Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20838
(662 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g61390.2 68416.m06872 U-box domain-containing protein several... 33 0.17
At2g30780.1 68415.m03753 pentatricopeptide (PPR) repeat-containi... 31 0.52
At1g51070.1 68414.m05741 basic helix-loop-helix (bHLH) family pr... 31 0.52
At5g65910.1 68418.m08296 BSD domain-containing protein contains ... 31 0.68
At1g66170.1 68414.m07511 PHD finger family protein (MMD1) contai... 31 0.68
At3g19860.1 68416.m02515 basic helix-loop-helix (bHLH) family pr... 30 1.2
At5g60800.1 68418.m07628 heavy-metal-associated domain-containin... 29 2.8
At1g03830.1 68414.m00364 guanylate-binding family protein contai... 29 2.8
At5g29624.1 68418.m03640 DC1 domain-containing protein contains ... 29 3.6
At4g36060.2 68417.m05134 basic helix-loop-helix (bHLH) family pr... 29 3.6
At4g36060.1 68417.m05133 basic helix-loop-helix (bHLH) family pr... 29 3.6
At2g30410.1 68415.m03704 tubulin folding cofactor A (KIESEL) ide... 29 3.6
At1g12380.1 68414.m01431 expressed protein 29 3.6
At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial, p... 28 6.4
At1g11670.1 68414.m01340 MATE efflux family protein similar to r... 28 6.4
At5g54050.1 68418.m06722 DC1 domain-containing protein 27 8.4
At4g03430.1 68417.m00470 pre-mRNA splicing factor-related simila... 27 8.4
At3g15920.1 68416.m02013 phox (PX) domain-containing protein wea... 27 8.4
>At3g61390.2 68416.m06872 U-box domain-containing protein several
hypothetical proteins - Arabidopsis thaliana
Length = 435
Score = 33.1 bits (72), Expect = 0.17
Identities = 27/124 (21%), Positives = 52/124 (41%), Gaps = 2/124 (1%)
Frame = +3
Query: 243 E*FRNVEVPDENEEIKRPLVDLRN-PGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAE 419
E FR + +E EE+K L ++ G + +E T N H E + + + +DI
Sbjct: 314 ETFRGIR--EEQEELKIKLREVSKLKGKREEEEASTSN--HREPPQYFICPITHDIMEDP 369
Query: 420 IALRQGFQEVSDGIGKWYARTEQINE-LQASCNISKKISALRYKS*MKRYILLNQLTPSR 596
GF + I +W+ R + + + + + L +S ++ ++ L +L
Sbjct: 370 HVAADGFTYEGEAISRWFERGHETSPMINKRLPHTSLVPNLALRSAIQEWLQLRELLNRP 429
Query: 597 PFCR 608
CR
Sbjct: 430 SACR 433
>At2g30780.1 68415.m03753 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 452
Score = 31.5 bits (68), Expect = 0.52
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = +3
Query: 345 TQNPEHHEDAEKIVSSVKNDINTAEIALRQGFQEVSDGIGKWYART 482
T P HH D + VS +K+++ T + ++ FQ V D G+W RT
Sbjct: 20 TSLPAHHTDLVQRVSILKDELLTIGNS-KEKFQNVLDQKGQWLFRT 64
>At1g51070.1 68414.m05741 basic helix-loop-helix (bHLH) family
protein similar to bHLH transcription factor GI:3757520
from [Arabidopsis thaliana]
Length = 226
Score = 31.5 bits (68), Expect = 0.52
Identities = 23/96 (23%), Positives = 48/96 (50%)
Frame = +1
Query: 58 PDKVPEAEDKPLNVVDNLSSEQELIDQANTIKDIDNSLRANKKEVIDIPVKVIVEEIKPS 237
P + P+ DK + D + + D+A +KD+++SL+ KE+ D K + + K
Sbjct: 95 PGRTPKT-DKVAIINDAIRMVNQARDEAQKLKDLNSSLQEKIKELKD--EKNELRDEKQK 151
Query: 238 LKSDLETLKCRMKMRKSRGL*SI*EIPGPRSIKSTK 345
LK + E + ++K K++ +P P+++ +
Sbjct: 152 LKVEKERIDQQLKAIKTQPQPQPCFLPNPQTLSQAQ 187
>At5g65910.1 68418.m08296 BSD domain-containing protein contains
Pfam profile PF03909: BSD domain
Length = 432
Score = 31.1 bits (67), Expect = 0.68
Identities = 13/52 (25%), Positives = 23/52 (44%)
Frame = +3
