Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20803
(562 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI00015559A0 Cluster: PREDICTED: similar to Methionine... 50 5e-05
UniRef50_A6NC17 Cluster: Uncharacterized protein MARS; n=2; Homo... 48 1e-04
UniRef50_P56192 Cluster: Methionyl-tRNA synthetase, cytoplasmic;... 48 1e-04
UniRef50_UPI0000D56872 Cluster: PREDICTED: similar to CG15100-PA... 47 3e-04
UniRef50_A1ZBE9 Cluster: CG15100-PA; n=9; Bilateria|Rep: CG15100... 44 0.002
UniRef50_UPI00003AF69F Cluster: PREDICTED: similar to methionine... 39 0.069
UniRef50_Q9VG95 Cluster: Glutathione S-transferase D5; n=11; Dip... 38 0.21
UniRef50_A0DHI4 Cluster: Chromosome undetermined scaffold_50, wh... 36 0.49
UniRef50_Q46HR2 Cluster: Glutathione S-transferase zeta class; n... 36 0.85
UniRef50_Q0AKE5 Cluster: Glutathione S-transferase-like protein;... 35 1.1
UniRef50_Q2I0J5 Cluster: Glutathione S-transferase 3; n=3; Obtec... 35 1.1
UniRef50_A4A5U1 Cluster: Glutathione S-transferase family protei... 35 1.5
UniRef50_A6G4D5 Cluster: Putative glutathione S-transferase; n=1... 34 2.0
UniRef50_A5ECC9 Cluster: Glutathione S-transferase-like protein;... 34 2.6
UniRef50_Q8MQC1 Cluster: Putative uncharacterized protein; n=3; ... 33 4.6
UniRef50_Q82VK0 Cluster: Putative uncharacterized protein; n=1; ... 33 6.0
UniRef50_Q550T4 Cluster: Putative uncharacterized protein; n=2; ... 33 6.0
UniRef50_Q4R1I6 Cluster: Glutathione-S-transferase; n=1; Papilio... 33 6.0
UniRef50_Q8NF91 Cluster: Nesprin-1; n=45; Eukaryota|Rep: Nesprin... 33 6.0
UniRef50_P17790-2 Cluster: Isoform 2 of P17790 ; n=4; Gallus gal... 32 8.0
UniRef50_Q3W7K0 Cluster: Cytochrome P450; n=5; Frankia sp. EAN1p... 32 8.0
UniRef50_Q6FM67 Cluster: Similar to sp|P43596 Saccharomyces cere... 32 8.0
UniRef50_A6QXG8 Cluster: Putative uncharacterized protein; n=1; ... 32 8.0
>UniRef50_UPI00015559A0 Cluster: PREDICTED: similar to
Methionine-tRNA synthetase, partial; n=1;
Ornithorhynchus anatinus|Rep: PREDICTED: similar to
Methionine-tRNA synthetase, partial - Ornithorhynchus
anatinus
Length = 761
Score = 49.6 bits (113), Expect = 5e-05
Identities = 34/97 (35%), Positives = 50/97 (51%), Gaps = 8/97 (8%)
Frame = +2
Query: 263 FSSNAAVQYLFPILDMSENGQCQQLQEWEATRLQPSLSAALGK-----SPASDVKQVLKS 427
FS+NA +Y F + ++ QQ EWE T LQP+LSA+L V Q ++
Sbjct: 185 FSTNAICRYFFLLSGWEQDDLTQQWLEWETTELQPALSASLSALVLQGCQGEKVLQPMRR 244
Query: 428 SLEHVNGLLEGHQ--YVLGDKISAADISI-GARYILL 529
+L H++ L ++ GD+ S ADI + G Y LL
Sbjct: 245 ALAHIDHSLSRRSCPFLSGDEESLADIVLWGTLYPLL 281
>UniRef50_A6NC17 Cluster: Uncharacterized protein MARS; n=2; Homo
sapiens|Rep: Uncharacterized protein MARS - Homo sapiens
(Human)
Length = 254
Score = 48.0 bits (109), Expect = 1e-04
Identities = 36/98 (36%), Positives = 53/98 (54%), Gaps = 9/98 (9%)
