Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20873
(726 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC227.02c |||rRNA processing protein Rrp15 |Schizosaccharomyce... 49 6e-07
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 34 0.018
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 33 0.031
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.39
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 29 0.68
SPBC3B9.19 |mge1||GrpE domain chaperone protein|Schizosaccharomy... 29 0.68
SPAC4H3.06 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 28 1.2
SPBC1289.02c |uap2||U2 snRNP-associated protein Uap2|Schizosacch... 27 2.1
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c... 27 2.7
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 3.6
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 3.6
SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po... 27 3.6
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 27 3.6
SPAC1705.03c ||SPAC23H4.19|conserved fungal family|Schizosacchar... 26 4.8
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 26 4.8
SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1... 26 6.3
SPAC22A12.14c |||BSD domain protein, unknown biological role|Sch... 26 6.3
SPCC1739.07 |||substrate-specific nuclear cofactor for exosome a... 26 6.3
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 26 6.3
SPCC188.06c |srp54||signal recognition particle subunit Srp54|Sc... 25 8.3
SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po... 25 8.3
SPCC1827.02c |||cholinephosphate cytidylyltransferase |Schizosac... 25 8.3
SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|c... 25 8.3
>SPAC227.02c |||rRNA processing protein Rrp15 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 205
Score = 49.2 bits (112), Expect = 6e-07
Identities = 30/74 (40%), Positives = 46/74 (62%), Gaps = 2/74 (2%)
Frame = +3
Query: 219 INKN*AKRLRKGRERLLNKIATKGVVQLFNAVRNQQKSIEKEMDRNDLSEGK--KEKILK 392
+N AK L ER L +IA +GVVQLFNAVR Q ++ ++R ++S G+ +E+ +K
Sbjct: 135 VNAETAKALFT-HERELRRIARRGVVQLFNAVRTAQ--LQSSLNRENISGGRTAREQKVK 191
Query: 393 KFDKRTFLDTLMGQ 434
+ K +FLD + Q
Sbjct: 192 ELSKASFLDLIKSQ 205
>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 814
Score = 34.3 bits (75), Expect = 0.018
Identities = 20/59 (33%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
Frame = +1
Query: 13 SKILGSSKPKN-KKTLVLSRAKKHSEIVKKVKEEKPSFEVIGEVNEIESKVKKEEINVE 186
+K++G+ KN KKT V+ + +EI K + K + G N +S VKKEE + +
Sbjct: 81 AKVMGNGGLKNGKKTAVVKEEEDFNEIAKPSPKHKRVSKANGSKNGAKSAVKKEESDTD 139
>SPAC694.06c |mrc1||mediator of replication checkpoint 1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1019
Score = 33.5 bits (73), Expect = 0.031
Identities = 23/77 (29%), Positives = 38/77 (49%), Gaps = 4/77 (5%)
Frame = +1
Query: 40 KNKKTLVLSRAKKHSEIVKKVKEEKPSFEVIG--EVNEIESK-VKKEEIN-VEPPAKKVK 207
+N L A K +VKK + F+ IG N++E+K + +EE N EPP + +
Sbjct: 216 RNTARLTRETALKPEVVVKKKVTLREFFQKIGFKNDNQLENKAISEEEANSTEPPNVEKE 275
Query: 208 HEKPSIRIKPNVLEKEE 258
KPS+ ++ E+
Sbjct: 276 EPKPSVDRSTGIVNSED 292
>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 230
Score = 29.9 bits (64), Expect = 0.39
Identities = 21/65 (32%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = -2
Query: 440 FTLSHKGIQESSLIKFFQNLFFLSFRQVIPIHFFLNRFLLISY-CIE*LHYTFSGYFVQ* 264
FT S + L+ FF LFFLSF FFL++ ++ + LH+ F +
Sbjct: 105 FTGSELSLFRCLLLFFFFLLFFLSFSFSFSFLFFLSQIFIVYFSSFPILHFLFFFFLCVC 164
Query: 263 SFSSF 249
F SF
Sbjct: 165 VFLSF 169
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 29.1 bits (62), Expect = 0.68
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +1
Query: 151 ESKVKKEEINVEPPAKKVKHEKPSIRIK 234
