Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20933
(751 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20890.1 68418.m02481 chaperonin, putative similar to SWISS-P... 147 6e-36
At3g11830.1 68416.m01450 chaperonin, putative similar to SWISS-P... 41 0.001
At3g20050.1 68416.m02536 T-complex protein 1 alpha subunit / TCP... 39 0.003
At3g02530.1 68416.m00241 chaperonin, putative similar to SWISS-P... 38 0.009
At5g16070.1 68418.m01878 chaperonin, putative similar to SWISS-P... 37 0.012
At3g18190.1 68416.m02314 chaperonin, putative similar to SWISS-P... 32 0.35
At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 30 1.4
At1g71010.1 68414.m08192 phosphatidylinositol-4-phosphate 5-kina... 30 1.9
At5g48300.1 68418.m05966 glucose-1-phosphate adenylyltransferase... 29 3.3
At1g24510.2 68414.m03085 T-complex protein 1 epsilon subunit, pu... 29 3.3
At1g24510.1 68414.m03086 T-complex protein 1 epsilon subunit, pu... 29 3.3
At1g19700.1 68414.m02457 homeobox-leucine zipper family protein ... 29 3.3
At4g33240.1 68417.m04731 phosphatidylinositol-4-phosphate 5-kina... 29 4.4
At3g63150.1 68416.m07092 GTP-binding protein-related low similar... 28 5.8
At1g05800.1 68414.m00606 lipase class 3 family protein similar t... 28 5.8
>At5g20890.1 68418.m02481 chaperonin, putative similar to
SWISS-PROT:P78371- T-complex protein 1, beta subunit
(TCP-1-beta) [Homo sapiens]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 527
Score = 147 bits (357), Expect = 6e-36
Identities = 63/83 (75%), Positives = 75/83 (90%)
Frame = +1
Query: 502 CLLNRQLIYNYPEQLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKL 681
C +NRQLIYN+PE+LFADAG++AIEHADFEGIERLGLVTGGEI STFD+P+ VKLGHCKL
Sbjct: 284 CFVNRQLIYNFPEELFADAGILAIEHADFEGIERLGLVTGGEIASTFDNPESVKLGHCKL 343
Query: 682 IEEVLIGDESLIRFSGVALGSAC 750
IEE++IG++ LI FSG +G AC
Sbjct: 344 IEEIMIGEDKLIHFSGCEMGQAC 366
Score = 130 bits (314), Expect = 1e-30
Identities = 56/89 (62%), Positives = 75/89 (84%)
Frame = +2
Query: 257 GLLEESFLDEGFLLNKKVGVHQPKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAE 436
G L++SFLDEGF+L+KK+G+ QPK++ENANIL+ANT MDTDK+K++G+ ++VDSM K+AE
Sbjct: 202 GSLKDSFLDEGFILDKKIGIGQPKRIENANILVANTAMDTDKVKIYGARVRVDSMTKVAE 261
Query: 437 LEVAEKEKMKDKVNKILAHKCNVFLTDNL 523
+E AEKEKMKDKV KI+ H N F+ L
Sbjct: 262 IEGAEKEKMKDKVKKIIGHGINCFVNRQL 290
Score = 89.4 bits (212), Expect = 2e-18
Identities = 53/102 (51%), Positives = 67/102 (65%)
Frame = +3
Query: 6 TIIAGWRIASDAAKQALAEASFDHQKNLNEASLRVDLENIARTTLSSKILSNHKEHFTKL 185
TIIAG+R+AS+ A+ AL + D++ N + R DL IA TTL SKILS KEHF ++
Sbjct: 120 TIIAGYRMASECARNALLKRVIDNKDNAEK--FRSDLLKIAMTTLCSKILSQDKEHFAEM 177
Query: 186 AVDAVLRLKGSGNLKAIQIIKISEVFLKNHSWMKGSC*TKKL 311
AVDAV RLKGS NL+AIQIIK LK+ +G KK+
Sbjct: 178 AVDAVFRLKGSTNLEAIQIIKKPGGSLKDSFLDEGFILDKKI 219
>At3g11830.1 68416.m01450 chaperonin, putative similar to
SWISS-PROT:P80313 T-complex protein 1, eta subunit
(TCP-1-eta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 557
Score = 40.7 bits (91), Expect = 0.001
