BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20934 (658 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g01190.1 68417.m00157 phosphatidylinositol-4-phosphate 5-kina... 29 3.6 At4g25830.1 68417.m03715 integral membrane family protein contai... 28 6.3 >At4g01190.1 68417.m00157 phosphatidylinositol-4-phosphate 5-kinase family protein similar to phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1 [Arabidopsis thaliana] GI:3702691; contains Pfam profile PF01504: Phosphatidylinositol-4-phosphate 5-Kinase Length = 401 Score = 28.7 bits (61), Expect = 3.6 Identities = 24/80 (30%), Positives = 43/80 (53%), Gaps = 4/80 (5%) Frame = +2 Query: 350 VNDAEDMRVS---RNHAEIEVTDTCVILKSLHQNPCFYIDKLSNSKRTLTQNNAQLYVM- 517 VN D++ S R++ +IEV +T V LK + + CFY+D L+ +R + Q ++ Sbjct: 147 VNKLYDLKGSPKGRSNKKIEVRNTTV-LKDIDFDFCFYVDPLAR-QRIIKQTKLDCELLE 204 Query: 518 VINLVYYLIHIGLN*YGAHQ 577 ++ Y + +GL G+ Q Sbjct: 205 EEGIMDYSLLVGLQSKGSCQ 224 >At4g25830.1 68417.m03715 integral membrane family protein contains TIGRFAM TIGR01569 : plant integral membrane protein TIGR01569; contains Pfam PF04535 : Domain of unknown function (DUF588) Length = 175 Score = 27.9 bits (59), Expect = 6.3 Identities = 18/56 (32%), Positives = 26/56 (46%) Frame = +2 Query: 410 TCVILKSLHQNPCFYIDKLSNSKRTLTQNNAQLYVMVINLVYYLIHIGLN*YGAHQ 577 +C+I YI K + S R L A+LY+ V+ Y L+ +GL Y Q Sbjct: 23 SCLIGLDSQTKEIAYIHK-NVSFRYLLALEAELYIDVVVAAYNLVQLGLGWYNVEQ 77 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,433,274 Number of Sequences: 28952 Number of extensions: 273528 Number of successful extensions: 596 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 588 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 596 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1373722560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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