Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20920
(805 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619 C... 110 1e-24
At1g26830.1 68414.m03270 cullin, putative similar to Cullin homo... 78 8e-15
At1g69670.1 68414.m08018 cullin, putative contains similarity to... 77 1e-14
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 67 1e-11
At1g02980.1 68414.m00268 cullin family protein similar to cullin... 51 1e-06
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 32 0.51
At3g44500.1 68416.m04783 Ulp1 protease family protein similar to... 29 2.7
At5g41770.1 68418.m05086 crooked neck protein, putative / cell c... 29 4.8
At3g13210.1 68416.m01653 crooked neck protein, putative / cell c... 29 4.8
At5g45990.1 68418.m05656 crooked neck protein, putative / cell c... 28 6.3
At4g04010.1 68417.m00571 Ulp1 protease family protein contains P... 28 6.3
At3g47260.1 68416.m05133 Ulp1 protease family protein contains P... 28 6.3
At1g32840.1 68414.m04047 Ulp1 protease family protein similar to... 28 6.3
>At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619
Cullin homolog 4A (CUL-4A) {Homo sapiens}; contains Pfam
profile PF00888: Cullin family
Length = 792
Score = 110 bits (264), Expect = 1e-24
Identities = 52/90 (57%), Positives = 68/90 (75%)
Frame = +1
Query: 1 LDFKEQLDHVVNTCFQRNDKFIYSMREAFEFFINQRQNKPAELIAKFVDLKLRAGNKEAT 180
LDFK LD + F +N+ F +++++FE IN RQN+PAELIAKF+D KLRAGNK +
Sbjct: 398 LDFKASLDIIWEESFYKNESFGNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 457
Query: 181 EEELERLLDKIMVLFRFIHGKDVFELSIKR 270
EEELE +L+K++VLFRFI GKDVFE K+
Sbjct: 458 EEELESVLEKVLVLFRFIQGKDVFEAFYKK 487
Score = 105 bits (253), Expect = 3e-23
Identities = 53/83 (63%), Positives = 66/83 (79%)
Frame = +3
Query: 249 F*AFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELSKDINITY 428
F AFYKKDLAKRLL+GKSAS+DAEKSM+SKLK ECG FT KLEGMFKD+ELSK+IN ++
Sbjct: 481 FEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESF 540
Query: 429 KQVNCTDSRYRIIGEFSMSYYIL 497
KQ + +R ++ MS ++L
Sbjct: 541 KQ--SSQARTKLPSGIEMSVHVL 561
>At1g26830.1 68414.m03270 cullin, putative similar to Cullin homolog
3 (CUL-3) SP:Q13618, GI:3639052 from [Homo sapiens];
contains Pfam profile PF00888: Cullin family
Length = 732
Score = 77.8 bits (183), Expect = 8e-15
Identities = 38/90 (42%), Positives = 58/90 (64%)
Frame = +1
Query: 1 LDFKEQLDHVVNTCFQRNDKFIYSMREAFEFFINQRQNKPAELIAKFVDLKLRAGNKEAT 180
LD +++ D ++NT F + F ++ +FE+FIN P E I+ FVD KLR G K T
Sbjct: 337 LDERDKYDKIINTAFGNDKTFQNALNSSFEYFINLNARSP-EFISLFVDDKLRKGLKGIT 395
Query: 181 EEELERLLDKIMVLFRFIHGKDVFELSIKR 270
+ ++E +LDK+M+LFR++ KDVFE K+
Sbjct: 396 DVDVEVILDKVMMLFRYLQEKDVFEKYYKQ 425
Score = 70.9 bits (166), Expect = 9e-13
Identities = 34/55 (61%), Positives = 42/55 (76%)
Frame = +3
Query: 249 F*AFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELSKD 413
F +YK+ LAKRLL GK+ S DAE+S++ KLK ECG FT KLEGMF DM+ S+D
Sbjct: 419 FEKYYKQHLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGMFTDMKTSED 473
>At1g69670.1 68414.m08018 cullin, putative contains similarity to
Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from