Identities = 27/83 (32%), Positives = 39/83 (46%)
Frame = +3
Query: 3 QTIIAGWRIASDAAKQALAEASFDHQKNLNEASLRVDLENIARTTLSSKILSNHKEHFTK 182
Q +I +R AS A + E + + E + L A TTLSSK++ KE F
Sbjct: 122 QNLIRSYRTASTLAIAKVKELAVSIEGKSVEEKKGL-LAKCAATTLSSKLIGGEKEFFAT 180
Query: 183 LAVDAVLRLKGSGNLKAIQIIKI 251
+ VDAV+ + L I I K+
Sbjct: 181 MVVDAVMAIGNDDRLNLIGIKKV 203
Score = 38.3 bits (85), Expect = 0.005
Identities = 22/79 (27%), Positives = 36/79 (45%)
Frame = +1
Query: 505 LLNRQLIYNYPEQLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLI 684
+L+R I + Q FAD + E + R+ GG + ++ ++ LG C++
Sbjct: 292 VLSRLAIGDLATQYFADRDIFCAGRVAEEDLNRVAAAAGGTVQTSVNNIIDEVLGTCEIF 351
Query: 685 EEVLIGDESLIRFSGVALG 741
EE +G E FSG G
Sbjct: 352 EEKQVGGERFNIFSGCPSG 370
>At3g20050.1 68416.m02536 T-complex protein 1 alpha subunit /
TCP-1-alpha / chaperonin (CCT1) identical to
SWISS-PROT:P28769- T-complex protein 1, alpha subunit
(TCP-1-alpha) [Arabidopsis thaliana]
Length = 545
Score = 39.1 bits (87), Expect = 0.003
Identities = 23/69 (33%), Positives = 40/69 (57%)
Frame = +3
Query: 6 TIIAGWRIASDAAKQALAEASFDHQKNLNEASLRVDLENIARTTLSSKILSNHKEHFTKL 185
+II+G+R+A + + + E + L +V L N A+T++SSK++S + F L
Sbjct: 119 SIISGYRLAMRESCKYIEEKLVTKVEKLG----KVPLINCAKTSMSSKLISGDSDFFANL 174
Query: 186 AVDAVLRLK 212
V+AVL +K
Sbjct: 175 VVEAVLSVK 183
Score = 31.9 bits (69), Expect = 0.47
Identities = 20/73 (27%), Positives = 32/73 (43%), Gaps = 6/73 (8%)
Frame = +1
Query: 547 FADAGVMAIEHADFEGIERLGLVTGGEIVSTF------DSPDKVKLGHCKLIEEVLIGDE 708
F +AG +A+ E + + TG +V+TF ++ D LG + E I D+
Sbjct: 303 FVEAGAIAVRRVRKEDMRHVAKATGATLVTTFADMEGEETFDPAHLGSADEVVEERIADD 362
Query: 709 SLIRFSGVALGSA 747
+I G SA
Sbjct: 363 DVILIKGTKTSSA 375
>At3g02530.1 68416.m00241 chaperonin, putative similar to
SWISS-PROT:P80317- T-complex protein 1, zeta subunit
(TCP-1-zeta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 535
Score = 37.5 bits (83), Expect = 0.009
Identities = 23/68 (33%), Positives = 36/68 (52%)
Frame = +1
Query: 505 LLNRQLIYNYPEQLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLI 684
+LN++ I L A G++A+ A +ERL L GGE V++ D LG L+
Sbjct: 294 ILNQKGIDPPSLDLLAREGIIALRRAKRRNMERLVLACGGEAVNSVDDLTPDCLGWAGLV 353
Query: 685 EEVLIGDE 708
E ++G+E
Sbjct: 354 YEHVLGEE 361
>At5g16070.1 68418.m01878 chaperonin, putative similar to
SWISS-PROT:P80317 T-complex protein 1, zeta subunit
(TCP-1-zeta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 535
Score = 37.1 bits (82), Expect = 0.012
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = +1
Query: 544 LFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIRF 723
L A G++ + A +ERL L GGE V++ D LG L+ E ++G+E
Sbjct: 306 LLAREGIIGLRRAKRRNMERLVLACGGEAVNSVDDLTPESLGWAGLVYEHVLGEEKYTFV 365
Query: 724 SGVALGSAC 750
V ++C
Sbjct: 366 EQVKNPNSC 374
>At3g18190.1 68416.m02314 chaperonin, putative similar to
SWISS-PROT:P50991- T-complex protein 1, delta subunit
(TCP-1-delta) [Homo sapiens]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 536
Score = 32.3 bits (70), Expect = 0.35
Identities = 16/61 (26%), Positives = 31/61 (50%)
Frame = +1
Query: 550 ADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIRFSG 729
A A +M I+ + + IE + ++ + KLGH L+EE +GD +++ +G
Sbjct: 316 AKAKIMVIKDVERDEIEFVTKTLNCLPIANIEHFRAEKLGHADLVEEASLGDGKILKITG 375
Query: 730 V 732
+
Sbjct: 376 I 376
Score = 28.7 bits (61), Expect = 4.4
Identities = 16/37 (43%), Positives = 22/37 (59%)
Frame = +3
Query: 93 EASLRVDLENIARTTLSSKILSNHKEHFTKLAVDAVL 203
E + R L A T+L+SK++S + LAVDAVL
Sbjct: 152 ELTDRDSLVKSASTSLNSKVVSQYSTLLAPLAVDAVL 188
>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
PF05064 Nsp1-like C-terminal region
Length = 739
Score = 30.3 bits (65), Expect = 1.4
Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 5/61 (8%)
Frame = -3
Query: 713 KLSSPIRTSSINLQ*PSFTLSGESNVDTISP-----PVTKPSLSIPSKSACSIAITPASA 549
K S+P +S P+F+ S S+ T +P T+ +L +PS S S A+ P +
Sbjct: 471 KTSTPASSSQPQTTSPAFSFSLPSSTSTTAPATSSATTTQTTLVVPSSSGTSTAVAPVAG 530
Query: 548 N 546
+
Sbjct: 531 S 531
>At1g71010.1 68414.m08192 phosphatidylinositol-4-phosphate 5-kinase
family protein low similarity to phosphatidylinositol
3,5-kinase [Candida albicans] GI:14571648; contains Pfam
profile PF01504: Phosphatidylinositol-4-phosphate
5-Kinase
Length = 1648
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/59 (23%), Positives = 28/59 (47%)
Frame = +1
Query: 505 LLNRQLIYNYPEQLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKL 681
LL + +Y +Q + + + + ++R+ TG + + DS +LGHC+L
Sbjct: 468 LLVEKSASSYAQQYLLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCEL 526
>At5g48300.1 68418.m05966 glucose-1-phosphate adenylyltransferase
small subunit 1 (APS1) / ADP-glucose pyrophosphorylase
(ADG1) identical to SP|P55228
Length = 520
Score = 29.1 bits (62), Expect = 3.3
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = +2
Query: 320 QPKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVAEKEK 460
Q + +A+I +A PMD + FG +K+D +I +E AEK K
Sbjct: 226 QAHRETDADITVAALPMDEQRATAFG-LMKIDEEGRI--IEFAEKPK 269
>At1g24510.2 68414.m03085 T-complex protein 1 epsilon subunit,
putative / TCP-1-epsilon, putative / chaperonin,
putative identical to SWISS-PROT:O04450- T-complex
protein 1, epsilon subunit (TCP-1-epsilon) [Arabidopsis
thaliana]; strong similarity to SP|P54411 T-complex
protein 1, epsilon subunit (TCP-1-epsilon) (CCT-epsilon)
(TCP-K36) {Avena sativa}; contains Pfam:PF00118 domain,
TCP-1/cpn60 chaperonin family
Length = 459
Score = 29.1 bits (62), Expect = 3.3
Identities = 21/65 (32%), Positives = 32/65 (49%)
Frame = +3
Query: 9 IIAGWRIASDAAKQALAEASFDHQKNLNEASLRVDLENIARTTLSSKILSNHKEHFTKLA 188
I G+ +AS A + L + + ++N L TTLSSKI++ K ++A
Sbjct: 53 IAEGYEMASRVAVEHLERIAQKFEFDVNNYE---PLVQTCMTTLSSKIVNRCKRSLAEIA 109
Query: 189 VDAVL 203
V AVL
Sbjct: 110 VKAVL 114
>At1g24510.1 68414.m03086 T-complex protein 1 epsilon subunit,
putative / TCP-1-epsilon, putative / chaperonin,
putative identical to SWISS-PROT:O04450- T-complex
protein 1, epsilon subunit (TCP-1-epsilon) [Arabidopsis
thaliana]; strong similarity to SP|P54411 T-complex
protein 1, epsilon subunit (TCP-1-epsilon) (CCT-epsilon)
(TCP-K36) {Avena sativa}; contains Pfam:PF00118 domain,
TCP-1/cpn60 chaperonin family
Length = 535
Score = 29.1 bits (62), Expect = 3.3
Identities = 21/65 (32%), Positives = 32/65 (49%)
Frame = +3
Query: 9 IIAGWRIASDAAKQALAEASFDHQKNLNEASLRVDLENIARTTLSSKILSNHKEHFTKLA 188
I G+ +AS A + L + + ++N L TTLSSKI++ K ++A
Sbjct: 129 IAEGYEMASRVAVEHLERIAQKFEFDVNNYE---PLVQTCMTTLSSKIVNRCKRSLAEIA 185
Query: 189 VDAVL 203
V AVL
Sbjct: 186 VKAVL 190
>At1g19700.1 68414.m02457 homeobox-leucine zipper family protein
similar to BEL1-like homeodomain 1 (GI:13877517)
[Arabidopsis thaliana]; similar to homeodomain protein
GI:7239157 from (Malus domestica); contains weak hit to
Pfam profile PF00046: Homeobox domain
Length = 538
Score = 29.1 bits (62), Expect = 3.3
Identities = 19/73 (26%), Positives = 38/73 (52%)
Frame = +2
Query: 269 ESFLDEGFLLNKKVGVHQPKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVA 448
++ LDE + K++ KK++ + + + +I+ G + DS K EL
Sbjct: 176 QNLLDEVVSVKKELNQMGKKKMK-----VNDFNSGSKEIEGGGGELSSDSNGKSIELSTI 230
Query: 449 EKEKMKDKVNKIL 487
E+E++++K NK+L
Sbjct: 231 EREELQNKKNKLL 243
>At4g33240.1 68417.m04731 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to SP|Q9Z1T6 FYVE
finger-containing phosphoinositide kinase (EC 2.7.1.68)
(1- phosphatidylinositol-4-phosphate kinase) (PIP5K)
(PtdIns(4)P-5-kinase) {Mus musculus}; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF01363: FYVE zinc finger, PF00118:
TCP-1/cpn60 chaperonin family
Length = 1756
Score = 28.7 bits (61), Expect = 4.4
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 595 IERLGLVTGGEIVSTFDSPDKVKLGHCKL 681
+ER+ TG +IV + D KLG+C L
Sbjct: 541 LERISRCTGAQIVPSIDQLTSPKLGYCDL 569
>At3g63150.1 68416.m07092 GTP-binding protein-related low similarity
to SP|Q38912 RAC-like GTP binding protein ARAC3
(GTP-binding protein ROP6) {Arabidopsis thaliana};
contains Pfam profile PF00036: EF hand (domain)
Length = 643
Score = 28.3 bits (60), Expect = 5.8
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -3
Query: 641 NVDTISPPVTKPSLSIPSKSACSIAITPASANN 543
NV + PP+T P+ + P +I TP+S +N
Sbjct: 42 NVPRVLPPITLPADAFPDYIPITIVDTPSSIDN 74
>At1g05800.1 68414.m00606 lipase class 3 family protein similar to
DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
GI:16215706; contains Pfam profile PF01764: Lipase
Length = 471
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = -3
Query: 632 TISPPVTKPSLSIPSKSACSIAITPASA 549
T+SPP++ LS+PS S+ S AI P+ A
Sbjct: 47 TMSPPISSSPLSLPSSSS-SQAIPPSRA 73
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,173,340
Number of Sequences: 28952
Number of extensions: 305126
Number of successful extensions: 932
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 888
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 930
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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