Query: 306 LRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALRQGFQEVSDGI 461
L P + Q P+H E+ E +++ +KND R G ++S +
Sbjct: 64 LSQPSSSPDLQERNQTPDHPEEDEDLIAGIKNDFAEIGGRFRTGISKLSGNL 115
>At1g66170.1 68414.m07511 PHD finger family protein (MMD1) contains
Pfam profile: PF00628: PHD-finger
Length = 704
Score = 31.1 bits (67), Expect = 0.68
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -2
Query: 502 WSSLICSVRAYHFPIPSDTSW 440
WS+ S R YHF IP DT W
Sbjct: 100 WSNHFVSKRKYHFIIPIDTEW 120
>At3g19860.1 68416.m02515 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 284
Score = 30.3 bits (65), Expect = 1.2
Identities = 20/67 (29%), Positives = 32/67 (47%)
Frame = +1
Query: 82 DKPLNVVDNLSSEQELIDQANTIKDIDNSLRANKKEVIDIPVKVIVEEIKPSLKSDLETL 261
DK + D + +EL + N +K +L +E+ K + E K SLKSD+E L
Sbjct: 40 DKATILTDTVQLLKELTSEVNKLKSEYTALTDESRELTQ--EKNDLREEKTSLKSDIENL 97
Query: 262 KCRMKMR 282
+ + R
Sbjct: 98 NLQYQQR 104
>At5g60800.1 68418.m07628 heavy-metal-associated domain-containing
protein similar to farnesylated protein ATFP3
[GI:4097547]; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 283
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/51 (33%), Positives = 29/51 (56%)
Frame = +3
Query: 258 VEVPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDIN 410
V++ ++ EE + VDL +P P + +E E +N ++ ED +K K D N
Sbjct: 74 VKLREKLEEKTKKKVDLVSPQPKKEKEKENKN-KNDEDKKKSEEKKKPDNN 123
>At1g03830.1 68414.m00364 guanylate-binding family protein contains
Pfam domains PF02263: Guanylate-binding protein,
N-terminal domain and PF02841: Guanylate-binding
protein, C-terminal domain
Length = 991
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/64 (26%), Positives = 33/64 (51%)
Frame = +1
Query: 97 VVDNLSSEQELIDQANTIKDIDNSLRANKKEVIDIPVKVIVEEIKPSLKSDLETLKCRMK 276
V + +++ +E + QA+T +D LRA V+D K+I E+ + + R +
Sbjct: 536 VTEKIAAMEEKLKQASTTED---GLRAEFSRVLDEKEKIITEKAAKLATLEQQLASTRAE 592
Query: 277 MRKS 288
++KS
Sbjct: 593 LKKS 596
>At5g29624.1 68418.m03640 DC1 domain-containing protein contains
Pfam PF03107: DC1 domain
Length = 488
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +3
Query: 261 EVPDENEEIKRPLVDLRNPGPPQHQEHE 344
+VPDE EEIK P + G H HE
Sbjct: 264 DVPDEEEEIKDPFKVINENGDIVHISHE 291
>At4g36060.2 68417.m05134 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 268
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/67 (26%), Positives = 35/67 (52%)
Frame = +1
Query: 82 DKPLNVVDNLSSEQELIDQANTIKDIDNSLRANKKEVIDIPVKVIVEEIKPSLKSDLETL 261
DK + D + +++++Q + +K +L +E+I K + E K +LKSD+E L
Sbjct: 61 DKASVLTDTIQMLKDVMNQVDRLKAEYETLSQESRELIQ--EKSELREEKATLKSDIEIL 118
Query: 262 KCRMKMR 282
+ + R
Sbjct: 119 NAQYQHR 125
>At4g36060.1 68417.m05133 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 286
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/67 (26%), Positives = 35/67 (52%)
Frame = +1
Query: 82 DKPLNVVDNLSSEQELIDQANTIKDIDNSLRANKKEVIDIPVKVIVEEIKPSLKSDLETL 261
DK + D + +++++Q + +K +L +E+I K + E K +LKSD+E L
Sbjct: 79 DKASVLTDTIQMLKDVMNQVDRLKAEYETLSQESRELIQ--EKSELREEKATLKSDIEIL 136
Query: 262 KCRMKMR 282
+ + R
Sbjct: 137 NAQYQHR 143
>At2g30410.1 68415.m03704 tubulin folding cofactor A (KIESEL)
identical to cDNA tubulin folding cofactor A,
GI:20514256, SP|O04350 Tubulin-specific chaperone A
(Tubulin-folding cofactor A) (CFA) (TCP1-chaperonin
