Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20798
(718 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 31 0.22
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 29 0.88
SPAC144.18 |||nucleotide sugar transporter |Schizosaccharomyces ... 28 1.2
SPAC343.13 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 27 2.0
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 27 2.7
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.7
SPAC1952.01 ||SPAC1B3.19|Pig-U|Schizosaccharomyces pombe|chr 1||... 27 3.5
SPAC2E1P3.02c |amt3||ammonium transporter Amt3|Schizosaccharomyc... 27 3.5
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 26 4.7
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 26 4.7
SPAC144.04c |spe1||ornithine decarboxylase Spe1|Schizosaccharomy... 25 8.2
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 30.7 bits (66), Expect = 0.22
Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = -1
Query: 262 GHRTS-DDVSVSGFNSGKGTSGIKSILSEIGRGSSFITG 149
G +T DD+ VSGFN G G+S ++ + + RG + G
Sbjct: 585 GKKTKKDDILVSGFNFGTGSSREQAATAILSRGIPLVVG 623
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 28.7 bits (61), Expect = 0.88
Identities = 15/55 (27%), Positives = 28/55 (50%)
Frame = +3
Query: 231 ETETSSDVRWPIRHRMIKYNSPQPRVNVSEHILFITTSYCDVTSNLVRPYLIYNI 395
ET S D+ I + + S +++ +L I +S C +S L+ P+L +N+
Sbjct: 582 ETIISEDLITTIVNALTNSTSQNKSMHLQVQLLHIISSLCQSSSALILPFLNHNL 636
>SPAC144.18 |||nucleotide sugar transporter |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 345
Score = 28.3 bits (60), Expect = 1.2
Identities = 15/62 (24%), Positives = 29/62 (46%)
Frame = +3
Query: 282 KYNSPQPRVNVSEHILFITTSYCDVTSNLVRPYLIYNINYNEFQFRIPHIWRIYKFSVLA 461
KY P N++ +L + ++ C +++ + INY +F FR W F ++A
Sbjct: 32 KYVLSSPGYNMNFLLLTVQSTVCVAAIGILKRLKV--INYRDFDFREAKFWFPISFLLVA 89
Query: 462 SV 467
+
Sbjct: 90 MI 91
>SPAC343.13 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 526
Score = 27.5 bits (58), Expect = 2.0
Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 4/53 (7%)
Frame = +3
Query: 144 EIPVIKEEPLPISDKMDLIPDVPFP----ELKPETETSSDVRWPIRHRMIKYN 290
EIP+ + +P+S +D +PD P +L+ +T S+ + P R ++ YN
Sbjct: 99 EIPIGRNGFIPLSAGLDNVPDTQIPIQHIQLEQDTAKSTSAKNPSR-ILLDYN 150
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 27.1 bits (57), Expect = 2.7
Identities = 19/65 (29%), Positives = 30/65 (46%), Gaps = 3/65 (4%)
Frame = +3
Query: 195 LIPDVPFPELKPETETSSDVRW---PIRHRMIKYNSPQPRVNVSEHILFITTSYCDVTSN 365
L P+ FP ++P S VR +R + K N+ PR+ V + T CD+ S
Sbjct: 156 LHPNKQFPAIRPFPNEKSLVRHVARQVRLALKKNNNQYPRILVIGALGRCGTGACDLASK 215
Query: 366 LVRPY 380
+ P+
Sbjct: 216 IGIPF 220
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 2.7
Identities = 14/65 (21%), Positives = 34/65 (52%)
Frame = +3
Query: 279 IKYNSPQPRVNVSEHILFITTSYCDVTSNLVRPYLIYNINYNEFQFRIPHIWRIYKFSVL 458
+ + +P P++++ HI+ TS D+ V P + + + ++ + + +IY+ S L
Sbjct: 356 VLWRNPLPQLSIISHIILSPTSMFDIREFNVNPVVSVD-SLKDYSEELVNYAKIYEKSNL 414
Query: 459 ASVKL 473
++L
Sbjct: 415 NCIEL 419
>SPAC1952.01 ||SPAC1B3.19|Pig-U|Schizosaccharomyces pombe|chr
1|||Manual
Length = 408
Score = 26.6 bits (56), Expect = 3.5
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +3
Query: 351 DVTSNLVRPYLIYNINYNEFQFRIPHIWRIYKFSVLASVKLRLYYI 488
D+T NL + + +NEF+ ++ I + V +RLYY+
Sbjct: 244 DLTPNLGLWWYFFTEMFNEFRTFFLFVFAILPLMFVLPVSIRLYYL 289
>SPAC2E1P3.02c |amt3||ammonium transporter Amt3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 517
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/47 (25%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = +2
Query: 458 CFCKIKIILYSLGSSFIYNNKVI*AVYGAISWC--HYYRGISFDVVG 592
CF ++ ++ +++I N +I + +GA++W Y R F +G
Sbjct: 286 CFNSGTLLTVNIRTAYIMTNTLISSSFGALTWAIIDYIRYRKFSTIG 332
>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 809
Score = 26.2 bits (55), Expect = 4.7
Identities = 20/84 (23%), Positives = 39/84 (46%), Gaps = 7/84 (8%)
Frame = +3
Query: 141 PEIPVIKEEPLPISDKMDLIPDVP-FPELKPETET-SSDVRWPIRHRMIKYNSP---QPR 305
P + + EP P D+ DL+ +V P + P ET + +W R + P QP+
Sbjct: 259 PALENEESEPEPSDDQFDLVEEVDVLPNVDPNLETLMASSKWKDRKEALDKLLPVLSQPK 318
Query: 306 VNVSE--HILFITTSYCDVTSNLV 371
+ ++ +++ I T +N++
Sbjct: 319 IKDNDFFNLVAILTKSVSKDANIM 342
>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 993
Score = 26.2 bits (55), Expect = 4.7
Identities = 9/32 (28%), Positives = 19/32 (59%)
Frame = +3
Query: 327 LFITTSYCDVTSNLVRPYLIYNINYNEFQFRI 422
L Y ++T+N+V Y +Y ++ ++ FR+
Sbjct: 198 LIFEDQYIELTTNMVEDYNVYGLSGSQQSFRL 229
>SPAC144.04c |spe1||ornithine decarboxylase Spe1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 25.4 bits (53), Expect = 8.2
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 494 GSSFIYNNKVI*AVYGAISWCHYYRGISFDVVGHFVIVPYEINV 625
GS F+YN+ V + W S DV+ + +PYE+NV
Sbjct: 354 GSRFVYNDLVGTGQHRCFIWGPTCD--SLDVIANDAHLPYELNV 395
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,835,910
Number of Sequences: 5004
Number of extensions: 56856
Number of successful extensions: 141
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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