Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20896
(697 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 105 5e-23
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 101 7e-22
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 99 3e-21
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 93 1e-19
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) 72 4e-13
SB_3265| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 0.51
SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.6
SB_32481| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.6
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0) 28 6.3
>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 309
Score = 105 bits (251), Expect = 5e-23
Identities = 51/85 (60%), Positives = 60/85 (70%)
Frame = +3
Query: 255 SIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKG 434
SIEQK EGSERK+Q + YR +E EL E+C VL LL+ LIP A + ESKVFYLKMKG
Sbjct: 43 SIEQKLEGSERKKQNTETYRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKG 102
Query: 435 DYYRYLAEVATGETRHSVVEDSQKA 509
DYYRY EVA + R VV+ + KA
Sbjct: 103 DYYRYEGEVAGADRRREVVQKAMKA 127
Score = 91.9 bits (218), Expect = 5e-19
Identities = 42/62 (67%), Positives = 51/62 (82%)
Frame = +2
Query: 512 QDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNED 691
++A E K+ PT PIRLGLALNFSVF+YEI + ++AC+LAKQAFDDAIAELD+LNED
Sbjct: 220 KEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAFDDAIAELDSLNED 279
Query: 692 SY 697
Y
Sbjct: 280 QY 281
Score = 91.5 bits (217), Expect = 6e-19
Identities = 44/67 (65%), Positives = 54/67 (80%), Gaps = 2/67 (2%)
Frame = +2
Query: 503 ESYQDAFEISKA--KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELD 676
++Y +A EI++ K+ PT PIRLGLALNFSVFYYEI+ +AC LAK+AFDDAIAELD
Sbjct: 126 KAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAELD 185
Query: 677 TLNEDSY 697
TL+ED Y
Sbjct: 186 TLSEDQY 192
Score = 69.3 bits (162), Expect = 3e-12
Identities = 33/47 (70%), Positives = 37/47 (78%)
Frame = +1
Query: 130 MAAAMKEVTETGVELSNEERNLLSVSYKNVVGARRSSWRVISQLNRK 270
MA AMKE TE L EERNLLSV+YKNVVGA+RSSWRVIS + +K
Sbjct: 1 MAKAMKEATEISETLEQEERNLLSVAYKNVVGAKRSSWRVISSIEQK 47
>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
Length = 251
Score = 101 bits (241), Expect = 7e-22
Identities = 47/74 (63%), Positives = 59/74 (79%)
Frame = +1
Query: 70 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVSYKNVVGARRSSWRV 249
DKEE V AKLAEQAERYDDM +MKEV + G ELS E+RNLLSV+YKNV+GARR+SWR+
Sbjct: 3 DKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVAYKNVIGARRASWRI 62
Query: 250 ISQLNRKPRVQKEN 291
I+ + +K + E+
Sbjct: 63 ITSIEQKEESKGED 76
Score = 53.2 bits (122), Expect = 2e-07
Identities = 28/61 (45%), Positives = 38/61 (62%), Gaps = 3/61 (4%)
Frame = +3
Query: 255 SIEQKTEGSER---KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLK 425
SIEQK E K +M + YR +E+EL+ IC ++L LLD LI + + ESKVFY K
Sbjct: 65 SIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEESKVFYNK 124
Query: 426 M 428
+
Sbjct: 125 I 125
>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
Length = 248
Score = 99.1 bits (236), Expect = 3e-21
Identities = 47/74 (63%), Positives = 57/74 (77%)
Frame = +1
Query: 49 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVSYKNVVGA 228
P+ +EEL+ AK+AEQAERYDDM AM VT+ G L++EERNLLSV+YKNVVGA
Sbjct: 3 PNFVSKCSREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVAYKNVVGA 62
Query: 229 RRSSWRVISQLNRK 270
RRSSWRVIS + +K
Sbjct: 63 RRSSWRVISSMEQK 76
Score = 91.1 bits (216), Expect = 8e-19
Identities = 42/65 (64%), Positives = 52/65 (80%)
Frame = +2
