BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20927
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 29 0.46
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 29 0.81
SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces ... 27 2.5
SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pomb... 27 2.5
SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce... 26 5.7
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 26 5.7
SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 25 10.0
SPCC16C4.12 |||NatB N-acetyltransferase complex catalytic subuni... 25 10.0
SPAC31A2.14 |||WD repeat protein, human WRDR48 family|Schizosacc... 25 10.0
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 25 10.0
SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 25 10.0
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 29.5 bits (63), Expect = 0.46
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = +2
Query: 494 VDNKDRDRYSCRPIQTWTKKRSARPA*LG*SRLNAPSRCTATSESSS 634
V +KD DR+S + +++W + P +N+ S CTA SES S
Sbjct: 1765 VTDKDIDRFSLKMLESWGLFENKAPF------VNSTSICTAVSESRS 1805
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 28.7 bits (61), Expect = 0.81
Identities = 15/27 (55%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = +3
Query: 9 DPRTRGS-EPHARTPKELQRERLEAAK 86
D GS EP +T KEL+RER +AAK
Sbjct: 13 DSSAPGSGEPRPKTEKELERERQKAAK 39
>SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 761
Score = 27.1 bits (57), Expect = 2.5
Identities = 15/39 (38%), Positives = 22/39 (56%)
Frame = -2
Query: 669 SQLVIVGSGQPPLEDSDVAVHREGALSLLQPNHAGRAER 553
SQL + +GQP SD ++ A L QPNH+ +E+
Sbjct: 2 SQLYAL-NGQPLAGKSDDLANKLAAFGLSQPNHSSLSEQ 39
>SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 478
Score = 27.1 bits (57), Expect = 2.5
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +1
Query: 58 CSVRDWRRPNVASRRAHRCPSAVAL 132
C ++WRRP + A +CPS +A+
Sbjct: 189 CGRQNWRRPVNFASHAKQCPSFLAM 213
>SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1261
Score = 25.8 bits (54), Expect = 5.7
Identities = 14/48 (29%), Positives = 26/48 (54%)
Frame = -2
Query: 225 NDPLYEYLWPVSLESSRQ*IQMETTRTSSSYQSHCRRTSVSAAAGHVW 82
+DPL + L P SLESS I + + +++ + +R + S + H +
Sbjct: 153 SDPLLDTLTPNSLESSFASISLNSFTSANEFIQFLKRLASSKLSIHTF 200
>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1111
Score = 25.8 bits (54), Expect = 5.7
Identities = 13/42 (30%), Positives = 21/42 (50%), Gaps = 5/42 (11%)
Frame = +1
Query: 1 VRQTQEREAANRMRERLRSC-----SVRDWRRPNVASRRAHR 111
+ ++E +A + L+SC + RDW R A R+ HR
Sbjct: 825 IESSREAFSATLFKAALKSCFYDLQNARDWYREVTADRKMHR 866
>SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 383
Score = 25.0 bits (52), Expect = 10.0
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = -2
Query: 351 AGTGLPASCCAGDATGAVTSPSLRSRDTNESALCRGCRAS 232
AG G P CAG A A SPS S ++ +A R S
Sbjct: 150 AGNGNPN--CAGIAIEAFVSPSSSSSSSSSAATSTSTRVS 187
>SPCC16C4.12 |||NatB N-acetyltransferase complex catalytic subunit
Nat3 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 180
Score = 25.0 bits (52), Expect = 10.0
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = -1
Query: 193 VVGIFSAIDSDGDDEDIVELPKPLPKDI 110
V+G +S + G DED ++ KPL +D+
Sbjct: 134 VIGYYS--NPHGKDEDSFDMRKPLSRDV 159
>SPAC31A2.14 |||WD repeat protein, human WRDR48
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 962
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/50 (26%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
Frame = -2
Query: 321 AGDATGAVTSPSLRSRDTNES--ALCRGCRASWQNDPLYEYLWPVSLESS 178
AGD +G +T +R++ N+S A+C+ ++W S + S
Sbjct: 276 AGDRSGLITRTDIRNQPNNQSCTAICKQDAPVSDIVARQSFIWSTSRDGS 325
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 25.0 bits (52), Expect = 10.0
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = +2
Query: 251 HNADSFVSRLRSEGDVTAPVA 313
H D F+ L+SE D P+A
Sbjct: 437 HQLDEFIENLKSEFDYKGPIA 457
>SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 383
Score = 25.0 bits (52), Expect = 10.0
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = -2
Query: 351 AGTGLPASCCAGDATGAVTSPSLRSRDTNESALCRGCRAS 232
AG G P CAG A A SPS S ++ +A R S
Sbjct: 150 AGNGNPN--CAGIAIEAFVSPSSSSSSSSSAATSTSTRVS 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,432,388
Number of Sequences: 5004
Number of extensions: 44160
Number of successful extensions: 127
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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