Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20825
(709 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATP... 68 5e-12
At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putati... 64 7e-11
At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putati... 55 4e-08
At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id... 31 0.99
At1g63730.1 68414.m07212 disease resistance protein (TIR-NBS-LRR... 30 1.7
At5g26680.1 68418.m03171 endonuclease, putative similar to Swiss... 29 2.3
At5g60350.1 68418.m07566 hypothetical protein 29 3.0
At5g05400.1 68418.m00582 disease resistance protein (CC-NBS-LRR ... 28 7.0
At3g46490.1 68416.m05047 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 7.0
At2g02180.1 68415.m00154 tobamovirus multiplication protein 3 (T... 28 7.0
At5g55600.1 68418.m06932 agenet domain-containing protein / brom... 27 9.2
At3g58810.2 68416.m06555 zinc transporter, putative similar to z... 27 9.2
At3g58810.1 68416.m06554 zinc transporter, putative similar to z... 27 9.2
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 27 9.2
>At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATPase
E subunit / vacuolar proton pump E subunit (VATE)
identical to SP|Q39258 Vacuolar ATP synthase subunit E
(EC 3.6.3.14) (V-ATPase E subunit) (Vacuolar proton pump
E subunit) {Arabidopsis thaliana}
Length = 230
Score = 68.1 bits (159), Expect = 5e-12
Identities = 39/96 (40%), Positives = 58/96 (60%)
Frame = +3
Query: 219 KEGEAG*TSEEDQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSELLVT 398
KE +A + D S LN +R+KVL+ ++D V + D+A K L V +D Y +LL
Sbjct: 58 KEKQADVRKKIDYSMQ-LNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYAYKQLLKD 116
Query: 399 LIVQALFQLMEPTVTIRVRQTDKALVESLLGKAQTD 506
LIVQ L +L EP+V +R R+ D LVE++L A+ +
Sbjct: 117 LIVQCLLRLKEPSVLLRCREEDLGLVEAVLDDAKEE 152
Score = 38.7 bits (86), Expect = 0.004
Identities = 17/40 (42%), Positives = 27/40 (67%)
Frame = +2
Query: 578 GGIELVAARGRIKISNTLESRLELIAQQLLPEIRNALFGR 697
GG+ L + G+I NTL++RL++ + LP IR +LFG+
Sbjct: 188 GGVVLASRDGKIVCENTLDARLDVAFRMKLPVIRKSLFGQ 227
>At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 235
Score = 64.5 bits (150), Expect = 7e-11
Identities = 32/80 (40%), Positives = 51/80 (63%)
Frame = +3
Query: 261 SNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSELLVTLIVQALFQLMEPTV 440
S LN +R+K L+ ++D V + D A K L V D Y +LL +LI+++L +L EP+V
Sbjct: 71 STQLNASRIKYLQAQDDVVTAMKDSAAKDLLRVSNDKNNYKKLLKSLIIESLLRLKEPSV 130
Query: 441 TIRVRQTDKALVESLLGKAQ 500
+R R+ DK +VES++ A+
Sbjct: 131 LLRCREMDKKVVESVIEDAK 150
Score = 39.9 bits (89), Expect = 0.002
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = +2
Query: 566 PDTCGGIELVAARGRIKISNTLESRLELIAQQLLPEIRNALFG 694
P GG+ L + G+I NTL++RL++ +Q LP+IR L G
Sbjct: 186 PHCSGGVVLASQDGKIVCENTLDARLDVAFRQKLPQIRTRLVG 228
Score = 34.3 bits (75), Expect = 0.080
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +1
Query: 52 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKI 204
++DADV KQI+ M+ FI Q FNIE+ +L++ + K+
Sbjct: 1 MNDADVSKQIQQMVRFIRQEAEEKANEISISAEEEFNIERLQLLESAKRKL 51
>At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 237
Score = 55.2 bits (127), Expect = 4e-08
Identities = 34/88 (38%), Positives = 50/88 (56%), Gaps = 6/88 (6%)
Frame = +3
Query: 261 SNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKL------YSELLVTLIVQALFQ 422
S LN +R+KVL+ ++D V + +EA K+L +V + Y LL LIVQ L +
Sbjct: 71 SMQLNASRIKVLQAQDDIVNAMKEEAAKQLLKVSQHGFFNHHHHQYKHLLKDLIVQCLLR 130
Query: 423 LMEPTVTIRVRQTDKALVESLLGKAQTD 506
L EP V +R R+ D +VES+L A +
Sbjct: 131 LKEPAVLLRCREEDLDIVESMLDDASEE 158
Score = 43.2 bits (97), Expect = 2e-04
Identities = 19/40 (47%), Positives = 28/40 (70%)
Frame = +2
Query: 578 GGIELVAARGRIKISNTLESRLELIAQQLLPEIRNALFGR 697
GG+ L + G+I NTL++RLE+ + LPEIR +LFG+
Sbjct: 194 GGVVLASRDGKIVCENTLDARLEVAFRNKLPEIRKSLFGK 233
>At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly
identical to auxin transport protein; BIG [Arabidopsis
thaliana] GI:21779966; contains Pfam profiles PF02207:
Putative zinc finger in N-recognin, PF00569: Zinc finger
ZZ type
Length = 5098
Score = 30.7 bits (66), Expect = 0.99
Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = +2
Query: 362 KGHQTVLRAAGHTYC-AGSLPAHGTHCHHPRPSNRQGSGGVPARKSPN 502
+GH+ V + +C G+ G+ C +P G+G PAR + N
Sbjct: 1613 RGHRVVYSRSSRFFCDCGAGGVRGSSCQCLKPRKYNGNGSAPARGTNN 1660
>At1g63730.1 68414.m07212 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 966
Score = 29.9 bits (64), Expect = 1.7
Identities = 18/60 (30%), Positives = 34/60 (56%)
Frame = -1
Query: 616 LDTSPGCNQLDSTTGVGRQKVLGVDFQHNILLDLIL*SVWAFPSRDSTRALSV*RTRMVT 437
+D+ C+ L++ +G + V+G+ F + LL+ + S AF + R LS+ +TR+ T
Sbjct: 509 IDSDEICDVLENDSG--NRNVMGISFDISTLLNDVYISAEAFKRIRNLRFLSIYKTRLDT 566
>At5g26680.1 68418.m03171 endonuclease, putative similar to
Swiss-Prot:P39748 FLAP endonuclease-1 (Maturation factor
1) (MF1) [Homo sapiens]
Length = 453
Score = 29.5 bits (63), Expect = 2.3
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -1
Query: 652 DQLQARLQSVADLDTSPGCNQLDSTTGVGRQKVLGVDFQH 533
++LQ + DL GC+ DS G+G Q L + QH
Sbjct: 215 EELQLTMDQFIDLCILSGCDYCDSIRGIGGQTALKLIRQH 254
>At5g60350.1 68418.m07566 hypothetical protein
Length = 292
Score = 29.1 bits (62), Expect = 3.0
Identities = 20/68 (29%), Positives = 39/68 (57%)
Frame = +3
Query: 219 KEGEAG*TSEEDQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSELLVT 398
++GE G ++ E S++LN + VL+ ED + +E + +AE+ D + SE L+T
Sbjct: 66 EDGEGGKSTVEYDGSSILNTSG--VLRGDED----ISEEEPEEVAEILIDKEEDSEFLIT 119
Query: 399 LIVQALFQ 422
+++ L +
Sbjct: 120 MVLDLLVE 127
>At5g05400.1 68418.m00582 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 874
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +3
Query: 285 LKVLKVREDHVRNVLDEARKRLAEV 359
LK +KV+ED N+LDE ++ L+EV
Sbjct: 54 LKRIKVQEDRGLNLLDEVQQWLSEV 78
>At3g46490.1 68416.m05047 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to hyoscyamine 6 beta-hydroxylase
