BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20906 (649 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g03110.1 68416.m00307 exportin 1, putative strong similarity ... 79 3e-15 At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to Ex... 77 8e-15 At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 34 0.094 At5g53180.1 68418.m06611 polypyrimidine tract-binding protein, p... 28 4.7 At5g63190.2 68418.m07934 MA3 domain-containing protein low simil... 28 6.1 At5g63190.1 68418.m07933 MA3 domain-containing protein low simil... 28 6.1 >At3g03110.1 68416.m00307 exportin 1, putative strong similarity to Exportin1 (XPO1) protein [Arabidopsis thaliana] GI:7671510; contains Pfam profile PF03810: Importin-beta N-terminal domain Length = 1076 Score = 79.0 bits (186), Expect = 3e-15 Identities = 38/96 (39%), Positives = 59/96 (61%) Frame = +1 Query: 256 DILTALKEHPDAWTRVDTILEYSQNQETKYYALQILEQVILTRWKILPRNQCEGIKKYIV 435 +IL LK +PD W +V IL+ + + TK++ALQ+LE VI RW LP Q +G+K YI Sbjct: 39 NILRDLKANPDTWLQVVHILQNTSSTHTKFFALQVLEGVIKYRWNALPVEQRDGMKNYIS 98 Query: 436 GLIIKNSSDPVTMESNKVYLKKLNRSLFKF*KENGP 543 +I++ S D + + ++Y+ KLN L + K+ P Sbjct: 99 DVIVQLSRDEASFRTERLYVNKLNIILVQIVKQEWP 134 Score = 61.7 bits (143), Expect = 4e-10 Identities = 26/48 (54%), Positives = 37/48 (77%) Frame = +3 Query: 498 KTKQILIQVLKREWPHNWETFISDIVGASKTNESLCXNNMEIFKLLSE 641 K IL+Q++K+EWP W++FI D+V A+KT+E++C N M I KLLSE Sbjct: 120 KLNIILVQIVKQEWPAKWKSFIPDLVIAAKTSETICENCMAILKLLSE 167 >At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to Exportin1 (XPO1) protein [Arabidopsis thaliana] GI:7671510 Length = 1075 Score = 77.4 bits (182), Expect = 8e-15 Identities = 38/95 (40%), Positives = 60/95 (63%) Frame = +1 Query: 259 ILTALKEHPDAWTRVDTILEYSQNQETKYYALQILEQVILTRWKILPRNQCEGIKKYIVG 438 IL L+ +PD W +V IL+ + + +TK++ALQ+LE VI RW LP Q +G+K YI Sbjct: 40 ILRDLQANPDMWLQVVHILQNTNSLDTKFFALQVLEGVIKYRWNALPVEQRDGMKNYISE 99 Query: 439 LIIKNSSDPVTMESNKVYLKKLNRSLFKF*KENGP 543 +I++ SS+ + S ++Y+ KLN L + K + P Sbjct: 100 VIVQLSSNEASFRSERLYVNKLNVILVQIVKHDWP 134 Score = 61.3 bits (142), Expect = 5e-10 Identities = 25/48 (52%), Positives = 35/48 (72%) Frame = +3 Query: 498 KTKQILIQVLKREWPHNWETFISDIVGASKTNESLCXNNMEIFKLLSE 641 K IL+Q++K +WP W +FI D+V A+KT+E++C N M I KLLSE Sbjct: 120 KLNVILVQIVKHDWPAKWTSFIPDLVAAAKTSETICENCMAILKLLSE 167 >At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-) {Saccharomyces cerevisiae}; contains Pfam profiles PF00240: Ubiquitin family, PF00632: HECT-domain (ubiquitin-transferase) Length = 873 Score = 33.9 bits (74), Expect = 0.094 Identities = 26/80 (32%), Positives = 41/80 (51%), Gaps = 1/80 (1%) Frame = +1 Query: 277 EHPDAWTRVDTILEYSQNQETKYYALQI-LEQVILTRWKILPRNQCEGIKKYIVGLIIKN 453 EHP AW +D I+ Y+ ++ K LQ + + I+T + ++P E I KY+ I N Sbjct: 172 EHPVAWQTIDDIM-YTISRMYKGENLQSNINEKIVTFFAMIPVESDESIAKYL--NIFSN 228 Query: 454 SSDPVTMESNKVYLKKLNRS 513 SS P + +Y L R+ Sbjct: 229 SSVPAALV--MLYASSLERN 246 >At5g53180.1 68418.m06611 polypyrimidine tract-binding protein, putative / heterogeneous nuclear ribonucleoprotein, putative similar to Polypyrimidine tract-binding protein 1 (PTB) (Heterogeneous nuclear ribonucleoprotein I) (hnRNP I) from {Rattus norvegicus} SP|Q00438, {Homo sapiens} SP|P26599; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 429 Score = 28.3 bits (60), Expect = 4.7 Identities = 17/55 (30%), Positives = 27/55 (49%) Frame = +1 Query: 451 NSSDPVTMESNKVYLKKLNRSLFKF*KENGPIIGKLLSVTLWVPQRRMKVSVXTI 615 +SS+P + VYL+ NR K ++G +L VT+ RM VS+ + Sbjct: 77 SSSEPAQVRGKTVYLQYSNRQEIVNNKTTADVVGNVLLVTIEGDDARM-VSIDVL 130 >At5g63190.2 68418.m07934 MA3 domain-containing protein low similarity to programmed cell death 4 protein [Gallus gallus] GI:12958564; contains Pfam profile PF02847: MA3 domain Length = 702 Score = 27.9 bits (59), Expect = 6.1 Identities = 13/47 (27%), Positives = 26/47 (55%) Frame = +3 Query: 420 KKIYSWPYH*KFIRSCYDGKQQSLFEKTKQILIQVLKREWPHNWETF 560 K S P+H + + + G + E+TK+ + ++LK E+ N +T+ Sbjct: 260 KSYLSAPHHAELVEKKWGGSTHTTVEETKKKISEILK-EYVENGDTY 305 >At5g63190.1 68418.m07933 MA3 domain-containing protein low similarity to programmed cell death 4 protein [Gallus gallus] GI:12958564; contains Pfam profile PF02847: MA3 domain Length = 702 Score = 27.9 bits (59), Expect = 6.1 Identities = 13/47 (27%), Positives = 26/47 (55%) Frame = +3 Query: 420 KKIYSWPYH*KFIRSCYDGKQQSLFEKTKQILIQVLKREWPHNWETF 560 K S P+H + + + G + E+TK+ + ++LK E+ N +T+ Sbjct: 260 KSYLSAPHHAELVEKKWGGSTHTTVEETKKKISEILK-EYVENGDTY 305 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,989,875 Number of Sequences: 28952 Number of extensions: 249060 Number of successful extensions: 508 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 502 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 508 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1344285648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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