Frame = +2
Query: 263 FSSNAAVQYLFPILDMSENGQCQQLQEWEATRLQPSLSAAL------GKSPASDVKQVLK 424
FS++A +Y F + ++ Q EWEAT LQP+LSAAL GK DV ++
Sbjct: 60 FSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKK-GEDVLGSVR 118
Query: 425 SSLEHVNGLL--EGHQYVLGDKISAADISI-GARYILL 529
+L H++ L + ++ G+ S ADI + GA Y LL
Sbjct: 119 RALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLL 156
>UniRef50_P56192 Cluster: Methionyl-tRNA synthetase, cytoplasmic;
n=40; Eukaryota|Rep: Methionyl-tRNA synthetase,
cytoplasmic - Homo sapiens (Human)
Length = 900
Score = 48.0 bits (109), Expect = 1e-04
Identities = 36/98 (36%), Positives = 53/98 (54%), Gaps = 9/98 (9%)
Frame = +2
Query: 263 FSSNAAVQYLFPILDMSENGQCQQLQEWEATRLQPSLSAAL------GKSPASDVKQVLK 424
FS++A +Y F + ++ Q EWEAT LQP+LSAAL GK DV ++
Sbjct: 60 FSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKK-GEDVLGSVR 118
Query: 425 SSLEHVNGLL--EGHQYVLGDKISAADISI-GARYILL 529
+L H++ L + ++ G+ S ADI + GA Y LL
Sbjct: 119 RALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLL 156
>UniRef50_UPI0000D56872 Cluster: PREDICTED: similar to CG15100-PA;
n=1; Tribolium castaneum|Rep: PREDICTED: similar to
CG15100-PA - Tribolium castaneum
Length = 926
Score = 47.2 bits (107), Expect = 3e-04
Identities = 28/83 (33%), Positives = 45/83 (54%), Gaps = 1/83 (1%)
Frame = +2
Query: 263 FSSNAAVQYLFPILDMSENGQCQQLQEWEATRLQPSLSAAL-GKSPASDVKQVLKSSLEH 439
F NA V +L+P + + L EWEA L P+L+ + G S ++K L S+L
Sbjct: 56 FVPNAGVWWLWPPAQQAAKAASEYL-EWEAAVLAPTLAHVVGGGSKQENLKACLNSALNK 114
Query: 440 VNGLLEGHQYVLGDKISAADISI 508
+N +LE Y+ K++A+DI +
Sbjct: 115 LNKVLESQPYLSEKKLNASDIVV 137
>UniRef50_A1ZBE9 Cluster: CG15100-PA; n=9; Bilateria|Rep: CG15100-PA
- Drosophila melanogaster (Fruit fly)
Length = 1022
Score = 44.0 bits (99), Expect = 0.002
Identities = 29/84 (34%), Positives = 46/84 (54%), Gaps = 2/84 (2%)
Frame = +2
Query: 263 FSSNAAVQYLFPILDMSENGQCQQLQEWEATRLQPSLS--AALGKSPASDVKQVLKSSLE 436
FS A +YLF + + Q + EW AT L P+L+ A+G ++ VL + ++
Sbjct: 56 FSPAAIAKYLF----VGKGQQRDEWLEWSATLLAPALAHHMAVGHKADANALPVLNALVK 111
Query: 437 HVNGLLEGHQYVLGDKISAADISI 508
++ L+ Y+ GDK SAADI+I
Sbjct: 112 KLDDKLKATPYLAGDKPSAADIAI 135
>UniRef50_UPI00003AF69F Cluster: PREDICTED: similar to
methionine-tRNA synthetase, partial; n=1; Gallus
gallus|Rep: PREDICTED: similar to methionine-tRNA
synthetase, partial - Gallus gallus
Length = 158
Score = 39.1 bits (87), Expect = 0.069
Identities = 30/86 (34%), Positives = 36/86 (41%), Gaps = 4/86 (4%)
Frame = +2
Query: 263 FSSNAAVQYLFPILDMSENGQCQQLQEWEATRLQPSLSAALGKS--PASDVKQVLKSSLE 436
FS NA QY F Q EWEAT LQP+ AAL + L L
Sbjct: 58 FSPNAICQYFFLRRGEQPTDLTTQWLEWEATELQPAALAALYAHLVHGRTGVEALGEPLG 117
Query: 437 HVNGLL--EGHQYVLGDKISAADISI 508
H+ L G Y+ GD S AD+ +
Sbjct: 118 HIERSLVGRGTAYLAGDTESVADVVV 143
