BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20853 (724 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 31 0.22 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 28 1.2 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 28 1.2 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 27 2.7 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 27 3.6 SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2 |... 26 4.7 SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces po... 25 8.3 SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 25 8.3 SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 25 8.3 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 30.7 bits (66), Expect = 0.22 Identities = 20/80 (25%), Positives = 39/80 (48%), Gaps = 5/80 (6%) Frame = +2 Query: 29 RSIQELMAESGRQSAEGDASLGLQMSFDTTQKTEDSDMKLDFGFDSD-----LGQLSEDK 193 ++I E +A+ +++ GD ++ + M FD + ++S+ + D+D + QLS Sbjct: 81 KTISEFLAQKIWKTSNGDLNVAVDMYFDESFNIKNSNPDSESQKDTDASLTQMDQLSNTV 140 Query: 194 SAKSLG*PRGVHMSTSNTIS 253 S K L R + N +S Sbjct: 141 SVKDLSINRNTNKKALNAVS 160 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 28.3 bits (60), Expect = 1.2 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = -2 Query: 618 EYQENHQIDLYSDPRKLRKVHPAGEAVEKILT 523 E++ +ID+ DPR LR++ A E ++ L+ Sbjct: 246 EFKRKQKIDISDDPRALRRLRSACERAKRALS 277 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 28.3 bits (60), Expect = 1.2 Identities = 11/19 (57%), Positives = 14/19 (73%) Frame = -2 Query: 543 AVEKILTIAVMSKQHGRRR 487 A+E+ LTI +M QHG RR Sbjct: 341 AIERALTIRIMQTQHGARR 359 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 27.1 bits (57), Expect = 2.7 Identities = 12/41 (29%), Positives = 24/41 (58%) Frame = +2 Query: 251 SLWQLILI*KSLVLRRSGRCLLLRKIRRNYNLLDLKKPIQK 373 S+W ++ S+ + SG L + KIR+ ++ L + P+Q+ Sbjct: 1372 SIWNVV----SITINNSGEDLFISKIRKGHSPLIFRLPLQR 1408 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 26.6 bits (56), Expect = 3.6 Identities = 10/32 (31%), Positives = 23/32 (71%) Frame = -2 Query: 618 EYQENHQIDLYSDPRKLRKVHPAGEAVEKILT 523 E++E ++ID+ S+P+ ++ A E ++K+L+ Sbjct: 250 EFKEKYKIDVLSNPKATFRLATAVERLKKVLS 281 >SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 652 Score = 26.2 bits (55), Expect = 4.7 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = +1 Query: 457 QGGYGGLSVPSPPAMLFTHH 516 +GGYGG P P + FT H Sbjct: 120 KGGYGGFYDPRPGCLKFTTH 139 >SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 25.4 bits (53), Expect = 8.3 Identities = 18/48 (37%), Positives = 26/48 (54%), Gaps = 3/48 (6%) Frame = -2 Query: 642 GPLNRRHMEY-QENHQIDLYSDP--RKLRKVHPAGEAVEKILTIAVMS 508 G L R+ +Y +E+H+ DL KLR + G+A E + TI MS Sbjct: 341 GDLLARYWKYFEESHKFDLNLQVYHEKLRNLVQQGQAAECLNTIKRMS 388 >SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 25.4 bits (53), Expect = 8.3 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +2 Query: 422 PHRLNITTTWAIREGTVGYQFRRRLPCCLLITAIVRIFSTASP 550 P R+NI A E + RRRL ++ I +FST P Sbjct: 257 PTRVNIADISATSEDPLSRATRRRLRLLGIMEGIPVVFSTEKP 299 >SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces pombe|chr 3|||Manual Length = 379 Score = 25.4 bits (53), Expect = 8.3 Identities = 14/39 (35%), Positives = 19/39 (48%), Gaps = 3/39 (7%) Frame = +3 Query: 585 SRGQFGGFPG---TPYGAGSAAPYNYQPPPDMFSSIPSQ 692 +R + GG PG +G G A P+ + P S PSQ Sbjct: 161 TRTRGGGMPGGFANMFGGGGAGPHARRSHPSFGGSRPSQ 199 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,021,264 Number of Sequences: 5004 Number of extensions: 60566 Number of successful extensions: 177 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 169 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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