Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20918
(690 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 123 2e-29
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 81 1e-16
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 80 3e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 76 4e-15
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 1.1
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 28 1.1
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.5
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 26 5.9
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 26 5.9
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 123 bits (297), Expect = 2e-29
Identities = 52/67 (77%), Positives = 61/67 (91%)
Frame = +3
Query: 255 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 434
LEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 435 DCLQGFQ 455
D LQGFQ
Sbjct: 128 DALQGFQ 134
Score = 120 bits (290), Expect = 1e-28
Identities = 50/70 (71%), Positives = 59/70 (84%)
Frame = +1
Query: 55 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 234
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 235 PRAILVDWSP 264
PRA+LVD P
Sbjct: 61 PRAVLVDLEP 70
Score = 104 bits (249), Expect = 1e-23
Identities = 47/58 (81%), Positives = 53/58 (91%)
Frame = +2
Query: 509 SKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI 682
SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I
Sbjct: 153 SKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSI 210
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 81.0 bits (191), Expect = 1e-16
Identities = 32/66 (48%), Positives = 48/66 (72%)
Frame = +3
Query: 255 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 434
LEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++C
Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133
Query: 435 DCLQGF 452
LQGF
Sbjct: 134 SGLQGF 139
Score = 65.3 bits (152), Expect = 8e-12
Identities = 27/58 (46%), Positives = 41/58 (70%)
Frame = +2
Query: 512 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 685
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217
Score = 56.0 bits (129), Expect = 5e-09
Identities = 28/76 (36%), Positives = 40/76 (52%), Gaps = 6/76 (7%)
Frame = +1
Query: 55 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 216
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 217 SGGKYVPRAILVDWSP 264
GK+VPR+I VD P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 79.8 bits (188), Expect = 3e-16
Identities = 32/66 (48%), Positives = 46/66 (69%)
Frame = +3
Query: 255 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 434
LEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++C
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129
Query: 435 DCLQGF 452
LQGF
Sbjct: 130 SGLQGF 135
Score = 62.1 bits (144), Expect = 7e-11
Identities = 30/72 (41%), Positives = 41/72 (56%), Gaps = 2/72 (2%)
Frame = +1
Query: 55 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 228
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 229 YVPRAILVDWSP 264
YVPR+I VD P
Sbjct: 61 YVPRSIYVDLEP 72
Score = 60.5 bits (140), Expect = 2e-10
Identities = 25/58 (43%), Positives = 39/58 (67%)
Frame = +2
Query: 512 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 685
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDIC 213
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 76.2 bits (179), Expect = 4e-15
Identities = 32/69 (46%), Positives = 48/69 (69%)
Frame = +1
Query: 58 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 237
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 238 RAILVDWSP 264
RAIL+D P
Sbjct: 63 RAILIDLEP 71
Score = 56.4 bits (130), Expect = 4e-09
Identities = 22/68 (32%), Positives = 44/68 (64%), Gaps = 2/68 (2%)
Frame = +3
Query: 255 LEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKESE 428
LEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E++
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 429 SCDCLQGF 452
D L+GF
Sbjct: 128 GSDSLEGF 135
Score = 55.2 bits (127), Expect = 8e-09
Identities = 26/58 (44%), Positives = 37/58 (63%), Gaps = 1/58 (1%)
Frame = +2
Query: 512 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI 682
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 28.3 bits (60), Expect = 1.1
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +1
Query: 454 NLHIPSVAAPGPVWAPSSFKDP*RVPRQNHEHILSSPLAQS 576
N+H PS A + ++P +P+QNHEH SPL +S
Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVRS 660
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 28.3 bits (60), Expect = 1.1
Identities = 24/69 (34%), Positives = 37/69 (53%), Gaps = 11/69 (15%)
Frame = +2
Query: 488 RYGHPPHSKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSVHQL---VE 634
+Y H SK++ EYP D+++ SV+ PK VVE P N A L+ H + ++
Sbjct: 392 QYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAAHSIEKGLD 451
Query: 635 NTDETYCID 661
N ++ Y ID
Sbjct: 452 NKEKFYGID 460
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 357 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 446
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 94 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 195
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 288 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 389
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 38 KITYTK*GKSFIYRPANVV 94
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/46 (26%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = +2
Query: 467 PRWRHRVRYGHPPHSKIREEYP-DRIMNTYSVVPSPKVSDTVVEPY 601
P + Y H HS E+ + + S++P P+VSD +++ +
Sbjct: 223 PSFSELSAYSHTHHSYPPNEHDVNAVQKALSLLPPPEVSDFLIKSF 268
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 25.8 bits (54), Expect = 5.9
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = -2
Query: 341 AGLSEDEVVRTEDLSERSGADRVHGAGLQSTRMARG 234
AG++ED + T + + +G++R L+ +R+ G
Sbjct: 279 AGINEDHTLATLSIIDLAGSERATATKLRGSRLFEG 314
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 309 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 440
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,696,115
Number of Sequences: 5004
Number of extensions: 54100
Number of successful extensions: 181
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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