>UniRef50_Q9VG95 Cluster: Glutathione S-transferase D5; n=11;
Diptera|Rep: Glutathione S-transferase D5 - Drosophila
melanogaster (Fruit fly)
Length = 216
Score = 37.5 bits (83), Expect = 0.21
Identities = 17/41 (41%), Positives = 27/41 (65%)
Frame = +2
Query: 386 GKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADISI 508
GK + + + ++SS E++N LEG YV GD ++ ADI+I
Sbjct: 119 GKPGSDEDFKKIESSFEYLNIFLEGQNYVAGDHLTVADIAI 159
>UniRef50_A0DHI4 Cluster: Chromosome undetermined scaffold_50, whole
genome shotgun sequence; n=5; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_50,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 444
Score = 36.3 bits (80), Expect = 0.49
Identities = 20/90 (22%), Positives = 40/90 (44%), Gaps = 9/90 (10%)
Frame = +2
Query: 314 ENGQCQQLQEWEATRLQPSLSAAL----GKSPASD-----VKQVLKSSLEHVNGLLEGHQ 466
+ Q Q +W L P+ G PA++ K ++ L+ + G +
Sbjct: 88 QQSQVDQWIDWTINELDPNFMTTFPQLWGHYPANEDTFKTAKNIINDKLKQLEGHFKNSP 147
Query: 467 YVLGDKISAADISIGARYILLLTMMISENS 556
Y++GDK++ AD+++ R ++I E +
Sbjct: 148 YLVGDKLTIADVTLIVRIAPFFILLIDEKT 177
>UniRef50_Q46HR2 Cluster: Glutathione S-transferase zeta class; n=2;
Prochlorococcus marinus|Rep: Glutathione S-transferase
zeta class - Prochlorococcus marinus (strain NATL2A)
Length = 239
Score = 35.5 bits (78), Expect = 0.85
Identities = 15/40 (37%), Positives = 27/40 (67%)
Frame = +2
Query: 410 KQVLKSSLEHVNGLLEGHQYVLGDKISAADISIGARYILL 529
K+ L +LE+++ L+ +++GDK+S ADIS+ + LL
Sbjct: 156 KESLTLNLENLSNLINQENFLIGDKLSIADISVASHLSLL 195
>UniRef50_Q0AKE5 Cluster: Glutathione S-transferase-like protein;
n=2; Hyphomonadaceae|Rep: Glutathione S-transferase-like
protein - Maricaulis maris (strain MCS10)
Length = 214
Score = 35.1 bits (77), Expect = 1.1
Identities = 20/59 (33%), Positives = 34/59 (57%)
Frame = +2
Query: 374 SAALGKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADISIGARYILLLTMMISE 550
S + +S +D + L EH L+G +++ GD+ +AADIS+G Y+L L +I +
Sbjct: 129 SDVIARSAKADTVKALALLAEHG---LKGREFLAGDRFTAADISVG--YMLFLLKIIKQ 182
>UniRef50_Q2I0J5 Cluster: Glutathione S-transferase 3; n=3;
Obtectomera|Rep: Glutathione S-transferase 3 - Bombyx
mori (Silk moth)
Length = 220
Score = 35.1 bits (77), Expect = 1.1
Identities = 16/43 (37%), Positives = 26/43 (60%)
Frame = +2
Query: 386 GKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADISIGA 514
G P + + L+ +L +N LEG +YV GD ++ AD+S+ A
Sbjct: 122 GAKPDAAALKKLEEALVFLNAFLEGQKYVTGDVLTIADLSLVA 164
>UniRef50_A4A5U1 Cluster: Glutathione S-transferase family protein;
n=1; Congregibacter litoralis KT71|Rep: Glutathione
S-transferase family protein - Congregibacter litoralis
KT71
Length = 221
Score = 34.7 bits (76), Expect = 1.5
Identities = 25/64 (39%), Positives = 37/64 (57%), Gaps = 2/64 (3%)
Frame = +2
Query: 341 EWEATRLQP-SLSAA-LGKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADISIGA 514
