BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20811 (765 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g22060.1 68414.m02759 expressed protein 29 2.6 At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase f... 29 3.4 At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase f... 28 7.8 >At1g22060.1 68414.m02759 expressed protein Length = 1999 Score = 29.5 bits (63), Expect = 2.6 Identities = 23/80 (28%), Positives = 34/80 (42%), Gaps = 4/80 (5%) Frame = +3 Query: 537 SLEWNVNCDIPNTDYAYSDVLGRCYKMYLTPMTWSEAYRVCSADQSYLAIINTKEEADHL 716 SLE N+ +I + + + + L +W Y C ++ LA + KE + Sbjct: 818 SLEANLQ-NITHENLILLQKIDELESVVLESKSWKTNYETCICEKKELAELMEKEAVEKA 876 Query: 717 VNMTRLAPK----DKVRGKF 764 TRLA D VRGKF Sbjct: 877 HYRTRLATVQAEFDAVRGKF 896 >At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase family protein / zinc finger (MYND type) family protein similar to ubiquitin-specific protease 16 (UBP16) [Arabidopsis thaliana] GI:11993477; contains Pfam profiles PF00443: Ubiquitin carboxyl-terminal hydrolase, PF01753: MYND finger Length = 731 Score = 29.1 bits (62), Expect = 3.4 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = -3 Query: 280 RIQTCHQGKESSPLHFASLLSVHPFL 203 R Q+ + GK S P+HF LL + P++ Sbjct: 537 RFQSDNFGKLSKPIHFPELLDISPYM 562 >At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase family protein / steroid 5-alpha-reductase family protein similar to synaptic glycoprotein SC2 spliced variant from Homo sapiens [EMBL:AF038958], SC2 from Rattus sp. [gi:256994]; contains Pfam 3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544 Length = 310 Score = 27.9 bits (59), Expect = 7.8 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +1 Query: 235 NGGGYFLYPDDKFEFDAVTTYWNTTQPFEWISIGISSQTAKG 360 +G G + P F F+ VT TT+ ++W+ I++QT G Sbjct: 224 SGAGGYQIPRG-FLFNIVTCANYTTEIYQWLGFNIATQTIAG 264 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,315,289 Number of Sequences: 28952 Number of extensions: 343818 Number of successful extensions: 840 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 821 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 840 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1712086600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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