Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20821
(713 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 70 3e-13
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 70 3e-13
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 69 5e-13
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 66 5e-12
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 66 6e-12
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 62 1e-10
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 57 3e-09
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 55 1e-08
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 55 1e-08
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 50 4e-07
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 48 1e-06
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 45 9e-06
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 42 7e-05
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 32 0.094
SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|ch... 26 4.7
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 26 4.7
SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 26 6.1
SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pomb... 25 8.1
SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|... 25 8.1
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 70.1 bits (164), Expect = 3e-13
Identities = 33/79 (41%), Positives = 50/79 (63%), Gaps = 1/79 (1%)
Frame = +2
Query: 20 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 196
A +R+ +EL D+ + S V + ++ WQ I+ P + PY G F + I+FP +Y
Sbjct: 2 ALKRINRELADLGKDPPSSCSAGPVGD-DLFHWQATIMGPADSPYAGGVFFLSIHFPTDY 60
Query: 197 PFKPPKISFKTKIYHPNID 253
PFKPPK++F T+IYHPNI+
Sbjct: 61 PFKPPKVNFTTRIYHPNIN 79
Score = 54.8 bits (126), Expect = 1e-08
Identities = 26/87 (29%), Positives = 48/87 (55%)
Frame = +1
Query: 196 PIQTPEN*FQNKDLPP*H**KGQVCLPIISAENWKPATKTDQVIQALVALVNDPEPEHPL 375
P + P+ F + P G +CL I+ + W PA +V+ ++ +L+ DP P+ PL
Sbjct: 61 PFKPPKVNFTTRIYHPNINSNGSICLDILR-DQWSPALTISKVLLSICSLLTDPNPDDPL 119
Query: 376 RAELAEEFLKDRKKFTKNAEEFTKKHS 456
E+A + DR ++ +A E+T+K++
Sbjct: 120 VPEIAHVYKTDRSRYELSAREWTRKYA 146
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 70.1 bits (164), Expect = 3e-13
Identities = 34/94 (36%), Positives = 51/94 (54%), Gaps = 1/94 (1%)
Frame = +2
Query: 17 AATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAE 193
+++RRL KE +D+R+ + R VD+ N+ W + P + Y G F + FP +
Sbjct: 7 SSSRRLTKEYSDLREHPIPDIRVNLVDD-NLFHWACTALGPSDSVYAGGKFHFSLKFPLD 65
Query: 194 YPFKPPKISFKTKIYHPNIDERVRCACPSLVQKI 295
YPF+PP I F T+IYHPN D L Q++
Sbjct: 66 YPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQV 99
Score = 57.6 bits (133), Expect = 2e-09
Identities = 27/88 (30%), Positives = 49/88 (55%)
Frame = +1
Query: 196 PIQTPEN*FQNKDLPP*H**KGQVCLPIISAENWKPATKTDQVIQALVALVNDPEPEHPL 375
P Q P F + P +G VCL I+ + +KP+ K V++ ++ L+ +P P+ PL
Sbjct: 67 PFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRSVLEQILQLLREPNPDDPL 126
Query: 376 RAELAEEFLKDRKKFTKNAEEFTKKHSE 459
A +AE++ DR F K A ++ ++ ++
Sbjct: 127 VASIAEQYRNDRPSFDKIARDYVEQFAK 154
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 69.3 bits (162), Expect = 5e-13
Identities = 31/90 (34%), Positives = 54/90 (60%), Gaps = 1/90 (1%)
Frame = +2
Query: 23 TRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYP 199
+RR+ KEL D++Q + +++ +I +G+ P+ PY G F ++I P +YP
Sbjct: 6 SRRIAKELADVQQDKQAGIQVWTIND-DISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYP 64
Query: 200 FKPPKISFKTKIYHPNIDERVRCACPSLVQ 289
F+PPK++F TKIYHPN+ + C +++
Sbjct: 65 FRPPKMNFDTKIYHPNVSSQTGAICLDILK 94
Score = 45.6 bits (103), Expect = 7e-06
Identities = 19/66 (28%), Positives = 39/66 (59%)
Frame = +1
Query: 259 GQVCLPIISAENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEE 438
G +CL I+ + W P + +L +L+ PEP +P A++A+ +L++ ++F + A E
Sbjct: 86 GAICLDILK-DQWSPVYTMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTARE 144
Query: 439 FTKKHS 456
+T ++
Sbjct: 145 WTSSYA 150
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 66.1 bits (154), Expect = 5e-12
Identities = 28/79 (35%), Positives = 51/79 (64%), Gaps = 1/79 (1%)
Frame = +2
Query: 17 AATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAE 193
A+ + L+K+L +I+++ + F VD+ +I W+ +I+ P++ Y G F ++FP +
Sbjct: 5 ASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQD 64
Query: 194 YPFKPPKISFKTKIYHPNI 250
YP PPK+ F T+I+HPN+
Sbjct: 65 YPLMPPKMKFTTEIWHPNV 83
Score = 36.3 bits (80), Expect = 0.004
Identities = 12/59 (20%), Positives = 31/59 (52%)
Frame = +1
Query: 283 SAENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKKHSE 459
+ E W P + ++ +++++++ P E P + A+EF ++ ++F K ++ E
Sbjct: 107 AGERWLPVHSPETILISVISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVRRSIE 165
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 65.7 bits (153), Expect = 6e-12
Identities = 29/80 (36%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
Frame = +2
Query: 17 AATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPDNPPYNKGA-FRIEINFPAE 193
+ T+RL+ EL + S +SN+L W G I + Y +G F+I ++FPA
Sbjct: 28 SVTKRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPAN 87
Query: 194 YPFKPPKISFKTKIYHPNID 253
YP+ PP I F + ++HPN+D
Sbjct: 88 YPYSPPTIIFTSPMWHPNVD 107
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 61.7 bits (143), Expect = 1e-10
Identities = 32/108 (29%), Positives = 53/108 (49%), Gaps = 1/108 (0%)
Frame = +2
Query: 20 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 196
A RRL KE ++ ++G +E + TW LI PD P+ G + + FP++Y
Sbjct: 6 ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDY 65
Query: 197 PFKPPKISFKTKIYHPNIDERVRCACPSLVQKIGSLLPRLIKLSKH*W 340
P PP + F+ + +HPN+ + C S++ G P + + S W
Sbjct: 66 PLGPPTLKFECEFFHPNV-YKDGTVCISILHAPGD-DPNMYESSSERW 111
Score = 31.1 bits (67), Expect = 0.16
Identities = 9/56 (16%), Positives = 30/56 (53%)
Frame = +1
Query: 283 SAENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEEFTKK 450
S+E W P ++++ ++++++ +P E + + + +DR+++ + +K
Sbjct: 107 SSERWSPVQSVEKILLSVMSMLAEPNDESGANIDACKMWREDREEYCRVVRRLARK 162
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 56.8 bits (131), Expect = 3e-09
Identities = 22/64 (34%), Positives = 37/64 (57%)
Frame = +2
Query: 134 PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDERVRCACPSLVQKIGSLLPR 313
P PY+ G +++ + P+EYP+K P I F +I+HPNIDE C ++ + S +
Sbjct: 39 PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFD 98
Query: 314 LIKL 325
+I +
Sbjct: 99 MINI 102
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 54.8 bits (126), Expect = 1e-08
Identities = 24/53 (45%), Positives = 31/53 (58%)
Frame = +2
Query: 134 PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDERVRCACPSLVQK 292
P Y G F +E+ P EYP PP + F TKIYHPN+D+ R C S ++K
Sbjct: 41 PQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGR-ICLSTLKK 92
Score = 46.0 bits (104), Expect = 5e-06
Identities = 26/92 (28%), Positives = 48/92 (52%)
Frame = +1
Query: 181 LSSRIPIQTPEN*FQNKDLPP*H**KGQVCLPIISAENWKPATKTDQVIQALVALVNDPE 360
L P+ P F K P G++CL + ++W PA + V+ ++ AL+ P
Sbjct: 57 LPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTLK-KDWSPALQIRTVLLSIQALMGAPN 115
Query: 361 PEHPLRAELAEEFLKDRKKFTKNAEEFTKKHS 456
P+ PL ++A+ + ++ + NA E+TKK++
Sbjct: 116 PDDPLDNDVAKIWKENEPQAIANAREWTKKYA 147
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 54.8 bits (126), Expect = 1e-08
Identities = 23/78 (29%), Positives = 44/78 (56%), Gaps = 1/78 (1%)
Frame = +2
Query: 20 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 196
A RRL ++ ++Q V + N++ W +I+ P + P+ G F++ ++F +Y
Sbjct: 5 ARRRLMRDFKRMQQDPPAGVSASPVSD-NVMLWNAVIIGPADTPFEDGTFKLVLSFDEQY 63
Query: 197 PFKPPKISFKTKIYHPNI 250
P KPP + F + ++HPN+
Sbjct: 64 PNKPPLVKFVSTMFHPNV 81
Score = 37.9 bits (84), Expect = 0.001
Identities = 15/60 (25%), Positives = 32/60 (53%)
Frame = +1
Query: 259 GQVCLPIISAENWKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAEE 438
G++CL I+ W P ++ ++ +L+NDP P AE A+ +++K++ + +
Sbjct: 85 GELCLDILQ-NRWSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRK 143
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 49.6 bits (113), Expect = 4e-07