E A RL P S+S A + + A +V VL + +N L E YV+G+K++ ADI +
Sbjct: 102 ELAARRLIPFSISKAPVPPALAQEVTDVLTKGVAALNSLAEFDPYVMGNKLTLADIYL-- 159
Query: 515 RYIL 526
RY+L
Sbjct: 160 RYVL 163
>UniRef50_A6G4D5 Cluster: Putative glutathione S-transferase; n=1;
Plesiocystis pacifica SIR-1|Rep: Putative glutathione
S-transferase - Plesiocystis pacifica SIR-1
Length = 236
Score = 34.3 bits (75), Expect = 2.0
Identities = 13/45 (28%), Positives = 28/45 (62%)
Frame = +2
Query: 383 LGKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADISIGAR 517
LG ++ + + E + G+L G ++++GD++S ADI++ A+
Sbjct: 160 LGSRSERELVEGAREHFEAIAGMLAGREWLVGDRLSIADIAVAAQ 204
>UniRef50_A5ECC9 Cluster: Glutathione S-transferase-like protein;
n=9; Proteobacteria|Rep: Glutathione S-transferase-like
protein - Bradyrhizobium sp. (strain BTAi1 / ATCC
BAA-1182)
Length = 216
Score = 33.9 bits (74), Expect = 2.6
Identities = 23/82 (28%), Positives = 38/82 (46%), Gaps = 12/82 (14%)
Frame = +2
Query: 302 LDMSENGQCQQLQEWEATRLQPSLSAALG----KSPASDVKQVLKSS--------LEHVN 445
+D +E Q +W + LQP+ + ++P S + ++ L+ V
Sbjct: 86 IDPAERAVADQWMDWSQSALQPAFLGGVFWGWYRTPESKRNMIAVNAALDQTVSYLQRVE 145
Query: 446 GLLEGHQYVLGDKISAADISIG 511
LEG ++LGD +S ADI IG
Sbjct: 146 RQLEGRLFLLGDNLSLADIPIG 167
>UniRef50_Q8MQC1 Cluster: Putative uncharacterized protein; n=3;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 275
Score = 33.1 bits (72), Expect = 4.6
Identities = 17/64 (26%), Positives = 36/64 (56%), Gaps = 4/64 (6%)
Frame = +2
Query: 371 LSAALGKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADIS----IGARYILLLTM 538
++ LGK ++ K++L+ + ++ +L +++ GD+I++ D S IGA + L
Sbjct: 185 VNGVLGKLDVAEQKELLRRDIRAIDDILGDKKFLFGDRITSVDCSVFGQIGAVFYLPYRQ 244
Query: 539 MISE 550
IS+
Sbjct: 245 QISD 248
>UniRef50_Q82VK0 Cluster: Putative uncharacterized protein; n=1;
Nitrosomonas europaea|Rep: Putative uncharacterized
protein - Nitrosomonas europaea
Length = 398
Score = 32.7 bits (71), Expect = 6.0
Identities = 18/51 (35%), Positives = 36/51 (70%), Gaps = 1/51 (1%)
Frame = +2
Query: 329 QQLQEWEATRLQP-SLSAALGKSPASDVKQVLKSSLEHVNGLLEGHQYVLG 478
QQ+++ +ATRL+ ++AA KS A+ + Q L++ ++ V+ L+G++ +LG
Sbjct: 135 QQMEKDDATRLEDVQVAAAALKSAAARIDQ-LRAQIQQVSSTLKGNETLLG 184
>UniRef50_Q550T4 Cluster: Putative uncharacterized protein; n=2;
Dictyostelium discoideum|Rep: Putative uncharacterized
protein - Dictyostelium discoideum AX4
Length = 293
Score = 32.7 bits (71), Expect = 6.0
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +2
Query: 383 LGKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADISI 508
+G +V V KS L ++ LL ++ GD +S ADIS+
Sbjct: 204 VGNFSLDEVVSVFKSDLNSLSNLLGSKTFIFGDHLSMADISL 245
>UniRef50_Q4R1I6 Cluster: Glutathione-S-transferase; n=1; Papilio
xuthus|Rep: Glutathione-S-transferase - Papilio xuthus
Length = 240
