Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20920
(805 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|... 70 3e-13
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 68 2e-12
SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr 1|... 65 1e-11
SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces pom... 31 0.19
SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|S... 27 2.4
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 25 9.5
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 9.5
>SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr
1|||Manual
Length = 785
Score = 70.1 bits (164), Expect = 3e-13
Identities = 37/75 (49%), Positives = 47/75 (62%)
Frame = +3
Query: 228 FHTRQGCF*AFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELS 407
F + + F +YK LAKRLL +S S DAE M+S+LKQE G FT KLEGMF DM LS
Sbjct: 458 FISEKDVFEKYYKTHLAKRLLNNRSISSDAELGMISRLKQEAGNVFTQKLEGMFNDMNLS 517
Query: 408 KDINITYKQVNCTDS 452
+++ YK + S
Sbjct: 518 QELLQEYKHNSALQS 532
Score = 49.6 bits (113), Expect = 5e-07
Identities = 26/81 (32%), Positives = 45/81 (55%)
Frame = +1
Query: 13 EQLDHVVNTCFQRNDKFIYSMREAFEFFINQRQNKPAELIAKFVDLKLRAGNKEATEEEL 192
++L+ +++T + + S+ +AF F++ P E I+ F+D L+ ++A E +
Sbjct: 387 DRLNTIISTTMDADRSILNSLSDAFVTFVDGYTRAP-EYISLFIDDNLKKDARKAIEGSI 445
Query: 193 ERLLDKIMVLFRFIHGKDVFE 255
E L + LFRFI KDVFE
Sbjct: 446 EATLQNSVTLFRFISEKDVFE 466
>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 734
Score = 67.7 bits (158), Expect = 2e-12
Identities = 33/83 (39%), Positives = 50/83 (60%), Gaps = 2/83 (2%)
Frame = +3
Query: 249 F*AFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELSKDINITY 428
F A+YK D+AKRLL+ KSAS E +L LK+ CG FT LEGMF+D+ +SK+ ++
Sbjct: 423 FEAYYKLDIAKRLLLNKSASAQNELMLLDMLKKTCGSQFTHSLEGMFRDVNISKEFTSSF 482
Query: 429 KQVNCTDSRYR--IIGEFSMSYY 491
+ + +R + S +Y+
Sbjct: 483 RHSKAAHNLHRDLYVNVLSQAYW 505
Score = 66.9 bits (156), Expect = 3e-12
Identities = 36/93 (38%), Positives = 55/93 (59%), Gaps = 4/93 (4%)
Frame = +1
Query: 1 LDFKEQLDHVVNTCFQRNDKFIYSMREAFEFFIN----QRQNKPAELIAKFVDLKLRAGN 168
L F + L VV+ F ++ Y+MR+AFE FIN ++ PA LIAK++D LR G
Sbjct: 336 LSFHKFLQVVVDESFLHDETLSYAMRKAFETFINGAKGSQREAPARLIAKYIDYLLRVGE 395
Query: 169 KEATEEELERLLDKIMVLFRFIHGKDVFELSIK 267
+ + + L+ + +I+ LFR+I KD+FE K
Sbjct: 396 QASGGKPLKEVFSEILDLFRYIASKDIFEAYYK 428
>SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 767
Score = 64.9 bits (151), Expect = 1e-11
Identities = 33/60 (55%), Positives = 43/60 (71%)
Frame = +3
Query: 258 FYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELSKDINITYKQV 437
FY K LAKRL+ G S S DAE SMLSKLK+ CG +T KL+ MF+D+ LS++I + Q+
Sbjct: 460 FYTKLLAKRLVNGTSNSQDAESSMLSKLKEVCGFEYTSKLQRMFQDISLSQEITEAFWQL 519
Score = 52.8 bits (121), Expect = 5e-08
Identities = 27/82 (32%), Positives = 45/82 (54%), Gaps = 6/82 (7%)
Frame = +1
Query: 28 VVNTCFQRNDKFIYSMREAFEFFINQR------QNKPAELIAKFVDLKLRAGNKEATEEE 189
+VNT F + F S+ AF +N+ ++ EL+AK+ D LR NK ++
Sbjct: 377 LVNTAFHGDTDFTKSLDTAFRELVNRNVVCQRSSSRSPELLAKYADSILRKSNKNVDIDD 436
Query: 190 LERLLDKIMVLFRFIHGKDVFE 255
+E L I+++FR++ KDVF+
Sbjct: 437 VEDCLSSIIIIFRYVEDKDVFQ 458
>SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 264
Score = 31.1 bits (67), Expect = 0.19
Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
Frame = +3
Query: 357 GGFTCKLEGMFKDMELSK--DINITYKQVNCTD-SRYRIIGEFSMSYYILLLYNTTLHKV 527
G F ++ + E ++ ++N+TY +V C+ RYRI MSY +L+ Y+ H +
Sbjct: 176 GSFDLEVNDSLTNAEKNRKLNVNLTYNEVLCSMLQRYRIDPNTWMSYDLLINYDDKEHAI 235
>SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor
Raf1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 638
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -1
Query: 631 IHCYLTKNKL*EYYLCIFYNSLCSFFSKENIH 536
+ Y KL YL FYN+ S+F +E +H
Sbjct: 188 LRTYKKNKKLLPDYLKSFYNAGSSYFQREQVH 219
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 25.4 bits (53), Expect = 9.5
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = +1
Query: 124 ELIAKFVDLKLRA--GNKEATEEELERLLDKIMVLFRFIH 237
+++ +F +++LR KE ELE+ D+ RFIH
Sbjct: 1046 DILTEFYEVRLRTYQRRKEHMVNELEKRFDRFSNQARFIH 1085
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 562 SFFSKENIHIDITLCNVVLYSSSI 491
SFF+K H D T N V++++S+
Sbjct: 1996 SFFTKSITHFDATDANSVMFANSL 2019
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,379,004
Number of Sequences: 5004
Number of extensions: 69498
Number of successful extensions: 154
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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