Identities = 27/75 (36%), Positives = 36/75 (48%)
Frame = +2
Query: 29 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPDNPPYNKGAFRIEINFPAEYPFKP 208
R+QK++ D+ S + N+L + I PD Y G F+ I YP P
Sbjct: 27 RIQKDVTDLEIPSTMSTSWPDPIKLNVLHLE--IRPDEGYYKGGKFKFRIQIDDNYPHDP 84
Query: 209 PKISFKTKIYHPNID 253
PK+ KIYHPNID
Sbjct: 85 PKVKCLNKIYHPNID 99
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 48.4 bits (110), Expect = 1e-06
Identities = 24/80 (30%), Positives = 42/80 (52%), Gaps = 2/80 (2%)
Frame = +2
Query: 17 AATRRLQKELNDIRQSGLKSFRDIQ-VDESNILTWQGLIV-PDNPPYNKGAFRIEINFPA 190
++ +RL KE + + ++ V + ++ W+ +I P PY G + ++I+
Sbjct: 3 SSIKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHE 62
Query: 191 EYPFKPPKISFKTKIYHPNI 250
YP PP + F+TKI HPNI
Sbjct: 63 GYPISPPSVYFQTKIVHPNI 82
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 45.2 bits (102), Expect = 9e-06
Identities = 25/71 (35%), Positives = 38/71 (53%), Gaps = 1/71 (1%)
Frame = +2
Query: 20 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 196
A +RL KE ++++ ++ D + NIL W +I P + PY G + + FP +Y
Sbjct: 6 AYKRLMKEYLALQKNPVELV-DAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDY 64
Query: 197 PFKPPKISFKT 229
PFKPP I T
Sbjct: 65 PFKPPAIRMIT 75
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 42.3 bits (95), Expect = 7e-05
Identities = 16/51 (31%), Positives = 31/51 (60%), Gaps = 2/51 (3%)
Frame = +2
Query: 104 NILTWQGLIVPDNPP--YNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNI 250
+++ W+ + +P P + G +++ + FP EYP +PPK F ++HPN+
Sbjct: 37 DLMNWK-VGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNV 86
Score = 41.9 bits (94), Expect = 9e-05
Identities = 20/67 (29%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
Frame = +1
Query: 259 GQVCLPIISAEN-WKPATKTDQVIQALVALVNDPEPEHPLRAELAEEFLKDRKKFTKNAE 435
G VCL I++ E WKPA Q++ + L++DP P + E F KD+ ++ K
Sbjct: 90 GTVCLSILNEEEGWKPAITIKQILLGIQDLLDDPNIASPAQTEAYTMFKKDKVEYEKRVR 149
Query: 436 EFTKKHS 456
++++
Sbjct: 150 AQARENA 156
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 31.9 bits (69), Expect = 0.094
Identities = 14/54 (25%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +2
Query: 95 DESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNID 253
D+ + W I+ P + + + ++I+ A YP PP ++F ++I P +D
Sbjct: 34 DDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVD 87
>SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 703
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +2
Query: 395 NFSKIGRNLQRMQKNLPKNTVKNGLQIKYT 484
NF N+ R K L +T +NG++ KYT
Sbjct: 413 NFKTEDTNVLRYWKTLALSTCENGIEDKYT 442
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 26.2 bits (55), Expect = 4.7
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -3
Query: 234 IFVLKLIFGG--LNGYSAGKLISMRKAPLL*GGLSGTIRPC 118
+ VL+++ G LN +S+ KLI PLL G +R C
Sbjct: 450 LLVLQMLCGAHVLNKFSSLKLIMTHVIPLLPGSYQDLVRRC 490
>SPBC30B4.02c |||R3H and G-patch domain, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +1
Query: 373 LRAELAEEFLKDRKKFTKNAEEFTKKHSE 459
L AEL E++LKD+ K + E K SE
Sbjct: 464 LYAELQEQWLKDKSKKARRRAEREKLRSE 492
>SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1389
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/30 (36%), Positives = 21/30 (70%)
Frame = +2
Query: 38 KELNDIRQSGLKSFRDIQVDESNILTWQGL 127
K+L++ +++ L + I++DE +L WQGL
Sbjct: 50 KQLSESKEAYLDA---IKIDEKAVLAWQGL 76
>SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr
3|||Manual
Length = 828
Score = 25.4 bits (53), Expect = 8.1
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +2
Query: 101 SNILTWQGLIVPDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDER 259
S++ T + LI P PP K E E F+P + ++ I P +D +
Sbjct: 566 SSMQTIESLIHPRFPPLQKHLSPSEFENTFESRFEPVSLENQSHISPPQVDSQ 618
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,794,922
Number of Sequences: 5004
Number of extensions: 56946
Number of successful extensions: 173
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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