Score = 32.7 bits (71), Expect = 6.0
Identities = 18/53 (33%), Positives = 34/53 (64%), Gaps = 1/53 (1%)
Frame = +2
Query: 392 SPASDVK-QVLKSSLEHVNGLLEGHQYVLGDKISAADISIGARYILLLTMMIS 547
SP S+V+ ++L+ +L +N LEG ++ +G ++ AD++I + + TM IS
Sbjct: 127 SPLSEVRFKLLQEALGFLNSFLEGSKFAVGSNMTLADLNI---LVSIETMRIS 176
>UniRef50_Q8NF91 Cluster: Nesprin-1; n=45; Eukaryota|Rep: Nesprin-1 -
Homo sapiens (Human)
Length = 8797
Score = 32.7 bits (71), Expect = 6.0
Identities = 21/57 (36%), Positives = 29/57 (50%)
Frame = +2
Query: 329 QQLQEWEATRLQPSLSAALGKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAAD 499
Q+L EW A QP+++ AL +SP D+ Q L + LE Q +L I AD
Sbjct: 4354 QELMEW-AEEQQPNIAEALKQSPPPDMAQNLLMDHLAICSELEAKQMLLKSLIKDAD 4409
>UniRef50_P17790-2 Cluster: Isoform 2 of P17790 ; n=4; Gallus
gallus|Rep: Isoform 2 of P17790 - Gallus gallus
(Chicken)
Length = 272
Score = 32.3 bits (70), Expect = 8.0
Identities = 16/46 (34%), Positives = 27/46 (58%)
Frame = +2
Query: 386 GKSPASDVKQVLKSSLEHVNGLLEGHQYVLGDKISAADISIGARYI 523
G+ +S K VL ++ L++GH+++LGDK+ D S + YI
Sbjct: 33 GQYSSSADKVVLSCNISAPPTLIKGHKWMLGDKVLKTDESDASSYI 78
>UniRef50_Q3W7K0 Cluster: Cytochrome P450; n=5; Frankia sp.
EAN1pec|Rep: Cytochrome P450 - Frankia sp. EAN1pec
Length = 544
Score = 32.3 bits (70), Expect = 8.0
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = +1
Query: 283 PVSVPDPGHVREWTMPAASRMGGYSPSAVPKCRAREIPSF*RK 411
P S P R W+ PAA+R Y S+ C R PS R+
Sbjct: 77 PASGRSPTRGRPWSPPAAARRPAYCSSSATACEPRRNPSAQRR 119
>UniRef50_Q6FM67 Cluster: Similar to sp|P43596 Saccharomyces
cerevisiae YFR013w IOC3; n=1; Candida glabrata|Rep:
Similar to sp|P43596 Saccharomyces cerevisiae YFR013w
IOC3 - Candida glabrata (Yeast) (Torulopsis glabrata)
Length = 751
Score = 32.3 bits (70), Expect = 8.0
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 507 IEMSAAEILSPRTYWWPSNNPLTCSSDDFKT 415
++ EIL+P Y WP+N P+ D +T
Sbjct: 312 VDQKHKEILTPNIYQWPANEPIDIDQDPLQT 342
>UniRef50_A6QXG8 Cluster: Putative uncharacterized protein; n=1;
Ajellomyces capsulatus NAm1|Rep: Putative
uncharacterized protein - Ajellomyces capsulatus NAm1
Length = 173
Score = 32.3 bits (70), Expect = 8.0
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +1
Query: 184 WKPSLKTMYSQGPVSCRSSRSIQNKVLFQQCGGPVSVPDP 303
W +LKT +S S ++QN V + GG VS+ DP
Sbjct: 101 WLQALKTAAGMSMISMASMEAVQNVVDYHLTGGVVSLSDP 140
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 525,494,695
Number of Sequences: 1657284
Number of extensions: 10285553
Number of successful extensions: 27633
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 26864
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27626
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 37488397230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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