Query: 503 ESYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTL 682
E+Y+DA ++ P+HPIRLGLALNFSVFYYEI N P +AC+LAK+AFDDAIA LD L
Sbjct: 156 EAYKDA-SAKAEELSPSHPIRLGLALNFSVFYYEIENKPPEACKLAKEAFDDAIAVLDNL 214
Query: 683 NEDSY 697
++SY
Sbjct: 215 KDESY 219
Score = 74.5 bits (175), Expect = 7e-14
Identities = 43/105 (40%), Positives = 61/105 (58%), Gaps = 3/105 (2%)
Frame = +3
Query: 255 SIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKAS---NPESKVFYLK 425
S+EQK E + K+YR + EL C +VL +L+ +L+ N E+KVFYLK
Sbjct: 72 SMEQKAP--EEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDINTEAKVFYLK 129
Query: 426 MKGDYYRYLAEVATGETRHSVVEDSQKATKMLLKSARRKCSPHTP 560
M+GDY+RYL EVA G++R +E S++A K A + SP P
Sbjct: 130 MRGDYHRYLVEVAEGDSRKENIEKSREAYKDASAKA-EELSPSHP 173
>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 219
Score = 93.5 bits (222), Expect = 1e-19
Identities = 45/70 (64%), Positives = 54/70 (77%)
Frame = +1
Query: 61 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVSYKNVVGARRSS 240
M V +E L+ AKL+EQ +RYD+MA MKEV+E +LS EERNLLSVSYKN+VG RRSS
Sbjct: 80 MMVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVSYKNIVGQRRSS 139
Query: 241 WRVISQLNRK 270
WRVIS + K
Sbjct: 140 WRVISSIEEK 149
Score = 80.2 bits (189), Expect = 1e-15
Identities = 38/73 (52%), Positives = 55/73 (75%)
Frame = +3
Query: 255 SIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKG 434
SIE+KT S + K+Y+ +EKEL+++C +VLG+L++ LIP A + E+KVFY K+KG
Sbjct: 145 SIEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEENKVFYFKLKG 202
Query: 435 DYYRYLAEVATGE 473
DYYRYLAE + G+
Sbjct: 203 DYYRYLAEFSHGQ 215
>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 607
Score = 72.1 bits (169), Expect = 4e-13
Identities = 34/62 (54%), Positives = 43/62 (69%)
Frame = +1
Query: 61 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVSYKNVVGARRSS 240
M + ELVQ AKLAEQ ER++D+ MK+ E L+ E RNLLSV YKNVVG++R +
Sbjct: 186 MQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSVGYKNVVGSKRFA 245
Query: 241 WR 246
WR
Sbjct: 246 WR 247
>SB_3265| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 118
Score = 31.9 bits (69), Expect = 0.51
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 470 RNQTFCCRGFTESYQDAFEISKAKMQPTHPIR 565
+N+T CC G T SY++ F + K K P R
Sbjct: 10 KNKTICCPGRTFSYKEVFSMGKRKSASLSPQR 41
>SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 994
Score = 30.3 bits (65), Expect = 1.6
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +1
Query: 37 ISPLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEER 189
+SPLPS+ +E+V KLAE+ E ++++ T+ E + EE+
Sbjct: 643 VSPLPSTATEDQMQEVVDSNKLAEKKEVTEEVSPVKPLSTKESKENALEEK 693
>SB_32481| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 499
Score = 29.1 bits (62), Expect = 3.6
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = +2
Query: 488 CRGFTESYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNS 616
C+G TE+ Q+AFE S+ T P ++G A ++ Y+ NS
Sbjct: 212 CKGKTENLQNAFEGSE-----TDPAKIGFAFYIGLWAYDGWNS 249
>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
Length = 783
Score = 28.3 bits (60), Expect = 6.3
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +3
Query: 288 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 389
K++M ++Y K+EKE+ Y+++ L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,973,697
Number of Sequences: 59808
Number of extensions: 439994
Number of successful extensions: 1288
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1286
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1817559367
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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