from Atropa belladonna [GI:4996123] and Hyoscyamus niger
[SP|P24397], gibberellin 20-oxidase [GI:9791186];
contains PF03171 2OG-Fe(II) oxygenase superfamily domain
Length = 306
Score = 27.9 bits (59), Expect = 7.0
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = -3
Query: 512 SLVCLGFSEQGLHQSLVGLTDADGDSGF 429
SL C+ LHQS V L A DSGF
Sbjct: 16 SLTCIDLDNSDLHQSAVLLKQACLDSGF 43
>At2g02180.1 68415.m00154 tobamovirus multiplication protein 3
(TOM3) identical to tobamovirus multiplication protein
(TOM3) GI:15425641 from [Arabidopsis thaliana]
Length = 303
Score = 27.9 bits (59), Expect = 7.0
Identities = 9/34 (26%), Positives = 21/34 (61%)
Frame = -3
Query: 365 LWHFSQALASFVKYITYVIFTNFQYLQTSLVQHV 264
++HF + + V+ + +V N Q++Q ++QH+
Sbjct: 85 VFHFLNFVVNGVRAVVFVFRRNVQFMQPEILQHI 118
>At5g55600.1 68418.m06932 agenet domain-containing protein /
bromo-adjacent homology (BAH) domain-containing protein
contains Pfam profile PF01426: BAH domain and PF05641:
Agenet domain
Length = 663
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +2
Query: 509 KNKIKKDVVLKVDTENFLSPDTCGGIELVAARGRIKISNTLESRLE 646
+NK KDVV+K D EN LS + + + + NT+E E
Sbjct: 589 QNKEHKDVVVKEDDENKLSKEEDKEVGSNETKTYVNHENTVEDHKE 634
>At3g58810.2 68416.m06555 zinc transporter, putative similar to zinc
transporter 4; ZnT4 [Mus musculus]
gi|2582990|gb|AAB82593; similar to zinc transporter ZAT
[Arabidopsis thaliana] gi|4206640|gb|AAD11757; member of
the cation diffusion facilitator (CDF) family, or cation
efflux (CE) family, PMID:11500563
Length = 393
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -2
Query: 366 PLALQPGACELRQVHYVRDLHELSVPSDELGSAC 265
P L+ G CE+ +V V +LH ++ +L AC
Sbjct: 323 PTMLEKGVCEIEEVVAVHELHIWAITVGKLLLAC 356
>At3g58810.1 68416.m06554 zinc transporter, putative similar to zinc
transporter 4; ZnT4 [Mus musculus]
gi|2582990|gb|AAB82593; similar to zinc transporter ZAT
[Arabidopsis thaliana] gi|4206640|gb|AAD11757; member of
the cation diffusion facilitator (CDF) family, or cation
efflux (CE) family, PMID:11500563
Length = 432
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -2
Query: 366 PLALQPGACELRQVHYVRDLHELSVPSDELGSAC 265
P L+ G CE+ +V V +LH ++ +L AC
Sbjct: 362 PTMLEKGVCEIEEVVAVHELHIWAITVGKLLLAC 395
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 27.5 bits (58), Expect = 9.2
Identities = 19/63 (30%), Positives = 29/63 (46%)
Frame = +2
Query: 452 PSNRQGSGGVPARKSPNRLKNKIKKDVVLKVDTENFLSPDTCGGIELVAARGRIKISNTL 631
P + +GG AR N + +D+ L++ TE L GG E V GRI + +
Sbjct: 277 PVLQGAAGGAEARPFVT-FHNSLGRDLYLRIATELHLKRMLVGGFEKVYEIGRIFRNEGI 335
Query: 632 ESR 640
+R
Sbjct: 336 STR 338
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,618,623
Number of Sequences: 28952
Number of extensions: 264737
Number of successful extensions: 739
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 739
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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