E ++K EE VEPP+KK+ + +PS I+
Sbjct: 1658 EKRMKNEEF-VEPPSKKLANSEPSKEIR 1684
>SPBC3B9.19 |mge1||GrpE domain chaperone protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 223
Score = 29.1 bits (62), Expect = 0.68
Identities = 22/68 (32%), Positives = 35/68 (51%), Gaps = 8/68 (11%)
Frame = +1
Query: 49 KTLVLSRAKKHSEI---VKKVKEEKPSFEVIGE-----VNEIESKVKKEEINVEPPAKKV 204
+TL+ + K S + + KEEKP+ E + E V E++SK+ + + E K+V
Sbjct: 15 RTLIRAPINKRSFLWYSTEAAKEEKPAEEKVAETENVDVKELQSKLSELKSKYEAKDKEV 74
Query: 205 KHEKPSIR 228
K SIR
Sbjct: 75 AELKGSIR 82
>SPAC4H3.06 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 28.3 bits (60), Expect = 1.2
Identities = 23/104 (22%), Positives = 50/104 (48%), Gaps = 1/104 (0%)
Frame = +3
Query: 219 INKN*AKRLRKGRERLLNKIATKGVVQLFNAVRNQQKSIEKEMDRNDLSEGKKEKILKKF 398
+N N K++ + ++ ++ T+G+ ++ + +RN Q S+ KE+ L + +EK K
Sbjct: 26 LNANQEKKIGLEQMGIIVQLVTEGLNEVSSDIRNYQASLTKELKL--LVDSLQEKERSKL 83
Query: 399 DKRTFLDTL-MGQSKSIVVDEHVKSLKDEVKDETDRPNGML*EM 527
L+ L + + S V + + L+ + + N +L M
Sbjct: 84 QATVKLEQLKVVSTNSPVENTQISELEARLSSLSKEINDILQNM 127
>SPBC1289.02c |uap2||U2 snRNP-associated protein
Uap2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 367
Score = 27.5 bits (58), Expect = 2.1
Identities = 15/65 (23%), Positives = 34/65 (52%)
Frame = +3
Query: 309 AVRNQQKSIEKEMDRNDLSEGKKEKILKKFDKRTFLDTLMGQSKSIVVDEHVKSLKDEVK 488
A+R +Q+ +++ +D+ E +K K+F+K L + + E + LKD++
Sbjct: 218 ALRLRQQQMQQISSWDDVDEEVDDKRKKRFNKIVVLKHIFTLEELDKTPELLIDLKDDIT 277
Query: 489 DETDR 503
+E ++
Sbjct: 278 EEAEK 282
>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 521
Score = 27.1 bits (57), Expect = 2.7
Identities = 18/62 (29%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +1
Query: 85 EIVKKVKEEKPSFEVIGEVNEIESKVKKEEINVEPPAKKVKHEKPSIRIKP-NVLEKEEK 261
+I+ +V PSFE + ++ EI + + IN+ ++ KP I++ P V+ E
Sbjct: 173 KIIIEVNTAIPSFEGLHDIVEIPNPPHRTPININRVDDRI--GKPYIKVDPKKVIGIVEA 230
Query: 262 DY 267
DY
Sbjct: 231 DY 232
>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 608
Score = 26.6 bits (56), Expect = 3.6
Identities = 15/26 (57%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = +1
Query: 28 SSKPKNKKTLVLS-RAKKHSEIVKKV 102
+SKPK KKTLV S K H V+KV
Sbjct: 478 TSKPKAKKTLVTSIPTKPHQASVEKV 503
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 26.6 bits (56), Expect = 3.6
Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 6/55 (10%)
Frame = +1
Query: 85 EIVKKVKEEKPSFEVIGEVNEIESKVKKEEIN---VEPPAKKVKHE---KPSIRI 231
+++ K+K +KP E+I E E ++ +IN ++ P K HE KP +R+
Sbjct: 375 DVIAKMKVQKPPKELIDEEGECTICMEMFKINDDVIQLPCKHYFHENCIKPWLRV 429
>SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 833
Score = 26.6 bits (56), Expect = 3.6
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 3/42 (7%)
Frame = +3
Query: 309 AVRNQQKSIEKEMDRNDLSEGKKEKILKKFD---KRTFLDTL 425
A +N + ++K+ +L ++EK KK D KR FLDTL
Sbjct: 98 ASKNIELKLQKKEKEEELRNLEEEKAKKKKDANLKRKFLDTL 139
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.6 bits (56), Expect = 3.6
Identities = 20/84 (23%), Positives = 41/84 (48%), Gaps = 8/84 (9%)
Frame = +1
Query: 40 KNKKTLVLSRAKKHSEIVKKVKEEKPSFEVIGEVNEI------ESKVKKEEINVEPPAKK 201
+++ +L+ KKH+ +VKK ++ E+ +V I K+ E+ NV P
Sbjct: 124 RHESVKILNIIKKHAPVVKKASIDECFIELTSDVKRIVLEEYPYLKIPSEDSNVALPQAP 183
Query: 202 VK--HEKPSIRIKPNVLEKEEKDY 267
V + + I+ V+++ ++DY
Sbjct: 184 VLLWPAEFGMVIEEEVVDRTKEDY 207
>SPAC1705.03c ||SPAC23H4.19|conserved fungal
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 421
Score = 26.2 bits (55), Expect = 4.8
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = -2
Query: 236 GLILIDGFSCFTFLAGGSTLISSFLTFD 153
GL IDGF + + GG L+ +F + D
Sbjct: 278 GLTSIDGFPVISEIGGGLVLLGNFSSID 305
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 26.2 bits (55), Expect = 4.8
Identities = 10/41 (24%), Positives = 25/41 (60%)
Frame = +1
Query: 127 VIGEVNEIESKVKKEEINVEPPAKKVKHEKPSIRIKPNVLE 249
++ ++ +++ + KK++ + KK+ H + S++ K N LE
Sbjct: 796 LVQQIRKLKDENKKDQSGTDKLMKKIYHCEQSLKEKTNSLE 836
>SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1
complex subunit Tpr1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1039
Score = 25.8 bits (54), Expect = 6.3
Identities = 21/78 (26%), Positives = 36/78 (46%)
Frame = +3
Query: 246 RKGRERLLNKIATKGVVQLFNAVRNQQKSIEKEMDRNDLSEGKKEKILKKFDKRTFLDTL 425
++ RER L + K ++ ++QQK+ E +M +D E + K KK K+ +
Sbjct: 896 KQERERQLQEERQKMQEEVLEWRKSQQKASEDDMSLSDDEEKQSGKKKKKDRKKRKSKSK 955
Query: 426 MGQSKSIVVDEHVKSLKD 479
S S V ++ L D
Sbjct: 956 QESSDSGVSEDDEIPLSD 973
>SPAC22A12.14c |||BSD domain protein, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 347
Score = 25.8 bits (54), Expect = 6.3
Identities = 21/70 (30%), Positives = 34/70 (48%), Gaps = 3/70 (4%)
Frame = +3
Query: 285 KGVVQLFNAVR--NQQKSIEKEMD-RNDLSEGKKEKILKKFDKRTFLDTLMGQSKSIVVD 455
K VVQ A++ N ++ I D R+D E E++ + + + DT S + V+D
Sbjct: 215 KEVVQPIKAIQSGNDEEEIFSWGDERSDEEESDNEQVNDEKKQSSEEDTTENNSAAEVID 274
Query: 456 EHVKSLKDEV 485
E V L+ V
Sbjct: 275 ETVNDLESAV 284
>SPCC1739.07 |||substrate-specific nuclear cofactor for exosome
activity |Schizosaccharomyces pombe|chr 3|||Manual
Length = 133
Score = 25.8 bits (54), Expect = 6.3
Identities = 15/56 (26%), Positives = 25/56 (44%)
Frame = +1
Query: 109 EKPSFEVIGEVNEIESKVKKEEINVEPPAKKVKHEKPSIRIKPNVLEKEEKDY*TK 276
E+P + + V SK+++ E NV P + V +I + K KD T+
Sbjct: 72 ERPVMQELQRVKNYISKIQQAEKNVNPKTEAVNTSNAAISSSSSNRPKVAKDAATR 127
>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 2410
Score = 25.8 bits (54), Expect = 6.3
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 325 KNLLRKKWIGMTCLKERKKRF*KNLIRELSWIPLWD 432
K+LLR+K + C+ + F + + SW LWD
Sbjct: 2190 KSLLRRKLVVWFCISVFLQNFWLSSLNGRSWSYLWD 2225
>SPCC188.06c |srp54||signal recognition particle subunit
Srp54|Schizosaccharomyces pombe|chr 3|||Manual
Length = 522
Score = 25.4 bits (53), Expect = 8.3
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = +3
Query: 360 LSEGKKEKILKKFDKRTFLDTLMGQSKSIVVDEHVKSLKDEVKD 491
+ G+ L++F R+F+ L+G + EHV+SL + K+
Sbjct: 273 IGTGEHINDLERFSPRSFISKLLGLGDLEGLMEHVQSLDFDKKN 316
>SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 25.4 bits (53), Expect = 8.3
Identities = 17/56 (30%), Positives = 24/56 (42%)
Frame = +1
Query: 91 VKKVKEEKPSFEVIGEVNEIESKVKKEEINVEPPAKKVKHEKPSIRIKPNVLEKEE 258
V V++ K V + + KKEE + AKK K EK + K +K E
Sbjct: 419 VNGVEDSKKRKSVESSEKDEDEAAKKEEKRRKKEAKKEKKEKKEKKEKKEKKKKSE 474
>SPCC1827.02c |||cholinephosphate cytidylyltransferase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 354
Score = 25.4 bits (53), Expect = 8.3
Identities = 21/65 (32%), Positives = 36/65 (55%)
Frame = +1
Query: 70 AKKHSEIVKKVKEEKPSFEVIGEVNEIESKVKKEEINVEPPAKKVKHEKPSIRIKPNVLE 249
++K + ++ K K +FE I E E E K +KE+ E P+K + E+ S + P +E
Sbjct: 14 SEKEDNVERQTK--KYNFE-IDEPEEQEKKDEKEDDKEESPSKSL--EEISQSVSP--VE 66
Query: 250 KEEKD 264
+E +D
Sbjct: 67 EEPRD 71
>SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 240
Score = 25.4 bits (53), Expect = 8.3
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +3
Query: 390 KKFDKRTFLDTLMGQSKSIVVDEHVKSLKDEVK 488
K ++K + L L+ +S H+KSLKD V+
Sbjct: 58 KLYNKASILQMLLDRSSVPKSPSHIKSLKDVVQ 90
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,648,214
Number of Sequences: 5004
Number of extensions: 52336
Number of successful extensions: 232
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 222
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -