Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20896
(697 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 108 6e-25
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 104 1e-23
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 31 0.12
SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr ... 28 1.1
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 28 1.5
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 2.6
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 27 2.6
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 27 3.4
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 4.5
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 7.8
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 108 bits (260), Expect = 6e-25
Identities = 49/65 (75%), Positives = 59/65 (90%)
Frame = +2
Query: 503 ESYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTL 682
E Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L
Sbjct: 152 EGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSL 211
Query: 683 NEDSY 697
+E+SY
Sbjct: 212 SEESY 216
Score = 105 bits (251), Expect = 8e-24
Identities = 53/104 (50%), Positives = 69/104 (66%), Gaps = 2/104 (1%)
Frame = +3
Query: 255 SIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM 428
SIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVFY KM
Sbjct: 67 SIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKM 126
Query: 429 KGDYYRYLAEVATGETRHSVVEDSQKATKMLLKSARRKCSPHTP 560
KGDYYRYLAE A GE R + S + K + A + +P P
Sbjct: 127 KGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHP 170
Score = 87.0 bits (206), Expect = 2e-18
Identities = 40/69 (57%), Positives = 52/69 (75%)
Frame = +1
Query: 64 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVSYKNVVGARRSSW 243
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSV+YKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 244 RVISQLNRK 270
R++S + +K
Sbjct: 63 RIVSSIEQK 71
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 104 bits (250), Expect = 1e-23
Identities = 54/104 (51%), Positives = 66/104 (63%), Gaps = 2/104 (1%)
Frame = +3
Query: 255 SIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM 428
SIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKVFY KM
Sbjct: 66 SIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYKM 125
Query: 429 KGDYYRYLAEVATGETRHSVVEDSQKATKMLLKSARRKCSPHTP 560
KGDYYRYLAE GE + S +A K A + P P
Sbjct: 126 KGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDP 169
Score = 97.1 bits (231), Expect = 2e-21
Identities = 43/65 (66%), Positives = 57/65 (87%)
Frame = +2
Query: 503 ESYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTL 682
E+Y+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L
Sbjct: 151 EAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSL 210
Query: 683 NEDSY 697
+E+SY
Sbjct: 211 SEESY 215
Score = 85.8 bits (203), Expect = 5e-18
Identities = 41/70 (58%), Positives = 54/70 (77%)
Frame = +1
Query: 61 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVSYKNVVGARRSS 240
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSV+YKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 241 WRVISQLNRK 270
WR+IS + +K
Sbjct: 61 WRIISSIEQK 70
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 31.5 bits (68), Expect = 0.12
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 3/67 (4%)
Frame = +1
Query: 64 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVSYKNVVGARR 234
SV + ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V
Sbjct: 3 SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61
Query: 235 SSWRVIS 255
S+W S
Sbjct: 62 STWATFS 68
>SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 266
Score = 28.3 bits (60), Expect = 1.1
Identities = 23/75 (30%), Positives = 35/75 (46%)
Frame = +1
Query: 73 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVSYKNVVGARRSSWRVI 252
K E+ R L + E D + +++ ET +L E NL + +NV S I
Sbjct: 110 KAEIYNRDALNTKQEHLD-IKKRLEKSDETVCKLKEENENLQDM-LRNVGNELVESRDEI 167
Query: 253 SQLNRKPRVQKENSK 297
+L K +VQKE+ K
Sbjct: 168 KELIEKQKVQKESVK 182
>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 27.9 bits (59), Expect = 1.5
Identities = 9/41 (21%), Positives = 21/41 (51%)
Frame = -3
Query: 689 LRSMCPVRRWHRRKPVWRVDTPCLANLISHNKRLRNLTPDP 567
LR + P+ +W R++ +W + P + + ++ + P P
Sbjct: 119 LRDVTPINQWKRKRSLWDIKPPGYELVTADQAKMSGVFPLP 159
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 2.6
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +3
Query: 363 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 500
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 27.1 bits (57), Expect = 2.6
Identities = 28/92 (30%), Positives = 46/92 (50%), Gaps = 5/92 (5%)
Frame = +3
Query: 282 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNP---ESKVFYLKMKGDYYR 446
E++Q A +YR+KVE+ +I V + L+ L + SNP E V +++G
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPEKHEDAVSQNRLRGS--- 141
Query: 447 YLAEVATGETRHSVVEDSQKATKMLLKSARRK 542
L V++ H +D +KAT++ L A K
Sbjct: 142 -LDTVSSPSKTHKANKD-EKATRLHLIIANLK 171
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 3.4
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
Frame = +3
Query: 261 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 437
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 438 YYR-YLAEV 461
+R Y+ E+
Sbjct: 100 SFRDYVGEL 108
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 264 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 389
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 7.8
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +2
Query: 8 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 130
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,881,813
Number of Sequences: 5004
Number of extensions: 57628
Number of successful extensions: 210
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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