGLCWFRAGLHGWAFTLKQFSEMYADKFKI--DLVKLMNRLWGENFFNPQTKKWSKQK 654
P G F +G GW+FTL+ F+ MYA + D+ K +RLWG+ +++P T+ ++
Sbjct: 301 PAAGNVCFASGTAGWSFTLQSFARMYAKLHGVAMDVDKFASRLWGDVYYHPDTRVFNTSP 360
Query: 655 D-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEID 750
+R+F ++L+P+YK++ ++ K+ ++
Sbjct: 361 PVGGGERAFVQFILEPLYKIYSQVIGEHKKSVE 393
Score = 59.3 bits (137), Expect = 3e-09
Identities = 34/75 (45%), Positives = 49/75 (65%)
Frame = +3
Query: 18 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVD 197
R+TDTR DEQ+R I+IK+ +S+ E D R KS +L N++D+PG+V+
Sbjct: 163 RYTDTRVDEQERNISIKAVPMSLVLE------------DSRSKS---YLCNIMDTPGNVN 207
Query: 198 FSSEVTAALRVTDGA 242
FS E+TA+LR+ DGA
Sbjct: 208 FSDEMTASLRLADGA 222
Score = 36.3 bits (80), Expect = 0.022
Identities = 21/80 (26%), Positives = 38/80 (47%)
Frame = +2
Query: 266 GVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYN 445
GV V TE +R AI + + ++ +NK+DR Y + +E +N I+ +
Sbjct: 231 GVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRYTIEVINNHISAAS 290
Query: 446 DDGGPMGEVRVDPSKGSVGF 505
+ + +DP+ G+V F
Sbjct: 291 TNAADL--PLIDPAAGNVCF 308
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 62.5 bits (145), Expect = 3e-10
Identities = 27/93 (29%), Positives = 55/93 (59%), Gaps = 3/93 (3%)
Frame = +1
Query: 481 P*QGLCWFRAGLHGWAFTLKQFSEMYADKFKI--DLVKLMNRLWGENFFNPQTKKWSKQK 654
P G F +G GW+FTL+ F++MYA + D+ K +RLWG+ +++ T+ + +
Sbjct: 315 PAAGNVCFASGTAGWSFTLQSFAKMYAKLHGVAMDVDKFASRLWGDVYYHSDTRVFKRSP 374
Query: 655 D-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEID 750
+R+F ++L+P+YK++ ++ K+ ++
Sbjct: 375 PVGGGERAFVQFILEPLYKIYSQVIGEHKKSVE 407
Score = 60.9 bits (141), Expect = 9e-10
Identities = 34/75 (45%), Positives = 49/75 (65%)
Frame = +3
Query: 18 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVD 197
++TDTR DEQ+R I+IK+ +S+ E D R KS +L N++D+PGHV+
Sbjct: 177 KYTDTRVDEQERNISIKAVPMSLVLE------------DSRSKS---YLCNIMDTPGHVN 221
Query: 198 FSSEVTAALRVTDGA 242
FS E+TA+LR+ DGA
Sbjct: 222 FSDEMTASLRLADGA 236
Score = 38.7 bits (86), Expect = 0.004
Identities = 22/80 (27%), Positives = 38/80 (47%)
Frame = +2
Query: 266 GVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYN 445
GV V TE +R AI + + ++ +NK+DR Y + +E +N I+ +
Sbjct: 245 GVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAAS 304
Query: 446 DDGGPMGEVRVDPSKGSVGF 505
G + +DP+ G+V F
Sbjct: 305 TTAGDL--PLIDPAAGNVCF 322
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 62.5 bits (145), Expect = 3e-10
Identities = 27/93 (29%), Positives = 55/93 (59%), Gaps = 3/93 (3%)
Frame = +1
Query: 481 P*QGLCWFRAGLHGWAFTLKQFSEMYADKFKI--DLVKLMNRLWGENFFNPQTKKWSKQK 654
P G F +G GW+FTL+ F++MYA + D+ K +RLWG+ +++ T+ + +
Sbjct: 315 PAAGNVCFASGTAGWSFTLQSFAKMYAKLHGVAMDVDKFASRLWGDVYYHSDTRVFKRSP 374
Query: 655 D-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEID 750
+R+F ++L+P+YK++ ++ K+ ++
Sbjct: 375 PVGGGERAFVQFILEPLYKIYSQVIGEHKKSVE 407
Score = 60.9 bits (141), Expect = 9e-10
Identities = 34/75 (45%), Positives = 49/75 (65%)
Frame = +3
Query: 18 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVD 197
++TDTR DEQ+R I+IK+ +S+ E D R KS +L N++D+PGHV+
Sbjct: 177 KYTDTRVDEQERNISIKAVPMSLVLE------------DSRSKS---YLCNIMDTPGHVN 221
Query: 198 FSSEVTAALRVTDGA 242
FS E+TA+LR+ DGA
