Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20828
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccha... 144 1e-35
SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces p... 144 1e-35
SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schiz... 38 0.002
SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual 30 0.25
SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ... 29 0.59
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.59
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 27 2.4
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 26 5.5
SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 26 5.5
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 26 5.5
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 9.5
>SPAC328.10c |rps502|rps5-2|40S ribosomal protein
S5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 203
Score = 144 bits (349), Expect = 1e-35
Identities = 71/89 (79%), Positives = 77/89 (86%)
Frame = +3
Query: 243 TQAFRNTQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAII 422
T+ FR +C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVLV A+
Sbjct: 57 TKRFRKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVA 116
Query: 423 NSGPREDSTRIGRAGTVRRQAVDVSPLRR 509
GPREDSTRIG AGTVRRQAVDVSPLRR
Sbjct: 117 ACGPREDSTRIGSAGTVRRQAVDVSPLRR 145
Score = 74.5 bits (175), Expect = 1e-14
Identities = 34/50 (68%), Positives = 44/50 (88%)
Frame = +2
Query: 500 LAPVNQAIWLLCTGAREAAFRNIKTIAECVADELINAAKGSSNSYASKRR 649
L VNQA+ L+ GAREAAFRN+K+I+EC+A+E+INAAKGSSNSYA K++
Sbjct: 143 LRRVNQALALITIGAREAAFRNVKSISECLAEEIINAAKGSSNSYAIKKK 192
Score = 44.8 bits (101), Expect = 1e-05
Identities = 25/64 (39%), Positives = 39/64 (60%)
Frame = +1
Query: 64 AGSVVVETMSLPQAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 243
A S++ + +SL + IKLF ++ V+V D+SL DYI++ + LPH+AGR+
Sbjct: 2 AASIIPKEVSLDETG---HIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQ 56
Query: 244 HKRF 255
KRF
Sbjct: 57 TKRF 60
>SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 144 bits (349), Expect = 1e-35
Identities = 71/89 (79%), Positives = 77/89 (86%)
Frame = +3
Query: 243 TQAFRNTQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAII 422
T+ FR +C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVLV A+
Sbjct: 57 TKRFRKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVA 116
Query: 423 NSGPREDSTRIGRAGTVRRQAVDVSPLRR 509
GPREDSTRIG AGTVRRQAVDVSPLRR
Sbjct: 117 ACGPREDSTRIGSAGTVRRQAVDVSPLRR 145
Score = 74.5 bits (175), Expect = 1e-14
Identities = 34/50 (68%), Positives = 44/50 (88%)
Frame = +2
Query: 500 LAPVNQAIWLLCTGAREAAFRNIKTIAECVADELINAAKGSSNSYASKRR 649
L VNQA+ L+ GAREAAFRN+K+I+EC+A+E+INAAKGSSNSYA K++
Sbjct: 143 LRRVNQALALITIGAREAAFRNVKSISECLAEEIINAAKGSSNSYAIKKK 192
Score = 42.3 bits (95), Expect = 6e-05
Identities = 25/60 (41%), Positives = 35/60 (58%), Gaps = 3/60 (5%)
Frame = +1
Query: 85 TMSLPQAADIPE---IKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYAHKRF 255
T SL + E IKLF ++ V+V D+SL DYI++ + LPH+AGR+ KRF
Sbjct: 3 TSSLTPGVSLDENGSIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQTKRF 60
>SPAC16E8.10c |||mitochondrial ribosomal protein subunit
S7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 259
Score = 37.5 bits (83), Expect = 0.002
Identities = 23/53 (43%), Positives = 28/53 (52%)
Frame = +3
Query: 276 VERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGP 434
V+ L N +M +GKK A +IV A II TGENP+ VL AI P
Sbjct: 126 VQHLVNLIM----RDGKKAKAEKIVATALSIIQKETGENPIDVLKQAIAEISP 174
>SPCC736.08 |cbf11||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual
Length = 613
Score = 30.3 bits (65), Expect = 0.25
Identities = 20/66 (30%), Positives = 35/66 (53%), Gaps = 1/66 (1%)
Frame = -2
Query: 295 EFVRRSTIGHW-VLRNACVHTCQLNEVNILRTSL*RKCSPAETYQRLAHRSNSICRKA*F 119
E +RR+ H +L N C+ LNE ++ + SL R P++ ++ R +S+ +K+
Sbjct: 135 EMIRRAISDHLDLLNNCCIGIANLNEDSVHKISLTRSGKPSQLV-TVSCRHSSVIQKSYG 193
Query: 118 QECRRL 101
E R L
Sbjct: 194 SEKRYL 199
>SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 335
Score = 29.1 bits (62), Expect = 0.59
Identities = 18/52 (34%), Positives = 29/52 (55%)
Frame = +3
Query: 357 AFEIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRS 512
+ +I HL+TG N + T ++N R D R+ R+ + Q+ D SP +RS
Sbjct: 159 SLDIHHLVTGHNADDIAETILMNL-LRGDVARLPRSTEITTQS-DSSPTKRS 208
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 29.1 bits (62), Expect = 0.59
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +2
Query: 335 GRTYCQTCV*NYSLVNWRKPSASTRDCHYQLWT 433
G TYC C L+NW K S S C +L+T
Sbjct: 101 GHTYCYEC-----LLNWLKESKSCPTCRQKLYT 128
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 27.1 bits (57), Expect = 2.4
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +1
Query: 100 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 243
+ ADI + FGR ++++++ + I+V+EKYAK P R A
Sbjct: 7 KVADI-SLAAFGR---KELEIAENEMPGLIAVREKYAKSQPLKGARIA 50
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = +2
Query: 284 PYKLSNDARSEQWQKTDGRTYCQTCV*NYSLVNWRKPSAST 406
PYK+ + + W + G+TY + + + L R+ +AST
Sbjct: 138 PYKIVEHSNGDAWLEARGKTYSPSQIGGFILSKMRE-TAST 177
>SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 743
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +2
Query: 197 KRSTQNIYLIQLAGMHTSVS*HPVPNRGAPYKLSN 301
K+S N+ IQ +HT + HP N P+ +N
Sbjct: 173 KKSRLNLQEIQKNSLHTGNTSHPYANASFPHDPAN 207
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 25.8 bits (54), Expect = 5.5
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = +1
Query: 214 YLPHSAGRYAHKRFVTPSAQSWSALQTL**CTVGTMAKN*WP 339
Y+ SAGR F + WS++ TL T + K WP
Sbjct: 1121 YVTQSAGRIMRAIFEISLRRGWSSVATLSLDTCKMIEKRLWP 1162
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +2
Query: 89 CLYHKPPTFLKSSFS 133
C H P TFL SSFS
Sbjct: 117 CFIHPPITFLSSSFS 131
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,701,430
Number of Sequences: 5004
Number of extensions: 55851
Number of successful extensions: 171
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -