Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20877
(705 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 1e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 41 7e-04
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 38 0.009
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 37 0.011
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.015
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 34 0.080
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.080
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 3.0
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 4.0
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 28 5.2
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 28 5.2
At5g11470.1 68418.m01339 bromo-adjacent homology (BAH) domain-co... 28 6.9
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 9.2
At1g34180.1 68414.m04239 no apical meristem (NAM) family protein... 27 9.2
At1g09710.1 68414.m01090 myb family transcription factor contain... 27 9.2
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 43.6 bits (98), Expect = 1e-04
Identities = 30/81 (37%), Positives = 46/81 (56%), Gaps = 8/81 (9%)
Frame = +3
Query: 282 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 437
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 438 SVESRLSSDGVLSVIAPRKVP 500
+++ + +GVLSV P KVP
Sbjct: 126 EIKASM-ENGVLSVTVP-KVP 144
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 41.1 bits (92), Expect = 7e-04
Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 282 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 437
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 438 SVESRLSSDGVLSVIAPR 491
V++ + +GVLSV P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 37.5 bits (83), Expect = 0.009
Identities = 31/115 (26%), Positives = 56/115 (48%), Gaps = 8/115 (6%)
Frame = +3
Query: 171 PAPL*AENTTARGSPCCRGSRPWLQHQKDKDKFQVNLDVQHFAPEEISVKTADGYIV-VE 347
P+ A +TAR +R + + F+ D+ EE+ V+ D ++ +
Sbjct: 33 PSSALANASTARDVAAFTNARVDWKETPEAHVFKA--DLPGLKKEEVKVEVEDKNVLQIS 90
Query: 348 GK----HEEKKDQHGYISR---QFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 491
G+ +EEK D+ + R +F RR+ LPE E V++ + +GVL+V+ P+
Sbjct: 91 GERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVPK 144
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 37.1 bits (82), Expect = 0.011
Identities = 34/127 (26%), Positives = 63/127 (49%), Gaps = 8/127 (6%)
Frame = +3
Query: 156 FSVLQPAPL*AENTTARGSPCCRGSRPWLQHQKDKDKFQVNLDVQHFAPEEISVKTAD-G 332
F +L+ PL E T+ R W ++ + ++ LD+ +E+ ++ + G
Sbjct: 50 FKILERIPLGLERDTSVALSPARVD--W---KETAEGHEIMLDIPGLKKDEVKIEVEENG 104
Query: 333 YIVVEG---KHEEKK-DQHGYISR---QFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 491
+ V G + EEKK DQ + R +F R++ LP+ ESV+++L +GVL++ +
Sbjct: 105 VLRVSGERKREEEKKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKL-ENGVLTINLTK 163
Query: 492 KVPPAVR 512
P V+
Sbjct: 164 LSPEKVK 170
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 36.7 bits (81), Expect = 0.015
Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 282 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 437
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 438 SVESRLSSDGVLSVIAPR 491
V++ + +GVL+V P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
(HSP17.7-CII) identical to heat shock protein 17.6A
GI:3256075 from [Arabidopsis thaliana]
Length = 156
Score = 34.3 bits (75), Expect = 0.080
Identities = 26/90 (28%), Positives = 44/90 (48%), Gaps = 9/90 (10%)
Frame = +3
Query: 261 DKFQVNLDVQHFAPEEISVKTA-DGYIVVEGKHE-EKKDQHGY----ISRQ---FTRRYA 413
D + +D+ +EI V+ + +VV GK + + K+ G + R+ F R++
Sbjct: 55 DAYVFAVDMPGIKGDEIQVQIENENVLVVSGKRQRDNKENEGVKFVRMERRMGKFMRKFQ 114
Query: 414 LPEGCTAESVESRLSSDGVLSVIAPRKVPP 503
LP+ E + S +DGVL V P+ PP
Sbjct: 115 LPDNADLEKI-SAACNDGVLKVTIPKLPPP 143
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 34.3 bits (75), Expect = 0.080
Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +3
Query: 282 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 437
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 438 SVESRLSSDGVLSVIAPR 491
V++ + +GVL+V P+
Sbjct: 124 QVKASM-ENGVLTVTVPK 140
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.1 bits (62), Expect = 3.0
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -1
Query: 447 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 277
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 4.0
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +3
Query: 303 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 464
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 2/70 (2%)
Frame = +3
Query: 147 RKIFSVLQPAPL*AENTTARGSPCCRGSRPWLQ--HQKDKDKFQVNLDVQHFAPEEISVK 320
R F L+P L + + R + C SRPWL H+ ++ + FA S +
Sbjct: 685 RSRFLGLKPPKLFSISVRGRSAMLCLSSRPWLGYIHRGHFHLTPLSYETLEFAAPFSSDQ 744
Query: 321 TADGYIVVEG 350
A+G + V G
Sbjct: 745 CAEGVVSVAG 754
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 2/70 (2%)
Frame = +3
Query: 147 RKIFSVLQPAPL*AENTTARGSPCCRGSRPWLQ--HQKDKDKFQVNLDVQHFAPEEISVK 320
R F L+P L + + R + C SRPWL H+ ++ + FA S +
Sbjct: 685 RSRFLGLKPPKLFSISVRGRSAMLCLSSRPWLGYIHRGHFHLTPLSYETLEFAAPFSSDQ 744
Query: 321 TADGYIVVEG 350
A+G + V G
Sbjct: 745 CAEGVVSVAG 754
>At5g11470.1 68418.m01339 bromo-adjacent homology (BAH)
domain-containing protein contains Pfam profile PF01426:
BAH domain
Length = 691
Score = 27.9 bits (59), Expect = 6.9
Identities = 17/67 (25%), Positives = 32/67 (47%)
Frame = +3
Query: 219 CRGSRPWLQHQKDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQF 398
C+ + + +K+K +Q+ + A EE S K + G H KDQ + +Q
Sbjct: 235 CKENSNGCKEEKEKGHYQLAIKKSTLA-EERSNKDSGS----RGNHYNGKDQESEVKKQL 289
Query: 399 TRRYALP 419
T++ ++P
Sbjct: 290 TKQKSMP 296
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -1
Query: 468 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 325
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At1g34180.1 68414.m04239 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to NAM-like protein GI:8809651 from (Arabidopsis
thaliana)
Length = 564
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +3
Query: 342 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 491
+E E D G RQF +P+G +AE ++S L ++ I PR
Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278
>At1g09710.1 68414.m01090 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 689
Score = 27.5 bits (58), Expect = 9.2
Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = +2
Query: 362 EERSAWLHIASIHSSLRFAGGLYG*ICGVPAVFRWCVVCNRTQEGAASSEGERKI-PIAQ 538
E+ WL +A ++ L + L+ CG+ C + G +SS+G+++ PI Q
Sbjct: 348 EDALVWLPLACLNQKLAY---LFN--CGLMPTTSSCTITETEANGGSSSQGQQQSKPIVQ 402
Query: 539 TGP 547
P
Sbjct: 403 ALP 405
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,439,618
Number of Sequences: 28952
Number of extensions: 340412
Number of successful extensions: 944
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 914
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 944
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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