Sbjct: 222 FSDEMTASLRLADGA 236
Score = 38.7 bits (86), Expect = 0.004
Identities = 22/80 (27%), Positives = 38/80 (47%)
Frame = +2
Query: 266 GVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYN 445
GV V TE +R AI + + ++ +NK+DR Y + +E +N I+ +
Sbjct: 245 GVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAAS 304
Query: 446 DDGGPMGEVRVDPSKGSVGF 505
G + +DP+ G+V F
Sbjct: 305 TTAGDL--PLIDPAAGNVCF 322
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 53.2 bits (122), Expect = 2e-07
Identities = 33/79 (41%), Positives = 46/79 (58%)
Frame = +3
Query: 6 AGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSP 185
AG+ RF D +EQ R IT+KS++IS+ + K + +NLIDSP
Sbjct: 43 AGKLRFMDYLDEEQRRAITMKSSSISLKY--------------------KDYSLNLIDSP 82
Query: 186 GHVDFSSEVTAALRVTDGA 242
GH+DF SEV+ A R++DGA
Sbjct: 83 GHMDFCSEVSTAARLSDGA 101
Score = 52.8 bits (121), Expect = 2e-07
Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 4/87 (4%)
Frame = +1
Query: 478 RP*QGLCWFRAGLHGWAFTLKQFSEMYADKFKIDLVKLMNRLWGENFFNPQTK----KWS 645
+P +G F L GW F + +F+ YA K L LWG ++ P+TK K +
Sbjct: 205 QPQKGNVVFVCALDGWGFGIAEFANFYASKLGASATALQKSLWGPRYYIPKTKMIVGKKN 264
Query: 646 KQKDDDNKRSFCMYVLDPIYKVFDAIM 726
K F +VL+P+++V++A +
Sbjct: 265 LSAGSKAKPMFVQFVLEPLWQVYEAAL 291
Score = 48.8 bits (111), Expect = 4e-06
Identities = 25/64 (39%), Positives = 33/64 (51%)
Frame = +2
Query: 260 VSGVCVQTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIAT 439
V GV +QT VLRQA E++ P L +NK+DR Y RIV VN I++
Sbjct: 108 VEGVHIQTHAVLRQAWIEKLTPCLVLNKIDRLIFELRLSPMEAYTRLIRIVHEVNGIVSA 167
Query: 440 YNDD 451
Y +
Sbjct: 168 YKSE 171
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 50.8 bits (116), Expect = 9e-07
Identities = 29/78 (37%), Positives = 47/78 (60%)
Frame = +3
Query: 9 GETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPG 188
G+ ++ D K +++R IT+K+ +MF+E + +D + G+L+NLID+PG
Sbjct: 98 GQPQYLD--KLQRERGITVKAQTATMFYENKVED-----------QEASGYLLNLIDTPG 144
Query: 189 HVDFSSEVTAALRVTDGA 242
HVDFS EV+ +L GA
Sbjct: 145 HVDFSYEVSRSLSACQGA 162
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 45.2 bits (102), Expect = 5e-05
Identities = 19/30 (63%), Positives = 24/30 (80%)
Frame = +3
Query: 153 KGFLINLIDSPGHVDFSSEVTAALRVTDGA 242
K + +N+ID+PGHVDF+ EV ALRV DGA
Sbjct: 131 KDYKVNIIDTPGHVDFTIEVERALRVLDGA 160
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 45.2 bits (102), Expect = 5e-05
Identities = 19/30 (63%), Positives = 24/30 (80%)
Frame = +3
Query: 153 KGFLINLIDSPGHVDFSSEVTAALRVTDGA 242
K + +N+ID+PGHVDF+ EV ALRV DGA
Sbjct: 131 KDYKVNIIDTPGHVDFTIEVERALRVLDGA 160
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 44.8 bits (101), Expect = 6e-05
Identities = 26/59 (44%), Positives = 36/59 (61%)
Frame = +3
Query: 66 KSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 242
+ TA + E E++ + IT+ +K IN+ID+PGHVDF+ EV ALRV DGA
Sbjct: 131 EGTATMDWMEQEQERGITITSAATTTFWDK-HRINIIDTPGHVDFTLEVERALRVLDGA 188
Score = 32.3 bits (70), Expect = 0.36
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +2
Query: 260 VSGVCVQTETVLRQAIAERIKPILFMNKMDR 352
V+GV Q+ETV RQA + I F+NKMDR
Sbjct: 195 VAGVEPQSETVWRQADKYGVPRICFVNKMDR 225
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 40.7 bits (91), Expect = 0.001
Identities = 17/28 (60%), Positives = 22/28 (78%)
Frame = +3
Query: 159 FLINLIDSPGHVDFSSEVTAALRVTDGA 242
F +NLID+PGHVDFS EV+ +L +GA
Sbjct: 152 FCLNLIDTPGHVDFSYEVSRSLAACEGA 179
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 35.5 bits (78), Expect = 0.038
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +3
Query: 153 KGFLINLIDSPGHVDFSSEVTAALRVTDG 239
K +N+ID+PGH DF EV L + DG
Sbjct: 144 KNTKVNIIDTPGHSDFGGEVERVLNMVDG 172
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 35.5 bits (78), Expect = 0.038
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +3
Query: 153 KGFLINLIDSPGHVDFSSEVTAALRVTDG 239
K +N+ID+PGH DF EV L + DG
Sbjct: 143 KNTKVNIIDTPGHSDFGGEVERVLNMVDG 171
>At2g44790.1 68415.m05574 uclacyanin II strong similarity to
uclacyanin II GI:3399769 from [Arabidopsis thaliana];
contains Pfam profile PF02298: Plastocyanin-like domain;
identical to cDNA uclacyanin II GI:3399768
Length = 202
Score = 33.5 bits (73), Expect = 0.15
Identities = 15/29 (51%), Positives = 17/29 (58%)
Frame = -1
Query: 531 ESPPMKTSPKPTEPLLGSTRTSPMGPPSS 445
ESPP SP PT P G+ TSP PP +
Sbjct: 149 ESPPSGGSPTPTTPTPGAGSTSPPPPPKA 177
>At2g31060.1 68415.m03790 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain, PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 527
Score = 30.3 bits (65), Expect = 1.4
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +2
Query: 266 GVCVQTETVLRQAIAERIKPILFMNKMDR 352
G QT+ VL +A+ ++PIL +NK+DR
Sbjct: 14 GPLAQTKFVLAKALKYGLRPILLLNKVDR 42
>At1g10470.1 68414.m01179 two-component responsive regulator /
response regulator 4 (ARR4) identical to responce
regulator1 GI:3273195 from [Arabidopsis thaliana];
identical to cDNA ARR4 mRNA for response regulator 4
GI:3953596
Length = 259
Score = 29.9 bits (64), Expect = 1.9
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = -1
Query: 528 SPPMKTSPKPTEPLLGSTRTSPMGPPSS 445
SPP+ SP+ + PL ST +S PP S
Sbjct: 190 SPPLTISPESSPPLTVSTESSDSSPPLS 217
>At5g59400.2 68418.m07444 expressed protein predicted protein,
Arabidopsis thaliana
Length = 301
Score = 28.3 bits (60), Expect = 5.8
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = -2
Query: 290 LFRFVHTHQTHSNNHKGSISDTECSCYFRREINVSR 183
+F FV + Q++ + HK EC+ FR ++ V +
Sbjct: 237 VFAFVRSDQSNRSAHKADCHVCECTLEFRTKVEVDK 272
>At5g28760.1 68418.m03532 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 520
Score = 27.9 bits (59), Expect = 7.7
Identities = 18/71 (25%), Positives = 40/71 (56%), Gaps = 2/71 (2%)
Frame = +1
Query: 544 FSEMYADKFKIDLVKLMNRLWGENFFNPQTKKWSKQKDDDNKRSFCM-YVLDPIYKVFDA 720
F ++ D ++ L+K ++W ++ F+ + S Q+ ++ S + +VLD I + +A
Sbjct: 337 FIDLVVDSWRTRLIKEKKKIWWKDMFDHDVRSRSDQQMEEQHISENVPHVLD-ISSLKEA 395
Query: 721 I-MKFKKEEID 750
I FK+E+++
Sbjct: 396 IDADFKREKVN 406
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 27.9 bits (59), Expect = 7.7
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2
Query: 482 GRHAPHPWDHHHR 444
G HAPHP+D+H R
Sbjct: 375 GPHAPHPYDYHPR 387
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,264,936
Number of Sequences: 28952
Number of extensions: 338007
Number of successful extensions: 1241
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1226
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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