Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20815
(704 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 111 4e-25
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 111 4e-25
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 31 0.98
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 3.0
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 3.0
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein 29 3.0
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 4.0
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 29 4.0
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 29 4.0
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 6.9
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 6.9
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 9.2
At1g76010.1 68414.m08825 expressed protein 27 9.2
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 27 9.2
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 27 9.2
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 111 bits (267), Expect = 4e-25
Identities = 47/52 (90%), Positives = 49/52 (94%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 410
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 118
Score = 70.5 bits (165), Expect = 1e-12
Identities = 31/64 (48%), Positives = 44/64 (68%)
Frame = +1
Query: 511 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 690
+PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 212
Query: 691 PLII 702
PL++
Sbjct: 213 PLVV 216
Score = 66.1 bits (154), Expect = 2e-11
Identities = 33/63 (52%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +1
Query: 76 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 249
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 250 GHK 258
GH+
Sbjct: 65 GHQ 67
Score = 37.1 bits (82), Expect = 0.011
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +2
Query: 452 SVAATGVPALVQARGHIIERFPSFP 526
++AAT VPALV ARGH IE P P
Sbjct: 133 AIAATAVPALVMARGHKIENVPEMP 157
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 111 bits (267), Expect = 4e-25
Identities = 47/52 (90%), Positives = 49/52 (94%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 410
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 117
Score = 70.5 bits (165), Expect = 1e-12
Identities = 31/64 (48%), Positives = 44/64 (68%)
Frame = +1
Query: 511 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 690
+PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 211
Query: 691 PLII 702
PL++
Sbjct: 212 PLVV 215
Score = 68.1 bits (159), Expect = 5e-12
Identities = 33/66 (50%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +1
Query: 67 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 240
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 241 KEAGHK 258
K+AGH+
Sbjct: 61 KKAGHQ 66
Score = 37.1 bits (82), Expect = 0.011
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +2
Query: 452 SVAATGVPALVQARGHIIERFPSFP 526
++AAT VPALV ARGH IE P P
Sbjct: 132 AIAATAVPALVMARGHKIENVPEMP 156
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 30.7 bits (66), Expect = 0.98
Identities = 21/70 (30%), Positives = 35/70 (50%)
Frame = +1
Query: 91 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHKPVLN 270
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+ + V
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICIYALGRMRSVWG 408
Query: 271 HGVLDVLSPE 300
LD PE
Sbjct: 409 EDALD-FKPE 417
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 381 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 262
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 381 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 262
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
Length = 320
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/64 (23%), Positives = 32/64 (50%)
Frame = -3
Query: 696 EGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKL 517
E SL +D A F ++ +K + L+ KD+R ++ ++ D++ G + K+
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301
Query: 516 GNLS 505
G ++
Sbjct: 302 GEIN 305
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.7 bits (61), Expect = 4.0
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +3
Query: 216 LEAALLREQGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 386
+ AL+R + G ++ +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMG-AKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 387 TKPW 398
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.7 bits (61), Expect = 4.0
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHKPVLNHGVLDVLSPE 300
P+PF K+P +PD++ H + NSR +C+ K V ++ PE
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCLYSLGRMKSVWGEDAME-FKPE 434
Score = 28.7 bits (61), Expect = 4.0
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHKPVLNHGVLDVLSPE 300
P+PF K+P +PD++ H + NS+ +C+ K V L+ PE
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCLYSLGRMKSVWGEDALE-FKPE 907
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 28.7 bits (61), Expect = 4.0
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -1
Query: 569 CLVLLISWTLSATTRGSSGIFQ*CV 495
CLVLL+S +A T+ SGIF C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 6.9
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -3
Query: 369 HHDTCYRRHPDRTYEYHHHGHAEF 298
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 6.9
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = -2
Query: 145 GAWLHPAPSHSSLSTPTLKVGLPID--SFRYFSEAIPPKY 32
G W+H SH+ S+ L VG+ + Y+S + P +Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 9.2
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -3
Query: 369 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 286
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.5 bits (58), Expect = 9.2
Identities = 17/43 (39%), Positives = 18/43 (41%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 368
QG + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,726,154
Number of Sequences: 28952
Number of extensions: 307832
Number of successful extensions: 1063
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 976
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1051
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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