cofactor A homolog) {Arabidopsis thaliana}
Length = 113
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +3
Query: 264 VPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAE 419
+PD ++ ++ L DL++ + E + PE EDA+K V+ V+ T +
Sbjct: 62 IPDCHKRLESALADLKSTLAELEETDEKEGPE-IEDAKKTVADVEKQFPTED 112
>At1g12380.1 68414.m01431 expressed protein
Length = 793
Score = 28.7 bits (61), Expect = 3.6
Identities = 17/68 (25%), Positives = 35/68 (51%)
Frame = +3
Query: 294 PLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALRQGFQEVSDGIGKWY 473
PL +R+ + +PE +D +K+++ + + + A IAL + + ++G+ Y
Sbjct: 609 PLYLIRDSSGKYLPPFKCLSPEQEKDVDKLITRLVSR-DEAHIALMELMKWRTEGLDPMY 667
Query: 474 ARTEQINE 497
AR Q+ E
Sbjct: 668 ARAVQMKE 675
>At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial,
putative / HSP70, mitochondrial, putative strong
similarity to SP|Q01899 Heat shock 70 kDa protein,
mitochondrial precursor {Phaseolus vulgaris}
Length = 682
Score = 27.9 bits (59), Expect = 6.4
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 2/85 (2%)
Frame = +3
Query: 252 RNVEVPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALR 431
+ E+ + ++ K+ L+DLRN E E+ EKI + + ++I TA LR
Sbjct: 561 KEAELNAQKDQEKKQLIDLRNSADTTIYSVEKSLSEYR---EKIPAEIASEIETAVSDLR 617
Query: 432 QGF--QEVSDGIGKWYARTEQINEL 500
++V D K A + ++++
Sbjct: 618 TAMAGEDVEDIKAKVEAANKAVSKI 642
>At1g11670.1 68414.m01340 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
Uncharacterized membrane protein family; EST gb|W43487
comes from this gene
Length = 503
Score = 27.9 bits (59), Expect = 6.4
Identities = 20/56 (35%), Positives = 26/56 (46%)
Frame = -1
Query: 203 GMSMTSFLFARRLLSMSLMVLAWSMSSCSLDKLSTTFKGLSSASGTLSGMEXRVRR 36
GMSM + +FA RL SM L + S ++ L G+ SA TL G R
Sbjct: 70 GMSMLTRIFAGRLGSMQLAAASLGNSGFNMFTLGLML-GMGSAVETLCGQAHGAHR 124
>At5g54050.1 68418.m06722 DC1 domain-containing protein
Length = 580
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 261 EVPDENEEIKRPLVDLRNPGPPQHQEHE 344
+VP+E EEIK P + G H HE
Sbjct: 276 DVPEEEEEIKDPFKVINEKGDIVHFSHE 303
>At4g03430.1 68417.m00470 pre-mRNA splicing factor-related similar
to pre-mRNA splicing factor pre-mRNA splicing factor
prp1 (SP:Q12381) [Fission yeast]
Length = 1029
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/40 (27%), Positives = 20/40 (50%)
Frame = +3
Query: 387 SSVKNDINTAEIALRQGFQEVSDGIGKWYARTEQINELQA 506
+ +++D+ LR+G + + D + W A E NE A
Sbjct: 458 AKLEHDVENKSRVLRKGLEHIPDSVRLWKAVVELANEEDA 497
>At3g15920.1 68416.m02013 phox (PX) domain-containing protein weak
similarity to myosin heavy chain [Rana catesbeiana]
GI:4249699; contains Pfam profile PF00787: PX domain
Length = 755
Score = 27.5 bits (58), Expect = 8.4
Identities = 16/60 (26%), Positives = 30/60 (50%)
Frame = +3
Query: 255 NVEVPDENEEIKRPLVDLRNPGPPQHQEHETQNPEHHEDAEKIVSSVKNDINTAEIALRQ 434
N + EN+ + + + D+R H+EHE + + + +V VK+ + T + LRQ
Sbjct: 526 NESLVQENQMLLQQINDIRENFENFHKEHEELEVKAKAELKVLVKEVKS-LRTTQSDLRQ 584
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,444,162
Number of Sequences: 28952
Number of extensions: 237574
Number of successful extensions: 968
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 927
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 967
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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