Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20924
(809 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi... 63 5e-11
SPAC1142.02c ||SPAC17G6.19c|TPR repeat protein|Schizosaccharomyc... 51 2e-07
SPBC543.02c |||DNAJ/TPR domain protein DNAJC7 family|Schizosacch... 39 0.001
SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces... 34 0.027
SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces... 31 0.15
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 2.4
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 27 3.2
SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 26 5.5
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 26 7.3
SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccha... 26 7.3
SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|... 26 7.3
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 25 9.6
>SPAC6B12.12 |tom70||mitochondrial TOM complex subunit
Tom70|Schizosaccharomyces pombe|chr 1|||Manual
Length = 625
Score = 62.9 bits (146), Expect = 5e-11
Identities = 35/89 (39%), Positives = 50/89 (56%), Gaps = 2/89 (2%)
Frame = +1
Query: 517 ALKLNEKYVKAFLRRSRAAEKSGDLVLALEDVTSACILERFQVQSSLVNADRILKALGRQ 696
AL L+ YVKA RRS A E+ G L AL D T +CI + F +S +R+LK + +
Sbjct: 210 ALSLDSSYVKALNRRSAAYEQLGKLDEALMDSTVSCIFDGFANESMTATVERLLKKVAEK 269
Query: 697 HAREALAKRSPVMPSKHFIKTYF--LHSQ 777
+ L +R P +P+ FI+TY H+Q
Sbjct: 270 KSSALLKERPPKLPAASFIQTYLDSFHAQ 298
Score = 41.9 bits (94), Expect = 1e-04
Identities = 28/76 (36%), Positives = 39/76 (51%)
Frame = +2
Query: 281 EENTTKVSESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNR 460
E + E + A +LK GN+A+ EY AI Y +AI C D P+ F+ NR
Sbjct: 137 ESVKSMTKEERAKLAAELKTLGNKAYGQKEYANAIDYYTQAI-TCSHD-PI----FFSNR 190
Query: 461 SACYEKREMWEQVKED 508
+ACY +EQV +D
Sbjct: 191 AACYAAIGDFEQVIKD 206
>SPAC1142.02c ||SPAC17G6.19c|TPR repeat protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 317
Score = 51.2 bits (117), Expect = 2e-07
Identities = 33/78 (42%), Positives = 44/78 (56%)
Frame = +2
Query: 281 EENTTKVSESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNR 460
E++T V++ E A KLK GN A A +Y KA+ LY +AIE P PV +Y NR
Sbjct: 72 EDSTAHVNK---EEAEKLKLEGNNAIAAKDYQKALDLYTKAIE-IDPTSPV----YYSNR 123
Query: 461 SACYEKREMWEQVKEDAL 514
+A Y + +E EDAL
Sbjct: 124 AAAYNQLGQFENAVEDAL 141
>SPBC543.02c |||DNAJ/TPR domain protein DNAJC7
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 476
Score = 38.7 bits (86), Expect = 0.001
Identities = 22/82 (26%), Positives = 35/82 (42%)
Frame = +2
Query: 266 ISLDNEENTTKVSESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLAT 445
+ LD + T K + + K GN F G Y A Y+EA++ PD +A
Sbjct: 204 LKLDPDCTTAKTLFKQVRKLENTKNQGNDLFRQGNYQDAYEKYSEALQ-IDPDNKETVAK 262
Query: 446 FYQNRSACYEKREMWEQVKEDA 511
Y NR+ + + E+ D+
Sbjct: 263 LYMNRATVLLRLKRPEEALSDS 284
>SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 591
Score = 33.9 bits (74), Expect = 0.027
Identities = 21/63 (33%), Positives = 29/63 (46%)
Frame = +2
Query: 323 AMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQVK 502
A +LK GN AF +Y AI + +AI + Y NRSACY + +
Sbjct: 2 AEELKAKGNAAFSKKDYKTAIDYFTQAIGLDERNH-----ILYSNRSACYASEKDYADAL 56
Query: 503 EDA 511
+DA
Sbjct: 57 KDA 59
Score = 27.1 bits (57), Expect = 3.2
Identities = 16/54 (29%), Positives = 26/54 (48%)
Frame = +2
Query: 317 ERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEK 478
++A + + GN F +G++ AI Y E + P D + NR+A Y K
Sbjct: 397 DKAEESRVKGNELFKSGDFANAIKEYTEMTKRAPSD-----PRGFGNRAAAYLK 445
>SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 403
Score = 31.5 bits (68), Expect = 0.15
Identities = 13/34 (38%), Positives = 22/34 (64%)
Frame = +2
Query: 305 ESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAI 406
ES+ E A++ G + +AGEYDKA+ N+++
Sbjct: 2 ESSREEALRCIGLARKYLNAGEYDKALKFANKSL 35
>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 547
Score = 27.5 bits (58), Expect = 2.4
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +3
Query: 609 CNICMYP*EIPSSEFLSKC 665
C +C Y ++ SSEF SKC
Sbjct: 244 CPVCRYTQKVQSSEFQSKC 262
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 27.1 bits (57), Expect = 3.2
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = -2
Query: 655 RNSELGISQGYMQMLHLQEQAQDRHFF 575
+++ L I QGY+ ++HL +Q Q ++F
Sbjct: 408 QDNRLNIIQGYLDLVHLSDQDQYDNWF 434
>SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 26.2 bits (55), Expect = 5.5
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = -1
Query: 611 TSSRASTRSPLFSAARDRRRNAFTYFSLSFNAKVHLL 501
TSSR TRSPL S + R S +F+ +L
Sbjct: 468 TSSRKRTRSPLMSKGKSYDRKGSISMSKNFSPDCEIL 504
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.8 bits (54), Expect = 7.3
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +3
Query: 723 QPSHAFKTFYQNILSAFSEDPIA 791
+PS+ + + QN+LS +S P+A
Sbjct: 403 EPSNIYDEYEQNLLSPWSRSPVA 425
>SPAC8C9.06c |||mitochondrial translation regulator
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 931
Score = 25.8 bits (54), Expect = 7.3
Identities = 11/36 (30%), Positives = 20/36 (55%), Gaps = 3/36 (8%)
Frame = -3
Query: 756 FDKMF*RHDWAAFSKC---FSGMLSA*CFKNTISIY 658
FD+M+ H W++F+ C ++ F+ IS+Y
Sbjct: 271 FDEMYRTHHWSSFTACRLMIESLVRQNKFEEAISLY 306
>SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 402
Score = 25.8 bits (54), Expect = 7.3
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3
Query: 393 TMKQLKHAPQTVLSILLHFTKTALLVMKNE 482
T LK+A V + L HF ++ +V++NE
Sbjct: 111 TQNNLKYALSYVTNALFHFDNSSHMVIRNE 140
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 25.4 bits (53), Expect = 9.6
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -1
Query: 419 GGMLQLLHCIKQWLCHILQHEKPGYQHLLVSWRV 318
GGM +L +++WL I EKP L S+++
Sbjct: 609 GGMQELFSILEKWLLGIGLGEKPNISVLSESYKL 642
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,362,417
Number of Sequences: 5004
Number of extensions: 70984
Number of successful extensions: 217
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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