[Homo sapiens]; contains Pfam profile PF00888: Cullin
family
Length = 732
Score = 77.0 bits (181), Expect = 1e-14
Identities = 36/90 (40%), Positives = 57/90 (63%)
Frame = +1
Query: 1 LDFKEQLDHVVNTCFQRNDKFIYSMREAFEFFINQRQNKPAELIAKFVDLKLRAGNKEAT 180
LD +++ D ++N F + F ++ +FE+F+N P E I+ FVD KLR G K
Sbjct: 337 LDERDKYDRIINMAFNNDKTFQNALNSSFEYFVNLNTRSP-EFISLFVDDKLRKGLKGVG 395
Query: 181 EEELERLLDKIMVLFRFIHGKDVFELSIKR 270
EE+++ +LDK+M+LFR++ KDVFE K+
Sbjct: 396 EEDVDLILDKVMMLFRYLQEKDVFEKYYKQ 425
Score = 68.9 bits (161), Expect = 4e-12
Identities = 33/55 (60%), Positives = 41/55 (74%)
Frame = +3
Query: 249 F*AFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELSKD 413
F +YK+ LAKRLL GK+ S DAE++++ KLK ECG FT KLEGMF DM+ S D
Sbjct: 419 FEKYYKQHLAKRLLSGKTVSDDAERNLIVKLKTECGYQFTSKLEGMFTDMKTSHD 473
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile
PF00888: Cullin family
Length = 738
Score = 67.3 bits (157), Expect = 1e-11
Identities = 30/55 (54%), Positives = 43/55 (78%)
Frame = +3
Query: 249 F*AFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELSKD 413
F FY+K LA+RLL +SA+ D E+S+L+KLKQ+CGG FT K+EGM D+ L+++
Sbjct: 429 FAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARE 483
Score = 52.4 bits (120), Expect = 3e-07
Identities = 29/77 (37%), Positives = 45/77 (58%), Gaps = 3/77 (3%)
Frame = +1
Query: 31 VNTCFQRNDKFIYSMREAFEFFINQ--RQNKPAELIAKFVDLKLRAGNKE-ATEEELERL 201
V CFQ + F +++EAFE F N+ + AEL+A F D L+ G E ++E +E
Sbjct: 353 VTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDT 412
Query: 202 LDKIMVLFRFIHGKDVF 252
L+K++ L +I KD+F
Sbjct: 413 LEKVVKLLAYISDKDLF 429
>At1g02980.1 68414.m00268 cullin family protein similar to cullin 1
[Homo sapiens] GI:3139077; contains Pfam profile
PF00888: Cullin family
Length = 742
Score = 50.8 bits (116), Expect = 1e-06
Identities = 29/88 (32%), Positives = 50/88 (56%), Gaps = 4/88 (4%)
Frame = +1
Query: 1 LDFKEQLDHVVNTCFQRNDKFIYSMREAFEFFINQRQN--KPAELIAKFVD--LKLRAGN 168
+D ++ V+ CFQ++ F +++EAFE F N+ AE++A + D LK G
Sbjct: 346 IDLHDKFMVYVDECFQKHSLFHKALKEAFEVFCNKTVAGVSSAEILATYCDNILKTGGGI 405
Query: 169 KEATEEELERLLDKIMVLFRFIHGKDVF 252
++ E+LE L+K++ L +I KD+F
Sbjct: 406 EKLENEDLELTLEKVVKLLVYISDKDLF 433
Score = 48.8 bits (111), Expect = 4e-06
Identities = 23/55 (41%), Positives = 36/55 (65%)
Frame = +3
Query: 249 F*AFYKKDLAKRLLVGKSASVDAEKSMLSKLKQECGGGFTCKLEGMFKDMELSKD 413
F F++K A+RLL ++ + E+S+L+K K+ G FT K+EGM DM L+K+
Sbjct: 433 FAEFFRKKQARRLLFDRNGNDYHERSLLTKFKELLGAQFTSKMEGMLTDMTLAKE 487
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3)
{Arabidopsis thaliana}
Length = 1473
Score = 31.9 bits (69), Expect = 0.51
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +1
Query: 118 PAELIAKFVDLKLRAGNKEATEEELERLLDKI 213
P + +VDL L A NK TE+ L RL +K+
Sbjct: 265 PVKSFTDYVDLYLSAANKSRTEDPLPRLTEKV 296
>At3g44500.1 68416.m04783 Ulp1 protease family protein similar to
At1g32840, At4g04010, At2g06430, At2g15140, At2g04980,
At2g14130, At2g15190, At3g47260, At5g34900, At3g29210,
At2g02210, At3g32900
Length = 805
Score = 29.5 bits (63), Expect = 2.7
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +1
Query: 112 NKPAELIAKFVDLKLRAGNKEATEEELER 198
NK ELI K +D+++ N + +EE+ ER
Sbjct: 359 NKRVELIEKILDIRMEEKNNQRSEEDEER 387
>At5g41770.1 68418.m05086 crooked neck protein, putative / cell
cycle protein, putative similar to Swiss-Prot:P17886
crooked neck protein [Drosophila melanogaster]
Length = 705
Score = 28.7 bits (61), Expect = 4.8
Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +1
Query: 76 REAFEFFINQRQNKPAELIAK-FVDLKLRAGNKEATEEELERLLDK 210
R FE I+Q EL+ K ++D ++ G E T ERLLD+
Sbjct: 510 RAIFELAISQPALDMPELLWKAYIDFEISEGELERTRALYERLLDR 555
>At3g13210.1 68416.m01653 crooked neck protein, putative / cell
cycle protein, putative similar to Swiss-Prot:P17886
crooked neck protein [Drosophila melanogaster]
Length = 657
Score = 28.7 bits (61), Expect = 4.8
Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +1
Query: 76 REAFEFFINQRQNKPAELIAK-FVDLKLRAGNKEATEEELERLLDK 210
R FE I+Q EL+ K ++D ++ G E T ERLLD+
Sbjct: 467 RAIFELAISQPALDMPELLWKTYIDFEISEGELERTRALYERLLDR 512
>At5g45990.1 68418.m05656 crooked neck protein, putative / cell
cycle protein, putative similar to Swiss-Prot:P17886
crooked neck protein [Drosophila melanogaster]
Length = 673
Score = 28.3 bits (60), Expect = 6.3
Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +1
Query: 76 REAFEFFINQRQNKPAELIAK-FVDLKLRAGNKEATEEELERLLDK 210
R FE I+Q EL+ K ++D ++ G E T ERLLD+
Sbjct: 497 RAIFELAISQPALDMPELLWKTYIDFEISEGEFEKTRALYERLLDR 542
>At4g04010.1 68417.m00571 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g32840, At2g06430,
At2g15140, At2g04980, At2g14130, At3g44500, At2g15190,
At3g47260, At5g34900, At3g29210, At2g02210, At3g32900
Length = 836
Score = 28.3 bits (60), Expect = 6.3
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = +1
Query: 112 NKPAELIAKFVDLKLRAGNKEATEEELER 198
NK ELI + +D+++ N + +EE+ ER
Sbjct: 363 NKRVELIERILDIRMEEKNNQRSEEDEER 391
>At3g47260.1 68416.m05133 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At1g32840, At4g04010,
At2g06430, At2g15140, At2g04980, At2g14130, At3g44500,
At2g15190, At5g34900, At3g29210, At2g02210, At3g32900
Length = 820
Score = 28.3 bits (60), Expect = 6.3
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = +1
Query: 112 NKPAELIAKFVDLKLRAGNKEATEEELER 198
NK ELI + +D+++ N + +EE+ ER
Sbjct: 391 NKRVELIERILDIRMEEKNNQRSEEDEER 419
>At1g32840.1 68414.m04047 Ulp1 protease family protein similar to
At4g04010, At2g06430, At2g15140, At2g04980, At2g14130,
At3g44500, At2g15190, At3g47260, At5g34900, At3g29210,
At2g02210, At3g32900
Length = 611
Score = 28.3 bits (60), Expect = 6.3
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = +1
Query: 112 NKPAELIAKFVDLKLRAGNKEATEEELER 198
NK ELI + +D+++ N + +EE+ ER
Sbjct: 157 NKRVELIERILDIRMEEKNNQRSEEDEER 185
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,242,685
Number of Sequences: 28952
Number of extensions: 349271
Number of successful extensions: 727
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 725
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1824072800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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